Objective: To study the periapical microleakage of the root canals filled with four kinds of sealers using two obturation techniques. Methods: Sixty-four extracted first maxillary premolars were randomly divided into four groups. All the root canals were prepared with Protaper rotary instruments and filled with two obturation techniques: warm vertical condensation and cold lateral condensation. Four kinds of sealers included: Cortisomol, nano-zinc oxide, Apexit and calcium hydroxide which were indivi-dually used for four groups. After the teeth were dyed for 7days with Indian ink, dyeing lines were measured with digital camera under steromicroscope to reflect the microleakage. Results: In cold lateral condensation groups, dyeing lines of calcium hydroxide group had an average length of (1.75?0.22) mm, which was significantly longer than that of Cortisomol group (1.40?0.15 mm) and nano-zinc oxide group [(1.56?0.25) mm, P0.05], which were significantly shorter than that of cold lateral condensation groups (P

Aged ; Atropine ; Electrocardiography ; Female ; Heart Rate ; drug effects ; Humans ; Male ; Muscarinic Antagonists ; pharmacology ; therapeutic use ; Preoperative Care ; methods ; Quinuclidines ; pharmacology ; therapeutic use

Autonomic Nervous System Diseases ; metabolism ; pathology ; surgery ; Colon ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Enteric Nervous System ; abnormalities ; pathology ; Hirschsprung Disease ; pathology ; Humans ; Infant, Newborn ; Intestinal Diseases ; metabolism ; pathology ; surgery ; Male ; Phosphopyruvate Hydratase ; metabolism ; S100 Proteins ; metabolism

Amiodarone ; adverse effects ; Female ; Humans ; Middle Aged ; Muscular Diseases ; chemically induced

Actinomyces ; isolation & purification ; Actinomycosis ; microbiology ; Apicoectomy ; methods ; Candida albicans ; isolation & purification ; Candidiasis ; microbiology ; Enterococcus faecalis ; isolation & purification ; Foreign Bodies ; complications ; Gram-Positive Bacterial Infections ; microbiology ; Humans ; Microsurgery ; methods ; Periapical Periodontitis ; etiology ; microbiology ; surgery ; therapy ; Radicular Cyst ; complications ; Root Canal Filling Materials ; therapeutic use ; Root Canal Therapy ; methods

Adult ; Arthroplasty, Replacement, Hip ; Bone Cysts, Aneurysmal ; pathology ; Bone Neoplasms ; metabolism ; pathology ; surgery ; Chondroblastoma ; pathology ; Chondrosarcoma ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Femur Head ; metabolism ; pathology ; surgery ; Follow-Up Studies ; Humans ; Osteosarcoma ; pathology ; S100 Proteins ; metabolism

ATP-Binding Cassette Transporters ; immunology ; metabolism ; Animals ; Herpes Simplex ; immunology ; virology ; Herpesvirus 1, Human ; immunology ; metabolism ; Humans ; Immediate-Early Proteins ; immunology ; metabolism ; Viral Proteins ; immunology ; metabolism

Objective To compare enhancement features of hepatic tumors on SonoVue-enhanced real-time gray-scale ultrasonography and contrast-enhanced helical CT.Methods SonoVue-enhanced real-time gray-scale ultrasonography and contrast-enhanced helical CT were performed on 49 patients with 54 focal liver lesions,including 29 primary liver carcinomas,4 metastatic liver tumors,11 hepatic carvernous hemangiomas,7 focal nodular hyperplasias,2 focal fatty sparing,1 cirrhotic.Results Contrast-enhanced ultrasonography and helical CT showed consistent results in demonstrating hemodynamics of hepatic tumors.In the differentiating of malignant tumors and benign tumors,the sensitivity,specificity and accuracy of contrast-enhanced ultrasonography were(90.9)%(30/33),(90.5)%(19/21) and(90.7)%((49/54)),respectively.Those of contrast-enhanced CT were(90.9)%(30/33),(95.2)%(20/21) and(92.6)%((50/54)),respectively.Conclusions Both of the two imaging methods are valuable for differential diagnosis of liver tumors.

Objective To observe the changes of platelet activation markers in newborn infants with hyperbilirubinemia,and its relationship with myocardial enzymes and clinical significance.Methods Thirty neonates with hyperbilirubinemia were selected as observation group,and 15 health newborns were served as normal control group.In the morning,1 mL fasting blood in the femoral vein was collected from the patients in both groups,and 20 g/L EDTA-Na2 0.1 mL anticoagulant was added with a gentle shake.CD41-FITC 10 ?L was injected into two test tubes,with IgG1-PE and CD62-PE reagent 10 ?L added,diluted liquid 200 ?L PBS,and with 5 ?L whole blood,under dark room temperature for 15 minutes.Negative control tubes(CD41-FITC plus IgG1-PE) were applied to adjust voltage,the flow cytometry was used to determine CD62-PE.Myocardial enzymes and liver function were measured by automatic biochemistry analyzer.Results The levels of platelet activation markers in observation group were significantly higher than that in the normal control group(P

OBJECTIVE:To establish a method for simultaneous determination of valsartan and hydrochlorothiazide in Valsar-tan hydrochlorothiazide tablets. METHODS:UPLC was adopted. The determination was performed on Phenomenex C18 column with mobile phase consisted of [0.1% phosphoric acid solution-acetonitrile(95 : 5,V/V)]-[0.1% phosphoric acid solution-acetonitrile (5 : 95,V/V)](gradient elution)at the flow rate of 0.25 mL/min. The detection wavelength was set at 272 nm,and the column tem-perature was 35 ℃. The sample size was 1.5 μL. RESULTS:The linear range were 8.1-324.2 μg/mL for valsartan(r=0.9999)and 1.2-50.1 μg/mL for hydrochlorothiazide(r=0.9999). The limits of quantitation were 0.24,0.04 ng,and the limits of detection were 0.06,0.01 ng. RSDs of precision,stability and reproducibility tests were less than 2.0%;recoveries were 97.69%-100.35%for valsartan(RSD=1.03%,n=9)and 98.27%-100.60% for hydrochlorothiazide(RSD=0.83%,n=9). CONCLUSIONS:The method is simple,rapid and accurate,and can be used for the simultaneous determination of valsartan and hydrochlorothiazide in Valsartan hydrochlorothiazide tablets.