Objectives:To study the localization of dehydroepiandrosferone (DHEA) in rat gastric epithelial cells.Methods:The immunohistochemical SABC was used.Results:The parietal cells in gastric epithelial cells appeared DHEA positive immune reaction.The positive substance was distributed in the cytoplasm and negative in the nuclei.Conclusions:Parietal cells had DHEA,which might play an important role in the regulation of digestive function.

OBJECTIVE:To study the effects of arsenic trioxide(As 2 O 3 )on the encoding protein expressions of nm23and P53in gastric cancer cells and to study the anti-canecer mechanism of which in the tumor.METHODS:A control group and a test group were set up.The expressions of gene coding proteinum nm23and P53of the gastric cancer cells cultured in vitro by the action of As 2 O 3 were determined by immunohistochemistry method.RESULTS:The expression levels of nm23and P53in gastric cancer cells have been lowered compared with the control group.CONCLUSION:As 2 O 3 can exert its anti-tumor effect by decreasing the expression of gene coding proteinum of nm23and P53.

OBJECTIVE To investigate vascular endothelial growth factor-C (VEGF-C) and cyclooxyegense-2(COX-2) expression in nasopharyngeal carcinoma. METHODS The expression of COX-2 and VEGF-C were stained respectively in nasopharyngeal carcinoma tissues of 45 patients using immunohistochemical method,and were analyzed with clinical data. RESULTS The positive expression rates of COX-2 and VEGF-C were 68.9 % and 53.3 % respectively in nasopharyngeal carcinoma tissue. The expression of COX-2 was positively correlated with VEGF-C (r=0.334,P

Objective To investigate the value of DNA-image cyt-ometry (DNA-ICM) in the diagnosis of urothelial cell carcinomas (UCC). Methods Totally 162 voided urine specimens (92 cases from urothel-ial car-cinomas patients and 70 cases from benign urinary system diseases patients ) were detected with DNA-ICM and liquid-based cytology (LBC), respectively. Results The sensitivity and specificity of DNA-ICM were 65.2%and 100% respectively in the diagnosis of UCC but those of LBC were 27.2% and 98.6%, respectively. The sensitivity of DNA-ICM was significantly higher than that of LBC in the diagnosis of UCC (P < 0.01). The sen-sitivity of DNA-ICM in upper urinary tract urothelial cell carcinomas (UTUC) were 77.1%, which was much higher than that in bladder urothelial cell carcinomas (57.9%) but no statistical significance was found (P >0.01). Conclusion DNA-ICM, which improves the positive rate of urinary cytology, has great application value in the diagnosis of urothelial cell carcinomas and it is an effective screening method for urothelial cancer in diag-nosis and follow-up.

Objective To summarize the treatment experiences and long-term surgical efficacy of spinal hemangioblastomas.Methods The surgical experiences of 46 patients with spinal hemangioblastomas were analyzed retrospectively.All patients underwent microsurgical resection without preoperative embolization, and 52 operations were performed and 58 tumors were resected.McCormick classification was used to evaluate the function of spinal cord.The mean follow-up time was 8 years.Results Total tumor resection was achieved in 55 tumors and subtotal ones in 3.A week later, improvement was obtained in 11 cases, no changes in 29 and aggravation in 6.No patients died.During a follow-up period from 7 to 9 years, the spinal function was improved in 18 patients, remained stable in 21 and deteriorated in 7.No tumor recurrence was found.The subtotal resection affected the prognosis (P =0.08, OR =10.8, 95 ％ CI 2.1-60.8).Conclusions The spinal hemangioblastomas are highly vascularized benign tumors that can be surgically cured under microscope.Subtotal resection is a risk factor of prognosis.

Objective To evaluate the clinical value of microvascular anastomotic devices in the reconstruction of lower extremity defects by free flap transfer.Methods A retrospective review of 42 consecutive patients who underwent lower extremity microvascular reconstruction performed from May 2013 to November 2013 by microvascular anastomotic devices was performed.Patient charts were reviewed for age,sex,etiology of defect,location of defect,flap type,anastomotic technique,complications and flap survival.Results No patients had an arterial or venous anastomosis revised.The vascular anastomosis patency rates was 100％ and the flap survival rate was 97.6％.Total complication rate (9.5％) was due to 1 partial flap loss,1 partial skin graft loss and 2 hematoma.There were no intraoperative or perioperative complications involving the use of a microvascular anastomotic device itself.Conclusion Microvascular anastomotic devices have effective vessel anastomoses in lower extremity microvascular reconstruction.Thus,it presents an ideal tool for lower extremity microsurgical reconstruction.

OBJECTIVETo compare the effect of transurethral resection of the prostate combined with endocrine therapy (TURP + ET) with that of αlA-blockers combined with ET ((αlA-b + ET) in the treatment of bladder outlet obstruction (BOO) in patients with advanced prostate cancer (PCa), and to investigate the safety of the TURP + ET for the treatment of PCa with BOO.

METHODSWe retrospectively analyzed 63 cases of PCa with BOO, 28 treated by αlA-b + ET and the other 35 by TURP + ET. We obtained the residual urine volume (RV), maximum urinary flow rate (Qmax), International Prostate Symptom Score (IPSS), and quality of life score (QoL) before and after treatment along with the overall survival rate of the patients, followed by comparison of the parameters between the two methods.

RESULTSAt 3 months after treatment, RV, IPSS, and QoL in the TURP + ET group were significantly decreased from (137.8 ± 27.6) ml, (22.3 ± 3.6), and (4.2 ± 0.8) to (29 ± 13.6) ml, (7.8 ± 2.1), and (1.6 ± 0.5) respectively (P < 0.05), while Qmax increased from (5.6 ± 2.1) ml/s to (17.6 ± 2.7) ml/s (P < 0.05); the former three parameters in the αlA-b + ET group decreased from (133.6 ± 24.9) ml, (21.5 ± 3.2), and (4.7 ± 1.1) to (42 ± 18.3) ml, (12.8 ± 2.6), and (2.5 ± 0.7) respectively (P < 0.05), while the latter one increased from (6.3 ± 2.4) ml/s to (11.7 ± 2.3) ml/s (P < 0.05), all with statistically significant differences between the two groups (P < 0.05). The overall survival rate of the TURP + ET group was not significantly different from that of the αlA-b + ET group (51.4% vs 46.4% , P > 0.05).

CONCLUSIONTURP + ET is preferable to αlA-b + ET for its advantage of relieving BOO symptoms in advanced PCa without affecting the overall survival rate of the patients.

Adrenergic alpha-1 Receptor Antagonists ; therapeutic use ; Antineoplastic Agents, Hormonal ; therapeutic use ; Combined Modality Therapy ; methods ; Humans ; Male ; Prostatic Neoplasms ; complications ; drug therapy ; pathology ; surgery ; Quality of Life ; Retrospective Studies ; Transurethral Resection of Prostate ; Treatment Outcome ; Urinary Bladder Neck Obstruction ; drug therapy ; etiology ; surgery

Objective To investigate the protective effects of active components of notoginseng on immunological liver injury in mice. Methods Sixty Kunming mice were randomly divided into normal group (group A), model group (Group B), PNS group (Group C), total flavonoids group (Group D),and polysaccharide group (group E). C,D and E groups were given PNS, total flavonoids and polysaccharide orally, 1/day for 14 days. Then BCG was intravenous injected in B, C, D and E groups, and after 26 days lipopolysaccharide (LPS) was intravenous injected. Samples of eye venous plexus blood were collected, and serum levels of alanine aminotransferase (ALT), total superoxide dismutase (T-SOD) and Interleukin-4 (IL-4) were detected. Organs index was calculated by checking pathological results of liver, spleen and thymus. Results Com-pared with group B, the thymus index and serum ALT levels were significantly decreased in C, D and E groups (P<0.01), but the levels of IL-4 and T-SOD increased significantly (P<0.01). Pathological results showed that there were more serious inflammatory cell infiltration in liver, edema and necrosis of dot in C, D and E groups than those in group B. Conclusion The active components of notoginseng showed a significant protective effect on immunological liver injury.

Objective To investigate the sensitivity of dihydroartemisinin against different developmental stages of Schistosoma japonicum, so as to understand the effect of dihydroarteminisin against on S. japonicum. Methods Mice were infected with 40 S. japonicum cercariae on the abdomen. Dihydroartemisinin was given intragastrically at different developmental stages of S. japonicum , and the mice were sacrificed 50 days post-infection, the adult worms were collected, and the worm reduction rates and female worm reduction rates were calculated. ① On the 2nd h, 3rd, 5th, 7th, 10th, 14th, 18th, 21st, 28th, 35th day post-infection, the mice were administered intragastrically with dihydroartemisinin at a single dose of 300 mg/kg, and the effect of dihydroarteminisin on different developmental stages of S. japonicum were observed. ② The mice were administered with different doses of dihydroartemisinin on the 7th or 35th day post-infection, and the dose-effect was explored. ③ The mice were administered on the 7th day post-infection and re-administered on the 35th day post-infection, respectively with different doses of dihydroartemisinin, and the effect was evaluated. Results The dihydroartemisinin at a single dose of 300 mg/kg had obvious effect on 7-day schistosomula and 35-day adult worms, with 64.81% and 60.47% of worm reduction rates and 73. 81% and 90.48% of female worm reduction rates, respectively. When the mice on the 7th day post-infection were administered with 200, 300, 400 and 600 mg/kg dihydroartemisinin, the worm reduction rates were 46.84% , 60.63% , 59.55% and 60. 21% , respectively, and the female reduction rates were 59.73% , 72.29% , 72.58% and 76.61 % , respectively. While in the mice on the 35th day post-infection, the corresponding rates were 47. 23% , 62.33% , 76.31% and 83.63% , respectively, and 59. 73% , 89. 36% , 89.65% and 93.96% , respectively. When the mice were treated twice with dihydroartemisinin on the 7th day and 35th day post-infection, the worm reduction rates were 58. 16% , 82.66% ,83.42% and 83.79% , respectively, and the female worm reduction rates were 68.69% , 90.43% , 93.74% and 94.63% , respectively. Conclusions Dihydroartemisinin has effect against S. japonicum, and the 7-day schistosomulum and 35-day adult worm are sensitive to the drug.