2.Expressions of chemokine CXCL12 and its receptor CXCR4 in human sperm.
Yan WANG ; Wei SHA ; Wen-hui ZHOU
National Journal of Andrology 2015;21(3):225-228
OBJECTIVETo explore the expression patterns of the chemokine CXCL12 and its receptor CXCR4 in human sperm.
METHODSWe collected semen samples from 10 fertile men, performed density gradient centrifugation, and then determined the expressions of both CXCL12 and CXCR4 in the sperm by RT-PCR and immunofluorescence staining.
RESULTSRT-PCR revealed the mRNA expressions of CXCL12 (0.641 +/- 0.180) and CXCR4 (0.464 +/- 0.100) in the sperm. However, only CXCR4 rather than CXCL12 was expressed at the protein level, and the positive staining for CXCR4 was observed mainly in the posterior part of the acrosome.
CONCLUSIONCXCL12 and CXCR4 are involved as important molecules in regulating the function of human sperm.
Acrosome ; metabolism ; Centrifugation, Density Gradient ; Chemokine CXCL12 ; metabolism ; Humans ; Male ; Receptors, CXCR4 ; metabolism ; Signal Transduction ; Spermatozoa ; metabolism
3.Eosinophils and related diseases.
Yu-wei DI ; Yan-hui LIU ; Heng-guo ZHUANG
Chinese Journal of Pathology 2009;38(7):499-502
Antineoplastic Agents
;
therapeutic use
;
Autoimmune Diseases
;
pathology
;
Benzamides
;
Cell Differentiation
;
Cell Movement
;
Cell Survival
;
Eosinophilia
;
chemically induced
;
pathology
;
Eosinophils
;
cytology
;
physiology
;
Helminthiasis
;
pathology
;
Humans
;
Hypereosinophilic Syndrome
;
drug therapy
;
pathology
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Hypersensitivity
;
pathology
;
Imatinib Mesylate
;
Piperazines
;
therapeutic use
;
Pyrimidines
;
therapeutic use
4.Selection points by up-down cross method for ankle sprain.
Cheng LI ; Wei-Hui LU ; Jing-Yan XU
Chinese Acupuncture & Moxibustion 2011;31(10):918-918
Acupuncture Points
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Acupuncture Therapy
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Adolescent
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Adult
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Ankle Injuries
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therapy
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Female
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Humans
;
Male
;
Sprains and Strains
;
therapy
;
Treatment Outcome
;
Young Adult
5.Efficacy of Capparis Spinosa extracts in the treatment of scleroderma:an experimental study in animal models
Xinling MI ; Fan LI ; Yan HUI ; Wei HUA ; Kejian YANG
Chinese Journal of Dermatology 2008;41(7):469-472
Objective To observe the effect of ethanol extract and ethyl acetate extract of Capparis Spinosa on the thickness of dermis,synthesis of collagen type Ⅰ,type Ⅲ,and expression of transforming growth factor-β1 in mouse models of scleroderma.Methods Mouse models of scleroderma were established through local injection of bleomycin on the back once a day for 4 weeks.After confirmation of model establishment,72 mouse models were equally and randomly divided into three groups.Two groups received topical treatment with ethanol extract of Capparis Spinosa and ethyl acetate extract of Capparis Spinosa,respectively,no treatment was given to the rest of the control group.After 2-,4-,6-week treatment,8 mice were sacrificed and tissue samples were obtained from the back,and subiected to the measurement of dermal thickness by HE staining,as well as to the analysis of expression of collagen type Ⅰ,collagen type Ⅲ and transforming growth factor-β1 by immunohistochemical staining.Results On week 2,4,6,the thickness of dermis was 23.22,24.94,19.97 μm respectively in mice treated with ethanol extract of Capparis Spinosa,27.66.26.15,22.13 μm respectively in those treated with ethyl acetate extract of Capparis Spinosa.Compared with the mouse models without treatment,the thickness of dermis significantly decreased(F=12.99,P<0.01),the expression of collagen type Ⅰ(F=7.47,P<0.01)and transforming growth factor-β1(F=11.76,P<0.01)were also inhibited in those receiving treatment.However,the expression of collagen type Ⅲ was not affected obviously by the treatment.Conclusion The ethanol extract and ethyl acetate extract of Capparis Spinosa have the effect against skin fibrosis.
6.Effect of CYP3A and P-glycoprotein on the absorption of buagafuran in rat intestinal lumen.
Li SHENG ; Wei TAN ; Jinping HU ; Hui CHEN ; Yan LI
Acta Pharmaceutica Sinica 2010;45(1):43-8
The rat single-pass intestinal perfusion model was applied to study the effect of CYP3A and P-glycoprotein on the absorption of buagafuran in lumen of rats. Buagafuran concentrations in intestinal perfusate and blood in vena mesenterica collected at different time points after perfusion were determined by GC-MS. Permeability coefficient of buagafuran was calculated by the equation [P(lumen) = -(Q/2pirl)Ln(C(out)/C(in)) and P(blood) = (deltaM(B)/deltat)/(2pirl
7.Investigation on the short-term clinical application of two types of glass fiber posts.
Hui SONG ; Jingwen WANG ; Xuliang DENG ; Yan WEI
West China Journal of Stomatology 2014;32(4):390-393
OBJECTIVEThe clinical results of restoring defective teeth with two types of glass fiber prothetic systems is investigated to acquire clinical experience for further application of glass fiber posts with independent intellectual property rights.
METHODSA total of 120 out-patients with restored defective teeth were selected from the Department of Stomatology, Beijing Xuanwu Hospital of Traditional Chinese Medicine and Peking University School and Hospital of Stomatology and randomly divided into two groups. OUYA FIBER posts and Tenax Fiber White posts were used to restore defective teeth in the experimental group and the control group, respectively. Clinical evaluation was conducted one week and three months post-restoration.
RESULTSBoth clinical satisfactory rates of OUYA FIBER posts and Tenax Fiber White posts were higher than 98% one week post-restoration and higher than 96% three months post-restoration. No significant differences were observed between the clinical results of restoring defective teeth with the two types of fiber posts. Throughout the healing process, no root canal fracture or marginal staining were observed.
CONCLUSIONOUYA FIBER post and Tenax Fiber White post showed similar clinical outcomes during the period of observation in this study. However, the long-term effects should be observed in future studies.
Dental Stress Analysis ; Glass ; Humans ; Post and Core Technique ; Resin Cements ; Tooth Fractures
9.The experimental research anti-tumor effect of modified decoction for driving out blood stasis in the blood mannsion
Yan LI ; Zhonglong MA ; Wei TIAN ; Hui LIU
International Journal of Traditional Chinese Medicine 2011;33(5):405-407
Objective Modified Decoction for Driving Out Blood Stasis in the Blood Mannsion on ascites tumor model of transplanted H22 anti-tumor effect and the impact of VEGF. Methods Adult male mice 100, inoculated with H22 hepatoma cells, the establishment of ascites H22 transplanted tumor model, then divided into 10 groups were given saline, capecitabine, flavored Modified Decoction for Driving Out Blood Stasis in the Blood Mannsion (high, medium and low dose) was administered orally for 10 days, 11 days of treatment, observation of suppression tumor rate, the rate of change in life extension; detected by immunohistochemistry the expression of VEGF in tumors, SPSS13.0 software for statistical analysis. Results Capecitabine, flavored Xuefuzhuyutang (high, medium and low dose) inhibited tumor growth rates were 60%, 49%, 41%, 35%, life extension rate of the three groups were 1.68% 157.98%, 70.58%, 49.57% higher. Conclusion Modified Decoction for Driving Out Blood Stasis in the Blood Mannsion can inhibit tumor cell proliferation in tumor-bearing mice, significantly prolonged the survival time of mice, reduce the tumor tissue and tumor tissue expression of VEGF.
10.Advances in antiviral therapy targeting to hepatitis C virus NS3/4A protease
Yan GUO ; Changye HUI ; Wei XIONG ; Wen ZHANG
Chinese Journal of Clinical Infectious Diseases 2014;7(1):89-93
Hepatitis C virus (HCV) genome is of high variation,which results in persistent infection of HCV and increases the incidence of liver cirrhosis and hepatocellular carcinoma.Following the successful paradigm established for HIV protease inhibitors,HCV NS3-4A serine protease has been selected as the main target for the development of small molecule antiviral agents.In this article,we review recent progress in the discovery and development of HCV NS3-4A protease inhibitors,and discuss their antiviral activities,pharmacokinetic properties,side effects and resistance profiles.