Objective To study the clinical effect of Xingnaojing injection in treating patients with acute cer-ebral infarction and its influence on serum matrix metalloproteinase 9 (MMP -9),nitric oxide (NO)and nitric oxide synthase (NOS).Methods 112 patients with acute cerebral infarction were randomly divided into two groups by ran-dom number table method,56 cases in each group.The control group received routine treatment for acute cerebral infarction.The observation group received routine treatment plus intravenous injection Xingnaojing.The changes of NIHSS score,motor function score and sensory function score in the two groups were compared before and after treat-ment.The serum levels of MMP -9,NOS and NO in the two groups were observed before and after treatment.Results The total effective rate of observation group was 82.14%,which was significantly higher than 64.29% in the control group (χ2 =4.553,P <0.05).After treatment,NIHSS scores of the observation group were significantly lower than the control group (t =5.082,5.522,6.253,all P <0.01 ).After treatment for 3 weeks,the motor function score (69.54 ±17.82)points,sensory function score (30.58 ±7.94)points of the observation group were significantly higher than (52.93 ±14.66)points and (23.08 ±6.31)points in the control group (t =5.387,5.534,all P <0.01).After treatment,the serum MMP -9,NOS,NO levels of the observation group were significantly lower than the control group (all P <0.01 ).Conclusion Xingnaojing injection in the treatment of acute cerebral infarction has good curative effect,it can promote the recovery of neurological function,improve motor function and sensory function, and by adjusting the MMP -9,NOS,NO levels to protect the nerve and blood vessel function.

Surgical treatment of cirrhotic portal hypertension and its accompanied abdominal diseases is one of the medical problems currently.Accurate evaluation of the hepatic function reserve,degree of portal hypertension and accompanied abdominal disease is important to decrease the postoperative morbidity and mortality as well as to raise the efficacy.The surgical procedure selection should be based on the analysis of portal vein hemodynamics of patients.There are clear indications for portosystemic shunt,devascularization and combinative surgery with shunt and devascularization.Treatment for portal hypertension should be carried out when treating the accompanied abdominal diseases.The principle of operation,operation time,operation mode,operation sequence,staging indications and the key techniques of operation were discussed in the article.Only these principles be mastered,proper selection of surgical procedures and desired treatment effect with minimal trauma can be achieved.

Oxidative stress is involved in cirrhotic portal hypertension and a close relationship exists between oxidative stress and hyperdynamic circulation.This article elaborates on the function of various reactive oxygen species (e.g.,superoxide anion and hydrogen peroxide) and corresponding anti-oxidants (e.g.,NAD(P) H oxidase inhibitor,the scavengers of superoxide anion,hydrogen peroxide,and vitamin C or E) on the occurrence and development of hyperdynamic circulation in cirrhotic portal hypertension.We observed that the level of reactive oxygen species in cirrhotic portal hypertension was elevated over normal.The potential clinical value is that inhibition or removal of these reactive oxygen species can relieve hyperdynamic circulation and reduce complications for cirrhotic portal hypertension.

Early identification and effective treatment of hepatocellular carcinoma plays an important role in improving survival rate.The establishment of simple and effective procedures for early diagnosis, census methods, and effective minimally invasive treatment is an important project in China to improve the diagnosis and treatment level of liver cancer.Contrast-enhanced ultrasound plays an important role in the early diagnosis of liver cancer and radiofrequency ablation treatment.This article describes how contrast-enhanced ultrasound helps with the identification of small hepatocellular carcinoma and degree of tumor differentiation in patients with liver cirrhosis, as well as the differential diagnosis of hyperplasia/canceration and precancerous lesion.It also introduces the important role of contrast-enhanced ultrasound in standard radiofrequency ablation and development of therapeutic regimens and strategies and clarifies that it can be used before, during, and after radiofrequency ablation treatment.

It is related to the treatment effect of the disease and the quality of life of the patient that perioperative management of ruptured esophagogastric varices bleeding with portal hypertension.According to the individual characteristics of the patient combined with the experience of the surgeons,the key points of individualized treatment consist of accurate and comprehensive preoperative preparation,fine post-operative treatment,and the changes of pre-and post-operative hemodynamics of portal vein system to choose a reasonable procedure,meanwhile,the delicate postoperative managements can improve the surgical effect of portal hypertension.

Objective:To observe the effects of osteoprotegerin on osteoclasts in the occlusal tissue during tooth eruption in dog and analyze the mechanism. Methods: Six 7-day-old brood dogs were divided randomly to control and osteoprotegerin groups. The dogs in osteoprotegerin group were injected with osteoprotegerin for 3 days 1.5 mg/(kg?d). 5 days after injection the dogs were sacrificed and mandible were taken out for paraffin slice. The number of the osteoclasts in the occlusal tissue up the third mandibular premolar was detected by tartrate-resistance acid phosphatase(TRAP) staining. Immunohistochemistry was used to examine the expressions of RANKL. Results: The number of osteoclasts in osteoprotegerin group was less than that in the control group (P

Objective To evaluate the radiofrequency ablation in treatment of primary and recurrent liver cancer and to explore the risk factors affecting prognosis.Methods 1 64 patients with primary and recurrent liver cancer underwent radiofrequency ablation treatment.The clinical data including liver function before and after treatment,alpha-fetoprotein (AFP),the rate of relapse and survivalwere retrospectively analyzed.The univariate Logistic regression analysis was used to analyzed the risk factors associated with the prognosis.Then the risk prognostic factors were analyzed using COX risk model.Results After treatment,AFP and liver function were significantly improved in patients (P<0.05);Complete tumor ablation rate was 87.50%.After cancer treatment 1,2,3,4 years,the recurrence rate was 17.9%,29.9%, 45.9% and 55.0% respectively.Conclusion Radiofrequency ablation is an effective treatment for primary liver cancer treatment,the risk factors affecting prognosis include tumor differentiation,tumor recurrence,TNM stage,AFP,intraoperative blood transfusion, surgical,cancer and surgical site before surgery transaminase levels.

Courseware design of Flow cytometry and its clinical application includes aim,contents,methods,teaching effect,key problem and solving measures,etc.It aims to standardize teaching content and overcome the contradiction between more teaching content and less teaching time.It is also an effective way to save limited teaching expense and contributes to making the course of Flow cytometry more concise,lively and concrete.So it can increase overall teaching quality and level.

: Objective To explore the correlation between serum testosterone and type 2 diabetes mellitus in elderly male.Methods 1012 elderly male were divided into 2 groups according to their histry of DM.Blood glucose(FBG,PBG),cholesterol(CHO),triglyceride(TG),blood pressure(DBP,SBP),body mass index(BMI),serum insulin(FINS,PINS),serum testosterone(T)and estradiol(E2)were measured.Results Eldly male with type 2 diabetes showed higher FBG,PBG,TG,FINS,PINS and E2 level than those without DM(P<0.05),and with lower HDL-C and T level(P<0.05).The analysis of multiple stepwise regression showed that T level was correlated with the level of HDL-C,FINS,PINS and TG.Conclusion TG,HDL-C,FINS and PINS level correlate with T level in eldly male with type 2 diabetes mellitus.

Objective To study the influence of laparoscopic radiofrequency ablation combined with intravenous chemotherapy on hepatoma cell growth mediated by PTEN and Wnt pathway. Methods 90 cases of hepatocellular carcinoma patients from May 2013 to June 2016 were chosen as study subjects, all patients were divided into observation group (n = 45), control group (n = 45) randomly. Patients in observation group received laparoscopic radiofrequency ablation combined with intravenous chemotherapy, while those in control group received intravenous chemotherapy only. One week after treatment, two groups patients' liver lesion tissue PTEN signaling pathway, Wnt signaling pathway related gene mRNA expression were detected by RT-PCR method; serum contents of tumor markers were tested by Radioimmunoassay. Results One week after treatment, liver lesion tissue PTENmRNA expression was higher in observation group than that in control group, HIF-1, VEGF mRNA expression were lower in observation group than those in control group, the difference was statistically significant (P < 0.05); liver lesion tissue Wnt pathway related genes mRNA expression such as β-catenin, CyclinD1, c-myc, MMP-7 were lower in observation group than those in control group, the difference was statistically significant (P < 0.05); serum contents of tumor markers such as AFP, GGT-Ⅱ, IGF-2, CA19-9 were lower in observation group than those in control group, the difference was statistically significant (P < 0.05). Conclusion Laparoscopic radiofrequency ablation combined with intravenous chemotherapy can inhibit the growth of HCC cells through the PTEN and Wnt pathway, which has positive clinical significance.