Quantitative proteomics is a novel subject of proteomics research. There are several new techniques employed for the protein quantitative study. Isobaric tags for relative and absolute quantitation (iTRAQ) technology for protein quantitation using mass spectrometry is a recent powerful means of determining relative and absolute protein levels in up to four samples simultaneously. The iTRAQ reagent produced high quality, reproducible result in enriched complexes, organelles, and whole cell lysates. The status of the recent promising techniques and their possible future evolution were reviewed.

Objective To investigate the effect of CD34+ stem cells seeded stent graft in venous system. Method Twelve mongrel dogs were randomized into 2 groups. Stent grafts covered with PTFE or Dacron were implanted into infrarenal vena cava through a delivery system. In each group, 4 stent grafts were seeded by autogenous bone marrow CD34+ cells, and 2 treated by autogenous blood only as control. The grafts were explanted at 2 weeks. Light and electron microscopy were used to examine endothelialization of neointima. CD34 and Ⅷ factor stain were applied to identify endothelial cells. Results All stent grafts were patent except for one Dacron stent without seeding. Light, electron microscopy, CD34 and Ⅷ factor stain demonstrated that confluent endothelial cells appeared on the neointima of seeded grafts. No endothelial cells were detected in the non-seeding grafts. Conclusions Rapid endothelialization of stent grafts can be achieved through bone marrow CD34+ cells seeding in venous systems.

Cholangitis, Sclerosing ; etiology ; Genetic Predisposition to Disease ; Hepatitis, Autoimmune ; etiology ; Humans ; Liver Cirrhosis, Biliary ; etiology

Adult ; Arthritis ; diagnosis ; diagnostic imaging ; Female ; Humans ; Radiography ; Tuberculosis, Lymph Node ; diagnosis ; diagnostic imaging ; Tuberculosis, Osteoarticular ; diagnosis ; diagnostic imaging ; Ultrasonography

Hepatocytes ; cytology ; immunology ; transplantation ; Humans ; Liver Failure ; therapy ; Living Donors ; Tissue Donors

Objective: To determine the noninvasive imaging efficacy of 99mTc-sandostatin scintigraphy for lung cancer. Methods: 57 consecutive patients with pulmonary nodules(PN) were studied with 99mTc-sandostatin scintigraphy.Planar imaging was obtained after injection of (991.6?187.59) MBq of 99mTc-sandostatin at 1.5-4 hour with GE Dual-head gamma camera(Millennium VG, Hawkeye ;General Electric Medical Systems) . SPECT images of the chest were performed at 4-6 h post injection. All scintigraphically detected lesions were confirmed by histopathological analysis and/or by other imaging modalities.Tumor to normal tissues ratios (T/N) were calculated . Results: Out of 57 patients ,47 were malignant tumors; 12 with small cell lung cancer (SCLC), 35 with non- small cell lung cancer (NSCLC). 10 had benign lesions. The sensitivity , specificity and accuracy of 99mTc-Sandostatin in detection of lung cancer were 95.7%, 90%, 94.7%, respectively. In two patients (pts) with pulmonary squamous cell cancer 99mTc-Sandostatin imaging was false negative. In 10 pts. with benign PN, 9 pts. was true negative, but one patient with tubercolama was false positive. T/N ratio was 3.43?0.66, 2.24?0.31 in SCLC and NSCLC respectively. The T/N ratio was higher in small cell lung cancer than NSCLC(t = 4.072 ,P

Ascites ; diagnosis ; etiology ; Diagnosis, Differential ; Humans ; Liver Cirrhosis ; complications ; diagnosis

Dopamine ; therapeutic use ; Hepatorenal Syndrome ; blood ; therapy ; Humans ; Liver Transplantation ; Vasoconstrictor Agents ; therapeutic use

Cell Differentiation ; Gene Expression Regulation ; Liver ; cytology ; Stem Cells ; cytology

Adenoviridae ; genetics ; Animals ; Cell Transplantation ; methods ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Graft Rejection ; prevention & control ; Hepatocytes ; immunology ; transplantation ; Humans ; Liver ; metabolism ; Liver Failure ; surgery ; Simian virus 40 ; genetics ; Transfection ; Transplantation, Homologous