OBJECTIVE: To provide reference for research and evaluation of core competitiveness of drug research institute. METHODS: Based on the literature review and integrated analysis on the related evaluation methods both at home and abroad, the establishing of evaluation system of core competitiveness of drug research institute was discussed. RESULTS & CONCLUSIONS: The establishment of evaluation system is of great importance to the understanding and improving of the core competitiveness of drug research institute, however, whether the estsablished evaluation system can fully reflect the core competitiveness of drug research institute or not remains to be further tested and improved.

Objective Investigate the diagnosis and treatment of 9 cases in humeral shaft fracture combined with brachial artery injury. Methods 9 cases of humeral shaft fracture combined with brachial artery injury were treated. Results 1 patient was carried out amputation because severe soft tissue injury, obvious limb swelling after operation and occurring thrombosis. The muscles of 2 cases was ischemic and contracture, functional poor because of visiting late. The remaining 6 cases as a result of the diagnosis and treatment in a timely manner, postoperative function were satisfactory. Conclusion Timely diagnosis and treatment for patiends of humeral shaft fracture combined with brachial artery injury can have the good clinical results.

Objective To study the clinical anesthetic effect of butorphanol combined with propofol in painless gastroscopy. Methods 90 patientswith stomachache check were randomly divided into observation group and control group,45 cases each. The observation group was received butorphanol combined with propofol and the control group were treated with fentanyl combined with propofol. The heart rate of pa-tients before anesthesia,during checking and conscious,the change of blood pressure and oxygen saturation,respiratory depression and anes-thetic effect were analyzed. Results The heart rate,blood pressure and oxygen saturation of two groups after treatment were decreased,the difference was not statistically significant(P>0. 05). The total volume of propofol and respiratory depression between the two groups were compared,the differences were statistically significant(P<0. 05). Conclusion Butorphanol combind with propofol in painless gastroscopy with long anesthesia time and low respiratory depression,which was worthy of promotion.

[Objective]To explore the mechanism of bioactive tanshinones in Salviae Miltiorrhiae regulating angiogenesis and lay the foundation for the prevention and treatment of cancer, atherosclerosis, ischemic heart disease and other angiogenesis-related diseases. [Method]Based on angiogenesis mechanisms, infer to recent 10 years of articles from home and abroad, analyze and summarize the angiogenesis regulatory mechanisms of various bioactive ingredients in Danshen on endothelial cells, in vitro tumour cells and in vivo xenograft tumor. [Result] Among various bioactive ingredients of Salvia, Salvianolic acid B, Tanshinone IIA, Cryptotanshinone, Dihydrotanshinone I can promote or inhibit angiogenesis. Furthermore, Salvianolic acid B and Tanshinone IIA are considered to be the most important bioactive ingredients in Danshen and exhibit a dual angiogenic regulating activity through regulating various approaches, such as pro-angiogenic factors, MMPs, HIF-1α, the PI3K/AKT/eNOS signal pathway and so on. [Conclusion]Various bioactive ingredients of Salvia can serve as a regulator of angiogenesis, and it may provide new ideas for the prevention of angiogenesis-related diseases.

The hydrosoluble active constituent of Salvia miltiorrhiza Bge has a wide range of physiological activities and pharmacological effects.Endothelial progenitor cells(EPCs) comprise a cell population that has the capacity to circulate, proliferate and differentiate into mature endothelial cells but that has not yet acquired characteristic mature endothelial markers nor formed precursor cells of blood vessels.It was found that the hydrosoluble active constituent of Salvia miltiorrhiza Bge could protect endothelial functions through modulation of EPCs functions, thus exerting therapeutic effects in endothelium injured related diseases.This paper reviewed the effects of the hydrosoluble active constituent of Salvia miltiorrhiza Bge on EPCs functions.We hope that the paper could provide a reference for further utilization of hydrosoluble active constituent of Salvia miltiorrhiza Bge.

In an uncertain and dynamic environment,medical research institutions must often balance between the usual medical research and emergent medical research.We interpreted this as a balance between exploitation-oriented innovation and exploration-oriented innovation.We pointed out that a flexible organization can coordinate the competition of scientific research resources between the two kinds of innovation; then we built an organizational flexibility model based on resource flexibility and coordination flexibility,and extended the model to medical research institution.And finally,we discussed the strategies for increasing organizational flexibility.

Cyclic alternating pattern (CAP) during sleep is a kind of cyclic oscillation of EEG pattern in non-rapid eye movement sleep(NREM sleep), which reflects microstructure of sleep. It has been identified that CAP plays an important role in keeping the integrity of sleep. The purpose of the interview is to introduce the recording, identification, physiological meaning and clinic implication of CAP.

Objective To evaluate the effects of different doses of dexmedetomidine on the anterograde amnesia of propofol in the patients undergoing gynecological surgery.Methods Sixty patients,aged 20-50 yr,with body mass index of 18-25 kg/m2,of ASA physical status Ⅰ or Ⅱ,scheduled for elective laparoscopic total hysterectomy,were randomly divided into 3 groups (n =20 each) using a random number table:propofol group (group C),low-dose dexmedetomidine combined with propofol group (group D1) and high-dose dexmedetomidine combined with propofol group (group D2).Dexmedetomidine 0.2 and 0.4 mg/kg were infused intravenously over 15 min in D1 and D2 groups,respectively.Target-controlled infusion of propofol was then started at an initial target plasma concentration (Cp) of 0.5 μg/ml.The Cp increased to 0.9,1.3,1.7 and 2.1 μg/ml in turn and maintained at each level for 3 min.After entering the operating room (baseline),at the end of dexmedetomidine infusion,and when Cp was maintained at each level for 3 min after being increased to 0.9,1.3,1.7 and 2.μg/ml in turn,and amnestic test and OAA/S score were performed.The Cp of propofol and BIS value were calculated when the amnestic rate reached 95 ％.The development of hypotension,bradycardia,respiratory depression,and upper airway obstruction was also recorded.Results Compared with group C,the amnestic rate was increased,the Cp of propofol and incidence of respiratory depression were decreased when the amnestic rate reached 95％,BIS value was increased (P ＜ 0.05),and no significant change was found in the incidence of the other adverse events in D1 and D2 groups (P ＞ 0.05).Compared with group D1,the amnesia rate was increased,the Cp of propofol was decreased and the BIS value was increased when the amnestic rate reached 95 ％ in group D2 (P ＜ 0.05),and no significant change was found in the incidence of the other adverse events in D1 and D2 groups (P ＞ 0.05).Conclusion The optimum dose of dexmedetomidine enhancing the anterograde amnesia of propofol is 0.4 μg/kg in the patients undergoing gynecological surgery.

Objective To investigate the teaching effect of heuristic teaching in medical psychology teaching.Methods Totally 104 students (2010 grade higher vocational nursing class) were divided into experiment group (n=52) and control group (n=52).Heuristic teaching model and conventional teaching model were applied respectively.t test was used to compare the final exam scores of two groups; self-made evaluation sheet was used to evaluate the teaching effect and x2 test was employed to compare the results between two groups,with P＜0.05 standing for statistical significance.Results Final exam scores of experiment group (87.48 ± 3.18) was better than control group (81.43 ± 4.89) (P=0.016).Teaching evaluation was better in experiment group than in control group.Conclusions Heuristic teaching is helpful in improving students' study enthusiasm and training their scientific thinking.

ObjectiveThe apoptosis and anti-proliferation effects of photodynamic methods on cervical cancer and ovarian cancer cell lines were studied to explore the advantage of photodynamic therapy in gynecological cancer.MethodsCervical cancer cell line (Hela) and ovarian cancer cell line (SK()V3) were treated with photosensitizer (5-ALA) at different doses and then radiated by laser with energy scopes. The morphological characteristics were observed by light microscopy and electronic microscopy. Inhibition effects and cell apoptosis were studied by MTT assay and flow cytometry (FCM) ResultsThe cytomorphosis and shrinkage, nucleus condensation and anachromasia, dense chromatin lining along cell membrane were observed in both cell line receiving photodynamic therapy.Through transmission electron microscope (TEM), we found nucleus chromatin gathering along membrane of cell nuclear and apoptotic body formed. The cell inhibition rates were 12.9％-90.7％ and 3.9 ％-70.0％, respectively in Skov3 cell and HeLa cell treated with 5-ALA of different concentrations (0.1, 0.5, 1.0, 2.5 mol/L) and receiving laser of different doses (0.1,0.5, 2.5, 12.5 J/cm2). The concentration of the photosensitizer and the dose of laser influenced the optical density(A) of cell lines (P＜0.01). The photo sensitizer was related strongly to laser (P＜0.01). The optical density of the two cell lines decreased with the increases of photo sensitizer concentration and laser dose ( P＜0.01 ),showing concentration- and time-dependent manner.Conclusions Photodynamic therapy inhibits the growth of ovarian cancer and cervical cancer cell line.