1.Paneth cell-rich carcinoma of stomach: report of two cases.
Wei-dong SHI ; Chun-nian HE ; Jin-ping ZHAI ; Jin-hai SUN ; Chen CHEN
Chinese Journal of Pathology 2006;35(2):123-124
Adenocarcinoma
;
pathology
;
surgery
;
Aged
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Carcinoma, Signet Ring Cell
;
pathology
;
surgery
;
Cell Differentiation
;
Female
;
Follow-Up Studies
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Gastrectomy
;
methods
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Humans
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Male
;
Middle Aged
;
Paneth Cells
;
pathology
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Stomach Neoplasms
;
pathology
;
surgery
2.Goblet cell carcinoid of appendix: report of two cases.
Xue-dong ZHANG ; Chun-nian HE ; Jin-ping ZHAI ; Huan-fen ZHAO ; Chen CHEN ; Wei-dong SHI
Chinese Journal of Pathology 2006;35(2):126-127
Adenocarcinoma, Mucinous
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pathology
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Aged
;
Appendectomy
;
methods
;
Appendiceal Neoplasms
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pathology
;
surgery
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Appendicitis
;
pathology
;
Appendix
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pathology
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Carcinoid Tumor
;
pathology
;
surgery
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Diagnosis, Differential
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Female
;
Follow-Up Studies
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Humans
;
Male
3.Effect of Tripterygium wilfordii polyglycosidium on caspase expression of islet?-cell in NOD mice
Li-Ping GU ; Yi-Jie WU ; Yu-Fei WANG ; Nian-Wei SHI ; Wei-Ping DONG ; Jian LIN
Chinese Journal of Endocrinology and Metabolism 1985;0(02):-
NOD mice were treated intraperitoneally with tripterygium wilfordii ployglycosidium (TWP) for 4 weeks to observe the incidence of cyclophosphamide accelerated diabetes.The apoptosis of?-cells was detected by TUNEL,the expression of caspase-3 in islets of the NOD mice was detected by immunohistochemistry and the expression of caspase-8 mRNA in pancreas by RT-PCR.The results revealed that the incidence of diabetes in TWP group was lower than that in control group.The apoptosis index of?-cells was decreased in TWP group. The expression of caspase-3 in islets and the expression of caspase-8 mRNA in pancreas in TWP group were lower than those in control group.
4.Clinical effects of the circumcision stapler, foreskin cerclage, and traditional circumcision: A comparative study.
Hui-dong MIAO ; Jia-wei LU ; Fu-nian LU ; Feng SHEN ; Xiao-lin YUAN ; Hai-yong LIU
National Journal of Andrology 2015;21(4):334-337
OBJECTIVETo compare the clinical effects of the circumcision stapler, circumcision cerclage, and traditional circumcision in the treatment of phimosis and redundant prepuce.
METHODSUsing the circumcision stapler (group A), foreskin cerclage (group B), and traditional circumcision (group C), we treated 276 patients with phimosis or redundant prepuce. We made comparisons among the three groups in the operation time, intraoperative blood loss, intraoperative and 24-hour postoperative pain scores, and incidence of postoperative complications. Results: The operation time, intraoperative blood loss, and intraoperative pain score were (6.52 ± 2.45) min, (1.93 ± 0.82) ml, and 1.37 ± 0.68 in group A and (7.24 ± 1.86) min, (1.51 ± 0.72) ml, and 1.20 ± 0.79 in group B, all significantly lower than (28. 36 ± 4.22) min, (9.52 ± 3.29) ml, and 3.06 ± 0.75 in group C (P <0.05). The 24-hour postoperative pain score was remarkably higher in group B than in A and C (3. 18 ± 0. 82 vs 1. 85 ± 0. 63 and 1. 82 ± 0. 75, P <0. 05). The incidence rate of postoperative complications was markedly lower in group A than in B (5. 43% vs 14. 13%, P < 0.05), but with no significant differences between either A and C or B and C (P >0.05).
CONCLUSIONThe circumcision stapler, with its advantages of simple operation, minimal invasiveness, fewer complications, and better cosmetic result, deserves a wider clinical application.
Blood Loss, Surgical ; Circumcision, Male ; adverse effects ; instrumentation ; methods ; Foreskin ; Humans ; Incidence ; Male ; Pain Measurement ; Pain, Postoperative ; diagnosis ; Penis ; abnormalities ; Phimosis ; therapy ; Postoperative Complications ; Postoperative Period
5.Primary Hodgkin lymphoma of the intestinal tract: report of a case.
Wei-Dong SHI ; Chun-Nian HE ; Huan-Fen ZHAO ; Ping LI ; Chen CHEN ; Xiu-Zhi ZHANG ; Shu-Yan ZHANG
Chinese Journal of Pathology 2008;37(7):493-494
Aged
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Female
;
Hodgkin Disease
;
diagnosis
;
pathology
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Humans
;
Reed-Sternberg Cells
;
pathology
6.Optimization of method for determination of Salviae Miltiorrhizae Radix et Rhizoma.
Nian-jun YU ; Dai-yin PENG ; Wei-dong CHEN ; Hua-sheng PENG ; Fan YU ; Shun-min SHI
China Journal of Chinese Materia Medica 2015;40(11):2128-2131
OBJECTIVETo optimize the method in the Chinese Pharmacopoeia for determining Salviae Miltiorrhizae Radix et Rhizoma.
METHODTanshinone II(A) and salvianolic acid B were selected as the index in optimization of the sample preparation method of Salviae Miltiorrhizae Radix et Rhizoma in Chinese Pharmacopoeia. Orthogonal test was used to optimize the extraction process of Salviae Miltiorrhizae Radix et Rhizoma, and concentration of contents were detected by high performance liquid chromatography method. A detection of using methanol-water (85: 15) at wavelength of 270 nm was employed for tanshinone II(A) and a detection of using methanol-acetonitrile-formic acid-water (30:10:1: 59) at wavelength of 286 nm was employed for salvianolic acid B.
RESULTThe optimized extraction process of tanshinone II(A) and salvianolic acid B was: extracted by 90% methanol and reflux twice (0.5 h each time) at 75 degrees C, extracted by 70% methanol and reflux twice (1.5 h each time) at 75 degrees C, respectively.
CONCLUSIONOptimized extraction and determination methods could be used to reflect the content of tanshinone II(A) and salvianolic acid B in Salviae Miltiorrhizae Radix et Rhizoma more accurately and efficiently.
Benzofurans ; analysis ; Chromatography, High Pressure Liquid ; Diterpenes, Abietane ; analysis ; Rhizome ; chemistry ; Salvia miltiorrhiza ; chemistry ; Temperature
7.Genetic research of pulmonary atresia
Li GONG ; Jia-Wei SHI ; Nian-Guo DONG
Chinese Journal of Applied Clinical Pediatrics 2013;28(1):29-31
Objective To explore the genetic defects in patients with pulmonary atresia (PA).Methods Twenty-three patients with PA were studied from recent 1 year.There were 15 boys and 8 girls,aged 2 days to 10 years.Among them,there were 3 cases with complete ventricle septum,20 cases with VSD,3 cases without main pulmonary trunk,20 cases with PDA,6 cases with integrated major aorto-pulmonary collateral arteries(MAPCAs),3 cases with integrated right aortic arch,2 cases with integrated extra-cardiac abnormality with the manifestation of facial abnormality,1 case with integrated aberrant right subclavian artery,and 1 case with integrated tracheal bronchus.The chromosomes of the children were routinely analyzed.Negative chromosomes were examined to identify the 22ql 1.2 microdeletion by fluorescence in situ hybridization (FISH) test.Results One case of trisomy syndrome was found.22ql 1.2 microdeletion was found in 3 cases.Among 22q1 1 microdeletion cases,1 case was intact ventricular septum,2 cases were integrated VSD.22q1 1.2 microdeletion was not found in the rest 19 cases.Conclusions Twenty-one trisomy syndrome and Del 22q1 1.2 syndrome may play an important role in the pathogenesis of PA.TBX1 probe can be used to examine 22q1 1.2 microdeletion.Del 22q1 1.2 syndrome was suspected in cases of incorporating aberrant subclavian artery,MAPCAs and severe poor development of pulmonary artery.
8.Application of Endoscopic Vein Harvesting in Obese Patients Undergoing Coronary Artery Bypass Grafting
Peng BAI ; Yi-Xuan WANG ; Si CHEN ; Jin-Ping LIU ; Nian-Guo DONG ; Jun-Wei LIU
Journal of Huazhong University of Science and Technology (Medical Sciences) 2018;38(4):691-696
This study aims to evaluate the clinical outcomes of endoscopic vein harvesting (EVH) for coronary artery bypass grafting (CABG) in obese patients.Totally,153 obese patients who underwent EVH (n=81) or standard bridging technique (SBT,n=72) in CABG surgery from May 2012 to October 2014 in our hospital were enrolled in this retrospective non-randomized controlled study.The general situation of operation,postoperative complications and short medium-term outcomes were analyzed.The baseline characteristics were similar between these two groups (P>0.05).There were no statistical differences in total operation time (226±28 min vs.224±30 min,P>0.05),number of damaged vessels (0.12±0.05 vs.0.16±0.06,P>0.05) and short medium-term outcomes including revascularization rate (1.25% vs.2.78%,P>0.05),vessel dysfunction rate (11.25% vs.11.11%,P>0.05) and mortality (0.00% vs.0.00%,P>0.05).Use of EVH was associated with significant reduction of total harvesting time (41±6 min vs.63±11min,P<0.05),incision length (4.4±1.1 cm vs.18.2±4.5 cm,P<0.05) and postoperative lower extremity complications (P<0.05).EVH can reduce the risk of wound complications,whereas does not influence short-and medium-term outcomes in obese patients.It can be considered a reliable procedure of harvesting vessel conduits for obese patients undergoing CABG.
9.Effects of Malassezia isolates on cytokines production associated with melanogenesis by keratinocytes.
Fan CUI ; Xiao-Dong SHE ; Xiao-Fang LI ; Yong-Nian SHEN ; Gui-Xia LÜ ; Wei-Da LIU
Acta Academiae Medicinae Sinicae 2007;29(2):196-200
OBJECTIVETo investigate the co-culture of keratinocytes with Malassezia isolates which cause the pityriasis versicolor with different color and to analyze the changes of cytokines associated with melanogenesis.
METHODSThe effects of Malassezia species with different proportions on the growth rate of keratinocytes was assessed with 5 g/L methyl thiazolyl tetrazolium (MTT). Co-culture of keratinocytes and Malassezia species were performed with isolates from hyer- and hypo-pigmentation areas of pityriasis versicolor. The supernatants were collected at different time points, and the changes of basic fibroblast growth factor (b-FGF), endothelin-1 (ET-1), nerve growth factor-beta (NGF-beta), interleukin-1alpha (IL-1alpha), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), stem cell factor (SCF) were recorded. Three control groups were established accordingly.
RESULTSWhen the ratio between keratinocytes and Malassezia species was lower than 1: 10, the growth rate of keratinocytes was not affected by Malassezia (P > 0.05). When the ratio was increased above 1:20, the growth rate of keratinocytes was significantly inhibited by Malassezia (P < 0.01). The secretions of IL-1alpha, IL-6, TNF-alpha, and ET-1 was significantly increased after the co-culture of keratinocytes and Malassezia (P < 0.01), while those of b-FGF, NGF-beta, and SCF had no significant changes (P > 0.05). Compared with the isolates from the hypo-pigmentation area, ET-1 induced by isolate from hyperpigmentation area significantly increased (P < 0.01).
CONCLUSIONWhen Malassezia isolates are co-cultured with keratinocytes, the secretions of cytokines associated with melanogenesis may differ from each other. ET-1 may play certain role in the hyper-pigmentation of pityriasis versicolor.
Cell Proliferation ; Cells, Cultured ; Cytokines ; biosynthesis ; Humans ; Keratinocytes ; cytology ; metabolism ; microbiology ; Malassezia ; isolation & purification ; physiology ; Melanins ; biosynthesis ; Tinea Versicolor ; microbiology
10.Model of B immunoblastic lymphomas in the Hu-PBL-SCID mice.
Dong-qing CHEN ; Lian-jun BAI ; Qing-feng LIU ; Lian-xian CUI ; Wei HE ; De-nian BA
Acta Academiae Medicinae Sinicae 2003;25(3):294-296
OBJECTIVETo constitute a model of B immunoblastic lymphomas in the Hu-PBL-SCID mice.
METHODSThe SCID mice were reconstituted by intraperitoneal injection (i.p.) of 5 x 10(7) human lymphocytes from Epstein-Barr virus (EBV) seronegative individuals. After one week, the SCID mice were inoculated with EBV by i.p. injection, and subjected to the investigation of whether there was any tumor in the abdomen of such SCID mice four weeks later. The characteristics of the found tumor was observed by the methods of Hematoxylin-eosin (HE) stain, immunohistochemical staining and polymerase chain reaction (PCR).
RESULTSCompared with the control groups, all the EBV-infected Hu-PBL-SCID mice had abdominal solid tumors [(32 +/- 12.5) mm3] developed, often located in the liver. HE staining and immunohistochemical staining showed the tumors were human B cell lymphomas. EBV DNA could be detected in the tumors by the PCR.
CONCLUSIONSThe model of B immunoblastic lymphomas in the Hu-PBL-SCID mice is successfully constituted, and may well be useful to the human tumor immunological study.
Animals ; Disease Models, Animal ; Herpesvirus 4, Human ; physiology ; Humans ; Lymphoma, Large-Cell, Immunoblastic ; Mice ; Mice, SCID