In this article we collected 2 embryos and 69 fetuses between 7 and 30 weeks of gestational age and 3 neonates to study the development of the human stomach by histological, histochemical and immunogold-siver methods. In 7-week embryo, the superficial layer of gastric mucosa was stratified columnar epithelium, containing a large amount of glycogen. In 9-week fetus, simple columnar epithelium, gastric pits and glandular buds were observed. At this stage a few parietal cells could be identified at the bottom of the glands. The pyloric glands contained parietal cells as fundic glands. At 13-14 week the muscularis mucosa appeared and the wall of stomach formed definitively as the adult. A few argyrophil cells in antrum and fundus were found at 12-week fetus. They scattered in the surface epithelium and concentrated in the lower portion of the glands. The argyrophil cells were round, pyramidal or spindle in shape. More argyrophil ceils were found in the antrum from 14-week on. At 18-week, the argyrophil cells were most numerous. Some cells possessed processes extending to the basement membrane or parietal cells. Between 15-30 weeks various shaped EC cells in fundus were found, with some open-type endocrine cells. G cells in antrum were mostly rounded and often in groups at 13,16 and 21 week. Developing G cells were observed under EM.

AIM: To construct the eukaryotic expression vector CD80-IgG by fusing the cDNA encoding extracellular portion of murine CD80 to the 5'-terminus of cDNA encoding Fc fragment of murine immunoglobulin G1 and to express the fusion protein in Chinese hamster ovary (CHO) cells. METHODS: The two cDNAs was amplified by PCR respectively from plasmid pcDNA/B7 containing the full-length cDNA of murine CD80 from murine spleen cells, and cloned to the eukaryotic expression vector pcDNA3.0 by directional cloning. The resultant recombinant plasmid pcDNA/CD80-IgG was transfected into CHO cells with liposome transfection reagent. The stably expressing cells were obtained by G418 screening. Western blot, Dot ELISA, and flow cytometry were used to detect the expression of the fusion protein and its immunological activity. RESULTS: DNA sequencing verified the correction of the construction of recombinant plasmid pcDNA/CD80-IgG. The expressed fusion protein was detected in the supernatant of transfected CHO cells and the molecular weight of the protein was similar to what we expected. Its immunological activity was also established. CONCLUSION: The recombinant plasmid pcDNA/CD80-IgG was successfully constructed and it expressed the fusion protein CD80-IgG.

ObjectiveTo evaluate the therapeutic efficiency and outcomes of vertical condensation of warm gutta-percha obturation using the four-handed technique.MethodsA split-tooth model constructed with lateral grooves and depressions was used to compare vertical condensation of warm gutta-percha obturation with and without the four-handed technique.Respectively 10 times of obturation were done in the four-handed group and the independent operation group.The operation time and defect replication quality were recorded.Evaluation of defect replication quality was on an ordinal scale 0 to 2 grade based on how much each defect was replicated.The results were statistically analyzed.ResultsThere were signiticant differences in treatment time between the four-handed group and theindependent operation group,as well as in treatment outcomes between two groups.ConclusionsUse of the four-handed technique can improve therapeutic efficiency and outcomes of vertical condensation of warm gutta-percha obturation.

Epigenetic abnormalities not only associated with carcinogenesis, they may also influence the curative effect and prognosis of anticancer drugs through modifying pharmacokinetic genes related to drug absorption, distribution, metabolism, excretion and pharmacodynamic genes related to signaling pathways and targets. That drugs through regulating epigenetic factors pocessing anti-cancer effect is becoming a research hot spot. We summarized and analyzed the realtionship of DNA methylation, miRNA, and histone modification with antitumor drug effect, aiming at promoting rational use of antitumor drugs and providing new ideas on developing epi-drugs.

This paper discusses the meaning of the tutorial system on clinical practice;the qualifications,tasks and a responsibility of the tutors,expounds the role of tutors,and discusses the main problems of tutorial system of the undergraduate.

Objective To investigate the effect of tacrolimus on the expression of nuclear factor-κB (NF-κB) in HaCaT cells stimulated by tumor necrosis factor-α(TNF-α),and on the expression of glucocorticoid receptor (GR)α and β in untreated HaCaT cells in vitro.Methods Cultured Ha CaT cells were treated with TNF-α(10μg/L) only,combination of TNF-α(10μg/L) and various concentrations (10-8mol/L, 10-7mol/L,10-6moL/L) of tacrolimus or tacrolimus of different concentrations only.After additional 12-,24-, 36- or 48-hour cnlture, Westem blot and immunofluorescenee-confocal laser scanning microscopy were used to detect the expressions of NF-κB,GRα and GRβ in HaCaT cells.Those untreated HaCaT cells served as the control.Results The relative protein expression level of NF-κB was increased in HaCaT cells after treatment with TNF-α for 24 and 48 hours zompared with untreated ceils (0.73±0.0316 and 0.8925±0.0171 vs 0.4988±0.03506,both P<0.05);however,the increase in NF-κB expression was inhibited by the combination treatment with tacrolimus,and the relative expression level of NF-κB protein was 0.6825±0.0263.0.6200±0.0163 and 0.5575±0.0299 in HaCaT cells treated with TNF-α plus tacrolimus of 10-8mol/L 10-7mol/L and 10-6mol/L,respectively;the difference was significant etween TNF-α-treated cells and those dealt with the combination of NF-α and tacrolimus of 10-7 or 10-6 mol/L (both P<0.05).No significant difference was observed in the expression of NF-κB by HaCaT cells between different time oints treated with tacrolimus of 10-8,10-7 or 10-6 mol/L.Also,there was no zignificant difference in the expression of GRα or GRβ between untreated HaCaT cells and those treated with tacrolimus of 10-8, 10-7 or 10-6 mol/L at any time point.Conclusions Tacrolimus ould inhibit the expression of NF-κB by TNF-α-stimulated HaCaT cells,but does not affect the expression of GRα or GRβ,in untreated HaCaT cells.

Objective To evaluate the changes of β cell function in critically ill surgical patients and its re- lationship with prognosis.Methods 32 patients>16 years age with no history of diabetes who were admitted to sur- gical intensive care unit(SICU)were divided into two groups according to APACHE Ⅱ score.Blood sample Was taken on preoperative and postoprative 1st day for measures of fasting insulin(FINS)and fasting C peptide(FCP). The HOMA-β Was calculated.Results The level of FINS,FCP and HOMA-β were significantly decreased on first day after operation than preoperative in critical group.Compared with control group,The level of FINS.FCP and HO- MA-βwere significantly reduced on first day after operation-which had negative correlation with APACHE Ⅱ score, APACHE Ⅲ score and in charge days of ICU.Conclusion There is β cell dysfunction in critically ill Surgical pa- tients.β cell function in surgical critically ill patients is negatively correlated with APACHE Ⅱ SCOre-APACHE Ⅲ score and days of ICU.

Objective To find a new algorithm for oscillometric blood pressure measurement. Method A coefficient difference comparative method was proposed to measure the difference of adjoining pulse waves and their comparative ratios. And the turning point was judged by priority way in the range. Result The new method settled the problem of miscarriage of justice of the turning point around average pressure and improved the accuracy of blood pressure measurement. Conclusion It can detect difference between cardiovascular patients and normal persons. And it is effective and reliable in blood pressure measurement. It provides a convenient method for researching, preventing and epidemiological studies of cardiovascular diseases in our country.

80%). The resulting bulk cultures were mainly comprised of a CD56~- subset of ??T cells. The expression of V?1, V?2 and V?3 gene families in the ex vivo multiplied ??T cells from TIL and PBMC of patients with NPC, and PBMC of normal control was demonstrated by RT-PCR. Conclusion The ??TIL of NPC can be multiplied in vitro for the first time. The subsets (V?1, V?2 and V?3) of ??T cells from PBMC of healthy individuals, PBMC and TIL of patients with NPC, can also be multiplied in vitro, and the experiment lays an experimental foundation of using ??T cells for the cellular adoptive therapy in patients with NPC.

Objective Study the special value of the brainstem auditory evoked potential(BAEP)and Brain Geography Diagram(BEAM/EEG)for diagnosis of Newborn HypoxicIschemic Encephalopathy(HIE),the correlation between BAEP,BEAM/EEG and HIG,and the relation with HIE linger effect,so as to evaluate clinical curative effect,find out the best treatment time,lower the occurrence of linger effect.Methods the BEAM/EEG and BAEP were detected synehronously by adopting 16 traots of BEAM/EEG and BAEP instruments for HIE before treatment,at thirtieth day,the third Month,the sixth Month,the twelfth Month and the twenty-fourth Month respectively.Results The BAEP,BEAM/EEG have special diagnostic value on nerve cell function recovery,examination of recovery period in particalar.The research discovers the both are not synehronous in recovery period,the BAEP recovers is later than BEAM/EEG;for heavy degree HIE,BAEP recovering is slow with bad therapy effect,BAEP index sign is more sensitive to BEAM/EEG to assess linger effect.Conclusions Simple BEAM/EEG damaye indicates the brain cortex iniury only,so the prognosis is good;while BAEP damage suggestes the patiemts have brain stem nerve cell injury simultaneously,with bad prognosis for the heavy degree abnormality.in combination with BAEP and BEAM/EEG in clinical practiae,investigation of recovery degree of HIE herve cells by kinds of electric physiology angles it would have important value to perfect the treatment of HIE.