Animals ; Bone Marrow Cells ; cytology ; Cell Differentiation ; drug effects ; Combined Modality Therapy ; Drugs, Chinese Herbal ; therapeutic use ; Femur Head Necrosis ; therapy ; Humans ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; cytology ; drug effects ; Osteoblasts ; cytology ; Phytotherapy

OBJECTIVETo study the effect of Morinda officinalis (MO) extract on cytoxan (CTX) -impaired spermatogenesis of adult male SD rats.

METHODSWe randomly divided 56 adult male SD rats into seven groups of equal number: blank control, CTX model, CTX + NS, CTX + 10 g/kg MO, CTX + 20 g/kg MO, CTX + 30 g/kg MO, and CTX + 40 g/kg MO. We made the models of impaired spermatogenesis in the SD rats by intraperitoneal injection of CTX and treated the animal models by intragastric administration of MO at the concentrations of 10, 20, 30, and 40 g per kg per d, respectively. After two weeks of medication, we observed the changes in the body weight, testicular and epididymal indexes, and microstructure of the testis tissue, measured the mean seminiferous tubule diameter (MSTD) , and obtained testicular biopsy scores (TBS) in different groups, followed by comparative analyses.

RESULTSAfter treatment, the CTX + NS group showed no remarkable differences in the body weight ([234.83 ± 28.77] g) and epididymal index (2.71 ± 0.34) from those of the four CTX + MO groups, but exhibited a significantly lower testicular index ([12.15 ± 1.04] g) than those in the CTX + 20 g/kg MO ([13.71 ± 0.97] g), CTX + 30 g/kg MO, ([13.30 ± 0.29] g), and CTX + 40 g/kg MO group ([13.48 ± 0.51] g) (P < 0.05). Light microscopy revealed obvious pathological changes of the testis tissue in the CTX + NS group and significantly ameliorated structures of the seminiferous tubules in the CTX + MO 10, 20, 30, and 40 g/kg groups, with the MSTD of (204.78 ± 11.03), (216.55 ± 10.93), (218.03 ± 11.23), and (218.59 ± 14.06) μm, respectively, and the TBS of 9.03 ± 0.39, 9.69 ± 0.26, 9.83 ± 0.18, and 9.89 ± 0.11, respectively, all significantly higher than (189.74 ± 8.55) μm and 5.95 ± 1.21 in the CTX + NS group (P < 0.05). The efficacy of MO extract was increased in a concentration-dependent manner.

CONCLUSIONMorinda officinalis extract can repair cytoxan-induced damage to rat spermatogenesis, with may achieve the best effect at the concentrations of 30 and 40 g per kg per d.

Animals ; Body Weight ; drug effects ; Cyclophosphamide ; toxicity ; Epididymis ; drug effects ; Male ; Morinda ; chemistry ; Mutagens ; toxicity ; Plant Extracts ; administration & dosage ; pharmacology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Seminiferous Tubules ; drug effects ; pathology ; Spermatogenesis ; drug effects ; Testis ; drug effects ; ultrastructure

OBJECTIVE:To establish the fingerprints of 11 batches of Cardiospermum halicacabum from Guangxi,and detect its spectrum-effect relationship of antioxidant activity in vitro,and provide reference for revealing the material basis for its antioxi-dant activity. METHODS:HPLC was conducted to establish the fingerprints of 11 batches of C. halicacabum from Guangxi,the 1, 1-diphenyl-2-trinitrobenzene hydrazine(DPPH)free radical scavenging method was used to determine the antioxidant activity in vi-tro of C. halicacabum from different origins,and bivariate correlation analysis was adopted to detect the spectrum-effect relation-ship of antioxidant activity in vitro. RESULTS:The similarities of 11 batches of C. halicacabum from Guangxi were all more than 0.9,and the content change of peak 2(protocatechuic acid),3,4,12 in 16 common peaks was closely related to the DPPH free radical scavenging activity. CONCLUSIONS:The chemical composition of C. halicacabum from different origins in Guangxi is ba-sically consistent,while there are some differences in the quality of the producing area. The protocatechuic acid (peak 2) and the unknown compounds represented by peak 3,4,12 may be the pharmacodynamic material basis of its antioxidant activity.

Objective To establish a HPLC method for the simultaneous determination of campanulin, paeoniflorin and hydrox-ysafflor yellow A in Buyang Huanwu Decoction. Methods A Kromasil C18(4. 6 mm × 250 mm, 5 μm) column was adopted. The mobile phase consisited of methanol and acetonitrile(26 : 2) (A), and 0. 7 % phosphoric acid solution(B), in gradient e-lution (0～10 min, 25 % →35 % A; 10～25 min, 35 %→40 % A). The flow rate was at 1.0 mL·min-1. The column tem-perature was 35 ℃;, and the detection wavelengths were set at 260nm for campanulin, 230 nm for paeoniflorin and 403 nm for hydroxysamor yellow A. Results The linear ranges of campanuliu, paconiflorin and hydroxysafflor yellow A were 0. 0539～1. 078 μg (r=0. 999 5), O. 4160～8. 320μg (r=0. 999 8), and 0. 0418～0. 8352 μg (r=0. 999 5) respectively; the av-erage recovery was 99. 4 % (RSD=1.5 %), 100. 6 % (RSD=1.7 %), and 101.0 % (RSD=1.9 %) respectively. Conclu-sion The method is simple, feasible and reproducible, and can be used for the quality control of Buyang Huanwu Decoction.

Animals ; Arteritis ; complications ; immunology ; Coronary Artery Disease ; etiology ; pathology ; Coronary Vessels ; pathology ; Models, Animal ; Rabbits

Animals ; Biogenic Monoamines ; metabolism ; Brain ; drug effects ; metabolism ; Female ; Lipid Peroxidation ; drug effects ; Male ; Mice ; Paraquat ; toxicity

Objective To study the feasibility, superiority and experience of minimally invasive laparoscopic-assisted surgery of the thyroid using ultrosonic scalpel. Methods In a period from January, 2006-August, 2008, 56 patients of the department of general surgery, the hospital of Heilongjiang Province were diagnosed as thyroid node. The size of the nodes ranged from 5.0 cm×4.2 cm to 3.5 cm×2.0 cm in uhrosonography. An incision of about 1.5-2.0 cm was made 1.0 cm above the suprasternal notch and the operation was performed with ultrosonic scalpel assisted with laparoscope. Partial thyroideetomy of lateral thyroid was performed in 20 cases, subtotal thyroidectomy in 25 cases, subtotal thyroidectomy of unilateral thyroid and partial thyroidectomy of contralateral thyroid respectively in 1 case, partial thyroidectomy of bilateral thyroid in 10 cases. Results All 56 cases have undergone minimally invasive laparoscopic-assisted surgery successfully in 50-146 min and no complications occurred. Fifty five cases were confirmed to be benign lesion pathologically. Conclusion Laparoscopic-assisted surgery of the thyroid is an effective with a good cosmetic result.

The leading bioregional pesticide,Bacillus thuringiensis,is accepted by the public and widely used biopesticide in the world.B. thuringiensis chitinase may contribute to the biocontrol of phytopathogenic fungi and enhance insecticidal activity.It helps to take full advan- tage of Bt and upgrade the efficiency.This paper reviews the progresses of B.thuringiensis chitinase.

A combination vaccine which consists of ≥2 immunogens is intended to provide protection against two or more diseases or against multiple serotypes of a single disease. The use of combination vaccines decrease the number of vaccine injections in the regular immunization schedules. However,the development of combination vaccines is more complicated than monovalent vaccines,preservatives and adjuvants used with one component may alter the potency of other components. Physical,chemical,and biological interactions between the components of combination vaccines must be considered to avoid detrimental effects on safety or efficacy. Therefore combination vaccines present unique challenges for product evaluation. This paper presents a review of research application status,the evaluation of effectiveness and safety,as well as development prospects on combination vaccines.