Hepatic fibrosis is a wound-healing response to liver injury, which has complex celluar and molecular mechanisms. Animal models that similar to human hepatic fibrosis are important to explore further pathological mechanisms and more effective drug therapy for hepatic fibrosis. This article reviews recent progress in animal models and antifibrotic therapy related to hepatic fibrosis.

Indices of angiogenesis are increased in synovia from patients with rheumatoid arthritis, and vascu-lar proliferation may contribute to the pathogenesis of synovitis, pan nus growth, bone and cartilage destruction, and osteophyte formation. Many proan giogenic factors are expressed in the synovium in RA. They have been shown to ha ve important effects in the angiogenic process in RA. So the blockade of angioge nesis appears to be a promising avenue for the future treatment of RA.

Objective:To investigate the participation of clinical pharmacists in the drug treatment regimen and the methods of pharmaceutical care. Methods:Clinical pharmacists participated in the treatment of one case of cervical malignant melanoma and pro-vided the comprehensive pharmaceutical care, including making the individualized anti-tumor treatment plan, calculating the dose, mo-nitoring the adverse drug reactions. Results: The therapeutic schedule suggested by clinical pharmacists was adopted to improve the clinical outcomes and reduce the adverse reactions. Conclusion: The participation of clinical pharmacists in the practice of clinical drug therapy can improve the level of drug therapy.

OBJECTIVE:To prepare ganciclovir dispersible tablet and to observe its dissolution property in vitro.METHODS:The dosage of microcrystalline cellulose and crospolyvinylpyrrolidone in the formula were optimized by orthogonal test;The content of ganciclovir was determined by UV spectrophotometry method;Dissolution rates were compared among ganciclovir dispersible tablet,Ganciclovir common tablet and Ganciclovir capsule.RESULTS:The optimized formula dosage of microcrystalline cellulose and crospolyvinylpyrrolidone were40%and10%respectively;The linear concentration range for ganciclovir was2～20?g/ml(r=0.9999,n=6);The average recovery was(100.21?0.38)%;The mean values of intraday and the inter-day precision were0.22%and0.32%respectively;The ganciclovir dispersible tablet could completely disinte-grated within30s,T 50 was(0.03?0.001)min and T d was(0.18?0.006)min,which were all lower than those of the common tablet and capsule(P

Objective To investigate the'expression of Survivin in breast cancer tissue microarray, and its implication to clinic pathology and prognosis. Methods The expression of Survivin was studied in 80 cases of breast disease tissues ( 18 cases of hyperplasia of mammary glands and 62 cases of breast canc-er) and 20 cases of adjacent normal tissues by S-P immunohistochemisty to analysis the relationships be-tween its expression and history grade, clinic stage and lymphaden metastasis. Results The positive rates of Survivin were 0(0/20) in normal mammary glands, 33. 3% (6/18) in hyperplasia of mammary glands and 72. 5% (45/62) in breast cancer. There were significant difference between them (X~2= 34. 62, P <0. 01 ). The expression of Survivin was closely correlated to the stage and lymphaden metatasis and survival time ( P <0.05 or P <0.01), and it was negatively correlated to the histological grade ( P >0.05).Conclusions The expression of Survivin shows an ascending tendency in the carcinogenesis and progres-sion of breast cancer. The Survivin can promote the invasion and the metastasis of the cancer cell, and it is closely correlated to the prognosis.

Objective To establish a method for determining Ganciclovir concentration in dog serum by RP-HPLC.Methods The serum was determined by high performance liquid chromatography.A C_(18) column(5 ?m,250?4.6 mm) was applied.The mobile phase was 15 mmol/L potassium phosphate buffer(pH 2.5) containing 0.25% acetonitrile.The flow rate was 1.75 ml/min.The detector set at 254 nm.Column tempetature was 40 ℃.Results The linearity was excellent over the range of 0.050 25-100.5 ?g/ml;The methodological recovery ranged from 96.565% to 105.718%.The within-day and between-days relative standard deviations(RSD) were less than 1.400% and 3.800% respectively.Conclusion The elaborated method is sensitive,precise and can be used in the pharmacokinetic studies.

Objective To analyze the clinical curative effect and the impact for denture health by using a fixed denture to repair denti-tion defect of the elderly.Methods A total of 90 elderly patients with dentition defect(118 with tooth)were selected in our hospital from January 2012 to September 2013,who were divided into control group and observation group after the relevant examination,45 cases in each group.The patients in control group were treated by removable dentures,and patients in observation group received fixed denture repair.All patients were followed up for 2 years,the health status and the denture bleeding index between two groups were compared and evaluated by patients feedback and chewing ability tests.Results The clinical total effective rate of observation group after treatment was obviously higher than that of control group,the difference was statistically significant(P <0.05).The health effective rate of abutments of the observation group after 2 years was significantly higher than that of the control group,the difference was statistically significant(P <0.05).Bleeding index of two groups after repair were significantly lower than those before,the difference was statistically significant(P <0.05),which reduced more obviously than control group,the difference was statistically significant(P <0.05).Conclusion The fixed denture has high clinical value for elderly patients with dentition defect,which can effectively repair the complex tooth and tooth defect,with good effect and high safety.

Objective To observe the effect of silencing the expression of ADM using RNA interfering technique on the expression ofβ-catenin and terminal differentiation of osteosarcoma cells.Methods After the intervention of 0 h,48 h and 72 h by ADM siRNA,we observed the change of distribution ofβ-catenin in F5M2 using immunocytochemistry staining.The expression levels of total and phosphorylatedβ-catenin and GSK3βwere detected by Western blot after the intervention by ADM siRNA.The F5 M2 cells treated with ADM siRNA were subjected to HE staining,alkaline phosphatase (ALP)assay and immunocytochemistry staining to investigate the biological effects of ADM siRNA on the morphology and terminal differentiation of F5M2 cells.Results After the intervention of 0 h,48 h and 72 h by ADM siRNA,the distribution ofβ-catenin was transferred from the cytoplasm to the nucleus.ADM siRNA downregulated the expression level of P-β-catenin and upregulated P-GSK3βdetected by Western blot.HE staining revealed that the configuration of F5M2 had undergone restorational changes similar to those of normal cells after ADM siRNA treatment.ALP assay and immunocytochemistry staining showed that the expression of the earlier and later molecular biomarkers of terminal differentiation,including ALP and osteocalcin were strongly positive.Conclusion Silencing the expression of ADM can activateβ-catenin to transfer to the nucleus from the cytoplasm,and induce osteosarcoma cells to make terminal differentiation through activatingβ-catenin signaling pathway.

Objective To investigate the profession development status of intra-veineuse (IV) nurse team in Anhui province, provide the reference and baseline data on intravenous therapy management of province unity standard. Methods Using the cluster sampling method, the questionnaire designed by IV professional committee of Anhui province was used to investigate the status of IV nurse team in 137 hospitals. Results IV nurse in 35 hospitals (25.6%) was accepted the intravenous treatment knowledge training, however no specific provision for training time in 91 hospitals (66.4%). Organization in PICC evaluation was diversified, such as manufacturer (40 accounted for 29.2%), nursing association in province (city)( 38 accounted for 27.7%) and nursing department in hospital (36 accounted for 26.3%). 28 hospitals had PIVAS (20.4%). 62 hospitals established IV nurse team (45.3%), 87 hospitals established IV quality control system (63.5%). Implementation strength was good in the special inspection, the following feedback in standardized operation and adverse events (81 accounted for 59.1%, 72 accounted for 52.6%and 75 accounted for 54.7%, respectively), however, there was no enough emphasis on regular meeting, organization consultation and collection data of regular basis (59 accounted for 43.1%, 52 accounted for 38.0%and 32 accounted for 23.4%, respectively). Conclusions IV nurse team has been preliminarily established in Anhui province, however there are some problems, for example, specialist nurse training and examination is not unified, overall quality control system have not established, external conditions need to be improved. Related departments should actively formulate corresponding countermeasure to promote the healthy development of the IV nurse team.