Objective To explore the correlative factors of hypotension among hemodialysis patients and to come up with nursing interventions.Methods The histories of 49 maintenance hemodialysis patients were retrospectively reviewed to find out the correlative factors of hypotension and summarize the nursing strategies.Results The major factors for hypotension included large volume of dehydration and fast speed of dehydration in 22 cases,malnutrition and hypoproteinemia in 8 cases,severe anemia in 6 cases,dysfunction of the autonomic nervous system in 5 cases,low concentration of sodium or high temperature of dialysate in 4 case and medication errors and eating too much before or during dialysis in 4 cases.Conclusion The nursing strategies including control of weight,correct assessment of dry weight,right performance of low temperature hemodialysis and sodiun profile hemodialysis as well as mental care to those with depression are critical for prevention of hypotension development in patients undergoing maintenance hemodialysis.

Objective To understand the epidemiological characteristics of bacillary dysentery and etiological characteristics of Shigella in Tianjin, and to provide evidence for the prevention and control measures. Methods The data of disease surveillance information reporting system from 2008 to 2014 were analyzed retrospectively. A total of 3955 stool samples came from the clinically diagnosed cases of bacillary dysentery were collected, according to the extraction method of stratified sampling in Tianjin city, suburban area, Binhai New Area and suburban area sentinel hospitals, and according to the sampling interval for the May to October from 2008 to 2014. Samples were detected and cultured for Shigella flora. Results (1) Epidemiological characteristics:from 2008 to 2014, a total of 65179 cases of bacillary dysentery were reported in Tianjin city, with an average annual incidence of 72.00/100 thousand. The annual incidence rate showed a downward trend year by year. The incidence was a downward trend, including urban (120.28/100 thousand), suburban areas (70.36/100 thousand), Binhai New Area (64.22/100 thousand) and the outer suburbs (19.39/100 thousand). The onset time was mainly concentrated in June-September, and the peak incidence occurred in August. The incidence rate was higher in people below 5 years old and above the age of 85. The annual incidence rate was higher in male patients of 0 to 24 years old and above 75 years old than that of female. The annual incidence rate was higher in female patients of 50 to 74 years old age group than that of male (P<0.05). The distribution of occupation was scattered among children and retirees. (2) Distribution of bacteria:the 229 Shigella strains were positive (5.79%), including 136 strains of Shigella sonnei (59.39%), 93 strains of Shigella flexneri (40.61%). The 89 strains of Shigella flexneri (4 strains were not detected) were detected in 8 subtypes, F2a (49.44%), F2b (22.47%) and FX (13.48%). The subtypes of Shigella flexneri were detected decreased year by year from 2008 to 2014, showing a single subtype. The highest detection rate of Shigella was found in 10-19 years old (9.55%). The S.sonnei positive rate was the highest in urban (5.09%), and the Shigella flexneri positive rate was the highest in suburban areas (3.09%). Conclusion The key population for the prevention and control of bacillary dysentery in Tianjin is scattered children and old people. It is necessary to strengthen the propaganda and health education to improve the health consciousness of the key population and their daily nursing staff. With the continuous changes of Shigella flora, it is necessary to carry out long-term monitoring to find out the regularity of shigella.

Objective To explore the application of RNA interference(RNAi) in colorectal cancer gene therapy.Methods The related literatures in recent years were reviewed.Results RNAi causes a high effective and distinctive degradation of mRNA homologous in sequence to the dsRNA.This new technology has been successfully applied to research the genesis and the growth of colorectal cancer.Conclusion RNAi has been a new focus in gene therapy for colorectal cancer.

Objective To study the genetic polymorphisms of STR locus D20S85 and to obtain the genetic data of Guangdong Han, Guangxi Zhuang, Huadong Han,Neimenggu Han and Meng populations. Method Using PCR and silver - staining methods, the alleles of D20S85 locus were detected and analysed. Results 9 alleles and 35 genotypes were found in those populations. The highest allele frequency was D20S85* 6. The results of Hardy - Wein-berg equilibrium test showed that the genotype distributions observed in five populations were correspondent with the expected. The expected heterozygosity was 0.7720 - 0.7912; the exclusion probability of paternity was 0.7538-0.7594(triplet) and 0.3988 -0.4297 (biplet) ; the discrimination power 0.9175 -0.9272 and the polymorphism information content 0.7442 - 0.7656. Conclusion STR D20S85 locus is a useful genetic marker for forensic application .

Objective To investigate the expression of proliferating cell nuclear antigen (Ki67) in hepatitis B virus (HBV) related hepatocellular carcinoma (HCC) tissue and its correlation with clinicopathological parameters of HBV related HCC. Methods HBV-related HCC patients (n=134) were divided according to Ki67 index (the percentage of Ki67-posi?tive cells) into Ki67 index<10%group (negative group, n=30) and Ki67 index≥10%group(positive group, n=104). The cor?relation of Ki67 index with age, gender, glypican 3 (GPC3), histological grade, BCLC (Barcelona Clinic liver cancer) staging, portal involvement, AFP (AFP), liver function related child-pugh classification data were calculated in both groups. Ki 67 in?dex is set according to the interval score to assess the relationship between Ki67 score and BCLC classification. Results (1) SP staining showed, Ki67 present a trend of increase from normal liver tissue to well-differentiated to medium differentiat?ed tissue to poorly differentiated tissues intensity. (2) In positive group, Ki67 index was correlated with BCLC staging, portal metastasis, histological grade, GPC3 and AFP (P<0.05);By contrast, in negative group, Ki67 index was not correlated with any clinical parameter (P>0.05). (3) Ki67 score increases with the progress of BCLC classification. Conclusion In Ki67 positive group, Ki67 index combined with GPC3, histological grade, BCLC staging, portal involvement, AFP and liver func?tion child-pugh classification can assess the severity of the patients with HCC, which is expected to become an important in?dicator for the HBV-related HCC early diagnosis.

Objective To investigate the clinical effect of plastic surgery of double-palpebra with reposition of lacrimal gland for blepharochalasis.Methods We operated on 10 cases (20 eyes) with dermatolysis palpebrarum which stopped attack and was static for more than one year.A double eyelid fold incision was made.Then redundant skin and sphincter palpebrarum as well as protrusion of intraorbital fat were removed.In the operation of dislocation of lacrmial gland we sewed up lacrmial gland in full thickness and fixed it to the periosteum of lacrmial fossa,and the levator muscle of upper eye lid aponeurosis prothesis or plication was choosed when the levator muscle of upper eye lid aponeurosis was broken or not.And for the shorted transverse diameter of coloboma of eye lid we sewed up the external palpebral ligament and fixed it to the periosteum of lateral orbital margin.Results All the 10 patients got satisfactory therapeutic effect,the cutis laxa had been amended obviously,the good upper double eyelid shape could be achieved,the reposition of lacrimal gland was finished,lapsus palpebrae superioris and shorted transverse diameter of coloboma of eye lid had been rectified satisfactorily.All cases we followed up for three monthes to one year,and dislocation of lacrmial gland had been amended in one case,others had been reposited well,without recurrence.Conclusions Blepharochalasis can be effectively treated by surgery,and it is convenient and effective to treat dislocation of lacrimal gland and blepharochalasis through a double eyelid fold incision.This multiple surgical treatment is not only a curative operation but also a cosmetic surgery.

Cell-penetrating peptide (CPP) is a kind of small molecular peptide which can pass through a variety of cell membranes. It can carry bioactive macromolecules into cells. Due to lacking of tissue-selecting and targeting behavior, the application of CPP in the field of tumor treatment is limited. Activatable cell- penetrating peptide (ACPP) has brought the dawn to the application of CPP. This review mainly introduces the applications of ACPP in the targeting antitumor drug delivery which was designed based on the differences between tumor microenvironment and normal tissues as well as the exogenous physical stimulation.
Cell-Penetrating Peptides ; pharmacology ; Drug Delivery Systems ; Humans ; Neoplasms ; drug therapy ; Tumor Microenvironment

Objective To investigate the pharmacologic action of the Magnolia biondii Pamp volatile oil nanometer bangosome. MethodsThe pharmacologic action of the Magnolia biondii Pamp volatile oil nanometer bangosome was evaluated by observing its effect on Jimpy mice with swelling ear,capillary permeability and rats with allergic symptoms. Results The Magnolia biondii Pamp volatile oil nanometer bangosome could significantly relieve the degree of swelling in Jimpy mice extended by p-xylene,inhibit the increased capillary permeability in Jimpy mice extended by HAC,and combat the symptoms of rhinocnesmus,sneezing,nasal discharge with a better effect than the Magnolia biondii Pamp volatile oil. Conclusion The Magnolia biondii Pamp Volatile oil nanometer bangosome has a good anti-inflammation and anti-hypersensitiveness effect,which upgrades the effect of the Magnolia biondii Pamp volatile oil.

Objective To evaluate the effect for the treatment of severe asthma. Methods The data of 47 patients with severe asthma who were admitted to emergency department were retrospectively anayzed. Results Of total 47 patients ,45 were rescued, with the survival rate of 95.7%. Arterial blood gas was improved after treatment (P < 0.01). Conclusion Appropriate commencement, mode, strategy, and early weaning of mechanical ventilation, combined with the administration of bronchodilators and eorticosteroids are the important way to rescue patients with severe asthma.

Cell Line, Tumor ; Humans ; Matrix Metalloproteinase 2 ; analysis ; Matrix Metalloproteinase 9 ; analysis ; Melanoma ; enzymology ; Skin Neoplasms ; enzymology