1.Professor and National Physician Master Guo Chengjie's Asarum Clinical Application Experience
Journal of Zhejiang Chinese Medical University 2016;40(3):194-195,199
Objective]To summarize professor and national physician master Guo Chengjie asarum clinical application experience, to do good to the practitioners. [Methods] Through analyzing Guo Lao clinical experience of the medicinal nature of the composition and toxicity of understanding, to adapt to the conditions and the usage and dosage of the original understanding, analyse and summarize the experience of clinical application of asarum. [Results] Professor Guo Chengjie applies asarum poliomyelitis to cold organs and blockage for the treatment of diseases such as lung;Drug compatibility exalts development, and expands the application in the mammary gland disease by asarum;On the dosage of asarum characteristics and decoction methods give unique insights;Guo Lao especially emphasizes the importance of dialectical medication. [Conclusion] Pro. Guo's application experience and innovation, asarum clinical curative effect is good, is worth using for reference.
2.Expression of Oct-4 in gastric carcinoma and its clinical significance
Wei, QIN ; Xiao-wei, ZHANG ; Li, ZHANG ; Feng-chun, ZHANG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(6):733-736
Objective To investigate the expression of Oct-4 as a stem cell marker in gastric carcinoma tissues and its clinical significance. Methods From June 1996 to March 2006, 63 paraffin samples of gastric carcinoma tissues were obtained, and the expression of Oct-4 was examined by immunohistochemical staining. Another 58 normal tissues adjacent to gastric carcinoma and 10 normal gastric mucosa tissues were served as controls. The clinieopathologieal data of 63 patients with gastric carcinoma were retrospectively analysed, and their relationship with the expression of Oct-4 was analysed. Fifty of these 63 patients were followed up for 8 years, Kaplan-Meier method and Log-rank teat were employed to explore the correlation between Oct-4 expression and survival, and Cox Regression analysis was performed to evaluate the possibility of Oct-4 expression as an independent prognostic factor for gastric carcinoma. Results The positive expression rate of Oct-4 in gastric carcinoma tissues was 80.95% (51/63), significantly higher than that of normal tissues adjacent to gastric carcinoma (5.2%, 3/58) and normal gastric mucosa tissues (0) (P < 0.01). The expression of Oct-4 was positively correlated to the clinical stage of gastric carcinoma(P<0.01). There was no significant difference in the expression of Oct-4 among patients with different differentiation, age, and gender (P>0.05). Patients with higher expression of Oct-4 had significantly shorter survival time (P<0.05). The expression of Oct-4 was not significantly correlated with the prognosis of gastric carcinoma(RR, 0.554; 95% CI, 0.209-1.466). Conclusion The expression of Oct-4 is correlated with the clinical stage and survival time of patients with gastric carcinoma, however, it is not an independent prognostic factor for gastric carcinoma.
4.Clinical study on the effects of Buyang-Huanwu decoction combined with western medicine on early renal injury of patients with type 2 diabetes
Ke JIANG ; Runzhou ZHANG ; Wei FENG
International Journal of Traditional Chinese Medicine 2012;34(8):683-685
Objective To investigate the effects of Buyang-Huanwu decoction combined with western medicine on early diabetic renal injury.Methods 200 cases of early diabetic renal injury were randomly recruited into study group and control group,with 100 cases in each group.The control group was treated with insulin injection and oral benazepril hydrochloride,and the blood pressure,blood lipid changes were closely monitored; while the study group was additionally treated with Buyang-Huanwu decoction on the basis of the control group.Results The total therapeutic effects in the study group was significantly higher than the control group (x2=15.62,P<0.01).The values of FBG,2 h PG,DBP,SBP,HbAlc in both groups were significantly reduced after the treatment (P<0.05,t=4.23,4.47,3.59,3.87,3.99 in the study group,t=4.34,4.76,4.05,3.78,3.69 in the control group) ; but the difference between the two groups had no significance (P>0.05).Before treatment rnALB,Urea,Cr,TG and TC were not significantly different (P>0.05) ; the values of mALB,Ureaand Cr were significantly reduced in the study group after the treatment (P<0.05,t=4.56,3.73,3.28);especially mALB in the study group was obviously lower than the control group (P<0.05,t=5.01).Conclusion Buyang-Huanwu decoction combined with western medicine was effective to treat early renal damage of type 2 diabetes,with the functions of reducing urinary protein,improving microcirculation and protecting renal function.
5.Hand-washing among Medical Staff:Current Situation and Its Methods and Monitoring Measure
Runxiang ZHANG ; Wei FENG ; Jianqiu XIONG
Chinese Journal of Nosocomiology 2004;0(10):-
OBJECTIVE To investigate the present situation of hand-washing among medical staff then explore more suitable hand-washing methods and monitoring measure. METHODS By surveying the hand-washing situation of 96 medical staff in our hospital through the method of sampling,the different germ eradication rate between routine soap hand-washing and disinfectant wet towel hand-wiping as well as hand-washing rate in all candidate population during practical work were observed. RESULTS The germ eradication rate after routine soap hand-washing was 86.0%,while by disinfectant wet towel wiping it was increased to 91.3%.The washing rate after above former method was 18.3% and after above two methods combined was increased to 43.6%,and after health propaganding and training was up to 60.6%. CONCLUSIONS Disinfectant wet towel hand-wiping is convenient,practical and effective which would be more suitable for clinical practice when combined with routine soap washing method.
6.Laparoscopic cholecystectomy in aged patients:Report of 2335 cases
Zhenbo ZHANG ; Fuquan YANG ; Wei FENG
Chinese Journal of Minimally Invasive Surgery 2001;0(01):-
Objective To study the value of laparoscopic cholecystectomy (LC) for treating gallbladder benign diseases in aged patients. Methods Laparoscopy cholecystectomy (3-port or 4-port) was performed in 2335 senile patients with gallbladder benign diseases from December 1992 to May 2005. Results Five patients were found as having the Mirizzi syndrome and were given a subtotal cholecystectomy. In 18 patients with a frozen Callot’s triangle, the anterior wall of the gallbladder was resected and the mucous membrane on the posterior wall was electrocauterized. LC was completed smoothly in the remaining 2312 patients. No conversions to open surgery were needed. The operation time was 8~55 min (mean, 19 min), and the intraoperative blood loss was 1~ 50 ml (mean, 8 ml). An abdominal drainage tube was placed in 213 patients, including double-cannula lavage in 56 patients. The length of hospital stay was 4~16 d (mean, 6 d). Follow-up examinations for 10 days ~13 years in 1859 patients showed no biliary stenosis, gallbladder pouch, or residual stones. Conclusions Laparoscopic cholecystectomy is safe and feasible in aged patients, being the first choice of operative method. Proper perioperative management, careful prevention co-morbidities, and control of operation time as short as possible are important for senile patients.
7.Estrogen reduces apoptosis induced by H_2O_2 in PC 12 cells
Mei ZHANG ; Wei LI ; Jianqiang FENG
Basic & Clinical Medicine 2006;0(08):-
Objective To observe the effect of estrogen on H2O2-induced apoptosis and to explore the mechanism of protection by estrogen.Methods Setting up the experimental model of apoptosis induced by H2O2 in PC12 cells. The viability of cells was assessed by MTT assay. The activity of lactate dehydrogenase was detected by colorimetry. Hoechst 33258 was used as indicator of apoptosis. Apoptotic cells were measured by flow cytometry (FCM) with propidium iodide stain. The activity of caspase-3 was detected by colorimetry. Results H2O2 significantly decreased the viability of cell, increased LDH release and promoted apoptosis as well as caspase-3 activity in PC12. Estrogen markedly reduced these changes. Conclusion Estrogen reduces apoptosis induced by H2O2, and its mechanisms may be explained by inhibition of activation of caspase-3.
8.Research Progress of Small Non-coding RNA in Bacteria
Wei ZHANG ; Yigang TONG ; Fumin FENG
Microbiology 2008;0(07):-
Small non-coding RNA (sRNA) is a kind of newly discovered 50 nt~500 nt small RNAs that do not encode proteins. To date, more than 150 sRNA have been found in bacteria. The small RNA acting by base-pairing with target mRNAs, resulting in post-transcriptionally regulating gene expression, is important regulators in the bacterial response to stress, virulence and metabolism. At present, researches of sRNA mainly based on bioinformatical prediction and molecular biological experiments. The sRNA that obtained through these methods needs confirmation in laboratory, and then study of its functions through a variety of experimental methods.
9.Analysis of cell parameter in peripheral blood of occupational n-hexane exposed workers.
Feng ZHU ; Wei-sen ZHANG ; Hui ZUO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2007;25(7):402-403
Adult
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Female
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Hematologic Tests
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Hexanes
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adverse effects
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Humans
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Male
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Occupational Exposure
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adverse effects
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Young Adult
10.Clinical evaluation of maxillary sinus elevation without osteotome from the top of alveolar ridge.
Wei GAO ; Liang-yu LI ; Feng ZHANG
Chinese Journal of Stomatology 2013;48(3):183-185
Adult
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Aged
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Alveolar Ridge Augmentation
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methods
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Dental Implantation
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methods
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Female
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Humans
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Male
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Maxillary Sinus
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surgery
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Middle Aged