3.Clinical application of nuclear medicine imaging in amyotrophic lateral sclerosis
Peng FU ; Lingge WEI ; Ainan GUO
Chinese Journal of Nuclear Medicine and Molecular Imaging 2014;34(3):253-256
Amyotrophic lateral sclerosis (ALS),a neurodegenerative disorder,is subtle in disease onset,difficult to diagnose,and fast-growing at progression.There.are at present little specific neurological imaging characteristics of ALS.Functional brain imaging techniques,such as SPECT and PET,were the early tools for investigation of ALS.The application of pixel-based analysis software (such as SPM) renders research in this area more convenient and objective.In recent years,improvement in brain receptor PET imaging has demonstrated its important value in the early diagnosis of ALS and evaluation of cognitive impairment.
4.Alzheimer's disease and sleep disorders
Rongyan ZHU ; Peng GUO ; Wei ZHANG
Chinese Journal of Geriatrics 2017;36(1):1-4
6.Study on efficacy of Chinese herbal drugs on improving endometrial receptivity detected by using trans-vaginal three-dimensional color Doppler ultrasound in patients with thin endometrium
Hong HU ; Xiuying YE ; Yiman FU ; Wei WEI ; Peng GUO
Chongqing Medicine 2017;46(19):2635-2637
Objective To explore the clinical value of transvaginal three-dimensional color Doppler ultrasound in assessing effects of Chinese herbal drugs on improving endometrial receptivity in patients with thin endometrium.Methods A total of 76 cases of patients diagnosed with thin endometrium in Jiangbei District Hospital of Traditional Chinese Medicine from June 2015 to October 2016 were selected and treated with Chinese herbal prescription for three months.The endometrial thickness,endometrial volume and blood flow indexes before and after treatment were measured by using transvaginal three-dimensional color Doppler ultrasound.Results After 3 months treatment,the endometrial thickness and endometrial volume were increased,and the pulse index (PI) and resistance index (RI) of endometrial blood flow were decreased,compared with those before treatment,there were statistically significant differences(P<0.05).The results of three-dimensional color Doppler ultrasound indicates that after 3 months treatment the vascularization index (VI),flow index (FI) and vascularization flow index (VFI) were significantly higher than those before treatment,there were statistically significant differences (P<0.05).Conclusion The Chinese herbal prescription could increase the thickness and volume of endometrium,augment blood supply,and improve endometrial receptivity significantly.Transvaginal color three-dimensional Doppler ultrasound is a convenient and objective method for evaluating clinical efficacy of Chinese herbal prescription in patients with thin endometrium,which has high practical value.
7.The optimal power of experimental endoscopic ultrasonography-guided pancreas radiofrequency ablation therapy
Wei XU ; Yan LIU ; Peng PAN ; Yan GUO ; Zhaoshen LI
Chinese Journal of Digestive Endoscopy 2014;31(3):152-154
Objective To explore the optimal power of experimental pancreatic endoscopic ultrasonography (EUS)-guided radiofrequency ablation (RFA) therapy.Methods Six healthy live minipigs were randomly divided into two groups according to random number table,which received RFA under EUS or in laparotomy.Each pig in each group was randomly allocated to received RFA at different power (5 W,10 W,15 W).The impedance was monitored and the time for each therapy was recorded.The width diameter of coagulation necrosis from RFA was measured by EUS and gross pathology.Results RFA was completed in all pigs smoothly with stable life signs and without complications.Gross and micro pathologic observation confirmed formation of coagulation necrosis lesions.The width diameter of lesions from EUS-RFA was similar to that from surgery RFA,both having a maximum value at 10 W power (11 nun VS 10 mm).Conclusion The optimal power of experimental pancreatic EUS-RFA in pig is 10W,which should be further explored for the clinical purpose.
8.Effect of angiotensinⅡon prostatic cell proliferation and apoptosis in rats
Jing PENG ; Qian ZHANG ; Wei YU ; Yinglu GUO ; Jie JIN
Chinese Journal of Urology 2001;0(09):-
0.05). Conclusions The expression of angiotensinⅡincreases in BPH tissues in rats. It is suggested that angiotensinⅡmay affect cell proliferation but not significantly affect cell apoptosis of the rat prostate.
9.Establishment of a specific gene diagnosis method for Yersinia pestis by quantitative real-time PCR
Shanshan DONG ; Ying GUO ; Hongli TAN ; Wei LI ; Peng WANG
Chinese Journal of Endemiology 2016;35(2):119-122
Objective Using quantitative real-time PCR to establish a rapid specific genetic diagnostic technique for Yersinia pestis.Methods ①Four sets of specific probes and primers were designed,which targeted to chromosome genes of YPO0392,YPO1094,YPO2087 and YPO2090,respectively.②The probes and primers were tested for stability and specificity with 40 strains of Yersinia pestis and 47 strains of non-Yersinia pestis of different sources in Yunnan.③Eight positive DNA in Yulong,Yunnan,were tested with the screened probes and primers.Results ①Two sets probes and primers were selected,they were targeting YPO0392 and YPO1094,respectively.②The results were all positive of the eight positive DNA samples tested.Conclusion Two sets of primers and probes are selected for rapid specific diagnosis of Yersinia pestis.
10.Distribution characteristics of rs10975521 A/T and rs1929992 A/G polymorphism of IL-33 gene in Guangxi population
Jing GUO ; Yang XIANG ; Youfan PENG ; Yesheng WEI ; Yan LAN
Chinese Journal of Immunology 2016;32(6):849-852
Objective: To study and explore the distribution characteristics of rs10975521A/T and rs1929992A/G polymorphism of the IL-33 gene in Chinese Guangxi population. To compare the frequency distribution differences of allele and genotype of two sites among different ethnic. Methods:The polymorphism of rs10975521A/T and rs1929992A/G of IL-33 gene in 283 subjects were analyzed with the methods of Single base extension (PCR-SEB) and DNA sequencing,and the distribution frequency and the differences between groups of that were analyzed statisticaly. Results:Three genotypes of AA,AT and TT were found in rs10975521A/T with the frequency distribution of 12. 7%,53. 0% and 34. 3% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population ( P>0. 05 ) . There were significant differences of the allele frequency of rs10975521A/T in the Guangxi population compared with that in the European (P< 0. 05), han Chinese in Bejing (P< 0. 05) and Japanese people (P< 0. 01). Three genotypes of AA,AG and GG were found in rs1929992A/G with the frequency distribution of 23. 9%,53. 7% and 13. 4% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population(P> 0. 05),but the differences of genotype frequency of rs1929992A/G was statistically significant compared with that in the European,han Chinese in Bejing and Japanese people ( P< 0. 05 ) . There were significant differences of the allele frequency between Guangxi population and European (P< 0. 01),han Chinese in Bejing(P< 0. 05). Conclusion: There are different degrees of discrepancy of rs10975521A/T and rs1929992A/G polymorphism of IL-33 gene among different race and region.