Objective: To observe the atrial expression of angiotensin II receptor type 1 (AT1R) and α7-nicotinic acetylcholine receptor(α7nAChR) during the development of heart failure in rats. Methods: Male SD rats were randomly divided into 4 groups. Abdominal aorta coarctation (AAC) was used to prepare heart failure model. Expression of AT1R and α7nAChR was determined by Western blotting analysis at 4,8,12, and 16 weeks after AAC in all the groups. Results: Compared with the control group, expression of AT1R and α7nAChR in AAC group was not significantly different at all the 4 time points after AAC. Conclusion: Our findings suggest that atrial AT1R and α7nAChR may not be involved in the early stage pathological process of AAC-induced heart failure.

Decision Making, Organizational ; Disasters ; Earthquakes ; Humans ; Public Health Practice ; Stress Disorders, Post-Traumatic

Bayes Theorem ; Public Health ; Translational Medical Research

Evidence-based medicine emphasizes making project of diagnosis and therapy on the basis of the most objective research results. The current condition in which domestic medicine is dominant in hepatobiliary surgery and teaching needs to be changed urgently. The procedure of application of evidence-based medicine in teaching of hepatobiliary surgery is explained by an actual example. Evidence-based medicine plays an important role in teaching and quick progress in all of the hepatobiliary surgery.

Objective To investigate the effect of estrogen on gliosis, synaptic proliferation and reorganization in hippocampus of rats with chronic epilepsy induced by pentylenetetrazol (PTZ). Methods Thirty-two Wistar rats were randomly divided into normal control group, PTZ kindling epilepsy group, estrogen+PTZ group and estrogen +Tamoxifen (mixed estrogen antagonist)+PTZ group. The PTZ kindling epilepsy group was intraperitoneally injected with 35 mg/kg PTZ once a day, then the behavior of the rats in one hour was observed. The estrogen interfered group was intramuscluarly injected of 0.6 ?g/kg ?-estradiol 6 h before PTZ injection, 1/3 d. The estrogen+Tamoxifen interfered group was injected of Tamoxifen into lateral cerebral ventricle 1 h before estrogen injection. If the severe epileptic seizures occurred three times, the rats were ready for detecting the expression of filial fibrillary acidic protein (GFAP) and synaptophysin (P38) by immunohistochemical technique. Results Epileptic seizure occoured earlier and more severely in most rats of estrogen interfered group than no estrogen interfered group. Rats in all model groups were found more astrocyte proliferation and positive synaptophysin staining, especially in the CA2, CA3 and hilar areas of dentate gyrus (DG) of hippocampus than control group. Estrogen+Tamoxifen interfered group showed significant difference as compared with other groups (P

Objective To observe the effects of intensive insulin therapy on IL-10 level and NF-ΚB activity in peripheral blood mononuclear cells in patients undergoing cardiopulmonary bypass.Methods The non-diabetic patients undergoing cardiopulmonary bypass in our department were selected and assigned to intensive therapy group (group A,n=40) and received strict glycemic control after the initiation of surgery.And those who undergoing cardiac surgery but without strict glycemic control were assigned to routine therapy group (group B,n=40) as controls.The blood glucose in group A was maintained at 4.4～8.3mmol/L,whereas the glucose in group B was below 11.lmmol/L.The concentration of serum IL-10 and NF-ΚB activity in peripheral blood mononuclear cells was measured at different time points.Results There were no significant differences in general data between two groups.The concentration of IL-10 in group B was significantly lower than that in group A(P<0.05).compared with group B,strict glycemic control markedly suppressed NF-KB activation (P<0.05).Conclusion Intensive insulin therapy could reduce the activity of NF-ΚB and then reduce the expression of IL-10.Strict glycemic control could significantly mitigate the systemic inflammatory response.

A non spore fungal strain designated as CR 9512 was isolated from a species of Cordyceps , found in Northern Guangdong of China The growth characteristics and the morphology of the organism were studied It was initially identified as Rhizoctonia sp ,Agonomycetales Its antimicrobial tests, against 26 strains of Gram positive and Gram negative bacteria, actinomycetes, filamentous fungi and yeast, showed strong inhibition to some yeast of Rhodotorula, Saccharomyces, Pichia and Cryptococcus , and weak inhibition to yeast of Candida The speciality on a limited spectrum anti yeast of the organism will show an enormous value for researches and applications

Objective To investigate the neuroprotection of delayed treatment of thoracic acute spinal cord injury with recombinant human erythropoietin (rhEPO) in rat model of compressive injury. Methods Sixteen adult male Sprague-Dawley rats were randomly divided into two groups: control group (compressive injury group) and experimental group (rhEPO group), In the compressive injury group,the animals recived 0.9% saline treatment at 2 h, day 1 and day 3 after the injury, while in the rhEPO group, rhEPO (3 000 U/kg) was given to rats at 2 h, day 1 and day 3 after the injury. All the rats were observed in 4 days after the injury. The primary outcomes were evaluated by BBB scale, apoptotic index, inflammatory index and electron microscopy. Results Delayed treatment of thoracic acute spinal cord injury with rhEPO could reduce apoptosis, regulate inflammation, improve motor function and promote regeneration of the spinal cord. Conclusion Our study suggests that delayed treatment of thoracic spinal cord compressive injury with rhEPO could exert neuroprotection.