2.Comparative study of serum and synovial fluid monokine levels in patients with juvenile spondyloarthropathies and their clinical significance
Wei ZHAO ; Feng HUANG ; Ming XU
Chinese Journal of Rheumatology 2000;0(06):-
Objective To determine the monokine levels of serum and synovial fluid (SF) in patients with JSpAs and estimate the correlation between monokines and inflammatory indices.Methods Serum and SF monokines (IL 1?,IL 1?,IL 6,IL 8,IL 12 and TNF ?) were measured by sandwich ELISA in patients with JSpAs ( n =28) and healthy controls ( n =10).The correlation between serum monokines and inflammatory indeces and between both serum and SF monokine levels was analysed.Results Elevated serum level of IL 6 was found in patients with JSpAs as compared to healthy controls ( P
3.Relationship between CK18 expression in pathologically negative lymph nodes and Tiam 1 mRNA expression in the gastric cancer tissue.
Wei XU ; Hong-Li SHAN ; Ming-Wei ZHANG
Chinese Journal of Oncology 2009;31(12):919-920
Adult
;
Aged
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Aged, 80 and over
;
Female
;
Guanine Nucleotide Exchange Factors
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genetics
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metabolism
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Humans
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Keratin-18
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genetics
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metabolism
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Lymph Nodes
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metabolism
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pathology
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Lymphatic Metastasis
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Male
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Middle Aged
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RNA, Messenger
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metabolism
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Stomach Neoplasms
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metabolism
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pathology
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T-Lymphoma Invasion and Metastasis-inducing Protein 1
6.Neoplasty of multiple cerebrospinal fluid rhinorrhea on combined frontal-nose approach through endoscope.
Wei-Yuan SUN ; Fu-Ming ZHU ; Xin-Hua XU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(6):460-462
Cerebrospinal Fluid Rhinorrhea
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surgery
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Craniotomy
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methods
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Endoscopy
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Frontal Sinus
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surgery
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Humans
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Male
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Nose
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surgery
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Reconstructive Surgical Procedures
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methods
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Young Adult
7.Study on expression of stem cell marker SALL4 gene in cervical cancer
Ming ZHANG ; Yiming ZHANG ; Wei ZUO ; Hui QIAN ; Wenrong XU
Chongqing Medicine 2014;(3):285-287
Objective To research the expression and clinical significance of SALL 4 gene in cervical cancer .Methods The ex-pression of SALL4 in 56 samples of cervical cancer and 35 samples of normal cervical tissues was detected by immunohistochemistry and RT-PCR ,and its relationship with the clinicopathological characteristics of cervical cancer was analyzed .Results The expres-sion of SALL4 mRNA was 2 .56 ± 0 .22 in cervical cancer tissues ,which was significantly higher than 0 .38 ± 0 .03 in the normal cer-vical tissues .the difference between them had statistical significance(t=58 .1 ,P<0 .01);the positive expression rate of SALL4 pro-tein was 80 .4% (45/56) in cervical cancer ,which was significantly higher than 11 .4% (4/35) in the normal cervical tissues (χ2 =41 .177 ,P<0 .01) .The positive expression of SALL4 in the cervical cancer tissues was correlated with the differentiation status of tumor ,which in the middle and high differentiation groups was lower than that in the low differentiation group (χ2 =4 .226 ,P=0 .039) ,but had no correlation with age ,International Federation of Gynecology and Obstetrics (FIGO) stage ,tumor size ,pathologi-cal type and lymph node metastasis(P>0 .05) .Conclusion SALL4 is highly expressed in the cervical cancer tissues and correlated with the tumor differentiation ,which might play an important role in the occurrence and development of cervical cancer .
8.Expression of a Kind of Curcin Induced from Jatropha curcas (curicin 2) in Tobacco
Ming-Xing HUANG ; Qin WEI ; Ying XU ; Fang CHEN ;
China Biotechnology 2006;0(04):-
A new kind of curcin (curcin 2), induced by several kinds of stresses from Jatropha curcas leaves, under the control of the 35S CaMV (cauliflower mosaic virus) promoter, was introduced into tobacco genome by Agrobacterium tumefaciens-mediated transformation method. Curcin 2 protein was only detected in the transgenic tobacco plants transformed with the cur2p fragment (coding premature curcin 2 protein), but not in the plants transformed with cur2m fragment (coding mature curcin 2 protein). The transgenic lines expressing curcin 2 showed increased tolerance to tobacco mosaic virus (TMV).
9.New conception about the processing of Indigo Naturals
Qi WEI ; Ming YANG ; Runchun XU ; Qiling SUN ; Yunjiang HU ;
Chinese Traditional Patent Medicine 1992;0(02):-
AIM: To study the processing of Indigo Naturals. METHODS: Study on the microstructure of Indigo Naturals with scanning electron microscope (SEM) and X ray diffractometer (XRD) were carried out. RESULTS: Through the SEM, it is clear that Indigo attached to the surface of some white substance, and the XRD proves that the white substance in Indigo Naturals is calcium carbonate (CaCO 3). CONCLUSION: The study about the processing of Indigo Naturals indicates that Indigo Naturals consists of Indigo and other effective compositions attached the surface of calcium carbonate (CaCO 3).
10.Identification of Viticis Fructus and Its Adulterants by ITS2 Sequence
Xiaocun ZHANG ; Di XU ; Wei SUN ; Ming SONG ; Xia LIU
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2366-2370
ITS2 sequence was used as a barcode to identify Viticis Fructus and its adulterants. The study collected 46 samples include Viticis Fructus and its adulterants. The sequences were obtained by extracting DNA, amplification, sequencing bi-direstionlly, and then assembling with CodonCode Aligner. To identify Viticis Fructus and its adulterants, the study used the methods of computing the genetic distances by Kimura 2-Parameter (K2P) model and constructing the Neighbor-joining (NJ) phylogenetic trees using MEGA5.0. The results showed that the maximum inter-specific K2P distance of Viticis Fructus was less than the minimum inter-specific K2P distance of Viticis Fructus and its adulterants and the NJ tree indicated that the Viticis Fructus and its adulterants could be distinguished clearly. Therefore, using ITS2 barcode can distinguished Viticis Fructus and its adulterants accurately.