1.Effect of benzo ( a) pyrene on estrogen metabolism-related genes in endometrial cells
Wei ZHANG ; Jie YAO ; Ning GAO
Journal of Third Military Medical University 2003;0(11):-
Objective To study the molecular mechanism of benzo( a) pyrene underlying the role of estrogen metabolism-related genes in human endometrial cancer cells. Methods Effect of benzo( a) pyrene on CYP450 was studied by exposing human endometrial cancer cells ( RL95-2) to benzo( a) pyrene at various concentrations ( 0. 016,0. 8,0. 4,2. 0 and 10 ?mol/L BP) for 12 h,or to 2 ?mol/L BP for 6,12,24,36 and 48 h. RL95-2 cells were harvested and cleaved. Expressions of CYP1A1,CYP1B1 and CYP3A4 proteins in RL95-2 supernatant were detected by Western blotting. Supersome in expressed CYP450 was used as a positive control to confirm the reliability of assay methods. Results The expression of CYP1A1 protein was significantly increased in RL95-2 cells 12 h after they were exposed to different concentrations of benzo( a) pyrene or to 2 ?mol/L BP for different periods of time,and reached it peak at 48 h,indicating that expression of CYP1A1 protein depends on the used dose and time of benzo( a) pyrene. Conclusion Benzo( a) pyrene can increase the expression of CYP1A1 in human endometrial cancer cells,cause changes in estrogen metabolism,and inhibit proliferation of human endometrial cancer ells.
2.Relationship of Carotid Atherosclerosis and Coronary Artery Disease and Cerebral Infarction
jie, CHENG ; gen-fa, WANG ; wei, CHEN ; ge, YAO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(07):-
Objective To study the relationship of carotid atherosclerosis and coronary artery disease and cerebral infarction. Methods One hundred and forty patients were divided into three groups: coronary artery disease(CAD) group,cerebral infarction(CI) group and control(C) group.All the patients accepted carotid ultrasonograph. Results The incidence of multiple plaques in carotid artery was much higher in CI group than in C group and CAD group(P
3.Epidemiology study of Eales disease and its risk factors in healthy young men
Jun XIAO ; Shihui WEI ; Jie WANG ; Yao HE
Journal of Jilin University(Medicine Edition) 2006;0(01):-
0. 05), and there seemed to have some statistical correlation between the incidence of Eales disease and PPD (P
4.Application of MR Imaging Technology in Articular Cartilage of Knee Joint
Shouliang XU ; Kuilun YU ; Xinping SHI ; Jie YAO ; Wei XING
Journal of Practical Radiology 2001;0(10):-
Objective To study the MR imaging technology and 3D reconstruction of the articular cartilage of knee.MethodsConventional,2D,3D and multiparameter MR imaging scans of knee were performed in ninety patients with trauma(n=34)andosteoarthritis(n=56) of knee and 10 healthy volunteers.The articular cartilages on 3D images were reconstructed using maximum intensity projection(MIP).The mean thickness of articular cartilage was measured and analysed statistically.Results Articular cartilages of knee were clearly depicted by MR imaging technique and 3D.On 2D FSE sequence,about 50% of articular cartilage appeared as three laminar apearance.On 3D imaging,the three-dimensional morphology of articular cartilage was showed clearly.Conclusion MR imaging and 3D reconstruction of the articular cartilages of knee is helpful in early diagnosis and treatment of disordered cartilages.
5.Repair of finger pulp defect by using arterialized venous dorsal finger flap with sensory nerves
Jie ZHANG ; Changsong CHEN ; Xuhua CHEN ; Wei YU ; Binwei YAO
Chinese Journal of Orthopaedics 2012;32(4):344-347
Objective To explore a new microsurgical method for reconstruction of finger pulp defect.Methods From May 2008 to May 2009,10 male patients with finger pulp defect were treated in our hospital,aged from 18 to 38 years (average,26 years).The index finger was involved in 6 patients,the middle finger in 3 patients and the ring finger in 1 patient.All finger pulp defects were caused by machine injury.The defect sizes ranged from 1.5 cm×2.0 cm to 2.0 cm×3.0 cm.Six patients suffered from single skin defect,and 4 patinets suffered from skin defect combined with nail bed laceration and distal phalangeal fractures.All patients were performed emergency operations.The defects were reconstructed by using arterialized venous flap with microsurgical suture of the dorsal branch of the proper digital nerve.The fractures were fixed by Kirschner wires.The donor area was covered with skin grafts.Results All flaps survived completely.The fractures healed 8 to 10 weeks postoperatively.All patients were followed up for 4 to 6 months,all flaps presented satisfactory appearance,normal texture,with no pigmentation at the last followup.The static 2-point discrimination of the flaps ranged from 8 to 12mm.All injured fingers obtained good recovery of flexion and extension of the distal interphalangeal joints.The nails of the fingers with laceration of nail bed grew smoothly.The nail bed with laceration grew smoothly,and some new nails could be seen.The skin grafts applied to the donor area survived completely.Conclusion The arterialized venous flap with suture of the dorsal branch of the proper digital nerve is a good method for reconstruction of finger pulp defect,which had the following advantages:slight donor injury,low anesthesia risk,simple operative technique,and satisfactory postoperative function and appearance.
6.Research on relativity between R353Q polymorphism of the coagulation factor Ⅶ gene and cerebral infarction
Jie LIU ; Wei YAN ; Jianhua YAO ; Dongfang KAN
Clinical Medicine of China 2009;25(6):635-637
Objective To evaluate the association between R353Q polymorphism of the coagulation factor Ⅶ gene and cerebral infarction in Chinese Han people. Methods Restriction fragment length polymorphism(PCR-RFLP) methods was adopted to detect FⅦR353Q genotype and R、Q allelomorphie gone frequency of 100 cerebral infarction(CI) patients and 106 healthy people. Results There was FⅦR353Q polymorphism in the CI patients and healthy subjects. RR and RQ genotype could be found in the control group and CI group. RR and RQ genotype dis-position consistented with Hardy-weinberg equilibrium. In CI group,RR genotype was in 91 cases,RQ was in 9 cases and QQ in 0 case(the distribution frequencies were 91.00% ,9.00% and 0), and in the healthy subjects, those are 94 cases, 12 cases and 0 case(88.70% ,11.30% and 0). In the control group and CI group,R allele genotype fre-quencies were 94.33% and 95.50%, and Q were 5.67% and 4.50%. There was not significantly different in the R353Q polymorphism (χ20.3027, P=0.5822);Q allele genotype was not significantly different between the cases of control group and CI patients(χ20.2865, P=0.5925). Conclusion There are the FⅦR353Q polymorphism in Han population,however,the idea that the Q allele is a protective factor in CI is not supported.
7.Analysis of the function of the UBE gene based on bioinformatics
Yang YAO ; Jie SU ; Di WEI ; Xingmei ZHU ; Biping CHENG
Journal of Medical Postgraduates 2014;(11):1156-1159
[Abstract ] Objective Bioinformatics provides a lot of valuable information for online prediction of new genes.In this study, we predicted the biological function of ubiquitin-conjugating enzyme 2S ( UBE2S) based on bioinformatics. Methods The UBE2S gene was screened and cloned from the cDNA library of human HepG2 cells.The relationship of the structure and function of UBE2S was explored based on the full-length cDNA library.MEGA5.05, CLUSTALW2 and SWISS-MODEL were used to study the phylogeny, conservation, and 3D structure of UBE2S. Results The UBE2S gene encoded a polypeptide of 241 residues with a predicted molec-ular weight of 23 770 and an isoelectric point of 8.81.The UBE2S protein contained no transmembrane locus and the probabilities of their functions of growth factors, cation channel and structural protein were 8.904, 0.313, and 0.291.The analysis of BLASTp showed that the isolated UBE2S had a 90-97%identity with the other species. Conclusion Analysis of the structure and function of the UBE2S protein can not only provide more information about its gene family but also pave the way for further experimental studies on the molecular mechanism of the consequent hepatocellular carcinoma.
8.Study on value of IHA in diagnosis of schistosomiasis japonica
Jie ZHOU ; Wei GUAN ; Furong WEI ; Yao RUAN ; Shengming LI ; Jing XU ; Tiewu JIA ; Xiaonong ZHOU
Chinese Journal of Schistosomiasis Control 2016;28(4):375-380
Objective To evaluate the value of indirect hemagglutination test(IHA)in schistosomiasis diagnosis. Meth?ods The literature concerned schistosomiasis diagnosis with IHA in the databases of Medline,CNKI,VIP and Wanfang Data from 1982 to 2014 was collected and evaluated. Results Totally 21 articles which were satisfied with the research criteria were analyzed with the Meta?analysis method. The IHA method had high value in schistosomiasis diagnosis,the AUCSROC of IHA in laboratory evaluation was 0.990 6,while in filed evaluation was 0.832 9,and the difference between them was significant(Z=4.50,P<0.05). Conclusion The diagnosis value of IHA in field evaluation is less than that in laboratory. In the process of the elimination of schistosomiasis,developing a new and higher sensitive reagent in schistosomiasis diagnosis is needed.
9.Retrospective analysis of clinical diagnosis of 21 cases of human ehrlichiosis
Jin TIAN ; Yongwen HE ; Ping WEI ; Wei LI ; Shenghua JIE ; Jinghong YAO
Chinese Journal of Infectious Diseases 2010;28(5):278-281
Objective To investigate epidemiological and clinical features of human ehrlichiosis.Methods The epidemiological, clinical, laboratory, therapeutic and prognostic data of 21 clinically diagnosed cases of human ehrlichiosis were retrospectively analyzed. Results The epidemic regions where the ticks' activity was high located at the boundary between Hubei and Henan Provinces. All cases were farmers. The median age was 50 years ranged from 19 to 69 years. The male female ratio evident history of tick bite 1 week before the onset. The common symptoms included fever, diarrhea,cough, nausea and vomiting, abdominal pain and expectoration. The complications included hemorrhage, toxic encephalopathy, acute renal insufficiency, secondary infection and respiratory failure. The common abnormalities of routine lab data were thrombocytopenia, hypoeosinophilia,elevated lactate dehydrogenase, creatine kinase and aminotransferases, leucopenia and proteinuria.Nine cases were tested with peripheral blood smear and intracytoplasmic inclusions in neutrophils were found in one case. Seventeen cases were tested with serological assay and antibodies against Ehrlichia were positive in five cases. After doxycycline, symptomatic and supportive treatments, 14 cases were recovered and seven died. The average age of the deaths was 56 years. Conclusions Human ehrlichiosis is an acute tick-borne zoonosis and multiorgan could be involved. The older cases prone to develop complications and the prognosis is poor.
10.Differential expression of microRNA in neonatal necrotizing enterocolitis based on high-throughput sequencing
Li YANG ; Wei ZHOU ; Jie YAO ; Weiming YUAN ; Xiao RONG ; Yanbin LI ; Juan TANG
Chinese Journal of Perinatal Medicine 2017;20(1):31-37
ObjectiveTo analyze the differential expression of microRNA (miRNA) and its significance in patients with neonatal necrotizing enterocolitis (NEC).MethodsTwenty-five patients diagnosed with NEC with Bell stage≥Ⅱ, and 25 non-NEC patients as control group admitted to Guangzhou Women and Children's Medical Center between October 2014 and November 2015 were collected. White blood cells were extracted from the peripheral blood. Five samples were selected randomly each from NEC group and control group, and sequenced by second-generation Illumina high-throughput sequencing, screened for differentially expressed miRNA and analyzed for target genes prediction and biological function. The rest samples of the two groups were detected by real-time fluorescent quantitative polymerase chain reaction technology (RT-qPCR), the results were used to validate the results of high-throughput sequencing. Differentially expressed miRNA in the two groups of data was analyzed using DEGseq software.P<0.05 was considered statistically significant.P<0.01,q<0.001 and丨Log2 Ratio丨≥1 were taken as criteria for screening the differential expression. The differential expressions of miRNA in NEC group and control group were analyzed by cluster analysis using MeV4.6 software.ResultsA total of 482 miRNAs were differentially expressed in the two groups, with significant difference (P<0.05). Among them, 126 were known miRNAs with significantly differential expression in the two groups, with 58 being up-regulated and 68 being down-regulated. The results of up-regulated miRNAs (hsa-miR-223-5p,-183-3p,-222-5p) and down-regulated miRNAs (hsa-miR-23b-5p,-150-5p,-146a-3p,-1298-5p) were confirmed to be consistent with the results of sequencing. Bioinformatics analysis showed that the target genes with differential miRNA expression mainly involved Toll-like receptor signal transduction pathway, mitogen-activated protein kinase pathway, JAK-STAT and other signal transduction pathways.ConclusionsThere are significantly differential expressions of miRNAs in peripheral white blood cells of NEC neonates. These miRNs may be involved in the occurrence and development of NEC via adjusting different target genes to regulate the signal pathway.