Many details concerning the interventional diagnosis and therapy of thyroid disease need to be studied deeply and carefully; for example, the thyroid applied anatomy, especially the anatomical imageology study of the thyroid arterial anastomosis involving the therapeutic effects and complications, the thyroid artery embolization treatment, presently as a substitution or the supplement therapy for the traditional classical therapy of Graves disease. There are many exploration to extend the indication scope, the therapeutic effect, embolization scope in intervention for Graves disease due to having no accurate individual quantification standard, and so on. The thyroid arterial embolization for Graves disease is to reduce the main cause of thyroxin secretion. Simultaneously, the comprehensive therapeutic effects might have possibly produce the thyroidcytic apoptosis and immune adjustments. Serious complications such as the cerebral infarction, the hyperthyroidism crisis, the hypocalcemia, the periodic paralysis, and so on commonly occured in thyroid arterial embolization. It is necessary to keep strictly the procedure rule and the indication. The mid-and long-term therapeutic effect of thyroid artery embolization for Graves disease is good, but still need more extensive and prudent research for the prospective achievement.

Objective The study is mainly on the effect and outcome of 131 I treatment for children and adolescents with differentiated thyroid cancer .Methods We compared the difference between rhTSH -aided ra-dioiodine treatment and L -thyroxin withdrawal .Our study collected 87 patients in a retrospective study .In order to analysis the effect,we divided into two groups.The experimental group has 46 patients,and control check has 41 patients.We compared the basic characteristics and mortality in different groups by χ2 -test,t-test and F-test based on data type .Results The thyroid stimulating hormone concentrations of experimental group patients have significant differences in the first ,the third and the sixth day after the injected of rhTSH ,the third day is the highest.The TSH concentration has significant difference between the two groups before 131I treatment(t=2.362, P=0.023).For the Tg(thyrobolulin)negative patients,Tg concentration in control check is higher than the third day after injected of rhTSH in the experiment group (1.5 ±1.2 vs.0.7 ±1.4,P=0.034).Whole body image has been done by scintigraphic after 3~8 months of 131I treatment.Thirty four(84%)patients have no radioiodine up-take in experiment group,and 40(87%)patients have no radioiodine uptake in control check ,there is no differ-ence between the two groups (χ2 =0.277,P=0.599).There is no difference between the two groups on the rea-son for retreatment(P=0.875).Conclusion The outcome of rhTSH -aided radioiodine treatment is similar to L-thyroxin withdrawal in short -term recurrences ,and rhTSH-aided radioiodine treatment with 131 I therapy for children and adolescents with DTC is a better method .

Animals ; Cells, Cultured ; Female ; Granulosa Cells ; metabolism ; Pituitary Adenylate Cyclase-Activating Polypeptide ; genetics ; metabolism ; Platelet Activating Factor ; pharmacology ; RNA, Messenger ; genetics ; Rats ; Rats, Sprague-Dawley

Objective To investigate the changes of the levels of high sensitivity reactive protein C (hsCRP), white blood cell (WBC) and fibrinogen (FIB) in type 2 diabetic patients with metabolic syndrom (MS). Method 323 inpatients with type 2 diabetes were divided into MS group and non-MS group according to their clinical dates, and the changes of the levels of hsCRP, WBC and FIB in the two groups and the relationship between their levels and MS were analyzed. Results Compared to non-MS group, the levels of hsCRP, WBC, FIB in MS group were increased obviously (P

OBJECTIVE To evaluate the application of antibiotics and its trend for reference to clinical utilization.METHODS The data about antibiotics used from 2005 to 2007 were collected and analyzed.RESULTS The consumption of antibiotics was fallen year by year.The rate of utilization was 67%.The rate of drug combination was 45% and the rate of antibiotic resistance test was 20%.CONCLUSIONS There are still a lot of problems in antibiotics usage of our hospital.It is necessary to strengthen the guidance and supervision.

OBJECTIVEThis study measures the glutaredoxin (Grx) gene and protein expression in umbilical vein endothelial cells upon exposure to Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS). The involvement of the Akt-signaling pathway is also determined.

METHODSEA-hy926 cells were pretreated with 1,000 ng · mL⁻¹ P. gingivalis LPS for 4, 12, 18, and 24 h, and then real-time reverse transcription polymerase chain reaction was employed to detect Grx1 expression. The effect of Grx on Akt activity was investigated using Western blot for the control, LPS (1,000 ng · mL⁻¹ LPS), and carmus- tine (BCNU) groups (1,000 ng · mL⁻¹ LPS, and the EA-hy926 cells were pretreated with 25 μmol · ml⁻¹ BCNU for 30 min).

RESULTSGene expression of Grx1 significantly increased in LPS group compared with that in the control group. The Grx1 expression reached the peak level in 12 h, and the variation between the expression in 4 and 12 h was significant (P < 0.05). After 12 h, the protein levels of Grx and phosphorylated-Akt (p-Akt) significantly increased in the LPS group (P < 0.05), whereas the BCNU group showed a considerable decrease in both Grx and p-Akt expression levels (P < 0.05). Moreover, a slight difference was observed in the total Akt protein levels in the three groups (P > 0.05).

CONCLUSIONGrx expression increased upon exposure of EA-hy926 cells to the LPS. Akt activity could be inhibited by BCNU (a Grx inhibitor), which indicated that Akt might act as a downstream regulator of Grx.

Endothelial Cells ; Glutaredoxins ; genetics ; Humans ; Lipopolysaccharides ; pharmacology ; Oxidative Stress ; drug effects ; Phosphorylation ; Porphyromonas gingivalis ; pathogenicity ; Proto-Oncogene Proteins c-akt ; drug effects ; Signal Transduction ; drug effects ; Umbilical Veins

Objectives To evaluate the effect of control measures and provide the basis for development of preventive measures though dynamical monitoring of the operation of water improvement projects and know The prevalent trends of endemic fluorosis.Methods In accordance with the provisions and requirements of the Surveillance Scheme of Drinking-Water-Borne Endemic Fluorosis(Trial),136 monitoring counties were selected in national 27 provinces(autonomous regions,municipalities) and Xin Jiang Production and Construction Corps,and 10 water improvement projects were selected in each county.The operation of water improvement projects was investigated,and water fluoride content was tested.Three epidemic villages were selected as fixed monitoring villages in each county.In the villages,the fluoride content in drinking water was detected by Standard Testing Method of Drinking Water.Dental fluorosis was diagnosed by Dean's method of all 8 to 12-year-old students in the villages.Stratified random sampling by age was used to select 50 adults over 25-year-old in each monitoring village,and skeletal fluorosis was examined by X-ray according to the Diagnosis Standard of Endemic Skeletal Fluorosis.Results ①A total of 1398 water improvement projects were monitored.Normal and intermittent operation and discarded projects accounted for 93.35％(1305/1398),5.29％(74/1398) and 1.36％(19/1398),respectively.The qualification rate of water fluoride content was 69.96％ (978/1398).② In the 327 surveyed villages with water improvement projects,the qualification rate of water fluoride content of the projects accounted for 76.15％(249/327).In the 84 surveyed villages without water improvement projects,villages of water fluoride ＞ 1.2 mg/L and ≤2.0 mg/L,＞ 2.0 mg/L,and ≤4.0 mg/L,and ＞ 4.0 mg/L,accounted for 57.14％ (48/84),32.14％(27/84) and 10.71％(9/84),respectively.③ In the 195 villages with water improvement projects and the projects were in normal operation with qualified water,the detection rate of dental fluorosis and the defect type of dental fluorosis in children were 25.03％ (3851/15 387) and 1.88％ (289/15 387),respectively,and for the 82 villages with fluoride content exceeded the national standard,the two rates were 56.27％ (3384/6014) and 10.92％(657/6014),respectively.④ In the 195 villages with water improvement projects and the projects were in normal operation with qualified water,the detection rate of X-ray skeletal fluorosis was 19.11％ (1465/7667).In the 79 villages with fluoride content exceeded the national standard,the detection rate of X-ray skeletal fluorosis was 17.47％ (634/3630).Conclusions The operation of water improvement projects and water quality need to be further improved,and the measure of changing water plays a certain role in control of drinking-water-borne endemic fluorosis,which needs a long-term consolidation.

Objective In order to provide the basis for establishment of food allergy models , we compared the differences of sensitivity and alterations of intestinal flora of food allergy models in two strains of mice .Methods Forty 4-5-week old female BALB/c and Kunmimg mice were divided into control group ( n=10) and food allergy goup ( n=30), respectively.Ovalbumin (OVA) was injected to the mice to establish food allergy models .Serum OVA-specific IgE of the mice was assayed by ELISA .The jejunum tissue was examined by pathology with HE staining .The changes of fecal flora were detected by denaturing gradient gel electrophoresis (DGGE).Results (1)Among the sensitized 60 mice, OVA-sIgE levels were significantly increased in 27/30 BALB/c mice and 21/30 KM mice compared with those of control groups(P<0.001).Moreover, there were more evident inflammatory cell infiltration , epithelial cell shedding and cytolysis in the jejunal villi of BALB/c mice than those of KM mice.(2) After food allergy modeling, there were significant changes of intestinal flora in the BALB/c mice (P<0.001), while only significant change of evenness was found in the KM mice (P<0.05).(3)There were changes of abundance , Shannon index and evenness of intestinal flora in the model groups of BALB/c and KM mice.Conclusions BALB/c mice are more sensitive to OVA allergy than KM mice .The composition of intestinal flora is different among different strains of mice .The changes of intestinal flora after OVA challenge in BALB /c mice are more obvious than those in KM mice .

Objective To explore the clinical value of hs-CTnT among acute myocardial infarction patients.Methods 60 acute myocardial infarction patients were treated from July 2014 to October 2016 in our hospital, set as the observation group where venous blood samples were collected on admission, 4 hours after admission, 12 hours after admission and the control group (60 health human where the venous blood samples were collected in the morning), respectively.The hs-CTnT, myohemoglobin, creatine kinase isoenzyme and tropnin T were recorded for analysis.Results On arrival of hospital, the positive rate of CK-MBmass, Myo, hs-cTnT, cTnT was 75.0%、71.7%, 90.8%, 80.0%, respectively.Hs-cTnT positive rate raised to 100.0% and CK-MBmass (83.3%), Myo (75.0%), cTnT (88.3%) after 4 hours.Besides, after hospitalization, the hs-cTnT positive rates raised up.The rates apparently increased at different times (P<0.05).The positive predictive value was 90.0% and sensibility 91.0%, which was significantly higher than other indicators (P<0.05).Conclusion The high sensitivity and specify of hs-cTnT indicator level could be one of the early diagnosis index for acute myocardial infarction.

OBJECTIVE To investigate the pathogens in the early period after heart transplantation and analyze their drug-resistance.METHODS The pathogens in the early period after heart transplantation were identified and their drug-resistance was analyzed.RESULTS From all of the 121 pathogens,the rate of G-bacilli was 73.6%,the rate of G+ cocci was 17.4%and the rate of fungi was 9.1%;G-bacilli mainly consisted of Klebsiella pneumoniae,Enterobacter cloacae,Acinetobacter baumannii,Pseudomonas aeruginosa and Escherichia coli.G-bacilli showed higher sensitive rates to sulbactam/cefoperazone,cefepime,piperacillin/tazobactam and ceftazidime than to cefotaxime.All G-bacilli showed sensitive to imipenem except Pseudomonas aeruginosa.G+ cocci mainly consisted of negative coagulase Staphylococcus,Staphylococcus aureus and Enterococcus.Fungi mainly consisted of Candida,and they were sensitive to fluconazole,itraconazole and amphotericin B.CONCLUSIONS To observe the pathogens in the early period after heart transplantation and analyze their drug-resistance are important to control and prevent the infection efficiently for the heart transplantation recipients.