14,and/or pH4% of the total time at 24-hour esophageal pH metry were considered to be pathologic acid reflux(GERD).All patients underwent a diagnostic test by receiving lansoprazloe 30mg or placebo twice daily for 7 days through a cross-over design.A symptom reduction of over 75% was considered positive.Results 18(17%) cases of esophagitis were found in 104 patients by endoscopy.83(80%) patients experienced a typical symptom hearburn.87(84%) patients showed pathologic acid reflux detected from pH metry and they were considered to be GERD.85(82%) of 104 patients had positive result in therapeutic test.There was a significant correlation between both the diagnosis obtained from a trial of lansoprazole and the diagnosis obtained from pH metry(P

Objective To explore the differences of H .pylori infection between residents in different humidity environment. Methods 137 persons from Sanjiang of Wenchuan and 320 persons from Chengdu were examined.Blood samples were ob-tained for serological analysis by dot immunochromatographic assay.Results The infection rate of H .pylori in Wenchuan of Sanjiang residents was 26.28%,and the Chengdu rate was 16.88% (P <0.025),with the significant difference among which,and removed the effection of nation constitution.Conclusion The differences of H .pylori infection rate between res-idents in different humidity environment were existent.High humidity environment maybe one of factors that lead to high H.pylori infection rate.

Objective To compare the serum pepsinogen (PG)in different altitude population.Methods By testing serum PG of people from different altitudes,detected the concentration of serum PG.Results The serum PGI,serum PGII and PGR were respectively 136.89±22.64 μg/L,10.05 ± 5.14 μg/L and 13.07 ± 7.41 in 1 000 m group,and they were 30.87 ± 48.66 μg/L,11.55±2.78 μg/L and 12.89±4.76 in 1 300 m group.There were significant difference in PGI and PGII(P <0.05)except PGR (P >0.05)between the two groups.The corresponding indices in 1500m group (126.64±29.84 μg/L, 12.18±5.99 μg/L and 12.2±14.84,respectively),the serum PGI was decreased obviously compared with those groups (P<0.05),the serum PGII was increased obviously compared with those groups (P < 0.05),No significant difference was found when compared with those groups (P >0.05).The serum PGI was negative correlated with higher altitudes,the ser-um PGII was positively correlated with higher altitudes.Conclusion The study found that the serum PGI content would re-duce with the increase of altitude,and on the contrary the serum PGII would rise with the increase of altitude.The main rea-son that the serum PG change may be influenced by oxygen partial pressure.

Objective To investigate serum level of thymidine kinase 1 (TK1) in middle-aged and elderly people in high humidity area,and explore the high risk age of cell abnormal proliferation and the cause.Methods Serum level of TK1 was measured with enhanced chemiluminescence in 128 cases aged 49-89 years living in the region with an average humidity of 83％.People were divided into control group (n =52,aged 49-59 years) and experimental group (n=76,aged 60 years and over).Results Serum TK1 level was lower in control group than in the experimental group [(1.3894±0.504) pmol/L vs.(1.6518±0.8685) pmol/L,t=2.159,P＜0.05].There was no significant difference in positive rate of TK1 between control and experimental groups (9.62 ％ vs.18.42％,x2 =1.894,P＞0.05).Experimental group was subgrouped into three age groups:aged 60-69 years,70 79 years and 80 89 years group.In the three age groups,serum TK1 levels were (1.7854 ±0.9736) pmol/L,(1.5345 ± 0.7039) pmol/L and (1.4420 ± 0.7354) pmol/L respectively.Compared with control group,serum TK1 level was increased in aged 60-69 years group (t=2.369,P ＜0.05),with no significant difference in serum TK1 level between control group and the other two age groups (all P＞0.05).Meanwhile,there was no significant difference in serum TK1 level among the three age groups (all P＞0.05).Conclusions Serum TK1 concentration is higher in elderly people than in middle-aged people,which shows that the abnormal cell proliferation is higher in elderly people than in middle-aged people.We should pay more attention to physical examination in the elderly people aged 60-69 years.

0.05) for penetrating blood vessel, 46% and 18%( P

Through the suvey, we find that freshmen are generally suited for college physics studies. ln this paper, high school and college students’ interest in Learnig, their initiatives, the change of their study methods and the teachers’ teaching methods are discussed.

BACKGROUND:Zinc can improve bone reaction to titanium implants through regulation of osteoblast activity under the condition of osteoporosis. OBJECTIVE:To study the zinc ions effect on improving the stability of titanium implants under the condition of osteoporosis. METHODS:After removal of the ovary, 28 female Sprague-Dawley rats presented with osteoporosis symptoms for 12 weeks and then, they were randomly divided into two groups. Two kinds of titanium implants with (experimental group) or without zinc (control group) were inserted into the bilateral femoral metaphysis longitudinal y in the ovariectomized rats, separately. Three rats were selected from each group for double fluorescence labeling examination. Mineral apposition rate was calculated at postoperatively 6 weeks. Another four rats were selected from each group for histomorphometry analysis at postoperatively 12 weeks. Biomechanical test was in the remaining rats in the two groups to calculate the maximum push-out force and maximum shear force at 12 weeks postoperatively. RESULTS AND CONCLUSION:The double fluorescence labeling examination revealed that the mineral apposition rate was improved in the experimental group as compared with the control group (P<0.05). New bone formation occurred in the two groups. However, larger new bone amount, higher bone area ratio and bone-to-implant contact were detected in the experimental group (P<0.05 or P<0.01). The maximum push-out force and maximum shear force were also superior in the experimental group to the control group (P<0.05 or P<0.01). These findings indicate that zinc ions are capable of promoting bone formation and improving implant fixation in ovariectomized rats.

Objective To analyse the clinical features of pelvic insufficiency fractures (PIF) in patients with cervical cancer after radiotherapy and explore the risk factors affecting its occurrence .Methods 55 cases of patients with cervical cancer that used radia-tion therapy as a single treatment were chosen in our hospital from February 2007 to May 2010 and the clinical features were ana-lysed;The fractures of spine ,sacrum ,sacroiliac joint ,ilium and pubis and femoral were observed and the density of fifth lumbar (L5 ) vertebrae ,left and right sacrum were detected via the CT or MRI (Hounsfield Unit)after 2-year follow-up ,the different factors on the incidence of PIF were explored .Results 12 cases (19 sites) of patients in 55 patients occured PIF after follow-up 2years ,the cumulative incidence was 21 .82% ;the distribution of PIF parts were followed :lumbar vertebrae had 5 cases ,sacral had 5 cases ,pu-bis had 4 cases ,sacroiliac joint had 2 cases ,iliac had 2 cases and femoral head had 1 case .The incidence rate of PIF that density of fifth lumbar vertebrae was ≤130 HU and >130 HU were 16 .36% and 5 .45% respectively ,the PIF′incidence rate that density of left side of sacrum was≤ -35 HU and > -35 HU were 18 .18% and 3 .64% respectively ,the incidence rate of PIF that density of right side of sacrum was ≤ -35 HU and > -35 HU were 20 .00% and 1 .82% respectively ,the density of the three parts all had statistical difference on the incidence of PIF (P<0 .05) .Univariate analysis showed that menopausal status and the average density of CT had statistical effects on PIF (P<0 .05);Multiple analysis showed that the average density of CT was the independent factors affecting PIF′incidence after radiotherapy .Conclusion The patients with cervical cancer may occur pelvic insufficiency fracture af-ter radiotherapy ,and its incidence is related to menopausal status and bone mineral density .

To prepare large naive phage antibody library, the host bacteria with high transformation efficiency is used in the Cre-LoxP recombination system. The variable regions of immunoglobulin light and heavy genes were amplified from lymphocytes collected from adult peripheral blood and newborn cord blood. The genes were spliced to form the single-chain variable fragments (scFv) by overlap PCR, cloned into pDAN5a vector and then transformed into XL2-blue MRF' with the Hte gene. Compared with XL1-blue strain, the size of the primary library was increased by 3.9 times. The primary library infected Cre recombinase-expressing bacteria, and the genes between phagemids created many new VH/VL combinations. The library was calculated to have a diversity of 1.7 x 10(11) and validated by the selection of antibodies against six different protein antigens. This library provides the basis for further selection of antibody-based drugs. It is the first time to report that XL2-blue MRF' can be used to improve the diversity of the library in the recombination system.

With the development of therapeutic antibodies over the past decade, they have become the treatment options for immunity and inflammation diseases. Major limitations of mouse antibodies as therapeutic agents - immunogenicity, lack of effectors' functions and short serum half-life -- were subsequently identified and largely overcome by the advent of humanized and fully human antibody technologies. The therapeutic antibodies for immunity and inflammatory diseases are primarily utilized in the treatment of allograft rejection, autoimmune disease, autoinflammatory syndromes, allergies and other chronic inflammation. The action mechanisms of therapeutic antibody include blocking ligands or receptors, regulating receptor activity, clearing the target cells or activating receptor. Strategies for generating the antibody drugs with high efficacy and low side effects can be realized by modulation of Fc-mediated activities and optimization of antigen-binding domains.