Objective:To explore therapeutic effect of rehabilitation intervention on aged patients with vascular de‐mentia (VD) .Methods :A total of 108 aged VD patients hospitalized in our hospital from Jan 2011 to Oct 2013 were selected .According to random number table ,they were randomly and equally divided into routine treatment group (received routine drug therapy and nursing ) and rehabilitation intervention group [received rehabilitation interven‐tion based on routine treatment group ,including trainings of computing ability ,thinking ability ,memory and activi‐ty of daily life (ADL) ability etc .] .ADL ability score ,mini-mental state examination (MMSE) score ,Wechsler memory scale (WMS) score were assessed ,compared and analyzed between two groups before and one year after in‐tervention .Results:There were no significant difference in ADL ,MMSE and WMS scores before intervention be‐tween two groups (P>0.05) .Compared with before intervention after one -year intervention ,there were signifi‐cant rise in ADL ability score [ (36.87 ± 9.09) scores vs .(47.54 ± 9.72) scores] ,MMSE score [ (16.87 ± 3.55) scores vs .(22.25 ± 3.41) scores] and WMS score [(59.14 ± 6.84) scores vs .(62.88 ± 6.59) scores] in rehabilitation intervention group ( P<0.05 all) ,and they were significantly higher than those of routine treatment group [ADL score :(38.31 ± 8.95) scores ,MMSR score :(16.72 ± 4.25) scores ,WMS score :(59.25 ± 6.11) scores] ,P<0.05 all .Conclusion:Rehabilitation intervention can improve cognitive function and memory and improve self -care a‐bility and quality of life in aged VD patients ,so its therapeutic effect is significant .

Objective Bio-ceramics has been widely utilized as a common type of bone repair material.The research was designed to focus on preparation and characterization of porous TCP.Methods By using hydroxyapatite (HA) as the raw materials,and adding calcium phosphate bio-glass to adjust the ratio between calcium and phosphate,in order to achieve making porous TCP bio-ceramic scaffolds during high temperature heating process.Results Test results showed that by applying this method,TCP scaffolds had significantly higher mechanical strength then those were made from powdered TCP,and its compressive strength reached 9.11 MPa.Conclusion Tri-Calcium Phosphate (TCP) has excellent biocompatibility and it is also biodegradable.In the physiological environment,it is able to be gradually decomposed,absorbed and to promote bone growth ultimately,it is capable of replacing the damaged bone components with brand new bone tissues.

Extra-cellular signals, including T lymphoc ytes, cytokines,growth factors,interferons and neuropeptides, which could modulate matrix metalloproteinases expression in synoviocytes of rheumatoid arthritis(RA), are often mediated by G-protein-coupled Receptors (GPCR)-initiated signaling and mitogen-activated protein kinase (MAPK) cascades. Drug targets which aim at the extra-cellular signals or intra-cellular cascades is required for ameliorating inflammatory reaction and preventing joint destruction in RA.

The harm of tobacco is one of the most serious public health issues nowadays, and the social cost produced by smoking is increasing year by year. There are about 6 million people dying from smoking-related diseases each year, and the number of death will surpass 8 million by 2030. Helping smokers quit is the most direct and effective method to reduce the burden of disease caused by tobacco. M-health based on smartphone shows huge potential in prompting health behavior. Progress of studies on smartphone applications (APP), a new way of tobacco control, is reviewed to provide reference for future tobacco control practice and exploration.

Objective To explore the curative effect of the single pore video-assisted thoracoscopic surgery and thoracic closed drainage for patients with acute pyothorax by comparing the advantage and disadvantage of two methods.Methods The clinical data of 65 patients with acute pyothorax in our hospital from January 2012 to June 2015 were retrospectively analyzed.Of which 30 patients underwent single pore video-assisted thoracoscopic surgery were as observation group,35 cases received thoracic closed drainage were as the control group.The cura-tive effect of two methods for acute pyothorax was compared by analyzing the result data of two groups.Results The postoperative chest tube indwelling time,length of hospital stay,postoperative antibiotics of observation group were significantly less than those of the control group,the difference was statistically significant(P<0.05).And the incidence of postoperative complications,the proportion of the chronic pyothorax and secondary tube rate of observation group were lower than those of the control group,the difference was statistically significant(P<0.05). Conclusion The single pore video-assisted thoracoscopic surgery is safe and effective for treatment of acute pyothorax, which is worth promoting.

Objective To set up the filter mating method of plasmid conjugation experiment for Corynebacterium.Methods The first plasmid conjugation between different genus was performed by filter method.Three strains of highly erythromycin-resistance Enterococcus faecalis isolates[minimal inhibitory concentration(MIC)of erythromycin>256 mg/L,MIC of levofloxacin≤8 mg/L] which screened by agar dilution method were used as donators and 3 strains of Corynebacteria isolates(MIC of erythromycin≤32mg/L,MIC of levofloxacin=128 mg/L)were used as acceptors.In the second conjugation between the same genus but in different species,three Corynebacterium xerosis strains(MIC of erythromycin>256 mg/L,MIC of ceftazidime=16 mg/L)which were conjugants of the first conjugation were used as donators and 6 strains of Corynebacteria isolates(MIC of erythromycin≤32 mg/L,MIC of ceftazidime 128 mg/L)were used as acceptors.Results In the first conjugation between different genus,the plasimid conjugations were done for 9 times between 3 donators and 3 acceptors,4 of which gained the phenotype and genotype of resistance and the transfer rate was 44%.In the second conjugation experiment,the plasmid conjugations were done for 18 times between 3 donators and 6 acceptors,7 of which gained the resistant phenotype and the transfer rate was 39%.Erythromycin-resistance was transferred from the donators to the conjugants in varying degrees.The resistance was transferred from Enterococcus faecalis to Corynebacteria and then to other Corynebacteria which were of the salne genus but of different species.Conclusion Filter mating conjugation method could be used to study the mechanisms of resistance transmission of Corynebacterium.

Objective To explore the clinical effectiveness of urokinase perfusion and drainage in patients with hypertension cerebral hemorrhage broken into ventricles.Methods Patients with hypertension cerebral hemorrhage broken into ventricles(35 cases)underwent urokinase perfusion and drainage.Clinical effect was observed and the nursing point was summarized.Results Of the 35 patients,20 cases acquired good recovery,13 cases were mildly disabled,2 case were severely disabled,no death occurred.The complications mostly consisted of secondary hemorrhage,infection,disfunction.Conclusions The early lateral ventricle drilling operation,postoperative urokinase perfusion and drainage,active complications prophylaxis and treatment,rehabilitation training can reduce mortality and improve the quality of patients' life.

By literature and network investigation,the paper sorts out development modes and typical practices of Internet medical treatment in China,sums up 6 kinds of modes existing domestically,analyzes the advantages and disadvantages of each mode.It is necessary to further probe into suitable operating modes and to give support on aspects of policies,laws and regulations and supervision,and provides reference for better promoting Internet medical treatment in different places.

Objective To study the expressions of matrix metalloproteinase-3 (MMP-3) and osteopontin (OPN) in cervical carcinoma and their relationship with invasion and metastasis. Methods The expression of MMP-3 and OPN were detected in 54 cases of cervical cancer tissues by immunohistochemical SP method. Compared with 15 cases of normal cervical tissues, the relationship was analyzed between both protein expressions and clinical pathology. Results The expression positive rates of MMP-3 and OPN in cervical cancer tissues (70.37 % and 66.7 %, respectively) were significantly higher than those of normal cervical tissues (20 % and 0, respectively), were significantly higher with infiltration depth >1/2 and lymph node metastasis than those with depth≤1/2 and without metastasis , respectively(P <0.05). The expressions of MMP-3 and OPN were related with depth of cervical infiltration and lymph node metastasis of cervical cancer.Expressions between MMP-3 and OPN were significantly positively correlation(r =0.401, P<0.05). Conclusion The expressions of MMP-3 and OPN are correlated with invasion and metastasis of cervical carcinoma.

The establishment animal model of Graves’ disease contributes to the study of etiology, pathogenesis and therapeutic modalities. After decades of studies and making improvements, the method of building mice model of Graves’disease has achieved a great development. Although there were many reports of animal model building in Graves’disease, as a mature technology A-subunit of thyrotropin receptor( TSHR)-expressing adenovirus was used to establish Graves’disease mice model, which has been accepted widely because of its high efficacy.