Commonly used tumor markers have poor sensitivity and specificity. A lot of research are being conducted searching for the ideal novel serum markers. There are a large number of tumor-associated autoantibodies in cancer patients's serum. Screening and detecting autoantibodies may be used in the early diagnosis of cancer.

OBJECTIVE:To discuss the feasibility and average infusion velocity of domestic atracurium by continuous in-fusion and to determine and validate its clinical valence.METHODS:80patients undergoing selective operation were treated with domestic atracurium0.5mg/kg to facilitate intubation under the flaccidity monitoring(TOF),the continuous infusion velocity was regulated during operation to keep the level of T 1 at1%～10%of the control value,the infusion was stopped20min before the termination of operation.RESULTS:The average infusion rate was(5.1?0.4)(?g?kg)/min,the drug effect starting time and duration of action of initial dose were(203?47)s and(41.5?4.5)min respectively;Significant correlations were found between the initial time of T 1 and PTC during the TOF reaction-free period and between TR value and DBS value at extubation time(P

Objective To investigate the effect of nerve growth factor(CNF) on the proliferative ability of (human) cholangiocarcinoma cell strain QBC939. Methods After construction of a full-length human ?-NGF expression vector, human cholangiocarcinoma cell QBC939 was transfected with pcDNA3.0-NGF. Western blot assays were used to detect the expression of ?-NGF. Subsequently. In vitro proliferation before and after transfection of cells was analyzed by MTT assays. Results Western blot assay indicated that ?-NGF could be stably expressed by transfected QBC939 cells, and compared with controls, the differences were significant (P

Objective To investigate if cancer cell-derived nerve growth factor participates in regulation of human cholangiocarcinoma cell invasion in vitro. Method After construction of a full-length human p-NGF expression vector, human cholangiocacinoma cell lines QBC939 was transfected with pcDNA3. 0-NGF. Western blot assays were used to detect the expression of ?-NGF. In vitro invasion of transfected cells was analyzed by Transwell assays. Result p-NGF was expressed by transfected QBC939 cells, compared with controls, the differences were significant (P

ObjectiveTo observe the clinical efficacy ofShu Feng An Shen(expelling wind and calming mind) needling plus distal points selection in treating cluster headache.MethodForty patients with cluster headache were divided into two groups by using random number table, 20 cases in each group. The treatment group was intervened byShu Feng An Shenneedling plus distal points selection, while the control group was by acupuncture with conventional points selection. The pain intensity score (Visual Analogue Scale, VAS), lasting duration and attack frequency were observed.ResultThe VAS score, lasting duration and attack frequency were significantly reduced after treatment in the two groups (P<0.01), and the reduction was more significant in the treatment group (P<0.01).ConclusionShu Feng An Shenneedling plus distal points selection can produce a more significant efficacy in reducing pain intensity, lasting duration, and attack frequency in treating cluster headache compared with acupuncture with conventional points selection.

Objective To investigate the clinical efficacy of mind- and qi-regulating acupuncture in treating postoperative gastroplegia.Methods Thirty-six patients with postoperative gastroplegia were randomly allocated to treatment and control groups, 18 cases each. The control group received conventional acupuncture and the treatment group, acupuncture at points for regulating mind and qi (Baihui, Sishencong and Yindang) in addition. Pre- and post-treatment Hamilton Depression Rating Scale (HAMD) scores and gastric drainage volumes, and the number of treatments and the recovery rate were recorded in the patients. Comparisons of them were made between the two groups.Results There was a statistically significant pre-/post-treatment difference in the HAMD score in the two groups (P<0.01). There was a statistically significant post-treatment difference in the HAMD score between the treatment and control groups (P<0.05). There was a statistically significant pre-/post-treatment difference in gastric drainage volume in the two groups (P<0.01). There were statistically significant differences in the number of treatments and the recovery and marked efficacy rate between the treatment and control groups (P<0.05).Conclusions Mind- and qi-regulating acupuncture helps recovery and can also reduce the number of treatments in gastroplegia patients.

BACKGROUND:Less Invasive Stabilization System (LISS) has been shown to hold the same biomechanical strength to proximal femoral nail anti-rotation.OBEJCTIVE:To investigate the safety and effectiveness of the reversed LISS plates for senile femoral intertrochanteric fractures.METHODS:Thirty elderly patients with femoral intertrochanteric fractures were treated with reversed LISS plates.The operation time,incision length and total blood loss (intraoperative plus volume of drainage 1 day postoperative) were recorded.Physiologic and Operative Severity Score for the Enumeration of Mortality and Morbidity (POSSUM) and modified POSSUM scoring systems were used to predict the incidence of complications and mortality,and then the real incidence of complication and mortality was compared to evaluate the safety of reversed LISS plates.The clinical effectiveness was evaluated by fracture healing time and Harris scores at the last follow-up.RESULTS AND CONCLUSION:(1) The incidence of complications showed significant difference between predicted (46.05％) and real (13.3％) results (P=0.05),but the mortality did not differ significantly between predicted (3.93％) and real (0％) results (P=1).(2) The fracture healing time was 21-32 weeks,and the excellent and good rate in Harris scores at the last follow-up was 83.3％.(3) These results indicate that the reversed LISS plates are easy to operate,safe and obtain good efficacy in the treatment of senile femoral intertrochanteric fractures.

Objective:To establish a method for the determination of solvent residues in faeces bombycis extract by GC .Meth-ods: A GC-headspace injection method was adopted to detect the residual organic solvents in the extract of faeces bombycis .A DB-5MS (30 m ×0.25 mm ×0.25 μm) quartz capillary column was used as the chromatographic column;nitrogen was used as the carrier gas at a flow rate of 0.6 ml· min-1;the injector temperature was 200℃;the detector was a flame ionization detector( FID) with the temperature of 250℃;the temperature program included two phases:the initial temperature was set at 40℃for 10 min, and then risen to 200℃at a rate of 5℃· min-1 and maintained for 5 min;the equilibrium temperature of headspace was 95℃;the equilibrium time was 30 min;the capillary temperature was 110℃;the injection ring temperature was 125℃.Results:The linear range of acetone , 2, 3-dimethylpentane, 3-methylhexane, heptane, 2,2-dimethylhexane, p-xylene, m-xylene, O-xylene and 2,4,6-collidine was 101-3 034μg· ml-1 , 100-2 995 μg· ml-1 , 107-3 197 μg· ml-1 , 101-3 019 μg· ml-1 , 99-2 962 μg· ml-1 , 45-1 358 μg· ml-1 , 44-1 325μg· ml-1, 47-1 411 μg· ml-1 and 104-3 130 μg· ml-1, respectively, and the average r was all above 0.992.The recovery of the method met the requirement , and the blank solvent showed no interference .Eight samples were all accordance with the requirements after the examination .Conclusion:The headspace injection method for the determination of residual organic solvents in the extract of faeces bombycis is simple and reliable .

Objective To investigate the effects of He-Ne laser on the treatment of pericoronitis.Methods 75 clinical patients with pericoronitis were randomly divided into the experimental group and control group.Local conventional therapy was given to the two groups.The experimental group was given routine treatment with He-Ne laser local exposure for 15 minutes every day,while the control group took no other treatment.3 days and 7 days later,pain and inflammation degree were recorded by three-degree pain scoring method.Total treatment effects were evaluated under a comprehensive clinical treatment standard.Results After 3 days' treatment,78.9％ patients in the experimental group and 51.4％ patients in control group felt their pain disappeared.After 7 days' treatment,97.4％ patients in experimental group and 91.9％ patients in control group felt their pain disappeared.Treatment comprehensive evaluation showed that there were statistical significance differences between two groups (P＜0.05).Conclusion He-Ne laser treatment of pericoronitis has significant anti-inflammatory analgesic effect.

Objective To investigate the effects of ERα36 (a novel subtype of estrogen receptor alpha) on growth and proliferation in PC12 cells via examining the expression of growth-associated protein in differentiated PC12 cells after ERα36 gene silencing.Methods Transfection of ERα36-shRNA plasmid into PC12 cells was performed to establish the ERoα36 gene silencing cells model (PC12-36L1,PC12-36L2).Immunocytofluorescence was used to examine the expression of ERα36,and Western blot was used to analyze the expression of PCNA,cyclinD1 and MAPK in the PC12 cells.Results ① ERα36 was expressed in both cell types(PC12-36C1,PC12-36L1 and PC12-36L2).Compared with PC12-36C1,PC12-36L2 cells(OD value were respectively 0.95±0.05,0.78±0.10),PC12-36L1 cells significantly decreased expression of ERα36(OD value 0.47±0.12,P＜0.01).② Compared with PC12 and PC12-36C1 cells,PC12-36L1 cells were significantly higher expression of PCNA,CyclinD1 and p-MAPK(P＜0.01)(OD value of PCNA,CyclinD1 and p-MAPK:PC12 cells were respectively 1.00±0.05,1.00± ±0.11,1.00±0.05,PC12-36C1 cells were respectively 1.09±0.15,0.92±0.23,1.12± 0.08,PC12-36L1 cells were respectively 1.74±0.12,2.20±0.25,1.77±0.06).Conclusion ERα36 gene silencing can promote the growth and proliferation in PC12 cells.It suggests that the lower expression of ERα36 may be related to the diseases in nervous system such as brain tumor.