BACKGROUND: Reactive depression and depressive neurosis are both psychogenic emotional disorders, clinically manifested by memory deterioration,impaired concentration, slow reaction, and bradyphrenia, among other cognitive function impairments. These subjective experiences of such patients still need to be backed up for their actual presence by objective laboratory evidences, which,however, have been scarcely available to our current knowledge.OBJECTIVE: To investigate the changes of auditory event-related Potential (AERP) P300 in patients with reactive depression and depressive neurosis, and their correlation with cognitive function impairments.DESIGN: Case-controlled experiment.SETTING: Clinical Center of Psychological Counseling, Department of Neurology, Third Military Medical University of Chinese PLA. PARTICIPANTS: Forty normal control subjects were recruited from the staff of Southwest Hospital, Third Military Medical University between September 1997 and March 1998, including 21 male and 19 female subjects aged 20-50 years. Eighty patients were selected from the agematched outpatients of the same hospital seeking psychological counseling,including 40 with reactive depression (consisting of 29 male and 11 female patients) and 40 with depressive neurosis (consisting of 24 male and 16 female patients). From these patients 10 with abnormal P300 potential were randomly selected for followed-up study, including4 with reactive depression and4 with depressive neurosis.METHODS: All the patients and normal controls were subjected to conventional examination of AERP P300 in the Laboratory of Cerebral Electrophysiology, and psychological evaluation was carried out by specialists with the assistance of Zung Self-Rating Depression Scale.MAIN OUTCOME MEASURES: ① Results of AERP P300 examination and scores of Zung Self-Rating Depression Scale in the 80 patients upon admission and 4 and 8 weeks after treatment, and results of AERP P300 examination in the control group. ② Results of AERP P300 examination and scores of Zung Self-Rating Depression Scale in the follow-up group. RESULTS: All subjects finished the study. The latency of all waves were remarkably prolonged in the patients as compared to the normal control group (P ＜ 0.05-0.01), with also lower amplitude of AERP than that of the control group; the amplitudes of N1, P2 and N2 waves, in general,tended to decrease to statistically significant levels. In reactive depression group, the amplitude of P3 wave was obviously lower than that of the control group [(10.39±4.40) vs (15.11±4.16) μV, P ＜ 0.001], and the amplitudes of all waves in the neurosis group was obviously lower than those of the control group. Comparison of the latency and amplitude of P3 in the follow-up group before and after treatment revealed no significant difference in the latency of P3 [(314.10±44.08) vs (295.4±20.74) ms, t=1.21, P ＜ 0.05], but the amplitude of P3 obviously increased after treatment to almost the normal level [(6.38±2.50) vs (14.22±2.51) μV, t=-7.012, P ＜ 0.001].CONCLUSION: Patients with reactive depression or depressive neurosis have cognitive function impairment, and auditory event-related potential P300 can be used as a reference for cognitive evaluation and therapeutic effect assessment in these patients.

Objective To reconfirm the mesangial cell differentiation potential of BMSCs and to study the mechanism of cell induction.Methods Rat BMSCs were isolated and incubated with platelet-derived growth factor(PDGF)-BB to induce the mesangial cell phenotype.At day 7,the cell morphology,mesangial cell-specific markers and cell contraction function were examined.MAPK/ERK kinase inhibitor(PD98059) was added to examine its impact on cell growth and mesangial cells marker expression.Results After cultivation under the differentiation condition for 7 days,the induced cells expressed ?-actin and Desmin,which highly resembled cultured mesangial cells in rat.The induced cells with increasing level of PDGF receptors mRNA expression changed into a wide range of shapes from spindle to stellate and were contracted in response to angiotensin Ⅱ.PD98059 perturbed the induced cells mesangial cell-specific markers mRNA expression.Conclusion BMSCs can differentiate into mesangial-like cells with PDGF-BB induction in vitro.The mechanism involved in the differentiation was mediated through MAPK/ERK1/2 signal transduction pathway.

Diagnostic Errors ; Female ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell ; diagnosis ; Lymphoma, Mantle-Cell ; diagnosis ; Middle Aged

Objective To investigate whether IL-17 could stimulate the vascular endothelial growth factor (VEGF) production on HaCaT cells alone. We also investigated whether shikonin could inhibited the proinflamation effects of interleukin-17(IL-17) acting on HaCaT cells. MethodsWe examined the expression of VEGF by double antibody sandwich enzyme-linked immunosorbent assay ( ELISA ) and realtime polymerase chain reaction(RT-PCR) in HaCaT cells and the cell supernatant. The viability of HaCaT cells in the drug group was detected by the Cell Counting Kit-8 (CCK-8). ResultsThe expression of VEGF in different time IL-17-stimulated groups on HaCaT cells and the cell supernatant were higher than the control group( P＜0.001 ). The expression of VEGF in different drug treatment groups on HaCaT cells and the cell supematant were lower than the stimulated group by IL-17 ( P＜0. 001 ). The cell viability of different drug treatment groups have no significant difference( P＞0.05 ). ConclusionWe show that IL-17 specifically and time-dependently augmented and induced VEGF expression on HaCaT cells and the cell supernatantThen shikonin markedly inhibited the increase tengency of IL-17 effection on HaCaT cells and the cell supematant level.

Objective To compare the effect of set-up errors to two different body positions that are putting arms on the side of the body (A group) or placing arms folded across the elbow on forehead (B group) in esophageal cancer' fraction radiotherapy.Methods By using case-control study,all supine patients were divided into the A group and the B group.After patients were fixed by thermoplastic membrane,they were located by spiral CT and treated by using IGRT.During treatmwnt,there were three cone-beam CT scans in the first week and then at least one scan weekly.We obtained the linear set-up errors data by using bone registration with manual proofreading in 3 directions that were left and right (x),head and feet (y),abdomen and back (z) by using CBCT.Means were compared by using independent sample t-test,and ratios were compared by chi-square test.Results Every group had 11 cases that the A group had 92 people times and the B group had 87 people times.Absolute set-up errors of two different positions:only x-axis,the mean of the A group was (2.46 ± 1.79) mm.But the mean of the B group was (1.91 ± 1.71)mm,which was significantly less than the A group (P =0.036).Relative set-up errors of two groups:only y-axis,the mean of the B group was (1.91 ± 4.12) mm.The A group was (0.09 ± 3.90) mm,which was significantly less than the B group (P =0.003).There was no significant difference of three-dimensional set-up errors (P=0.751).And there was no significant difference in absolute weekly set-up errors or weekly three-dimensional set-up errors in radiotherapy (P ＞ 0.05).Conclusions The each of two body positions in esophageal cancer' fraction radiotherapy has advantage and disadvantage for set-up errors,but they were in control and didn't affect the radiotherapy planning.We can choose appropriate treatment position according to clinical practice.

Objective:To explore influence of nursing intervention according to comprehensive geriatric assessment (CGA)on quality of life (QOL)in aged patients With coronary heart disease (CHD).Methods:A total of 96 aged CHD inpatients admitted in department of cardiology from Mar 2012 to Mar 2013 Were enrolled.According to num-ber table method,they Were randomly divided into routine nursing group (n=48,received specific nursing of cardi-ology department)and CGA group (n=48,received nursing intervention according to CGA based on routine nurs-ing).Chinese questionnaire of quality of life in patients With cardiovascular disease (CQQC)Was used to score and compare each dimension of QOL betWeen tWo groups at admission and on 6-month after discharge.Results:Com-pared With routine nursing group after intervention,there Were significant increase in scores of physical strength [(7.35±4.12)scores vs.(10.86±5.06)scores],patient's condition [(4.57±0.85)scores vs. (8.75±4.54) scores],medical condition [(4.72±0.82)scores vs.(5.90±0.40)scores],general living [(3.85±1.45)scores vs. (5.22±1.95)scores],social psychological conditions [(3.67±1.52)scores vs.(8.41±3.22)scores],Working sta-tus [(0.70±0.64)scores vs.(0.97±0.66)scores]and total score of CQQC [(24.86±4.43)scores vs.(40.11± 9.05)scores]in CGA group,P<0.01 or<0.05.Conclusion:Nursing intervention according to comprehensive geri-atric assessment can improve quality of life in aged patients With coronary heart disease.

Objective To detect plasma progranulin (PGRN) level in subjects with newly diagnosed type 2 diabetes mellitus and to investigate the relationship of plasma PGRN level with glycolipid metabolism,inflammation,and insulin resistance.Methods Eighty patients with newly diagnosed type 2 diabetes (T2DM) and 88 subjects with normal glucose tolerance (NGT) were recruited in the study.Both of them were divided into normal weight (NW)subgroup and obesity (OB) subgroup.Obesity was defined as body mass index (BMI) ≥ 25 kg/m2 according to the World Health Organization-Western Pacific Region diagnostic criteria(2000).Body fat parameters were measured and BMI,waist-to-hip ratio were determined.Fasting plasma PGRN and interleukin-6 (IL-6) levels were determined by ELISA,fasting plasma glucose (FPG),2 h plasma glucose after glucose loading (2hPG),HbA1C,fasting insulin (FINS),and lipids were also detected.Insulin resistance and pancreas β cell function were assessed by homeostasis model assessment (HOMA-IR,HOMA-β).Results Plasma PGRN level was significantly higher in T2DM group than that in NGT group(P＜0.01).Within groups of T2DM and NGT,plasma PGRN level in OB subgroups was higher than that in NW subgroups [(225.22 ± 34.39 vs 195.59 ± 50.47 and 183.79 ± 61.63 vs 148.69 ± 55.27) ng/ml,P＜0.05].Bivariate correlation analysis showed that plasma PGRN level was positively correlated with weight,waist circumference,BMI,systolic blood pressure,FPG,2hPG,HbA1C,triglyceride(TG),IL-6,FINS,and HOMA-IR (P＜0.05 or P＜0.01),and was negatively correlated with HOMA-β (P＜0.05).Multiple linear regression analysis showed that BMI,HbA1C,IL-6,and TG were independently related to plasma PGRN level(P＜0.05).Conclusions Plasma PGRN level was increased in patients with type 2 diabetes as well as in obesity,and was closely related with glycolipid metabolism,inflammation,and insulin resistance.

To investigate the relationships among plasma secreted frizzled-related protein ( sfrp) 5 level and body fat parameters, glucolipid metabolism, insulin resistance index, and inflammation. 89 subjects with normal glucose tolerance(NGT) and 87 patients with type 2 diabetes mellitus (T2DM) were enrolled and each group was divided into no-obese and obese subgroups. Obesity was defined as body mass index ( BMI)≥25 kg/m2 according to the World Health Organization -Western Pacific Region diagnostic criteria ( 2000 ) . Body fat parameters were measured and BMI, waist-hip ratio were evaluated, meanwhile, the levels of blood glucose-lipid parameters and fasting insulin were also determined. Insulin resistance index ( IR) was assessed by homeostasis model assessment ( HOMA) . The concentrations of plasma sfrp5 and interleukin 6 were detected by enzyme-linked immunosorbent assay. Plasma sfrp5 level in T2DM group was significantly lower than that in NGT group [(8. 35±3. 38 vs 11. 35±3. 69)ng/ml, P<0. 01]. The levels of plasma sfrp5 in subjects with obesity were also lower than those in subjects with no-obesity in both NGT and T2DM groups [(9. 46±2. 70 vs 13. 12±3. 62)ng/ml and(6. 70±2. 34 vs 10. 12±3. 45) ng/ml, both P<0. 01]. Plasma concentrations of sfrp5 in T2DM-obese group were significantly lower than that in NGT-obese group(P<0. 01). Correlation analysis showed that plasma sfrp5 levels were negatively correlated with waist-hip ratio, HbA1C, fasting insulin, triglycerides, waist circumference, fasting plasma glucose, interleukin 6, natural logarithm of HOMA-IR [ln(HOMA-IR)], and BMI(P<0. 01 or P<0. 05). Multiple linear regression showed that ln(HOMA-IR), BMI, triglycerides were independent related factors in influencing the levels of plasma sfrp5 (r2=0. 216, 0. 177, 0. 113, all P<0. 05). Plasma sfrp5 levels were decreased in obesity and T2DM subjects and were correlated with body fat disposition, glucose-lipid metabolism, insulin resistance and inflammation. Lack of sfrp5 may contribute to the pathophysiology of obesity and T2DM.

Objective To explore the relationship between the women menopausal period and metabolic syndrome (MS) .Meth-ods The female residents of Chongqing urban areas above 40 years were selected as the investigation group ,all the subjects were performed the questionnaire survey and the physical examination ,at the same time the biochemical indexes were detected .Finally , 1402 women of natural menopause were included in this study .The study subjects were divided into different groups according to the menopausal period of <5 years ,5- <10 years ,10- <15 years and ≥15 years .The Logistic regression analysis was adopted to analyze the relationship between the menopausal period with MS and its components .Results The MS prevalence in this group was 40 .87% ,and the menopausal period of the women with MS was significantly higher than that without MS (P<0 .05) .The MS prevalences of postmenopausal women in the menopause period of <5 years ,5 - <10 years ,10 - <15 years and ≥15 years were 29 .37% ,34 .29% ,45 .30% and 49 .13% respectively (P<0 .05) .After adjustment for age and BMI (except central obesity ) ,the MS risk in the menopausal period of 10- <15 years and was 1 .54 times of that in the menopausal period of <5 years .The Logis-tic regression analysis showed that BMI and menopausal period were the influence factors of MS .Conclusion Post-menopausal women are the high-risk group of MS and the menopausal period is correlated to MS .

Objective To explore the effect of sequencing nursing on shortening cataract operation time and accelerating turnover of patients.Methods A record of operation time and postoperative vision for 630 cataract patients in Neijiang and Ya' an was studied.The effect of sequencing nursing and the operation quality and speed were evaluated.Results The average visual acuity of two groups was (0.31±0.082),(0.33±0.067)respectively (t =0.695 ,P =0.487 ＞0.05) ; in the experiment group,average time of operation was (17.0±1.1)min,control group was (27.2±2.9)min (t =18.924,P ＜0.01).Conclusions The sufficient preoperative preparation and sequencing nursing could reduce operative time,and increase the efficiency of cataract operation obviously.