Clinical studies show that the factors that impact the quality of life (QOL) in patients with lung cancer are comprehensive.Physiological factors include breathing difficulties,cancer-related fatigue.Depression,anxiety and other psychological factors also play a major role.Social and family support is also crucial to improve the QOL in patients with lung cancer.

0.05) in tail flick test.MPE% in group MK was always higher than group M and descended more slowly than group M,especially from the d4 to d8(P0.05). Conclusion Ketamine could block the development of morphine tolerance partly due to its inhibition effect on pCREB protein.

OBJECTIVE: To determinate triptolide and wilforlide A in biological samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and to verify the method. METHODS: After 0.4 mL blood, urine or 0.4 g hepatic tissues with internal standard were extracted by ethyl acetate, they were separated on a Allure PFP Propyl (100 mm x 2.1 mm, 5 µm) with a mobile phase of methanol-20 mmol/L ammonium acetate using gradient elution. For mass spectrometric detection, electrospray ionization (ESI⁺) in positive mode was elected and the data was collected using multiple-reaction monitoring (MRM). RESULTS: The linearity was good (r > 0.995 0) and the limit of detection was 2 ng/mL or 2 ng/g for triptolide and wilforlide A. The recovery was 61.08%-102.98%. The intra-day and inter-day precision was less than 12.58% for each biological sample, and the accuracy was 90.61%-105.80%. CONCLUSION This method is simple, convenient and good selective, and could be applied to analysis of triptolide and wilforlide A in different biological samples. And the method may provide technical support for forensic medicine identification, clinical diagnosis and treatment of tripterygium wilfordii Hook. f. poisoning.
Chromatography, High Pressure Liquid ; Diterpenes/urine* ; Epoxy Compounds/urine* ; Humans ; Oleanolic Acid/urine* ; Phenanthrenes/urine* ; Sensitivity and Specificity ; Tandem Mass Spectrometry

Objective To investigate the sensitivity and accuracy of monitoring oxygen saturation of arterial blood flowing through descending aorta with a probe placed in the lower segment of esophagus ( SeO2). Methods This study was approved by the ethics committee of our hospital and written consent was obtained. Forty-five ASA Ⅰ-Ⅲ patients (34 males, 11 females) aged 14-79, weighing 40-90 kg undergoing elective (36 patients) or emergency (9 patients) surgery were studied. After induction of anesthesia a probe we made (patent number ZL 200320115080.2) was placed in the lower segment of esophagus and connected to a monitor (Philips-150A) . The depth of insertion and the direction of the probe were adjusted until the same waveform as SpO2 was obtained. Once the best waveform was obtained, 10-15 min stabilization was allowed. The ventilator was then disconnected. When SeO, decreased from 100% to 90% mechanical ventilation was started again. The following parameters were recorded:(1) the onset time (the time from disconnection of ventilator to the time when SeO2 and SpO2 started to decrease), (2) the time needed for SeO2 and SpO2 to decrease from 100% to 95% (T95) and 90% (T90), and (3) the recovery time (the time needed for SeO2 and SpDO2 to return to 100% after mechanical ventilation was started again. Arterial blood samples were taken for blood gas analysis when Se02 and SpO2 were 90% , 95% and 100% . Results SeO2 , SpO, and SaO2 were 100% when the patients were being ventilated with 100% oxygen. When the ventilator was disconnected SeO, dropped much faster than SpO2. It took (286?15)s for SeO2 to drop from 100% to 95% while for SpO2 it took (398 ?28)s. The most readings of SeO2 were in the range of 2 standard deviation from SaO2. SeO2 correlated well with SaO2 and SpO2. r was 0.9754 and 0.9656 respectively(P

Antibodies, Monoclonal, Humanized ; therapeutic use ; Bevacizumab ; Electrocoagulation ; methods ; Endoscopy ; Epistaxis ; therapy ; Hemostatic Techniques ; Humans ; Male ; Middle Aged ; Telangiectasia, Hereditary Hemorrhagic ; therapy

Objective To study the relationship between urinary laminin(LN) and diabetic nephropathy.Methods Using the method for concentrating urinary laminin based on precipitation with PEG-4 000 and γ-globulin.The concentration of LN in the urine of 83 diabetics was measured by RIA.The concentration of micro-albumin and Ccr in the urine and the levels of plasma glucose and HbA1c were also measured.Results The choice of 150g/L of PEG-4 000 and 0.5 g/L of γ-globulin was the best method for concentrating urinary LN.The urinary LN in all groups was significantly higher than that in control group.Conclusion Urinary LN is an indicator in the diagnosis of the early diabetic nephropathy.

OBJECTIVE: To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for 45 poisonous alkaloids in blood. METHODS: Identification was based on the compound's retention time and two precursor-to-production transitions. The method involved a liquid-liquid extraction (LLE) followed by LC-MS/MS with multiple-reaction monitoring (MRM). When 1 mL of blood was extracted with diethyl ether at pH = 9.2 with SKF525A as the internal standard, the target compounds were analyzed with LC-MS/MS in the positive ionization mode. RESULTS: The target alkaloids had good linearity (r>0.995 1), both the intra-day precision and inter-day precision being less than 14.77%. The limits of detection ranged from 0.05 to 25 ng/mL in blood. CONCLUSION The method is selective and sensitive in detecting poisonous alkaloids with a total running time of 12 minutes; therefore it was successfully applied to some actual cases of suspected alkaloids poisoning.
Alkaloids/chemistry* ; Chromatography, Liquid/methods* ; Forensic Medicine ; Humans ; Liquid-Liquid Extraction ; Mass Spectrometry ; Sensitivity and Specificity ; Tandem Mass Spectrometry/methods*

Objective: To investigate the enhancive effect of TNF-α on transfection efficiency of adenovi-rus in mononuclear cells of mouse and the enhancive capability of protection of bone marrow by MDR1. Meth-ods: Before the mononuclear cells of mouse were infected by recombinant adenovirus encoding human MDR1 gene, they had been pretreated by TNF-α. Transfection efficiency of adenovirus was monitored by fluo-rescence microscopy, immunohistochemistry and flow cytometry (FCM). mRNA and protein levels of MDR1 in the mononuclear cells of mouse were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot before and after treatment of TNF-α. Results: After treatment of TNF-α, tansfection rates of adenovirus were obviously increased in the treated group (26.26%) compared with the untreated group (11.96%). mRNA levels and protein levels of MDR1 were obviously increased in the treated group compared with the untreated group. Conclusion: TNF-α could enhance transfection efficiency of adenovirus in mononu-clear cells of mouse and enhance capability of protection of bone marrow by MDR1.

Diagnostic Errors ; Female ; Humans ; Maxillary Sinus ; pathology ; Melanoma ; diagnosis ; Middle Aged ; Paranasal Sinus Neoplasms ; diagnosis ; Polyps ; diagnosis ; pathology