1.Changes of psychological state and family relationship in process of treatment for depression and anxiety
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(02):-
Objective To investigate the changes of psychological state and family environment of the patients with neurosis (including depression and anxiety) in the process of treatment. Methods The Hamilton depression scale (HAMD), Hamilton anxiety scale(HAMA), self-rating depression scale(SDS), self-rating anxiety scale(SAS), family environment scale(FES), and family adaptability and cohesion scale(FACESⅡ-CV) were used to evaluate the changes of psychological state and family relationship in the process of treatment among patients with depression and anxiety hospitalized in general hospitals (n=45) and their family members (n=45). Results Before intervention, values of HAMD, HAMA, SDS and SAS in patients were higher than normal ones, and were in line with the corresponding diagnostic criteria. After psychological intervention and medication for 2 to 3 months, symptoms such as depression and anxiety were improved significantly, and values of the above scales were within normal ranges. Besides, there was also significant improvement in FES and FACESⅡ-CV after intervention among the patients. All the values of the family members were in the normal ranges before intervention, however, significantly positive changes were observed after intervention. Conclusion With the improvement in depression and anxiety after intervention among patients, the psychological pressure of family members is released, and family relationship is partially restored. The rehabilitation of patients plays an important role in the restoration of family function.
3.Minimally invasive laparoscopic-assisted surgery of the thyroid nodule
Fu-jun, XU ; Wei, CHEN ; Lei, XU
Chinese Journal of Endemiology 2009;28(3):345-346
Objective To study the feasibility, superiority and experience of minimally invasive laparoscopic-assisted surgery of the thyroid using ultrosonic scalpel. Methods In a period from January, 2006-August, 2008, 56 patients of the department of general surgery, the hospital of Heilongjiang Province were diagnosed as thyroid node. The size of the nodes ranged from 5.0 cm×4.2 cm to 3.5 cm×2.0 cm in uhrosonography. An incision of about 1.5-2.0 cm was made 1.0 cm above the suprasternal notch and the operation was performed with ultrosonic scalpel assisted with laparoscope. Partial thyroideetomy of lateral thyroid was performed in 20 cases, subtotal thyroidectomy in 25 cases, subtotal thyroidectomy of unilateral thyroid and partial thyroidectomy of contralateral thyroid respectively in 1 case, partial thyroidectomy of bilateral thyroid in 10 cases. Results All 56 cases have undergone minimally invasive laparoscopic-assisted surgery successfully in 50-146 min and no complications occurred. Fifty five cases were confirmed to be benign lesion pathologically. Conclusion Laparoscopic-assisted surgery of the thyroid is an effective with a good cosmetic result.
4.GENE EXPRESSION OF p38 MITOGEN-ACTIVATED PROTEIN KINASE AND ITS MAPKKs IN HYPERTROPHIC SCARS
Wei CHEN ; Xiaobing FU ; Xiaoqing SUN
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
To explore the change in gene expressions of p38 mitogen-activated protein kinase (p38MAPK) and its upstream signal-regulated molecule (mkk3 and mkk6) in normal skin versus hypertrophic scars underlying its potentially biological significance. Total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skin, then they were purified to mRNAs, and the gene expression of mkk3, mkk6 and p38MAPK was examined with reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the intensities of gene expression of mkk6 and p38MAPK were weak in normal skins, while the expression of these 2 genes was significantly elevated in hypertrophic scars compared with normal skins (P0.05). In hypertrophic scar, the elevation of mkk6 and p38MAPK gene expression, which plays pilot roles in cell proliferation, may be one of the mechanisms controlling the formation of hypertrophic scars.
5.EFFECT OF FGF-10 ON THE SECRETION OF GM-CSF BY NORMAL ADULT HUMAN KERATINOCYTES IN CULTURE
Xiaobing FU ; Xiaoqing SUN ; Wei CHEN
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
To investigate the effect of FGF-10 on the secretion of GM-CSF by adult keratinocytes in vitro and to understand the mechanisms involved in the stimulation of granulation tissue formation by FGF-10 during wound healing. Concentrations of FGF-10 used were 4, 16, 125 and 500 ng/ml. Cells were seeded in the amount of 2 500 cells/cm 2 or 5 000 cells/cm 2 in dishes in serum-free medium, and supernatants were collected at 24, 48 and 72 hours after culture. The amounts of GM-CSF in cell culture supernatants were determined using GM-CSF ELISA kits, and cell numbers were counted by haemocytometer. For cells seeded in low density (2 500 cells/cm 2), GM-CSF was not detected at 24 hours. At 48 hours, both in absolute concentrations and on a per-cell basis, the amounts of GM-CSF secreted in cultures with 125 and 500ng/ml FGF-10 were significantly higher than that in negative control (P0.05). At 48 hours, the keratinocytes in the middle area were confluent, and a number of cornified cells were observed, while the productions of GM-CSF in FGF-10 cultures were not higher than that in negative control. There was a clear negative correlation between the secretion production of the growth factor and the total cell number in each dish with a correlated coeffecient of 0.881 (P
6.Expression characteristics of phosphorylated forms of mitogen-activated protein kinases in hypertrophic scars
Wei CHEN ; Xiaobing FU ; Tongzhu SUN
Medical Journal of Chinese People's Liberation Army 1983;0(02):-
Objective To study expression intensity and distribution of phosphorylated forms of extracellular signal-regulated protein kinase1/2 (ERK1/2), stress-activated protein kinase (SAPK) and P38MAPK in normal skin and hypertrophic scars and their potential biological significance. Methods The morphological characteristics of hypertrophic scars in different periods after wound healing and normal skin were examined histopathologically. Protein expression of p-ERK1/2, p-SAPK and p-P38MAPK was also assessed with immunohistochemical technique. Results In normal skin, phosphorylated forms of ERK1/2, SAPK and P38MAPK were mainly located in epidermal basal cells, in which the positive cellular rates of all the three proteins were low. Along with the maturation of hypertrophic scars, protein contents of p-ERK1/2 and p-SAPK were progressively increased. In mature hypertrophic scars, positive signals of these two proteins were mostly distributed in keratinocytes and some fibroblasts. The positive cellular rate of p-P38MAPK ascended in active hypertrophic scar, then decreased to a level which was still higher than that of normal skin. Conclusion The formation of hypertrophic scars may be associated with the alteration in signaling pathways which results in the increment of protein contents of p-ERK1/2, p-SAPK and p-P38MAPK.
7.Gene expression of epidermal growth factor, c-fos and c-myc in fetal and children skins
Wei CHEN ; Xiaobing FU ; Shili GE
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To investigate gene expression of epidermal growth factor (EGF), its receptor (EGFR) and two protooncogenes (c-fos and c-myc), in fetal skin at different development stages and children skin and to delineate their potential biological significance. Methods Fetal skin biopsies of human embryo were obtained from spontaneous abortions at different gestational ages from 13 to 32 weeks and children skin specimens were collected from patients undergoing plastic surgery. After morphological characteristics of skin at different development stages were detected with histological methods, gene expressions of EGF, EGFR, c-fos and c-myc in skin at different development stages were examined with reverse transcription-polymerase chain reaction analysis (RT-PCR). Results Gene expression of EGF, EGFR, c-fos or c-myc could all be detected in fetal and childern skins. In fetal skin, the gene expression of these 4 genes was weak. Gene expression of these genes in skin was progressively enhanced with increasing gestational age. In children skin, the mRNA contents of these 4 genes were significantly increased in comparison with those in fetal skin (P
8.Expression patterns and significance of different genes in developing stage of human fetal skin
Gang ZHOU ; Xiaobing FU ; Wei CHEN
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To comprehensively analyse the transcriptional changes of genes and their biologic significance that occurred in the process of development of human fetal skin by using high-density oligonucleotide DNA array. Methods Samples of human fetal skin were obtained from aborted fetuese of 10W, 15W, 24W, 32W EGA (estimated gestational age) respectively. Total RNAs were isolated from of skin specimens of fetuses of different EGA, and mRNAs were purified and labeled with incorporation of fluorescent dUTP to prepare the hybridization probes by using reverse transcription polymerase chain reaction (RT-PCR). Approximately 21 329 human genes were spotted on a chemical-material-coated-glass plate in array. Results According to the hybridization results from oligonucleotide DNA microarray, gene expresion patterns and functions were analysed. Gene-chip disclosed a large scale of information in developmental human fetal skin, rendering a convenient way to investigate the temporal and spatial expressions of gene profile among skin cells. Many specific genes transcription expressed differently at different stages of development of fetal skin, suggesting their key roles in development, differentiation and regulation. Conclusions Microarray or DNA chip technology has revolutioned biological research by empowering to broaden the scope of collecting genomic information. Therefore, microarray-based study is able to reveal a substantial number of genes which might participate in embryogenesis and development of human skin. The present study demonstrated a previously unrecongnized role of gene expression in the control of human fetal skin growth and structure during its developmental stage. A complicated network of skin development process was fairly well characterized.
9.Effects of serum from burned rats on the gene expression in rat bone mesenchymal stem cells
Xiaobing FU ; Bing HAN ; Wei CHEN
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To investigate the effects of serum from burned rats on the gene expression in cultured rat mesenchymal stem cells (MSCs). Methods Bone marrow was extracted from sacrificed Wistar rats, and MSCs were then incubated for 24h in F-12 medium in the presence of normal rat serum (group N) or serum obtained from burned rats 3 days after injury (group B). Total RNA was extracted from both groups.The mRNA was isolated.An Oligo microarray containing 5705 genes was used to compare the differences of gene expression between two groups. Results There were four genes which expressed differently in two groups.In comparison with group N, the expression of steroid sensitive gene 1 was decreased, but that of fibroblast growth factor-4, dihydroxyacetonephosphate acyltransferase and a EST, which was moderately similar to Bmp2-inducible kinase, were increased in group B. Conclusion Serum from burned rats was able to change the gene expression of MSCs, and it might play the key role in wound repair.
10.Gene expression of mitogen-activated protein kinases in fetal skin at different developmental stages and its potentially biological siginificance
Wei CHEN ; Xiaobing FU ; Shili GE
Medical Journal of Chinese People's Liberation Army 2001;0(08):-
Objective To explore the change in gene expression of extracellular signal regulated protein kinase1 (erk1),erk2,p38MAPK and 3 c-Jun N- terminal kinases (jnk1,jnk2,jnk3) in fetal skin at different developmental stages and children skin. Methods After morphological characteristics of fetal skin at different developmental stages were examined histologically,gene expressions of erk1,erk2,p38MAPK,jnk1,jnk2 and jnk3 were determined with reverse transcription-polymerase chain reaction analysis (RT-PCR). Results Compared with early gestational fetal skin,the levels of gene expression of erk1 and erk2 showed no substantial change in late gestational fetal skin,while the contents of transcripts of p38MAPK and jnk1 were significantly decreased,the expressions of mRNA of jnk2 and jnk3 were obviously elevated. In children skin,gene expressions of erk2,p38MAPK and jnk1 were even more remarkably lowered. In contrary,gene expressions of jnk2 and jnk3 were marked enhanced. Conclusion The relative elevation of gene transcription of erk2 and p38MAPK and the inhibition of gene expression of jnk2 and jnk3 in fetal skin of earlier developmental stage might be related to fetal scarless healing.