The ischemic myocardium in cultured myocardial cell of newborn rat and treated with Sodium Selenite(Se) and Fructose-1, 6-diphosphate(FDP) were studied comparatively by means of morphometry and quantitative cytochemistry. The area of myocardial cell and mitochondrion and the content of succinic dehydrogenase (SDH) in myocardial cell were quantitated by texture analysis system (TAS). Lipid peroxidation(LPO) and the myocardial cell beat function were observed. The results showed that the changes of the mitochondrion area interrelated with its number remarkabiy. When the mitochondrion area enlarges more than two fold, the destruction of mitochondrion is remarkable. Both Se and FDP can protect the ischemic myocardial cell, but the effects Se on forestalling destruction of mitochondrion, maintaining vitality of SDH and reducing content of malondialodehyde (MDA) are more powerful than FDP, and the effects of FDP on reducing edema of myocardial cell and mitochondrion, improving myocardial cell beat function are better than Se.

Palmer-foot hyperhidrosis is one kind of focal hyperhidrosis, with symptoms of sweating hands or feet. Some people even have sweat dripping off their hands or feet. This article studied the views of TCM on palmer-foot hyperhidrosis, expounded the pathological and physiological relationship between palmer-foot hyperhidrosis and spleen and stomach, and proposed that clinical treatment should be based on the syndrome differentiation and treatment of spleen and stomach, which can achieve certain efficacy.

China ; epidemiology ; Humans ; Neoplasms ; epidemiology

Accidents, Occupational ; mortality ; Animals ; Cause of Death ; Humans ; Male ; Nitriles ; poisoning ; Rats

Objective To investigate the difference between contrast sensitivity,noise and crowding phenomenon in normal and ametropic amblyopia children. Design Prospective case series. Participants 50 normal children and 50 ametropic amblyopia children. Method Using the noise visual acuity test chart,crowded visual acuity chart and contrast sensitivity visual chart of the visual perception system developed by the National Medical Care Apparatus Engineering Research Center,normal and ametropic amblyopia children were examined in Beijing Tongren Hospital. Main Outcome Measures The number and the constituent ratio of normal and abnormal children in contrast sensitivity,noise and crowding phenomenon. Results The abnormal results of contrast sensitivity in ametropic amblyopia children and normal children were 60 eyes (60%) and 15 eyes (15%) respectively; the abnormal result of noise visual acuity were 62 eyes (62%) and 30 eyes (30%) respectively; the crowded visual acuity were 56 eyes (56%) and 0 eye respectively (all P=0.000). In mild,moderate and severe ametropic amblyopia children,the abnormal results of contrast sensitivity were 32 eyes (51.61%),20 eyes (66.67%) and 8 eyes (100%) respectively (P=0.005),the abnormal results of crowding phenomenon were 30 eyes (48.39%),18 eyes (60%) and 8 eyes (100%) respectively (P=0.010),the abnormal results of noise were 39 eyes (69.90%),18 eyes (60.00%) and 5 eyes (62.5%) respectively (P=0.868). In mild,moderate and severe refractive error children,the abnormal results of contract sensitivity were 33 eyes (70.21%),9 eyes (34.12%) and 17 eyes (62.96%) respectively (P=0.011),the abnormal results of noise were 30 eyes (63.83%),15 eyes (57.69%) and 17 eyes (62.96%) respectively (P=0.868),the abnormal results of crowding phenomenon were 30 eyes (63.83%),11 eyes (42.31%) and 17 eyes (62.96%) respectively (P=0.172). Conclusion Contrast sensitivity,noise and crowding phenomenon in visual perception tests can be used to estimate the impairment of visual perception in amblyopia children.

Aim To investigate the changes of [Ca2+]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT.Methods PAH rat model was established by MCT intraperitoneal injection.The PASMCs were primarily cultured and loaded with Fura-2/AM.Effects of Ryanodine receptor agonists on intra-cellular calcium were measured by Fluorescence microscopy Results After being given 10 nmol?L-1 Ryanodine,the concentration of [Ca2+]i in control group increased by(93.31?12.41)nmol?L-1;and the concentration in PAH group increased by(141.71?13.59)nmol?L-1(P

Objective To evaluate the independent effect of premorbid statins on incident ischemic stroke severity and outcome at discharge.Methods We included consecutive patients with ischemic stroke and recorded demographic data,vascular risk factors,Oxfordshire Classification,the method of treatment National Institutes of Health Stroke Scale(NIHSS)score,previous statins treatment,and outcome discharge.We analyzed the data using univariate methods and a logistic regression with the dependent variable of good outcome(Barthel Index,BI 95 to 100).Results A total of 185 individuals met study criteria:57 patients were on statins agents prior to admission and 128 were on no statins.Pretreatment with statins was associated with lower presenting median NIHSS(6.0 vs 13.0,P

Objective To investigate the clinical effect and safety of Epalrestat,an aldose reductase inhibitor(ARI),on patients with diabetic neuropathy.Methods 80 patients with diabetic neuropathy were randomly divided into two groups,one group were treated with Epalrestat,the other was treated with VitB12 for 16 weeks.All patients were measured on their symptoms,the physical signs and SNCV of medial nerve,ulnar nerve,peroneal nerve and tibial nerve during the treatment.Results The degrees of improvement were similar between the two groups.The subjective symptom and physical signs were significantly improved(P

The cytotoxic effect and antitumor effect of paclitaxel liposome were studied by using MTT assay method in vitro and in vivo antitumor test. The inhibitory rate of paclitaxel liposome on human ovarian cancer cell COC1 was 78.48 %, 84.90 %, 93.53 % respectively at the concentrations of 22.5 μg/ml, 45 μg/ml, 90 μg/ml respectively. Under the doses of 5 mg/(kg*d) and 10 mg/(kg*d) for 8 days, the inhibitory rate of paclitaxel liposome against xenograft of solid Ehrlich tumor in mice was 32.47 % and 54.63 % respectively (P<0.01). The weight change rate of the mice was 22.11 % and 21.16 % respectively. It was suggested that paclitaxel liposome possessed in vitro and in vivo antitumor activities. The toxicity of liposomal paclitaxel was lower than that of free paclitaxel.

As the rapid development of genomics , functional genomics and information technology , precision medicine came into being after evidence-based medicine .Precision medicine not only urges clinical doctors to analyze disease at a molecular level to a -chieve precision diagnosis , but also requires us to seek the best therapeutic targets according to the patient 's genetic information to a-chieve precision treatment .The United States and China have launched their precision medicine initiative in 2015 , thus the precision medicine era is coming .This review will focus on gene sequencing , big data integration , molecular pathology , non-invasive prenatal precision diagnosis , and precision cancer medicine to elucidate the application of precision medicine concept in clinical practice .