Objective:To construct a vector containing human vascular endothelial growth factor,VEGF 121 (VEGF 121 ) with green fluorescence protein as the reporter gene,and then to observe its expression in osteoblast.Methods:VEGF 121 gene was amplified from foetus brain library by PCR,then ligated with pEGFP-C1 to form recombinant plasmid pEGFP-VEGF and transfected into osteoblast by liposome.The green fluorescence protein expression was observed by fluorescence microscopy and the VEGF expression was last detected by immunocytochemistry and ELISA.Results:VEGF 121 gene fragment was amplified by PCR and ligated to pEGFP-C1,then transfected into osteoblast successfully.The strong expression of green fluorecensce protein was observed by fluorecensce microscopy,and the expression of VEGF protein was detected by immunocytochemistry and ELISA.Conclusion:Recombinant plasmid pEGFP-VEGF is effectivly expressed after being transfected into osteoblast in vitro,which provides theoretical support for further study with its simple and reliable detecting methods.

Adaptor Proteins, Signal Transducing ; genetics ; Bone Marrow Diseases ; genetics ; pathology ; DNA Mutational Analysis ; DNA-Binding Proteins ; genetics ; Exocrine Pancreatic Insufficiency ; genetics ; pathology ; Glucose-6-Phosphatase ; genetics ; Humans ; Leukocyte Elastase ; genetics ; Lipomatosis ; genetics ; pathology ; Mutation ; Neutropenia ; congenital ; genetics ; Proteins ; genetics ; Transcription Factors ; genetics

Child ; Complement C3 ; metabolism ; Cytokinins ; analysis ; metabolism ; Female ; Humans ; Immunoglobulin A ; immunology ; Immunoglobulin G ; immunology ; Interferon-gamma ; immunology ; Interleukin-12 ; analysis ; immunology ; Interleukin-2 ; analysis ; immunology ; Male ; Peptide Fragments ; immunology ; Respiratory Tract Infections ; epidemiology ; immunology ; virology ; Secondary Prevention ; Tuberculin ; analysis

Objective To explore the role of estradiol decline in the early luteal phase (the 2nd day after oocyte retrieval) in the prediction of in-vitro fertilization outcomes. Methods A total of 236 cases under in vitro fertilization-embryo transfer (IVF-ET)/ intracytoplasmic sperm injection-embryo transfer(ICSI-ET) cycles were included in this retrospective study of their medical records. The cases were divided into three groups according to the levels of estradiol decline on the 2nd day following OPU: group A (n = 70) with the decline rate of less than 70%, group B (n=114) with the decline rate of 70%~80%and group C (n=52) at the decline rate of more than 80%. The comparisons were done among the three groups in terms of the number of oocyte retrieval, the rate of fertilization, the rate of best oocytes and the rate of miscarriage. Results There were no significant differences statistically in the number of oocyte retrieval, the rate of fertilization, the rate of best oocytes and the rate of miscarriage (P > 0.05). But the rates of clinical pregnancy rate in groups A and B were significantly higher than that in group C (68.6% and 68.4% vs. 44.2%) (P < 0.05). Conclusion The levels of estradiol declines in the early luteal phase may be important in the prediction of IVF outcomes.

Objective: To study characteristics of children's EEG complexity at different ages and in different physiological states.Methods: The continuous 24 hour EEG recordings were obtained from 16 electrodes in 45 essential healthy children between the ages of 0 to 15 years. EEG complexity was analyzed by non linear measure in 7 states: awake with eyes opened, awake with eyes closed, NREM (nonrapid eye movements) sleep including stages ⅠandⅡ (light sleep), ⅢandⅣ(deep sleep) and REM (rapid eye movements) sleep. Meanwhile, the correlation was analyzed between complexity and ages.Results: (1)The global EEG complexity in state of being awake with eyes opened was greater than that with eyes closed;that in wakefulness state was greater than in sleep state. The EEG complexity gradually decreased with the increase of deep sleep in NREM sleep state.The complexity in REM sleep state was greater than that in deep sleep state, but lower than in wakefulness state. (2) The global EEG complexity was positively related to ages in state of being awake with eyes opened, state of being awake with eyes closed, light sleep, and not related to ages in deep-sleep state and REM sleep state.(3)In every brain area EEG complexity was positively related to ages in state of being awake with eyes closed .In paracentral region EEG complexity was positively related to ages in states of being awake with eyes opened and light sleep.Conclusion: The EEG complexity was used to study the brain dynamical characteristics in different physiology states and the relationship between encephalic electric activity and brain development. It can be used as an objective index to evaluate the function and development of brain.

To explore the cognitions and behavior of cough etiquette of 556 residents in Longhua Community and self-compare the changes of cognitions and behavior before and after health education and training.The results showed that awareness rate of cough etiquette was low before training (senior group:25.4％,children:11.9％,young white-collar:36.1％,immigrants:14.3％,P ＜ 0.05) and increased significantly (senior group:97.2％,children:99.2％,young white-collar:100.0％,immigrants:98.6％,P ＜ 0.05).Except for no difference in compliance aspects of immigrants (P ＞ 0.05),the other three groups improved after training.And the difference was statistically significant before and after training (P＜0.05).

Gene expression profile technology is characterized by high automation, high accuracy and large information volume, which provides a new way for the development of individualized therapies and novel therapeutic targets. Some researches have identified sets of discriminating genes by using gene expression profile technology that can be used for the predietion of response to radiotherapy in human cancers. Some studies also have confirmed the therapeutic tagerts of radiation sensitizer.

Total 2625 cases of pulmonary sequestration were collected from literatures of Chinese National Knowledge Infrastructure from 1998 to 2008. The age, gender, clinical symptoms, chest CT scan,chest X-ray findings, lesion location, supplying artery, venous reflux, and preoperative misdiagnosis of 2625 cases were retrospectively reviewed. The male: female ratio of all cases was 1.58: 1. The main clinical symptoms of pulmonary sequestration were cough, sputum, fever, hemoptysis and chest pain, but 13.36% patients were asymptomatic. Chest CT scan showed pulmonary massive lesions (49.00%), pulmonary cystic lesions (28.57%), pulmonary cystic-solid lesions ( 11.57% ), and flaky shadows (7.96%). Pulmonary sequestration mainly located in the lower lobe; 66. 43% located in the left posterior basal segments, and 20. 16% located in the right posterior basal segments. Pulmonary sequestrations were divided into three types, intralobar sequestration (83. 84% ), extralobar sequestration ( 16.03% ), and mixed sequestration (0.13% ). The arterial supply was mainly from branches of thoracic aorta (76. 55% ) and abdominal aorta (18.47%). The clinical manifestations of pulmonary sequestration were various, so the preoperative misdiagnosis rate was as high as (59 ± 20) %. We should take full advantage of clinical symptoms, imaging findings and location characteristics to improve the preoperative diagnosis rate.

Objective To investigate the secondary metabolites of the South China Sea sponge Acanthella cavernosa. Methods The acetone extract of A. cavernosa was purified by repeated column chromatography on silica gel, sephadex LH-20, and high-performance liquid chromatography (HPLC). Structures of the extract were determined by spectroscopic analysis and comparison with previously reported data. Results and Conclusion A total of 7 compounds were isolated and their structures were identified as kalihinene X-Z (1 - 3), (R)-3,5-diethyl-5-((S,E)-2-ethylhex-3-en-1-yl)furan-2(5H)-one (4), methyl(2Z,6R,8S)-4,6-diethyl-3,6-epoxy-8-methyldodeca-2,4-dienoate (5), methyl(2Z,6R,8R,9E)-3,6-epoxy-4,6,8-triethyl-2,4,9-dodecatrienoate (6), and methyl(2Z,6R,8S)-3,6-epoxy-4,6,8-triethyldodeca-2,4-dienoate (7); and compounds 4 - 7 were identified from the genus Acanthalla for the first time.

A high incidence of esophageal cancer exists in China. Surgical resection remains the dominant therapeutic intervention for patients with operable esophageal carcinoma. However, alternative strategies are actively applied to reduce the frequency of post-op-erative local or distant disease recurrence and to prolong survival. These strategies include neoadjuvant and adjuvant therapies. This review discusses the current knowledge, as well as available data and information, with regard to neoadjuvant and adjuvant therapies for patients with locally advanced esophageal cancer.