Objective To investigate the value of DNA image cytometry in thediagnose of benign and malignant pleuroperitoneal fluids by comparing with the liquid-based cytological results. Methods There were 417 cases in-volved in this study. Pap stain for cytology analysis and feulgen stain for DNA image cytometry were used to identify benign and malignant pleuroperitoneal fluids respectively,then compared the results of the two methods. Results Clinically or pathologically,213 were classified as malignant and the other 204 as benign. The sensitivity, specifici-ty,accuracy, positive predictive value and negative predictivevalue of DNA image cytometry were 89. 7%,100%, 94. 7%,100%,and 90. 3%,respectively. However,the sensitivity,specificity,accuracy, positive predictive value and negative predictive value of liquid-based cytology were 63. 4%, 81. 9%, 72. 4%, 78. 5%, and 68. 2%, re-spectively. There were significant differences in thesensitivity, specificity,accuracy, positive predictive value and negative predictivevalue. Conclusion DNA image cytometry has great application value in the diagnosis of benign and malignant pleuroperitoneal fluids, and can increase the positive rate, reduce misdiagnosed rate with liquid-based cytology.

The treatment of chronic pain needs multidisciplinary management.Neurosurgical procedures hold an important role.With advancement in stereotactics,microsurgical and electrophysiological techniques,neurosurgical treatment becomes microinvasive,safer and popular.Neurosurgical procedures include anatomic,neuroaugmentative and neuroablative therapies.The procedure should be chosen individually.The pain characters,primary disorders,quality of life and survival time of the patient should be considered.A successful management must be well-indicated.

Antineoplastic Agents ; therapeutic use ; Chemotherapy, Adjuvant ; methods ; China ; Glioblastoma ; drug therapy ; surgery ; Humans ; Neurosurgery ; Physicians

Erythropoietin ; pharmacology ; Folic Acid ; pharmacology ; therapeutic use ; Humans ; Infant, Newborn ; Infant, Premature ; Platelet Count ; Vitamin B 12 ; pharmacology ; therapeutic use

OBJECTIVE: To provide reference for rapid and effective first aid to mine disaster before hospitalization. METHODS: The status quo of emergent rescue for mine disaster in China was reviewed. The key to rescue, main countermeasures and necessary drug of first aid before hospitalization were analyzed according to the characteristics of mine disaster and troubles of medical rescue. RESULTS & CONCLUSIONS: Ambulance corps cooperated with medical staffs to set ICU at disaster site. Scientific rescue provided by professional team can improve survival rate. Meanwhile, a perfect mine disaster rescue system should be set up.

Adult ; Female ; Humans ; Male ; Middle Aged ; Nasal Cavity ; surgery ; Nasal Septum ; pathology ; Nose Diseases ; surgery ; Reconstructive Surgical Procedures ; methods ; Surgical Flaps ; Tissue Transplantation ; methods

0bjective To determine the effect of 2 therapies(early realignment versus delayed open urethroplasty)for posterior urethral injuries associated with pelvic fractures. Methods A sys-tematic review of all the relevant clinical trials was performed.Trials were identified from specialized trials register of Cochrane UGDP Group,the Coehrane library,additional electronic search(mainly MEDLINE、EMBSAE、CBM)and manual searching.Clinical trials comparing early realignment and delayed open urethroplasty of posterior urethral disruption associated with pelvic fractures were includ-ed.Statistics analysis was managed by using Review Manager 4.2. Results Ten clinical trials inclu-ding 470 patients were included.Meta-analysis indicated that odds ratio(OR)of the rate of urethral stricture was 0.27[95%confidence interval(95%CI)0.08,0.86,P=0.03].The OR of the percent-age of strictures needing urethroplasty was 0.25,95%CI 0.08,0.74,P=0.01. Conclusions Early realignment may provide better outcomes than delayed open urethroplasty after posterior urethral dis-ruption.It can decrease urethral stricture formation and the percentage of strictures needing urethro-plasty.But the results might be influenced by the bias of grouping,especially the severity of injuries.

Objective To explore the effect of Dexamethasone(DEX)on the proliferation and the expression of fibroblast growth factor(FGF)- 1,FGF - 2 and interleukin - 6(IL - 6)mRNA in hyperoxia - exposed human em-bryo lung fibroblasts(MRC - 5). Methods High oxygen volume fraction of 950 mL/ L was used to establish hyperoxia -damaged cell models,then treated with different concentrations of DEX(10 - 4 ,10 - 6 ,10 - 8 ,10 - 9 ,10 - 10 ,10 - 11 ,10 - 12 mol/ L),respectively. The proliferation activity of the cells was evaluated by methyl thiazolyl tetrazolium(MTT)at 24 h,48 h,72 h,and the optimal hyperoxia - exposed time and concentration of DEX were chosen;then they were divided into air group,hyperoxia group and hyperoxia + DEX group. The mRNA expressions of FGF - 1,FGF - 2 and IL - 6 were detected by quantitative real - time PCR at 48 h. Results Cell proliferation activity of the hyperoxia group was lower than that of the air group and there were significant differences at 48 h and 72 h(all P ﹤ 0. 05). Compared with the hyperoxia group,cell proliferation activity of the hyperoxia + DEX group increased with the concentration of DEX decreasing,reaching the peak at 10 - 9 mol/ L,and then gradually decreased with the concentration of DEX decreasing. Cells were cultured for 48 h,compared with the air group,the level of FGF - 1 mRNA was lower,FGF - 2 and IL - 6 mRNA were higher in the hyperoxia group(P ﹤ 0. 05). Compared with hyperoxia group,the level of FGF - 1 mRNA was higher,FGF - 2 and IL - 6 mRNA were lower in hyperoxia + DEX group(P ﹤ 0. 05). Conclusions Exposure to high oxygen volume fraction of 950 mL/ L for 48 h can cultivate optimal hyperoxia - damaged cell models,and DEX can protect the cell from hyperoxia injury and 10 - 9 mol/ L was the optimal concentration. Enhanced the expression of FGF - 1 mRNA and inhibited expression of FGF - 2 mRNA may be one of the mechanism that DEX protects the cells from hyperoxia injury.