The abnormal changes of tumor suppressor genes such as deletion mutant,transcription inactivation or silence on human chromosome 3p (pter) are considered as a key step which is closely related to the tumorigenesis of several kinds of cancers,especially clear cell renal cell carcinoma,one kind of renal cancer.And on this basis,it has been confirmed that the deletion and downregulation of multiple 3p genes such as VHL,RASSF1A,SEMA3B,SETD2,PBRM1,NPRL2 and so on,play a vital important role in the tumorigenesis and development of clear cell renal cell carcinoma.And they provide a further way to explain the molecular mechanism of the tumorigenesis of kidney cancer.However,when the regulatory pathway and mechanism research of some genes are known to us,the other genes,especially those newly founded,which are still not clear and need to be further investigated.

OBJECTIVETo analyze rationality of internal fixation with original fixation in the second operation for femoral nonunion.

METHODSA retrospective study was conducted to analyze the clinical data of 19 femoral nonunion patients treated by internal fixation with original fixation in the second operation since January 2009 to July 2014, including 14 males and 5 females with an average age of (43.63 ± 3.95) years old ranging from 18 and 75 years old. The original fixations involved plate fixation in 11 cases and interlocking intramedullary nail in 8 cases (including 3 cases of retrograde interlocking intramedullary nails). There were 13 cases of atrophic nonunion and 6 cases of hypertrophic nonunion in pathological classification. After second operation, the fracture healing time, Harris hip score and range of motion of the knee joint was recorded and compared with preoperative.

RESULTSAfter the second operatioin, all patients were followed up for 6 to 30 months with an average of (18.67 ± 7.59) months. All patients got fracture healing, and the fracture radiological healing time was from 7 to 29 months with an average of (15.78 ± 4.97) months. Harris hip scores were improved from preoperative 62.37 ± 19.48 to postoperative 87.42 ± 8.86. The range of motion of the knee joint was improved from preoperative (52.16 ± 20.10)° to postoperative (96.32 ± 22.10)°.

CONCLUSIONIt has a better clinical healing rate for using original fixation to treat femoral nonunions, and theoretically speaking it was rationality.

Adolescent ; Adult ; Aged ; Female ; Femoral Fractures ; surgery ; Fracture Fixation, Internal ; methods ; Fracture Healing ; Fractures, Ununited ; surgery ; Humans ; Male ; Middle Aged ; Retrospective Studies

Objective To evaluate the magneto-heating effect and cytotoxicity of the carbonyl iron powder as a feasibility for arterial embolization hyperthermia. Methods Different doses of carbonyl iron powder suspensions were prepared in vitro(10 mg/ml,100%;10 mg/ml,50%;or 64 g/L phenol solutions),and heated for 20 minutes in an alternating magnetic field(49.9,79.9,and 110.2 Oe).The influences of the doses of suspensions and currency of magnetic field on the heating effect were observed.Meanwhile,mouse fibroblast L-929 cells were cultured with the suspensions for 2,4,or 7 days.The morphology and relative growth rate(RGR) of the cells were determined by microscopy and MTT assay.The cytotoxicity of the suspensions was then classified. Results The heating ability of the carbonyl iron powder increased with the suspension concentration and the strength of the magnetic field.A optimal therapeutic temperature was achieved at 110.2 Oe with 60 mg/ml carbonyl iron powder suspension.The L-929 cells showed normal morphology after been treated by the carbonyl iron powder(10 mg/ml 100% solution and 50% solution) for 2,4 and 7 days with the 0-1 degree cytotoxicity.Conclusion The carbonyl iron powder has good heating effect under the alternating magnetic field,and is compatible with the tested cells.

Objective To evaluate the imaging features of phyllodes tumor of the breast.Methods 35 patients with phyllodes tumor of the breast confirmed by surgery and histopathology were collected.All clinical and imaging findings were analyzed.Results 35 cases with 36 lesions were classified as benign (1 9 lesions),borderline (8 lesions),and malignant (9 lesions).The typical ap-pearances on mammography were ellipse (1 6/36)or lobulated (20/36)mass.Most of them were high density (30/36)and clear boundary (28/36).No evidence of calcification,spiculated margin,skin thickening,nipple retraction and enlargement of axillary lymph nodes were detected.On MRI,1 lesion presented as lobulated mass with nonuniform hypointensity on T1 WI,hyperintensity on T2 WI and DWI.The time-intensity curve was Ⅱ type on dynamic contrast enhancement MRI.Conclusion The clinical and ima-ging features of phyllodes tumor are characteristic.These features are helpful to preoperatively diagnose,the final diagnosis would confirmed by histopathology.

0.05).Conclusions There is imbalance of Th1/Th2 cells in CHB children.Th1 cytokines are positively correlated with hepatic inflammatory activity.In the course of disease,Th2 cytokines are predominant and they have a correlation to the chronic prognosis of the illness.So we can conclude the degree and prognosis of disease by observing the change of Th1/Th2 cytokines in CHB children.

OBJECTIVE To investigate the antibiotic resistance of Staphylococcus aureus isolates from the clinical samples from 2005 to 2007.METHODS The susceptibility tests and D-test were performed by the disk diffusion method and their results were analyzed by the software WHONET 5.4.RESULTS The rate of MRSA isolates accounted for 64.1%.The resistance rate to 12 kinds of the antimicrobial agents,such as penicillins,cephalosporins and carbapenems was 100%.The MRSA and MSSA were susceptible to linezolid and vancomycin.No isolates were resistant to vancomycin in our hospital;the positive rate of D-test was 78.7%.CONCLUSIONS MSSA is still sensible to most of the antimicrobial agents,but MRSA shows multidrug resistance.The D-test is of great clinical importance.

OBJECTIVETo explore treatment effect of the locking plate percutaneous external fixation to tibial fractures.

METHODSFrom July 2010 to February 2013, 8 cases with pediatric tibial fractures were treated by using unilateral locking plate percutaneous external fixation,including 6 males and 2 females with an average age of 7 years old ranging from 4 to 10. Among them, 5 cases were open fractures involving 1 case of Gustilo-Anderson type II, 3 cases of type III A, 1 case of type III B; and the other 3 cases were closed fractures involving 2 cases of AO type A3, 1 cases of type B2. The postoperative bone healing and gait impact were observed and the function was evaluated by Johner-Wruhs scores.

RESULTSAll fractures healed successfully without infection. The fracture healing time was from 3 to 6 months with an average of 3.9 months. The locking plate removal time was from 4 to 7 months with an average of 4.3 months. Among them, 7 cases were visually normal after walking with stand, 1 case of anterior tibial tendon defect affected gait. The results of Johner-Wruhs assessment were excellent in 7 cases, good in 1 case. No rub contralateral medial calf skin wounds occurenced.

CONCLUSIONThe method is simple, stable and reliable. The fixation strength is suitable for children using locking plate percutaneous external fixation. The postoperative functional recovery was excellent and the walking gait was less affected. But the point of LCP pedicle screw should be carefully selected before installation with good skin coverage.

Bone Plates ; Child ; Child, Preschool ; External Fixators ; Female ; Fracture Fixation ; methods ; Fracture Healing ; Humans ; Male ; Tibial Fractures ; physiopathology ; surgery

Adult ; Device Removal ; methods ; Ductus Arteriosus, Patent ; Heart Septal Defects, Atrial ; therapy ; Humans ; Male

Objective To investigate the action mechanism of Tianqi Yizhi Granules for Alzheimer disease. Methods Ninety SD rats were divided into sham-operation group, model group, positive medicine group, high, medium and low dosage of Tianqi Yizhi Granules groups. SD rats were injected with aggregated amyloid β1-42 protein (Aβ1-42) into their left ventricle to establish AD models. Treatment groups received gavage with Huperzine A or Tianqi Yizhi Granules. The sham-operation group and model group received gavage with the same volume of normal saline. The mitochondrial membrane potential, activity of complex Ⅰ, Ⅱ, Ⅲ and Ⅳ of mitochondrial respiratory chain, enzymatic activity of Na+-K+ATP, energy charge, and space leaning ability were detected. Results Compared with model group, Tianqi Yizhi Granules could significantly improve mitochondrial membrane potential, activity of complex Ⅰ, Ⅲ and Ⅳ of mitochondrial respiratory chain, enzymatic activity of Na+-K+ATP, space leaning ability, and energy metabolism of brain in AD rats. Conclusion Tianqi Yizhi Granules showed mitochondrial protective capacity, and could improve energy metabolism of brain in AD rats, and then improve AD rats’ space learning ability.

Sirtuin 1 (SIRT1),the Ⅲ class deacetylation enzyme,is a kind of NAD + dependent histone deacetylation enzyme.The role of SIRT1 in tumor has the duality.It can inhibit inflammation,tumor angiogenesis and interact with tumor related gene to inhibit tumor development.However,it can also regulate tumor related genes,and epithelial-mesenchymal transition,promote tumor cell proliferation and tolerance of radiotherapy and chemotherapy,and maintain the stemness of cancer stem cells to promote tumor proliferation,invasion and metastasis.