Objective To introduce a rapid spot test method for nitrite in water to meet the urgent need of nitrite monitoring during accidental pollution. Methods According to the drinking water legislation in Europe (threshold: 0.10 mg/L NO2-), a rapid spot test method for nitrite in water was established by using an optimized composition of reagent mixture and a binary positive/negative response, conducted as follows: a tablet of a mixture of solid reagents containing sulfanilamide, N-(1-naphthyl) ethylene diamine dihydrochloride and sodium chloride was put on a spot test plate of Teflon mould, then, a drop of 30%(V/V) HCl and a drop of water sample were added on the reagent material. Results Under these conditions, all the test samples containing ≥0.10 mg/L NO2- can produce a positive response of the characteristic red-purple color, whereas, the samples will produce a negative response when the level of NO2- was below the limit. The results of real water samples determined with this new method were confirmed by using the photometric method based on the use of the same reagents. Conclusion The method is simple, rapid , accurate, and suitable for field monitoring of nitrite in water.

Objective: Molecular cloning and sequencing of the human matured fragment of human nerve growth factor(NGF) gene. Methods: Extracting the human genomic DNA from the white blood cells as templates, the gene of NGF was cloned by using PCR and T-vector cloning method. Screening the positive clones and identified by the restriction enzymes, and then the cloned amplified fragment was sequenced and analyzed. Results: DNA sequence comparison the cloned gene of NGF with the GenBank (V01511) sequence demonstrated that both of sequences were identical, 354bp length. Conclusion: Cloning the NGF gene from the human genomic DNA has paved the way for further study on gene therapy of nerve system injury.

Mitofusin 2 (Mfn2) plays an important role in the process of mitochondrial fusion, and is involved in regulating mitochondrial function and morphological changes. Studies have found that Mfn2 has a tumor suppressor effect in a variety of malignant tumors and their cell lines, including cervical cancer, hepatocellular cancer, pancreatic cancer, breast cancer, and bladder cancer. The difference in the expression of Mfn2 in malignant tumor tissues suggests that Mfn2 may play an important role in the proliferation, apoptosis, invasion and migration of malignant tumor cells. As a cancer-related gene, Mfn2 provides new ideas for gene therapy of malignant tumors.

Objective To establish a new model of the Achilles tendon(AT) contraction by stress-shielding. Methods 8 SD rats were randomly divided into two groups, the normal control group and the experimental group. The ATs of the experimental group were stress-shielded by fixing the upper ankle joint in equines position using a cerclage and transecting the sciatic nerve. The control group received no treatment. All rats were killed three weeks later, and all the ATs of the left hind limbs of the experimental and normal control group were tested morphologically by transmission electron microscope (TEM). Results Compared to the normal control group, the collagen fibrils of the experimental tendons did not arrange very well. The number of tendon cells increased, and some of them were pycnotic. Some mitochondrias were swollen. The proportion of the smaller diameter collagen fibril became lager, and small and large fibrils did not distribute in even. Conclusion Our research is successful compared to the past researches and repeatable.

To introduce composition,the testing principle of the urine analyzer,To reduce the failure rate, extend the service life of the instrument and improve the accuracy of diagnosis. Make the maintenance system ,according to the system of equipment maintenance maintain the urine analyzer daily and monthly. The maintenance plays an important role in reducing the failure rate, extending equipment life and improving the accuracy of diagnosis.

Objective　Exploring how to check the proportional hazards assumption of the Cox model,and the solutions to non-proportional hazards between the covariates and the hazard function.Methods　With a example data set of Ⅲc stage ovarian serous cystadenocarcina,illustrating how to use graphical methods to check the proportional hazards assumption of the Cox model.Results　the predictor of post-surgery administering medicine times violated the proportional hazards assumption of the Cox model.Conclusion　when using the Cox model to analyze the predictors of survival time,checking whether the predictors violate the proportional hazards assumption of the Cox model or not should be paid attention to.

Objective To study the clinical effect of surgery combined with antifungal drug in treatment of fungal nose-sinusitis.Methods The clinical data of 108 cases with fungal nose-sinusitis were retrospectively analyzed.All patients were treated with nasal endoscopic sinus surgery.According to whether the patients received antifungal drugs after low-temperature plasma-assisted surgery,the patients were divided into the treatment group and the control group.The treatment group(56 cases) was given antifungal fluconazole after low-temperature plasma-assisted endoscopic sinus surgery.52 cases in the control group were washed by 0.9％ sodium chloride solution after conventional endoscopic sinus surgery.The patients were followed up for 6 months to 2 years,the therapeutic effect and recurrence were compared between the two groups.Results 56 patients of the treatment group,49 cases were cured,7 cases improved,no recurrence.52 cases in the control group,47 cases were cured,improved in 4 cases,ineffective in 1 case,3 cases of recurrence.The recurrence rate of the treatment group was significantly lower than the control group (x2 =4.82,P ＜ 0.05).Conclusion The low-temperature plasma-assisted surgery combined with antifungal drug.in the treatment of fungal nose-sinusitis has exact efficacy,and has significant effect on the prevention and reduce the relapse rate,which worthy of clinical promotion.

Objective To explore the current education situation and demand of network teaching for health service management course so as to provide recommendations for improvement.Methods Questionnaire survey was conducted among 232 undergraduates from majors of preventive medicine,stomatology and clinical medicine.Totally 204 valid questionnaires were organized and recorded into database and SPSS 12.0 was employed to make descriptive statistical analysis of the data.Statistical results were used to show the current education situation and demand of network teaching for health service management course.Results 52.94％ (108/204) of the students interviewed were dissatisfied with current teaching mode.87.25％ (178/204) of the students believed the course website would be beneficial for learning.For the construction of the course website,health insurance system,health policy analysis and health organization system were considered both important and difficult.59.31％ (121/204) of the students showed that the course website should be updated at least once a week.Conclusions It is necessary to explore the network teaching mode for health service management course.Teaching reform depending on different professions and curriculums should be carried out for network teaching.

Objective To investigate the relationship between omentin-1 and Coronary heart disease in different glucose tolerance populations. Methods Serum omentin-1 levels were determined by ELISA in 143 subjects with normal glucose tolerance,impaired glucose tolerance with CHD, type 2 diabetes mellitus with CHD. The relationships between omentin-1 and waist- to-hip ratio, body mass index (BMI), blood Glucose, serum insulin,HOMA-IR and blood fat were also explored.Coronary artery stenosis was screnned by coronary interventional angiography and assessed by Gensini scoring system. Results Serum omentin-1 levels were all lower in T2DM and IGT subjects than those in control subjects (P<0.01). Serum omentin-1 was correlated positively with high density lipoprotein-cholesterol (P < 0.01),and inversely with body mass index,waist-hip ratio,diastolic blood pressure, triglyceride,fasting blood glucose,fasting insulin,HbA1c,[w1]and insulin resistance(HOMA-IR)(P<0.05 or P <0.01).Omentin-1 levels was negative related with Gensini coronary artery score (P < 0.01). Multiple linear regression showed body mass index,HOMA-IR,diastolic blood pressure were independently associated with omentin-1 levels (P<0.05 or P<0.01). Conclusions Serum omentin-1 levels, associated with fat,insulin sensitivity, are likely to play an important role in insulin resistance and the development of CHD in populations with different glucose tolerance.

Objective To study the significance of dissection and exposure of the recurrent laryngeal nerve(RLN) in thyroid surgery.Methods The clinical data of 1 082 patients who had undergone operations on thyroid were retrospectively reviewed.All cases were routinely operated with dissection and exposure of RLN.Results Six cases had injury of RLN(0.5%),3 cases showed parathyroid insufficiency(0.3%)and 2 cases had injury of the superior laryngeal nerve(0.2%) ofter operation.The incidence of operative complications in thyroid surgery was closely related with if exposure of the RLN.Conclusions Exposure of the recurrent laryngeal nerve is a key measure to decrease injury of parathyroids,superior laryngeal nerve and recurrent laryngeal nerve during thyroid surgery.