Diabetes is a common disease,serverely harmful to the human's health and life quality.The pancreatic islets transplantation can correct the patient's hyperglycemia,stop or even reverse the progress of the complication and thus decrease the mortality of diabetic patients.It is the most safe and efficient therapy for diabetes.Since the Edmonton Protocol got success in pancreatic islet transplantation in 2000,it has been more and more interested because of its great clinical curative effect.Research strategy of islet transplantation is now focussed on increasing the acquired islets with normal viability,selecting the best transplantation pathway,and improving the immunosuppression protocol.The shortage of human pancreatic donor is an ever unsolved problem in clinical application.The potential resolutions may include acquisition from xenogenic-islets;islets originated from stem cells,and islets from the living-donor human pancreas.The islets transplantation will open a new application field for interventional radiology.(J Intervent Radiol,2006,15: 577-579)

This paper analyzes the newest CT and MR products and compares their new technology and clinical application.The development of image inosculation technology is also briefly discussed.

Objective To study the clinical efficacy of island skin flap in the repair of skin and subcutaneous soft tissue defects caused by excision of malignant tumour in the skin. Methods Island skin flaps with the main artery and its penetrating branches were used to repair the skin and soft tissue defects in different sites after malignant tumour removal. A total of 5 cases were repaired, in which 2 cases were in the face, 2 in limb and one in wall of the chest. Results Among the 5 cases, one was repaired with island skin flaps with the main artery, and another 4 were repaired with island skin flaps with the penetrating arteries. Follow-up for one to 5 years showed that the appearance and function in the repaired sites were normal without blood flow disturbance and dysfunction of the island skin flap, and no recurrence of the tumors except one case. Conclusion The island skin flap with main artery, especially one with its penetrating branches, is a good option to repair the defect caused by removal of malignant tumors with high rate of success, better appearance and minor damage to the donor site.

Objective To study the operative methods and clinical outcome of laparoscopic ileal colpopoiesis with long vascular pedicle.Methods We reviewed 31 cases which had received ileal colpopoiesis,including 25 cases of congenital absence of vagina and 6 cases of male-to-female transsex.All cases were operated on by laparoscopic(8 cases) and laparoscopy assisted(23 cases) ileal colpopoiesis.The postoperative results were analyzed through physical examination and interview regarding the patient′s functional status and satisfaction during sexual intercourse.Results Ileal colpopoiesis were preformed successfully on all 31 cases.Follow-ups of 3-13 months were done for all of them.Patients used vaginal mould for at least 8 months.All artificial vaginas had excellent cosmetic results,including adequate vaginal length,good lubrication,and appearance and physical functions similar to female natural vagina,and sexual function was reportedly satisfactory.One of the patients had an operative complication of an intestinal obstruction,which was cured by a second operation and other treatments.Conclusions The laparoscopic and laparoscopy assisted ileal vaginoplasty is a feasible,safe and ideal method of current vaginoplasty due to its minor damage,excellent cosmetic results and similarity to normal vaginal features.However,vaginal mould should be used for a long period of time(at least 8 months) after an operation,to prevent vaginal orifice shrinkage.

Objective To investigate the stress response during cardiopulmonary bypass and the effects of propofol on it Methods Thirty one patients scheduled for valve replacement ,were divided randomly into propofol group (group A,n=16) and control group (group B, n=15) Anesthesia was induced with valium fentanyl vencuronium, was maintained with fentanyl enflurane vencuronium In group A propofol 6 8mg?kg -1 ?h -1 was infused intravenously during CPB MAP,venous plasma concentrations of epinephrine(E) ,norepinephrine(NE) and blood glucose were measured before CPB(T1),cooling to 33℃(T2) and 30℃(T3), stable hypothemia(T4), rewarming to 30℃(T5) and 33℃(T6),and 15 min after CPB(T7) respectively Results In rewarming period, NE and E levels in group B increased significantly (P0 05) The dose of sodium nitroprussid was lower in group A than that in group B, and the dose of dopamine was not different between both groups Conclusions The stress response increases in rewarming stage during CPB ,which can be suppressed by the continuous infusion at anesthetic dose of propofol

A growing body of evidence has indicated the important role of autophagy receptors in directing ubiquitinated or non-ubiquitinated cargos towards autophagy. Autophagy receptors bind to LC3 (microtubule-associated protein 1 light chain 3) on phagophore and autophagosome membranes, and recognize signals on cargoes in the delivery system of autophagy. However, the diverse domains in the receptor structures determine that their roles would never be limited to autophagy. Up to date, increasing numbers of the receptor proteins have been demonstrated to serve as a molecular link or switch participating in autophagic degradation, apoptosis or cell survival signals. Here, we highlight the non-autophagic roles of these receptor proteins to draw attention to this growing research topic.
Apoptosis ; Autophagy ; Humans ; Microtubule-Associated Proteins ; physiology ; Signal Transduction ; Ubiquitination

The nafion/carbon nanoparticles (CNPs) composite film modified glassy carbon electrode was pre pared by dropwise method, and the electrocatalyst of H_2O_2 at Nafion/CNPs modified electrode was investiga ted.The results show that electrocataytic activitie for detection of H_2O_2 at the modified electrode is very good.The biosensing application of CNPs was demonstrated through fabrication of an electrochemical biosensor.The biosensor was constructed by encapsulating glucose oxidase in the Nafion/CNPs composite film.The biosensor had good electrocatalytic activity toward oxidation of glucose.The glucose biosensor shows a linear range from 2.0 × 10 ~(-6) to 6.0×10~(-3) mol/L with a detection limit of 1.6×10 ~(-6) mol/L.The biosensor shows high stabili ty, good reproducibility and can avoid the commonly coexisted interference.In addition, real rat serum samples were analyzed by this biosensor with satisfactory results.

Objective To investigate the clinical value and effect of peripherally inserted central eatheter(PICC) in patients with tuberculous meningitis. Methods 56 patients with tuberculous meningi-tis were randomly divided into the treatment group and the control group with 28 cases in each group. The treatment group was medicated by PICC, while the patients of the control group were medicated through pe-ripheral superficial vein. The curing rate, degree of anxiety, infusion response, satisfaction with nursing and related expense were recorded. Results The curing rate and satisfaction with nursing of the treatment group were higher than those of the control group. The anxiety degree, infusion response and related ex-pense of the treatment group were lower than those of the control group.Their difference had statistical sig-nificance. Conclusions PICC has clinical value in patients with tuberculous meningitis,which is worth promoting.

Objective To develop a kind of multi-functional cleaning-out device for medical apparatus.Methods Drawing on experience of traditional medical apparatus cleaning techniques by human or machine,a new cleaning-out device was designed.The prototype was made and proofed by series of tests.Results This device has outstanding advantages in producing cost,cleaning quality and cleaning cost.Conclusion This device worth popularizing for its easy-operation and low cost.

Objective To study the adhesive characters of Lactobacillus to enterocyte-like HT-29 cells.Methods A HT-29 cell culture was employed to investigate the adherent ability of Lactobacillus.Bacterial suspension and HT-29 cells were co-incubated at 37℃ for 1h.The cells with adherent bacteria were Gram-stained,and then examined microscopically under oil immersion.The pH of the medium,bacterial growth phase,co-incubation time,bacterial concentration,D-mannose and type of host cells,which were involved in the process of adherence of Lactobacillus to enterocyte-like HT-29 cells,were also investigated.Results The adherent level increased substantially for Lactobacillus reuteri JCM1081 as the pH decreased from 6.0 to 4.0.Maximum adherence occurred within 90min and remained stable until the end of incubation.The bacterial concentration exerted a certain influence on the adherence of Lactobacillus reuteri JCM1081 to HT-29 cells,and it seemed to be concentration dependent.Maximum adherence occurred in the concentration of 109cfu/ml and remained stable until the end of incubation.Lactobacillus strain that grew into stationary phase showed higher ability to adhere to HT-29 cells than that in logarithmic phase.The addition of D-mannose dramatically decreased the level of Lactobacillus adherence.The Lactobacillus strain adhered in higher levels to the enterocyte-like cells or gastric epithelium cells than other cell origins,and the adhesion of Lactobacillus showed host cells specificity.Conclusion The adhesive characters observed with Lactobacillus suggested that adhesive properties are species and host specific.The pH,bacterial growth phase,co-incubation time,bacterial concentration and D-mannose may influence the adhesion of Lactobacillus to enterocyte-like HT-29 cells.