1.Clinical application of gemcitabine in chemotherapy of cervical cancer
Hong PEI ; Min WEI ; Baoqin ZHOU
Journal of Medical Postgraduates 2003;0(11):-
Objective: To investigate the efficacy of gemcitabine in chemotherapy of cervical carcinoma. Methods:10 untreated cervical carcinoma patients had been divided into two groups,5 cases were treated with neoadjuvant chemotherapy and radiotherapy,others were treated only with radiotherapy. All patients were operated and compared the pathologic changes of cancers. Moreover 3 cases with recurrent tumors had been treated with gemcitabine and other agents . Results: The rate of complete response(CR) was 60% in group of neoadjuvant chemotherapy and 20% in patients with only radiotherapy. Only one case achieved partial response (PR) in patients with recurrence. Conclusion: The neoadjuvant chemotherapy in cervical carcinoma with gemcitabine is helpful for achieving good response .As radiation sensitizer or radio-potentiator,gemcitabine can promote efficacy of neoadjuvant chemotherapy in cervical carcinoma.
2.Treatment of ovarian granulose cell tumor of 24 cases
Hong PEI ; Baoqin ZHOU ; Min WEI
Journal of Medical Postgraduates 2004;0(01):-
Objective: To study the factors relating the prognosis of ovarian granulosa cell tumor and the characteristics of its clinical presentations. Methods: The clinical records and pathological sections of 24 cases with granulosa cell tumor of ovary were reviewed retrospectively. The clinical characteristics, the clinical stage, and the therapeutic programs and the results were summarized respectively. Results:The 5-years survival rate showed that the clinical stage influenced the prognosis of ovarian granulosa cell tumor obviously. Conclusion: The early dection is the major factor which influences the patients' survival rate.
3.Application effect of Fufukangspray in post partum women
Chongqing Medicine 2015;(8):1080-1081
Objective To explore the application effect of Fufukangspray in postpartum women in the treatment of vaginitis . Methods From July 2013 to June 2012 in our hospital ,80 cases of postpartum vaginitis patients as the research object .The control group was treated with Metronidazole Suppositories treatment ,the experimental group were treated with Fufukangspray treatment . Comparison of the two groups in the therapeutic effect and adverse reaction of differences .Results Compared with the control group ,we found that the experimental group total effective rate was significantly higher ,the incidence of adverse reaction was low‐er ,the differences between groups by chi square test analysis that was statistically significant(P<0 .05) .Conclusion Fufukang‐spray has good application effect in the treatment of postpartum vaginitis ,curative effect ,less adverse reactions ,it is worthy of clini‐cal application .
4.Influence of surgical resection extent on prognosis of high-risk gastrointestinal stromal tumors
Chongqing Medicine 2014;(25):3290-3292
Objective To investigate the clinical characteristics,surgical resection extent and prognosis influencing factors of pri-mary high-risk gastrointestinal stromal tumors(GIST).Methods The clinicopathological and follow-up data of 48 patients with pri-mary resectable high-risk GIST in this hospital from January 2005 to January 2013 were retrospectively analyzed.The influence of three kinds of different surgical resection methods on the prognosis of GIST was analyzed.Results 34 cases were treated by R0 re-section(70.83%),6 cases by R1 resection(11.50%)and 8 cases by R2 resection(16.67%).All cases were given the postoperative adjuvant therapy of imatinib mesylate.The average medication time was(26.5±13.6)months.The occurrence rate of adverse drug reactions was 100%,and the main symptoms were swelling and leukopenia.The 5-year survival rates in 3 kinds of resection modes were 97.06%,66.67% and 25.00% respectively.The univariate and multivariate prognostic analysis showed that the surgical re-section extent and the tumor rupture were the independent prognostic factors in the high-risk GIST patients.Conclusion The intact operative resection and preventing the tumor rupture are the keys to increase the postoperative survival rate of GIST patients.
6.Alterations of p14~(ARF) and p53 genes in human primary colorectal carcinomas
Shaohui TANG ; Donghua YANG ; Wei HUANG ; Min ZHOU ; Hongke ZHOU
Chinese Journal of Pathophysiology 1989;0(06):-
AIM: To investigate the genetic and epigenetic alterations of p14~ ARF gene and mutation status of p53 gene in human primary colorectal carcinomas and to analyze the relationship between the two gene changes and the role of abrogation of the p14~ ARF -p53 pathway in colorectal carcinogenesis. METHODS: The homozygous deletions, mutations, methylation of 5′ CpG islands, mRNA expression of p14~ ARF gene and mutations of p53 gene were assessed by PCR, direct sequencing, methylation-specific PCR, and RT-PCR in the tumorous and matched adjacent normal colorectal tissues from 56 patients with colorectal carcinoma. RESULTS: ① p14~ ARF alterations were detected in 27% (15/56) of colorectal carcinoma tissues studied, of which 1 case showed homozygous deletion, 14 cases showed 5′ CpG island methylation, and no mutation was found in any tumor. ②15 colorectal carcinomas with p14~ ARF alterations indicated lack of (13 cases) or at low level of expression (2 cases) of p14~ ARF mRNA, while expression of the p14~ ARF transcript was detected in the remaining 41 colorectal carcinomas and any matched adjacent normal colorectal tissues. ③ The mutations of p53 gene were detected in 48% (27/56) of colorectal carcinomas investigated. ④ Of these 56 cases, 12 had p14~ ARF alterations alone, 24 had p53 mutations alone, 3 had both p53 mutations and p14~ ARF methylation, and 17 had neither. 70% (39/56) of the samples had either or both abnormalities of the two genes, and p14~ ARF hypermethylation was related to wildtype p53 (P
7.EFFECT OF PSYCHOLOGICAL STRESS ON THE CONCENTRATION OF SERUM IRON IN RATS AND ITS MECHANISM
Jian ZHOU ; Min LI ; Chunlan WEI ; Min ZHAO ; Jianbo CHEN
Acta Nutrimenta Sinica 1956;0(03):-
Objective To investigate the effect of psychological stress on serum iron concentration of SD rats and its mechanism. Method SD rats were randomly divided into four groups: control group (CG), psychological stress 1w group (PG 1w) and 2w group (PG-2w), and psychological stress 2w with 1w free group (PG 2w-1w). The animal model of stress was tested for 1w, 2w, and 2w with 1w free from stress. The iron concentration of serum, liver, spleen and the expression of liver hepcidin were detected by atomic absorption spectrometry and Western blot respectively. Result (1) Compared with control group, serum iron concentration of rats under stress decreased. (2) Free of psychological stress 1w later, serum iron concentration was not significantly changed. (3) Iron concentration of liver and spleen was increased significantly compared with control group, and the expression of liver hepcidin was also increased. Conclusion Psychological stress may cause serum iron of rats to decrease obviously, and it was restored after 1w free from psychological stress. The mechanism is supposed to be increase of iron storage in liver and spleen by hepcidin in rats under psychological stress.
8.Delayed Brain Myelination in Children with Phenylketonuria Complicated with Epilepsia
zhi-xin, WANG ; zhong-shu, ZHOU ; wei-min, YU
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To observe the delayed brain myelination of children with phenylketonuria(PKU)combined with epilepsia,and explore effectiveness of the treatment and provide an objective criteria for patient recovering evaluation.Methods There were 42 PKU patients,aged 3 to 72 months were selected.The concentration of phenylalanine tested by high pressure liquid chromatography was greater than 1.2 mmol/L in blood,diagnosed as PKU.According to electroencephalogram and clinical symptom,21 cases were diagnosed as epilepsy,the other 21 cases were used as control group.All patients were taken MRI before treatment.Myelination in 10 sections(cerebellum,pons,mesencephalon,internal capsule posterior limb,corpus callosum,internal capsule anterior limb,occipital lobe,parietal lobe,temporal lobe,frontal lobe)were evaluated.Results Delayed myelinations were located mainly in the cerebral lobes and corpus callosum,average delayed incidence of the 10 region was 44.8% in epilepsy group and 30.9% in control group.The incidence of the corpus callsum was 80.9% in epilepsy group,52.4% in control group,the number of sections of delayed myelination showed statistically significant between 2 groups(P
9.Expression, purification, and functional identification of immunoglobulin degrading enzyme IdeS in Escherichia coli
Si-han ZHOU ; Min-zhi LIU ; Yan YANG ; Wei WANG
Acta Pharmaceutica Sinica 2022;57(7):2234-2239
In the process of evolution, pathogenic