The process of late lung development is disturbed in bronchopulmonary dysplasia (BPD).One of the keys in late lung development is secondary septation, in which secondary septa arise from primary septa, producing plenty of small alveoli, which significantly increase the surface area of gas exchange.Secondary septation,together with architectural changes to the vascular structure of the lung which minimize the distance between the blood and the inspired air, are the targets of late lung development.BPD is a disease of premature infants in which development of the alveoli is stunted caused by many factors including volutrauma,inflammation,and oxygen toxicity.Compared with early lung development, the later development of the immature lung remains unclear.This paper is to emphasize remarkable latest research of arrested late lung development and BPD.

Objective To investigate the effect of electroacupuncture plus early rehabilitation training on the function recovery after surgery for cervical spinal cord injury. Methods Sixty patients with cervical spinal cord injury undergone anterior cervical decompression and internal fixation were randomized into a treatment group and a control group, 30 cases in each group. The treatment group was intervened by electroacupuncture plus rehabilitation training, while the control group was by rehabilitation training alone. The Frankel’s grading (FG), Barthel Index (BI), and Functional Independence Measure (FIM) were adopted for evaluation before and after intervention. Results The FG was not significantly changed after intervention in both groups (P>0.05). After intervention, there was no significant difference in comparing FG between the two groups (P>0.05). The BI and FIM scores were significantly changed after intervention in both groups (P＜0.01). There were significant differences in comparing BI and FIM scores between the two groups (P＜0.05). Conclusion Electroacupuncture plus early rehabilitation training can promote the function recovery after surgery for cervical spinal cord injury, and improve the quality of life.

AIM: To discuss the complications and prevention of phacoemulification in primary angle-closure glaucoma after trabeculectomy.
METHODS:Retrospective analysis of 33 cases (37 eyes) phacoemulification in primary angle-closure glaucoma after trabeculectomy in our hospital between January 2008 and June 2012, followed up 12-33mo, intraoperative and postoperative complications were observed.
RESULTS: Compared with pre-operation, intraocular pressure hadn't increased in follow-up 6mo. Iris was injured in 5 eyes, corneal edema was in 14 eyes, anterior chamber inflammation was in 16 eyes, all symptoms were improved in 3-7d. And no case with posterior capsule rupture or vitreous loss.
CONCLUSION: Phacoemulification is an effective way for the treatment of cataract after angle-closure glaucoma trabeculectomy, careful preoperative examination, intraoperative prevention can reduce or avoid the occurrence of surgical complications.

Objective To survey the status and influence factors of radiology department nurses self-protection behavior. Methods The radiology department nurses′self-protection behavior questionnaire compiled by the researchers were used to conduct a questionnaire survey of 100 nurses in different grade hospitals. Results Scores of the item body fluid of patients with contact contaminated were wearing glovesof second-class hospital and third-class hospital were (3.695±0.73) points and (3.73±0.74) points, and there was no statistically significant different between them (P> 0.05). The other items about self-protective behavior of nurses from different levels of hospitals had some differences (t=-4.24-2.43, P<0.05). Conclusions Self-protective behavior of radiology department nurses is affected by many factors. Nursing managers should take effective measures to improve nurses′self-protective consciousness and ability.

Adult ; Case-Control Studies ; Female ; Humans ; Male ; Nervous System Diseases ; etiology ; physiopathology ; Neural Conduction ; Organophosphate Poisoning ; Pesticides ; poisoning ; Young Adult

Objective To study the effects of acute cadmium exposure on the immune function of white blood cells (WBC), plasma interleukin-2 (IL-2) and cortisol levels in rats. Methods Thirty-six male rats were randomly divided into three groups averagely. The control group (group C) and two experimental groups(groupⅠand group Ⅱ) were respectively exposed to 0, 0.5, 1.0 mg / kg body weight (BW) cadmium for 7 days by intraperitoneal injection. Blood samples of the rats were collected on the 4th and 7th day after administration of cadmium respectively and the related parameters were analyzed. Results The BW of rats in groupⅠand group Ⅱ were significantly lower than that of the group C. The WBC counts of two experimental groups were higher than that of the control group. Higher percentage of neutrophiles and lower percentage of lymphocytes were observed in rats of group Ⅰ and Ⅱ on the 7th day after cadmium exposure, while no obvious variations in monocytes(%), eosinophiles(%) and basophiles(%)were observed among the three groups. Blood T-lymphocyte(%) and IL-2 levels in rats of groupⅠandⅡdecreased on the 4th and 7th day after cadmium exposure respectively, while B-lymphocyte(%) increased on the contrary. Plasma cortisol levels in rats of groupⅠ and groupⅡ were higher than that of the group C on the 7th day after cadmium exposure. Conclusion The results showed that acute cadmium exposure could affect the WBC immune function and result in the defect of cellular immune function as well as significant change of adrenal cortex endocrine activities.

120 g?L-1. The estimated intraoperative blood loss was 1 000-1 500 ml. The patients were randomly divided into 3 groups ( n = 16 each): group Ⅰ lactated Ringer's solution (LR); group Ⅱ LR-6% HES and group Ⅲ colloid (6% HES). Blood was removed from radial artery after induction of anesthesia. The target Hct was 28% . The volume of blood removed = body weight (kg)?7.5 ? (preop Hct -target Hct) / 0.5?(preop Hct + target Hct). The removed whole blood was replaced with lactated Ringer's solution in a three to one ratio in group Ⅰ or with 6% HES in a one to one ratio in group Ⅲ. In group Ⅱ half of the removed whole blood was replaced with LR and the other half with 6% HES. The EVLW, HR, BP, Cardiac index (CI) and dp/dtmax were monitored by PiCCO and recorded before induction of anesthesia (T0), immediately after induction of anesthesia (T1), immediately after and 15 min after ANH (T2,3), immediately before and after reinfusion (T4,5) . Hct, colloid osmotic pressure and blood gases were also measured and recorded. Results The 3 groups were comparable with respect to M/F ratio, age, body weight and the volume of whole blood removed. MAP, HR, SpO2 and CVP were stable during operation in all 3 groups. Hct was significantly decreased after ANH as compared with the baseline at T0 in all 3 groups. The osmotic pressure was significantly decreased after ANH in group Ⅰ and Ⅱ and was significantly higher in group Ⅱ and Ⅲ than in group Ⅰ after ANH. CI and dp/dtmax were significantly decreased after ANH as compared to the baseline at T0 in all 3 groups. There was no significant difference in EVLW, PaO2 and [ HCO3- ] among the 3 groups. Conclusion Moderate ANH with crystalloid or colloid has little effect on EVLW and oxygenation in patients with normal cardio-pulmonary function.

Objective To investigate the action mechanism of blood-stage treatment that affects psoriasis vulgaris using observation of the Th17/Treg expression.Methods A total of 32 patients ( blood heat group,n =17 ; blood stasis group,n =15) and 15 healthy people ( control group,n =15 ) were observed.The frequencies of Th17 and Treg in peripheral blood were detected by flow cytometric analysis in patients before and after treated by heat-clearing and blood-cooling decoction,qi-enriching and blood-activating decoction.Results The ratio of Th17/Treg in peripheral blood [ blood heat group (4.21 ± 0.52 )％ ;blood stasis group( 3.32 ± 0.43 )％ ] was significantly increased in patients compared with controls [ ( 1.79 ±0.18)％ ] ( P ＜0.01 ).After herbal treatment,the ratio of Th17/Treg expression in the blood heat group [ ( 2.41 ± 0.22 ) ％ ] and in blood stasis group [ ( 2.02 ± 0.12 ) ％ ] was significantly decreased ( P ＜ 0.05 ),respectively.Conclusions Blood-stage treatment works well on Th17/Treg expression in patients with psoriasis vulgaris.

Objective To evaluate the role of the expression of protein kinase Cγ and Cα in spinal dorsal horn in the metabotropic glutamate receptor subtype 5 (mGluR5)'s participation in the development of morphine tolerance in rats. Methods Thirty-two male SD rats in which the intrathecal (IT) catheter was successfully placed were randomly divided into 4 groups (n = 8 each): control group (group C), morphine tolerance group (group M), antisense oligodeoxynucleotide (ODN) plus morphine group (group ANT) and mismatch ODN plus morphine group (group MIS). Rats in group M received 0.9% normal saline (NS) 5 μl twice a day for 8 days, and IT morphine 15 μg was injected simultaneously at 6, 7 and 8 days twice a day. Rats in group ANT and MIS received IT antisense and mismatch ODN 30 nmol (in 0.9% NS 5 μl) twice a day for 8 days respectively and IT morphine 15 μg was injected simultaneously at 6, 7 and 8 days twice a day. Rats in group C received NS instead twice a day for 8 days. Paw-withdrawl threshold (PWT) to thermal and mechanical stimulation was measured at 6, 7 and 8 days after ODN injection (T1-3). The animals were killed on 9th day (T4) and the lumbar segment of the spinal cord was removed for determination of the expression of mGluR5, PKCα and PKCγ mRNA (by RT-PCR), PKCαand PKCγ ( by Western blot). Results PWT to thermal and mechanical stimulation was significantly higher at T1.2in group M and MIS and at T1.3 in group ANT, the expression of mGluR5 mRNA, PKCα and PKCγ was significantly higher at T4 in group M and MIS, and mGluR5 mRNA expression was significantly lower at T4 in group ANT than in group C ( P ＜ 0.05). PWT to thermal and mechanical stimulation was significantly higher at T2.3, while the expression of mGluR5 mRNA, PKCα and PKCγ lower at T4 in group ANT than in group M ( P ＜ 0.05). Conclusion The expression of protein kinase C in spinal dorsal horn plays an important role in the mGluR5's participation in the development of morphine tolerance in rats.

Objective To explore the application of bumetanide to inhibition of tumor cell proliferation.Methods In different cell lines, the expression of natrium,kalium, chloride cotransporter 1 ( NKCC1) was detected by Western blotting while the proliferation of different tumor cells was examined by CCK-8 kit.Results The target protein NKCC1 expression in lung cancer cell line ( A549 ) and colorectal cancer cell line ( HCT116 ) was significantly higher than that in chronic myelogenous leukemia cell line (K562), esophageal cancer cell line (Eca109), cervical carcinoma cell line (HeLa), T lymphocytic leukemia cell line (Jurkat) and breast cancer cell line (MCF7).IC50 Values of bumetanide were significantly lower in A549 and HCT116 than in K562, Eca109,HeLa,Jurkat and MCF7.Furthermore, the inhibiory rate and the target protein expression level were positively correlated.Conclusion Bumetanide can inhibit tumor cell proliferation and NKCC1 can serve as a potential target of anticancer drugs.