Objective:To investigate the correlation of FAT10 expression with the malignant characteristics of hepatocellular car-cinoma (HCC), and to explore the effect of FAT10 on RhoA and cytoskeleton of HCC. Methods: Immunohistochemistry (IHC) was used to detect the FAT10 expression level of 108 HCC patients, and the correlation between the expression of FAT10 and the malignant characteristics of HCC patients was analyzed. We transiently transfected plasmids with overexpressed FAT10 using 7721 and HepG2 cells or interfered with FAT10 expression using siRNA in Huh7 and LM3 cells. Active-RhoA, total-RhoA, and ROCK protein expres-sion levels were detected by Western blot analysis after overexpression or interference. We also used immunofluorescence to detect changes in the cytoskeleton protein F-actin after FAT10 overexpression in 7721 cells. Results:Correlation analysis showed that both ac-tive-RhoA and FAT10 expression levels were significantly correlated with clinical malignant characteristics by using IHC (RhoA:me-tastasis, P=0.036 and recurrence, P=0.026;FAT10:metastasis, P=0.031 and recurrence P=0.004). In addition, active-RhoA expression level was correlated with FAT10 (P=0.000). Survival analysis showed that the prognoses of low-expression active RhoA (P=0.019) or FAT10 (P=0.026) groups were significantly better than those of the high-expression groups. Western blot analysis showed that FAT10 increased the expression of active-RhoA and ROCK. However, the expression of active-RhoA and ROCK decreased after FAT10 inter-ference. F-actin expression increased in the 7721 cells with overexpressed FAT10 (all P<0.01). Moreover, FAT10 facilitated F-actin ag-gregation on cell membrane and changes in F-actin. Conclusion:FAT10 is correlated with the malignant characteristics of HCC and may promote changes in HCC cytoskeleton induced by active-RhoA.

After the institutional scientific research management at present was analyzed according to the literature investigation, expert consultation, comparative and comprehensive analysis, the following were advanced, including the principles, target, function module and structure module for institutional scientific research management infor-mation system , and realization of its functions using the J2 EE technology-based B/S framework .

Objective To explore the digital morphological and chromatic characteristics of erythrocytes infected with Plasmodium vivax. Methods The images of both normal and P. vivax infected erythrocytes were segmented and measured for morphologic parameters including area, length, breadth, perimeter, roundness, aspect ratio, equivalent circle diameter, as well as chromatic parameters including saturation and color (red, green, blue). A statistic analysis was performed for these parameters. Results Both morphological and chromatic parameters showed high significant differences between the normal and the infected erythrocytes, and high significant differences between the normal and the erythrocytes infected with different stages of P. vivax. Conclusion The differences mentioned above could be used as the basis for automatic identification of P.vivax in thin peripheral blood smears.

Objective:To investigate the correlation between MRI features of breast cancer and the expression of CBL-B, AXL and ITGB2, and further to provide new imaging ideas for the early diagnosis of breast cancer, the designation of treatment plan and the preoperative non-invasive assessment of breast cancer prognosis.Methods:204 female patients with breast cancer who visited Linyi Central Hospital from Sep. 2017 to Sep. 2020 and received surgical treatment were selected as research objects. A total of 204 lesions were selected. Scanning was performed using Philips All-digital Ingenia3.0T MRI. Tumor diameter, shape, edge, intensity pattern, time-signal intensity curve (TIC) type, early intensity rate and ADC value were recorded. Immunohistochemical SP method was used to detect the expression levels of CBL-B, AXL and ITGB2 in breast cancer tissues and adjacent tissues.Results:The proportion of patients with low expression of CBL-B, high expression of AXL and high expression of ITGB2 in breast cancer tissues was significantly higher than that in adjacent tissues (All P<0.05) . Univariate analysis showed that tumor diameter, shape, early enhancement rate and ADC value in MRI features were correlated with the expression of CBL-B protein in breast cancer (All P<0.05) . The tumor diameter, shape and TIC typing in MRI features were associated with AXL protein expression in breast cancer. The tumor diameter, TIC typing, early enhancement rate and ADC value in MRI features were related to ITGB2 protein expression. Binary Logistic regression analysis showed that tumor diameter ≥3 cm and early enhancement rate ≥100% were independent risk factors for low expression of CBL-B in breast cancer ( P<0.05) . Tumor diameter ≥3 cm, and irregular shape or burr were independent risk factors for AXL high expression ( P<0.05) . Tumor diameter ≥3 cm, early enhancement rate ≥100%, ADC value ≥1.12×10 -3 mm 2/s were independent risk factors for high ITGB2 expression in breast cancer ( P<0.05) . Conclusion:MRI features of breast cancer can be used to predict the expression of CBL-B, AXL, and ITGB2, so as to provide reference for clinical diagnosis, treatment and prognosis assessment of the disease.

Objective To investigate clinical service delivered at the Polyclinic of Beijing 2008 Olympic and Paralympic Village and outcomes of the medical patients visited there.to provide experiences in medical care service for other mass-gatherings in the future.Methads Clinical data of all the medical patients evaluated at the Polyclinic during July 27 to August 27 and during August 29 to September 19,2008 were reviewed retrospectively.with univariate and multivariate analyses.Results In total.1537 and 1206 medical patients visited the Polyclinic at the Olympic and Paralympic Village,respectively.Proportion of athletes visited was hisher during the Paralympic Games than that during the Olympic Games(14.2% vs.9.8%,P<0.01).We reeeived 775 patients(50.4%)with acute respiratory tract disorders,including 16 cases(1.0%)with asthma,during the Olympic Games,and 686(56.9%),including eight cases (0.7%)with asthma,during the Paralympic Games,and antibiotics were prescribed for 149(9.7%)and162(13.4%)of them,respectively.Twelve(0.8%)patients were hospitalized during the Olympic Games and four(0.3%)during the Paralyrnpie Games,and age equal to or nlore than 55 years and digestive disease were independent risk factors for hospitalization,with odds ratios(ORs) of 5.3 and 9.5 and their 95% confidence intervals(Cls)of 1.4-19.6 and 2.5-36.4,respectively.Conclusions Acute respiratory disorder was the most connnon cause for medical visits in the Polyclinic.Asthma did not play a significant role to iniluence athletes'performance in the Beijing 2008 Olympic and Paralympic Games.Antibiotic prescription in the Polyclinic had been controled to an acceptable level.Conditions in elder patients and those with digestive disorder tended to be severe.deserving more attention infuture's mass gathering.

Objective To evaluate prescribing antibiotics in medical patients visiting the polyclinic at 2008 Beijing Olympic Village for its further reasonable use. Methods Clinical prescriptions for all the medical patients at the polyclinic were reviewed retrospectively. Consistency between antibiotics prescriptions and their indications formulated before 2008 Beijing Olympic Games was evaluated, as well as related factors for their overuse. Results In total, 1537 patients visited the polyclinic and 1233 recipes were prescribed,with 956 patients (62. 2% ) with infections, including 809 (52. 6% ) of respiratory infections. One hundred and forty-nine of 1537 patients (9. 7% ) were treated with antibiotics, accounting for 12. 1 percent of all prescriptions, and 43 cases (28.9%) were identified as antibiotics overuse, including 39 cases with respiratory infections. Prevalence of antibiotics overuse was significantly more for patients of domestic employees than that for non-employees (34. 6% vs. 14.3% , P <0. 01 ), with art independent association between them (OR 3.07, 95% CI 1.11 - 8. 50). Conclusions Although the polyclinic has successfully reduced unnecessary antibiotics use, their overuse remains to be reduced, and physicians' strict adhesion to indications for antibiotics use and patient education are essential for their rational use in primary care of China.

After the development of medical and health think tanks in China and foreign countries were compara-tively analyzed, the problems of their development in China were pointed out, including a fewer number of inde-pendent think tanks and independently selected studies, and less international influencing power, with measures for the development of medical and health think tanks in China put forward, such as paying attention to their competi-tiveness, intensifying their support strength, and optimizing the employed.

Objective To investigate the effect of liver detoxification soup combined with compound glycyrrhizin on hepatitis-related factors in patients with chronic hepatitis B.MethodsFrom April 2014 to August 2015, 86 patients with chronic hepatitis B were enrolled in this study.Random number table was divided into observation group and control group (43 cases).The control group was given routine glycyrrhizin and other conventional (ALT), serum total bilirubin (TBil), serum albumin transferase (ALT) and serum total bilirubin (TBil) were measured before and after treatment.(HA), laminin (LN), type Ⅳ collagen (Ⅳ-C) and type Ⅲ procollagen (PCⅢ) were used to analyze the effect of hepatic fibrosis, And observe adverse reactions.ResultsThe effective rate of the observation group was 93.02% higher than that of the control group (76.74%, P<0.05).After treatment, the patients were treated with chest discomfort, emotional depression, epigastric fullness, fatigue, ALT (35.28±1.35) U/L, HA (165.25±1.44) mg/L, and the treatment group (P<0.05).After treatment, the levels of TBil (30.34±1.59)μmol/L, Alb (48.32±1.33)g/L, and the level of serum was significantly higher than that of the control group (P<0.01).The incidence of adverse reactions was 11.63% and 6.98% in the two groups.ConclusionTraditional Chinese medicine hepatectomy decoction combined with compound glycyrrhizin can effectively treat chronic hepatitis B and improve the level of serum hepatitis-related factors, and the adverse reaction is light, it is worthy of clinical application.

[Objective] To observe the preventive effect of Huanglian Jiedu Decoction (HJD), a prescription with the actions of clearing heat and removing toxicity, on rabbits atherosclerosis (AS) induced by Chlamydia pneumoniae (CP) infection. [Methods] Fifty two New Zealand rabbits were fed with forage containing 2. 5g/kg cholesterol and were infected with CP via nasopharynx for three times during 6 weeks. At the end of the sixth week, forty four rabbits with serum CP-IgG positive were randomized into 4 groups: group A treated with HJD 2g?kg-1 ?d-1 by gastric gavage, group B with HJD 1g?kg-1?d-1, group C with azithromycin 20 mg?kg-1?d-1 and model group D with normal saline for 6 weeks. Group E was set up in 8 rabbits fed with fatty forage and served as the control. Serum levels of high-sensitive C reactive protein (hs-CRP) and CP-IgG were detected before infection, after infection and after treatment. At the end of the 18th week, the rabbits were executed and the pathological features of aortic tissue were observed under microscope. Meanwhile, the expression of CP-DNA and tumor necrosis factor ?(TNF-?) in the aortic tissue was detected with polymerase chain reaction (PCR) and immunohistochemical method respectively. [Results] At the end of the experiment, atherosclerotic changes were obvious in groups D and E, particularly in group D. Compared with group D, the atherosclerotic damage was much relieved, and AS indexes such as maximum intimal thickness (MIT), atherosclerotic damage percentage and plaque area index were much improved in groups A and C (P

Immunoglobulins ( Ig ) , also called antibodies, are important components in humoral -mediated immunity. Ig can bind with their receptors, called immunoglobulin receptors ( IgR ) , trigger biologic activities respectively. Different sub-types of Igs show different function. And IgRs have been treated as therapeutic targets in inflammation and immunity related dis-eases for many years. This article reviewed the recent progresses in the study of IgR function and its therapeutic role in inflamma-tion and immunity related diseases.