Objective To explore the role of the change of the CD11b expression in hyperleukocytosis and acute promyelocytic leukemia(APL) differentiation syndrome, we observed the effects of all-trans retinoie acid(ATRA) and trioxide arsenic(ATO) alone or the combination together with daunorubicin (DNR) on CD11b expression of human APL cell line NB4 cells. Methods By using the mono-antibody of CD11b FITC-conjugated, we monitored the level of CD11b expression of NB4 cells by flow cytometry. Results With the length of the 1 μmol/L ATRA exposure,the CD11b expression was increased. Exposed to ATRA for 24 h, 48 h, 72 h and 168 h;the CD11b expression of NB4 cell was (33.34±3.15)%, (55.59±5.13)%, (86.08±5.12)% and (90.69±2.69)%, respectively which were higher than control group and the difference was statistically significant (P<0.01). With the length of the 1 μmol/L ATO exposure, the CD11b expression showed no significant difference with control group(P>0.05), but was significantly lower than that in ATRA exposure(P<0.01). Exposed to 1 μmol/L ATRA combined with 1 μmol/L ATO for 72 h and 168 h, the CD11b expression were (18.81±1.40)% and (25.61±4.54)%, which were significantly higher than that in control group (P<0.05, P<0.01), but significantly lower than ATRA exposure (P<0.01). With the exposure to 1 μmol/L ATRA combined with 1 μmol/L ATO and 1 μmol/L DNR, the CD11b expression was also significantly lower than ATRA exposure (P<0.01) and showed no difference with control group (P>0.05). Conclusion By avoiding the increase of CD11b expression of promyelocytic leukemia cells, ATRA combined with ATO or two drugs together with DNR could play a role in eliminating incidence of hyperleukocytosis and retinoic acid syndrome.

Objective:To evaluate the value of adjuvant radiotherapy for stage Ⅱ endometrial cancer after surgery.Methods:40 patients with stage Ⅱ endometrial cancer with over five years follow up were analyzed vetrosectively.27 cases received subradical hysterectomy,and additional pelvic lymph node dissection in 13 cases.6 cases were treated with surgery alone.34 cases were treated with postoperative pelvic irradiation and additional vaginal brachytherapy in 12 cases.Results:The 5 year survival rate,pelvic failure rate and extrapelvic recurrence rate were 65%(26/40),17.5%(7/40),25%(10/40) repectively.The 5 year disease free survival rate,pelvic failure rate and extrapelvic recurrence rate were 70.5%(24/34),5.9%(2/34),29%(10/34) respectively in 34 patients with postoperative radiotherapy.5 cases with surgery alone recurrenced at the vaginal residual within two years.The late complications were 44%(15/34),including proctitis,cystitis and intestinal obstruction.Conclusion:Postoperative radiotherapy for stage Ⅱ endometrial cancer can improve the tumor local control and increase the late complications.

Chromatography, Gas ; methods ; Dimethoate ; blood ; Humans ; Insecticides ; blood ; Methyl Parathion ; blood

In top-quality course construction of medical ethics,it is necessary to attach importance to practical operation as well as the consistency of theoretical logic.To well adapt to the current medical practice and medical technical advances,the idea of medical ethics should include virtue,harmony and value.Virtue is the prominent concept in top-quality course construction of medical ethics.Harmony is the core idea of medical ethics,and value is the innovative idea to solve the questions caused by life technologies.Therefore,in the top-quality course construction of medical ethics,coordination and unification of the above three ideas must be maintained in order to defend the demonstration significance of top-quality course of medical ethics.

Adolescent ; Altitude ; Anaerobic Threshold ; physiology ; Drugs, Chinese Herbal ; therapeutic use ; Emigrants and Immigrants ; Humans ; Hypoxia ; physiopathology ; prevention & control ; Male ; Oxygen Consumption ; physiology ; Physical Endurance ; physiology ; Phytotherapy ; Young Adult

Acupuncture Therapy ; Adult ; Fasciitis ; therapy ; Female ; Humans ; Male ; Scapula ; injuries ; Young Adult

0.05).Conclusions There is imbalance of Th1/Th2 cells in CHB children.Th1 cytokines are positively correlated with hepatic inflammatory activity.In the course of disease,Th2 cytokines are predominant and they have a correlation to the chronic prognosis of the illness.So we can conclude the degree and prognosis of disease by observing the change of Th1/Th2 cytokines in CHB children.

The nafion/carbon nanoparticles (CNPs) composite film modified glassy carbon electrode was pre pared by dropwise method, and the electrocatalyst of H_2O_2 at Nafion/CNPs modified electrode was investiga ted.The results show that electrocataytic activitie for detection of H_2O_2 at the modified electrode is very good.The biosensing application of CNPs was demonstrated through fabrication of an electrochemical biosensor.The biosensor was constructed by encapsulating glucose oxidase in the Nafion/CNPs composite film.The biosensor had good electrocatalytic activity toward oxidation of glucose.The glucose biosensor shows a linear range from 2.0 × 10 ~(-6) to 6.0×10~(-3) mol/L with a detection limit of 1.6×10 ~(-6) mol/L.The biosensor shows high stabili ty, good reproducibility and can avoid the commonly coexisted interference.In addition, real rat serum samples were analyzed by this biosensor with satisfactory results.

Aged, 80 and over ; Amyloid beta-Peptides ; metabolism ; Brain ; pathology ; Cerebral Amyloid Angiopathy ; complications ; metabolism ; pathology ; Cerebral Hemorrhage ; etiology ; pathology ; Consciousness Disorders ; etiology ; pathology ; Humans ; Male

0.05).Except imipenem and ampicillin,there was significant difference between the resistance of non-ESBLs-producing strains from CAP and from HAP to other eleven kinds of antibiotics(?2 test,P