Selective COX-2 inhibitors can promote the apoptosis of tumor cells and block the growth of new blood vessels through the inhibition of ring oxidase,vascular endothelial growth factor,microvessel and so on. Research shows that COX-2 is overexpressed in vascular and endothelial cells of lung cancer,select COX-2 inhibitors to adjuvant chemotherapy,not only can directly inhibit the tumor proliferation,but also can reduce the adverse reaction of chemotherapy drugs,and enhance the curative effect. To investigate the effects of selec-tive COX-2 inhibitors and cisplatin in lung cancer angiogenesis and its mechanism,will be a hope for the clini-cal treatment of tumor.

Objective To investigate the clinical value of vaginal myomectomy. Methods From March 2005 to May 2008,78 women with abnormal uterine bleeding were diagnosed with submucous myoma by hysteroscopy combined with ultrasonography. All the myomas were located in the lower uterine segment,and the diameter of the tumor ranged from 30 mm to 63 mm. The patients were divided into two groups to underwent vaginal myomectomy (vaginal group,36 cases) or abdominal electrotomy (open group,42 cases). The operation time,intraoperative blood loss,postoperative hospital stay and recovery of the two groups were compared. Results The vaginal operation was accomplished in all of the 36 cases. Compared with the open group,the vaginal group had significantly shorter operation time [(64.7?10.4) min vs (71.1?11.3) min,t=-2.599,P=0.011],less intraoperative blood loss [(200.0?38.4) ml vs (253.6?47.6) ml,t=-5.412,P=0.000],quicker recovery of bowel movement [(18.5?4.3) h vs (30.9?4.7) h,t=-12.078,P=0.000],and shorter postoperative hospital stay [(4.9?1.2) d vs (7.0?1.3) d,t=-7.368,P=0.000]. Follow-up was available in all of the 78 cases for 3 to 40 months (mean,28 months),during which no relapse occurred,and no statistical differences in pregnancy rate between the two groups was revealed [2.8% (1/36) vs 4.8% (2/42),?2=0.000,P=1.000]. The operation satisfaction rates in both of the two groups were up to 100%. Conclusions Vaginal myomectomy has advantages in less hemorrhage,fast recovery,short postoperative hospital stay,and low complication rate. For the tumors located in the lower uterine segment or those larger than 30 mm in diameter,vaginal myomectomy is an alternative to hysteroscopic electrotomy.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Earthquakes ; Female ; Humans ; Male ; Middle Aged ; Pain Management ; Young Adult

Objective To explore the risk factors contributing to wound complications following operative fixation for fractured calcanea.Methods Fitly-six patients with 61 fractured calcanea who had been treated with operative fixation from March 2000 to March 2003 were enrolled in investigation.General information of the pa- tients,ages,injury causes,classification of fracture,fall heights,smoking,delayed operation time,operation time and tourniquet time were documented preoperatively and postoperatively to be analyzed statistically.Results We report an overall wound complication rate of 14.8%(9/61).Wound infection,haematoma,dehiscence,heel necrosis and osteomyelitis were noted in our series.We identified the following as risk factors of postoperative wound compli- cations:1)fall from more than 3.4 m high(P＜0.05);2)delayed operation time less than seven days(P＜0.05); 3)operating time beyond two hours(P＜0.05);4)tourniquet time beyond 1.5 hours(P＜0.05).Conclusion In order to decrease wound complications following operative fixation tor fractured calcanea,surgeons should pertorm the operation 7 to 10 days after the injury,and reduce the operation time and tourniquet time.

Objective:To investigate the retinal toxicity of chitosan and gentamicin- chitosan gel. Methods:Sixteen albino New Zealand rabbits (weighted 1. 5-2.5 kg) were used in this study. The rabbits were divided into 4 groups randomly;each group was injected with 0.1 ml PBS, gentamicin 2 mg/ml,3% Chitosan gel, and gentamicin-chitosan gel (2 mg/ml) respectively. ①Ophtahlmoscopic examinations were done. Retina was observed prior to injection , and on 1st, 3 rd, 7 th, 14 th d and 28 th d after injection with direct ophthalmolscope observations. ②Electroretinographs (ERGs) were recorded prior to injection, and then on 3 rd,7 th,14 th d and 28 th d after injection were carried out. ③Light microscope and transmission electronicmicroscope examinations were performed four weeks after the injection. All eyes were enucleated, the retina examples were observed under light microscope and transmission electronic microscope. Results:Intravitreous injection with 3% chitosan was free of retina toxicity. The retina toxicity effects of gentamicin 200 ?g could be found with transmission electron microscope, but gentamicin-chitosan gel with the same dosage could decrease the damage. Conclusion:The result shows that chitosan can be used in the intravitreous injection.

Animals ; Biogenic Monoamines ; metabolism ; Brain ; drug effects ; metabolism ; Female ; Lipid Peroxidation ; drug effects ; Male ; Mice ; Paraquat ; toxicity

Objective To investigate the multiple factors correlated to femoral head necrosis in patients over 45 years old with femoral neck fractures after closed reduction and cannulated screws fixation.Methods Three hundred patients with femoral neck fracture were admitted from May 1999 to May 2004 and treated by closed reduction and internal fixation with cannulated screws.We analyzed the multiple factors:age,gender,Garden classification,follow-up time,procedure delay,quality of reduction,time of full-weight-loading,fixation removal, and preoperative traction.Multiple correlation factors were analyzed by SPSS10.0 statistic system.Results Complete case records were documented in 99 cases who were followed up for an average of 24.5 months(range,8 to 60 months).Femoral head necrosis occurred in 15 cases(15.2%).Time of femoral head necrosis was 8 to 50 months after operation.The combination of quality of reduction,preoperative traction,age,and age?fixation re- moval was found to have the most significant influence on femoral head necrosis after internal fixation for femoral neck fractures.Conclusions Good reduction is the most important factor correlated to the outcome of a femoral neck fracture.Age is linked to the risk of necrosis but it is relatively insignificant.Preoperative traction in wrong positions can increase the incidence of necrosis.Garden classification is important in predicting femoral head necrosis and is associated with quality of reduction,but it is not a risk one in the combination of multiple correlation factors that lead to the necrosis.

ObjectiveTo observe the effection of different interventions of group B streptococcus (GBS) infection in gestation period.MethodsOne hundred and seventeen cases with GBS infection were obtained from 1885 pregnant women,who were got routine prenatal examination at 34 to 37 weeks of pregnancy,and drug seeitivity test of secretions which were taken from under paragraph 1/3 of vagina,and divided into treatment group (91 cases) and untreatment group (26 cases).The treatment group was divided into treatment group one (47 cases) and treatment group two (44 cases).Treatment group one was treated with oral antibiotics for 7 days after diagnosis,treatment group one and two were treated with postpartum antibiotics intravenous infusion once every 4 hours in labor.Comparison of maternal and fetal outcomes.ResultsThe GBS infection rate was 6.2％ (117/1885).The morbidity of premature delivery,premature rupture of membrane and neonatal infection of treatment group [ 5.5％ ( 5/91 ),13.2％ ( 12/91 ),5.5％ (5/91 ) ]were lower than those of untreatment group [ 19.2％(5/26),30.8％(8/26),23.1％(6/26) ](P ＜ 0.05).The morbidity of premature dehvery and premature rupture of membrane of treatment group one[ 0,6.4％ (3/47)]were lower than those of treatment group two[ 11.4％ (5/44),20.5％ (9/44)](P ＜ 0.05).Conclusion Anti-GBS treatment can improve the outcomes of mothers and infants,especially early anti-GBS treatment during the period of pregnancy.

Objective:To prepare anti-AchR monoclonal antibodies from rat bone marrow.Methods:The extracellular region of human AchR ?1 subunit was expressed in E.coli and purified using a Ni-NTA column.Then the protein was refolded by dialyzing.Lewis rats were immunized with purified AchR ?1 subunit and B cells from bone marrow and spleen were fused with myeloma cells to generate monoclonal antibodies.Results:Human AchR ?1 subunit was successfully expressed in E.coli.Rats immunized with the refolded protein were shown symptoms of Myasthenia gravis.Hybridoma cells were produced using B cells from rat bone marrow and spleen.Conclusion:Bone marrow cells can be immortalized using hybridoma method,which can be used for further study of Myasthenia gravis.