Atherectomy, Coronary ; methods ; Drug-Eluting Stents ; Humans

Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate,The in- hibiton of cell proliferation,cell cycle distribution,expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay,flow cytometry,immunocytochemistry,and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24,48,72 h after intervention were 2.41?0.48,0.72?0.08,and 0.39?0.04 ug/ml,The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle,enhancement of Caspase-3,and inhibition of Survivin.

Objective To observe the clinical efficacy of Yang Xin Huo Xue(nourishing the heart and activating blood flow) needling method plus Western medication in treating chronic heart failure due to coronary heart disease (CHD).Method Eighty patients with CHD-induced chronic heart failure were randomized into a treatment group and a control group, to respectively receiveYang Xin Huo Xueneedling method plus Western medicationor Western medication alone for intervention. The heart function was estimated and brain natriuretic peptide (BNP) was detected before and after the intervention in both groups, and the change of heart function was observed.Result Yang Xin Huo Xueneedling method plus Western medication achieved a satisfactory therapeutic efficacy in treating chronic heart failure, and its total effective rate was significantly different from the rate in the control group (P<0.05); compared with the control group, the BNP value declined more significantly in the treatment group, and the difference was statistically significant (P<0.05).Conclusion Yang Xin Huo Xueneedling method plus Western medication can produce a satisfactory efficacy in treating CHD-induced chronicheart failure.

A L-serine producing facuhative methylotroph mutant (Mth~H、Gly~R)was derived from Pseudomonas flava A3 by combination treatment with ultraviolet(UV)and diethylsulfate(DES).It could accumulate 6.2g/L L-serine in the medium containing 30g/L glycine and 1% methanol as carbon source when it was cultured for 3 days,and it has an improvement about 67.6% compared with the origin.The mutant also has a good stability of descendiblity of L-serine producing.

Objective To study the effect of caveolin-1 on renal injury and the expression of tight junction proteins in MRL/lpr mice kidney.Methods The mice were divided into 4 groups:5 mice in the normal control group (BALB/c mice);the MRL/lpr lupus mice (n=18) were randomly divided into the MRL/lpr group in which 6 mice were included;the negative control group in which 6 mice were included;the caveolin1 transfection group in which 6 mice were included.The changes of urine protein,the levels of urea (BUN) and creatinine (Cr) were detected.The expressions of claudin-5,occludin,ZO-1 and caveolin-1 protein were determined by western bloting.Analysis of variance was used to determine statistical significant differences between the two groups.A significance level of 0.05 was considered as signigicant.Results Compared with the control group,24 h urine protein [(2 894±437) mg,(412±72) mg],BUN [(8.7±1.5) mmol/L,(6.9±0.4) mmol/L],Cr [(106±22) μmol/L,(85±4) μmol/L] were significantly increased,level of caveolin-1 protein increased (265±17,61±6),the level of occludin (114±12,190±12),claudin-5 (60±5,80±6) and ZO-1 (98±11,206±15) protein decreased in the MRL/lpr group (P＜0.05).After caveolin-1 transfection,the levels of urinary protein [(1 253±249) mg,(2 894±437) mg],BUN [(6.5±1.3) mmol/L,(8.7±1.5) mmol/L],Cr [(78±17) μmol/L,(106±22)μmol/L] were significantly decreased,and the levels of occludin (218±16,114±12),claudin-5 (87±6,60±5)ZO-1 (313±17,98±11) were increased (P＜0.05).Conclusion The expression of caveolin-1 protein in the renal tissues of lupus nephritis increases.Caveolin-1 can reduce the expression of tight junction proteins and contribute to progres-sion of lupus nephritis.

Objective To understand the efficiency of membrane biological reactor (MBR) and sodium hypochlorite generator in a hospital sewage treatment. Methods The samples were collected before and after the sewage was treated by MBR and sodium hypochlorite. The sludge suspend particles, CODCr, BOD5, NH3-N, fecal coliform count and the content of residual chlorine were determined. Results After membrane biological reactor treatment, the removal rates of sludge suspended particles, CODCr, BOD5, NH3-N and fecal coliform was 82.25%, 80.25%, 75.52%, 75.84%, 96.75% respectively. After sodiun hypochlorite disinfection, the removal rate of fecal coliform counts was 99.96%, the content of residual chloride was 3.4 mg/L. Conclusion The efficiency of MBR and sodium hypochlorite is satisfactory in the sewage treatment in the large hospital, the treated sewage will meet the Chinese standard (GB8978-1996).

Rectal carcinoma is a common malignant tumor in China. Although it decreased greatly after the introduction of total mesorectal excision and preoperative radiotherapy, the local recurrence rate of rectal carcinoma after curative surgery is still up to 11.3%. Surgery is the most effective treatment for local recurrence of rectal carcinoma. If excised radically, the five-year survival of patients with rectal carcinoma could be more than 35%. Only a few patients will benefit from ex-tended radical surgery because of its extremely high postoperative morbidity and mortality. Strict selection of patients by mul-tiple preoperative examinations makes extended radical surgery more reasonable. For the unresectable advanced recurrent rectal carcinoma, multimodality palliative therapy could lead to symptom relief, elevated quality of life and prolonged surviv-al. We discuss the diagnosis and treatment of recurrent rectal carcinoma in this review.

Objective To analyze the morphologic characteristics of the single infarct in the territory of perforating arteries which arise from the middle cerebral artery (MCA),and further to investigate the association between the type of cerebral infarction and the isolated stenotic disease of the ipsilateral MCA.Methods Fifty-five patients with acute ischemic stroke who were admitted to the Department of Neurology,Peking Union Medical College Hospital,Beijing,China during January 1,2005 and December 31,2006 were identified.All of the patients had a single acute infarction in the territory of the MCA perforating arteries revealed on diffusion weighted imaging (DWI).Transcranial Doppler (TCD) and magnetic resonance angiography (MRA) were performed for all patients.Patients with stenesis >50% of proximal internal carotid artery and potential cardiac sources of embolism were excluded from the study.Patients were classified into 2 groups according to the presence of the atberosclerotic stenosis of the ipsilateral MCA: patients with or without MCA stenosis.Size of the lesions was measured including the diameter,area and volume.The infarcts with a diameter less than 2 cm were classified as lacunar infarcts; those bigger than 2 cm were classified as the striatocapsular infarcts.The infarcts on DWI was seen in basal ganglia,the body of lateral ventricle and beth.The concomitance of subcortical multiple small old infarcts or leucoariosis on T2>WI-MRI between the two groups was assessed.Results Among these 55 patients,14 (25.5%) had stenosis of the ipsilateral MCA and 41 (74.5%) had a normal MCA.In the group of MCA stenosis,71.4% patients were lacunar infarcts; 67.3% patients were also lacunar infarcts in the normal MCA group.There was no significant difference between the two groups (χ2=0.147,P=0.701).No significant difference in the diameter,area and vo|ume of the infarcts was found between the two groups.The basal ganglia,the body of lateral ventricle and both of these places involvement accounted for 31.7% ,17.1%,51.2% in the normal MCA group; 35.7%,28.6%,35.7% in the stenotic MCA group.No significant difference was found (χ2=1.272,P=0.529).Twenty-three (56.1%) patients had concomitant of small old subcortical multiple infarcts or leucoariosis in the normal MCA group and 3 (21.4%) in the MCA stenosis group,and there was a significant difference between the two groups(χ2=5.033,P=0.025).Conclusions A series of pathologic mechanisms are supposed to cause the single infarction in the territory of MCA perforating arteries.Stenosis of MCA is an underlying cause in addition to perforating artery diseases.There is no significant difference in the size,volume or distribution of the lesions between the two groups.The concomitant of subcortical multiple small old infarcts or leucoariosis may demonstrate the perforating artery disease.

Exploring the functional network during the interaction between emotion and cognition is an important way to reveal the underlying neural connections in the brain. Sparse Bayesian network (SBN) has been used to analyze causal characteristics of brain regions and has gradually been applied to the research of brain network. In this study, we got theta band and alpha band from emotion electroencephalogram (EEG) of 22 subjects, constructed effective networks of different arousal, and analyzed measurements of complex network including degree, average clustering coefficient and characteristic path length. We found that: (1) compared with EEG signal of low arousal, left middle temporal extensively interacted with other regions in high arousal, while right superior frontal interacted less; (2) average clustering coefficient was higher in high arousal and characteristic path length was shorter in low arousal.
Arousal ; Bayes Theorem ; Brain ; physiology ; Brain Mapping ; Cluster Analysis ; Cognition ; Electroencephalography ; Emotions ; Humans

Colorectal neuroendocrine neoplasm (NEN) is originated from colorectal neuroendocrine cells.The incidence of colorectal NEN is low,however it has been increasing in recent years.As the research work has gone further,WHO has graded colorectal NEN to neuroendocrine tumor,neuroendocrine carcinoma,mixed gland-neuroendocrine carcinoma and hyperplastic and pre-neoplastic lesions.Although colorectal NEN cells can produce hormones and have endocrine functions,the clinical symptoms may not be shown.The diagnosis and treatment for colorectal NEN are different according to different classifications and stages.