Abstract?AlM:To study the thickness changes of retinal nerve fiber layer ( RNFL) in Parkinson's disease ( PD) .?METHODS:Fifteen eyes of 15 PD patients in early stage and 18 eyes of 18 controls were chosen to take RNFL examination by optical coherence tomography ( OCT ) . Circular scans were taken around the optic nerve head (diameter = 3. 46mm) to record the features of RNFL. Scanning areas included eight quadrants of the temporal, superior, nasal, inferior, inferior-temporal, superior-temporal, inferior-nasal and superior-nasal quadrant. RNFL thickness was comparatively analyzed in eight quadrants and the average level in two groups.?RESULTS: RNFL thickness in two groups in temporal, superior, nasal , inferior, temporal- inferior, temporal-superior, nasal-inferior, nasal-superior were: ( control/PD) 83. 2 ± 17. 5μm/68. 7 ± 13. 5μm, 132. 7 ± 17. 4μm/128. 1 ± 25. 3μm, 83. 7 ± 22. 3μm/76. 5 ± 17. 8μm, 141. 5 ± 15. 3μm/128. 6± 13. 2μm, 117. 9 ± 24. 5μm/103. 3 ± 14. 1μm, 120. 8 ± 21. 2μm /102. 6 ± 23. 7μm, 110. 2 ± 27. 7μm/96. 6 ± 15. 0μm, 109. 6 ± 20. 6μm/101. 2 ± 20. 9μm. The average RNFL thickness in controls was 109. 9 ± 8. 5μm while it showed 102. 3 ± 11. 9μm in PD group. RNFL thickness in inferior, temporal, inferior-temporal, superior-temporal and the average RNFL thickness were statistically different in two groups ( t= 2. 595, 2. 700, 2. 153, 2. 330, 2. 131;P= 0. 014, 0. 011,0. 040,0. 026,0. 041).?CONCLUSlON: RNFL thickness in inferior, temporal, inferior temporal, superior temporal and the average RNFL thickness are significantly thinner in mild PD than those in the controls.

OBJECTIVE:To develop a HPLC method for determination of the content of baicalin in Kugan gran?ules.METHODS:ODS-C 18 column was used and methanol-water-acetic acid(45∶55∶1)was used as mobile phase and UV detection wavelength was274nm.RESULTS:There was a good linear relationship between the concentration of Baicalin and absorption area value in range of0.25?g～2.5?g r=0.9992.The average recovery was98.4%with RSD=1.09%(n=5). CONCLUSION:The method was highly repeatable and accurate.

In order to study the enhancement of immune functions and autologous tumor-killing (ATX) activity by kappa-selenocarrageenan (KSC) in mice bearing sarcoma 180, the effects of KSC and/or Cyclophosphamide (Cy) on natural killer(NK) activity, lymphokine activated killer(LAK) activity, the production of interleukin-2 (IL-2), ATK activity and the growth of sarcoma 180 (S180) were observed. The results showed that KSC promoted NK activity, LAK activity and ATK activity in vivo, increased IL-2 production at 40mg/kg ?d x 9d. It also enhanced the antitumor action of Cy (20mg/kg?d x 9d) and offsetted the inhibition of Cy on immunocompetent cells. The ATK activity in splenocytes of SI80-bearing mice could be induced and augmented by recombinant interleukin-2 (rIL-2) in vitro. In conclution, KSC had a up-regulating effect on immune functions and ATK activity in tumor-bearing mice, therefore, can be used as a biological response modifier (BRM) in cancer biotherapy.

BACKGROUND:Recent development of bioengineering technology and tissue-engineered nerve brings a new hope for the treatment of peripheral nerve injuries, which has gradual y become a research spot. OBJECTIVE:To review the new progress in the repair of peripheral nerve injuries using seed cells, biomaterials and tissue-engineered nerve construction technology. METHODS:PubMed and CNKI were searched by the first authors for articles concerning nerve tissue engineering and repair of peripheral nerve injuries published prior to July 2013. The keywords were“tissue engineering, peripheral nerves, nerve injuries, stem cells, Schwann cells, scaffold, growth factor”in English and Chinese, respectively. The articles published recently or in the authorized journals were preferred in the same field. Final y, 63 articles were included in result analysis. RESULTS AND CONCLUSION:Up to now, there is a great advance in the tissue engineering technology for the repair of peripheral nerve injuries. However, most studies are stil in experimental step. For the clinical application of nerve tissue engineering, some problems to be solved include:(1) source and ethics of seed cells;(2) immunological rejection fol owing cellproliferation and transplantation;(3) stability and oncogenicity of transplanted cells;(4) degradation rate, optimal porosity, tube thickness and shape;(5) repair timing for in vitro tissue-engineered nerve construction;(6) local release and regulation of various neurobiological factors. With the development of science, many patients with nerve injuries can profit from the solve of these problems.

Objective To investigate the changes of CD11b on neutrophils during myocardial ischemia-reperfusion injury. Methods 12 patients undergoing coronary bypass grafts under CPB were studied. Blood samples were obtained from the coronary (coronary sinus) and systemic circulation (central venous and CPB machine), respectively. Samples obtained from coronary and systemic circulation were taken before the beginning of CPB, during CPB and after cardiac reperfusion. Neutrophil CD11b expression and serum cTnI concentration were measured. Results (1)Expression of CD11b increased gradually in systemic circulation during CPB and after reperfusion (P

Objective To explore the characters of cognitive dysfunction for first-ever lacunar infarction (LI). Methods 136 first-ever diagnosed LI patients were evaluated with Alzheimer's disease assessment scale (ADAS) 72 h, 2 weeks, 1 month, 3 months and 6 months after attack. The scores were compared with the baseline and those of 76 normal control. Results Memory: during the first 72 h, significant difference was shown between the every location of LI (including the basal ganglia, corona radiate,frontal lobe, temporal lobe,parietal lobe, periventricular location, centrum semiovale, thalamus) and normal control (P<0.05); but between the certain location of LI and that of normal control 2 weeks, 1, 3 and 6 months after attack (P<0.05). Language: there was significant difference between every location of LI and normal control 72 h, 2 weeks and 1 month after attack (P<0.05), but between the certain location of LI and that of normal control 3 and 6 months after attack (P<0.05). Practice: there was significant difference through every visit time point of all the LI patients compared with normal control (P<0.05). Attention: Significant difference (P<0.05) was found between every location LI patients and normal control 72 h after attack, but between certain location LI patients and normal control 2 weeks, 1, 3 and 6 months after attack (P<0.05). Orientation:LI was insufficient to cause to decline in orientation. Conclusion There are mild to severe cognitive dysfunctions in the first-ever diagnosed LI patients, which need pay attention to and intervene in time.

Objective To investigate clinical biologic characteristics and factors that impact prognosis of patients with follicular thyroid carcinoma(FTC).Method Clinical data of 105 FTC patients treated surgically in the Department of Head& Neck,Tianjin Cancer Hospital from 1970 to 1990 was analyzed retrospectively.Results The overall 5-year,10-year and 15-year survival rates of these patients were 85.3％,76.7％ and 72.9％ respectively.The overall 15-year survival rates of patients ≥45 years and those ＜ 45 years were 45.9％,89.8％ respectively.The overall 15-year survival rates of patients with unilateral and bilateral carcinoma was 50.0％,76.2％ respectively.The overall 15-year survival rates of patients with neck lymph node metastasis and without were 54.2％,79.2％ respectively.The overall 15-year survival rates of patients at stage Ⅰ,stage Ⅱ,stageⅢ and stageⅣ were 89.3％,70.0％,45.5％ and 35.3％respectively.During the follow-up period,11 patients were diagnosed with distant metastasis from 1 year to 33 years after surgical treatment and 9 died of cancer within 5 years after diagnosis.Seventeen patients had local recurrence from 3 months to 34 years after surgery and 10 of these patients died of local recurrence.Conclusions The factors influencing prognosis of patients with FTC were age,clinical stage,bilateral carcinoma and neck lymph node metastasis,therefore early treatment and close following-up are essential to improve the prognosis of patients with FTC.

Aged, 80 and over ; Chronic Disease ; Female ; Humans ; Mucor ; pathogenicity ; Mucormycosis ; microbiology ; pathology ; Orbit ; pathology ; Sinusitis ; microbiology ; pathology

OBJECTIVE To investigate the antibiotic resistance of Staphylococcus aureus isolates from the clinical samples from 2005 to 2007.METHODS The susceptibility tests and D-test were performed by the disk diffusion method and their results were analyzed by the software WHONET 5.4.RESULTS The rate of MRSA isolates accounted for 64.1%.The resistance rate to 12 kinds of the antimicrobial agents,such as penicillins,cephalosporins and carbapenems was 100%.The MRSA and MSSA were susceptible to linezolid and vancomycin.No isolates were resistant to vancomycin in our hospital;the positive rate of D-test was 78.7%.CONCLUSIONS MSSA is still sensible to most of the antimicrobial agents,but MRSA shows multidrug resistance.The D-test is of great clinical importance.

OBJECTIVEThis study measures the glutaredoxin (Grx) gene and protein expression in umbilical vein endothelial cells upon exposure to Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS). The involvement of the Akt-signaling pathway is also determined.

METHODSEA-hy926 cells were pretreated with 1,000 ng · mL⁻¹ P. gingivalis LPS for 4, 12, 18, and 24 h, and then real-time reverse transcription polymerase chain reaction was employed to detect Grx1 expression. The effect of Grx on Akt activity was investigated using Western blot for the control, LPS (1,000 ng · mL⁻¹ LPS), and carmus- tine (BCNU) groups (1,000 ng · mL⁻¹ LPS, and the EA-hy926 cells were pretreated with 25 μmol · ml⁻¹ BCNU for 30 min).

RESULTSGene expression of Grx1 significantly increased in LPS group compared with that in the control group. The Grx1 expression reached the peak level in 12 h, and the variation between the expression in 4 and 12 h was significant (P < 0.05). After 12 h, the protein levels of Grx and phosphorylated-Akt (p-Akt) significantly increased in the LPS group (P < 0.05), whereas the BCNU group showed a considerable decrease in both Grx and p-Akt expression levels (P < 0.05). Moreover, a slight difference was observed in the total Akt protein levels in the three groups (P > 0.05).

CONCLUSIONGrx expression increased upon exposure of EA-hy926 cells to the LPS. Akt activity could be inhibited by BCNU (a Grx inhibitor), which indicated that Akt might act as a downstream regulator of Grx.

Endothelial Cells ; Glutaredoxins ; genetics ; Humans ; Lipopolysaccharides ; pharmacology ; Oxidative Stress ; drug effects ; Phosphorylation ; Porphyromonas gingivalis ; pathogenicity ; Proto-Oncogene Proteins c-akt ; drug effects ; Signal Transduction ; drug effects ; Umbilical Veins