Objective To introduce a rapid spot test method for nitrite in water to meet the urgent need of nitrite monitoring during accidental pollution. Methods According to the drinking water legislation in Europe (threshold: 0.10 mg/L NO2-), a rapid spot test method for nitrite in water was established by using an optimized composition of reagent mixture and a binary positive/negative response, conducted as follows: a tablet of a mixture of solid reagents containing sulfanilamide, N-(1-naphthyl) ethylene diamine dihydrochloride and sodium chloride was put on a spot test plate of Teflon mould, then, a drop of 30%(V/V) HCl and a drop of water sample were added on the reagent material. Results Under these conditions, all the test samples containing ≥0.10 mg/L NO2- can produce a positive response of the characteristic red-purple color, whereas, the samples will produce a negative response when the level of NO2- was below the limit. The results of real water samples determined with this new method were confirmed by using the photometric method based on the use of the same reagents. Conclusion The method is simple, rapid , accurate, and suitable for field monitoring of nitrite in water.

The prevalence of Toxoplasma gondii infection in free-range chickens in Jilin province, northeastern China was investigated. A total of 1095 serum samples were collected from nine administrative regions from July to October, 2012, and antibodies to T. gondii were examined by an in-house enzyme-linked immunosorbent assay (ELISA) using Toxoplasma lysate antigen (TLA). The detection results were confirmed by Western blot. The overall seroprevalence of T. gondii in free-range chickens was 17.6% (95% confidence interval [CI], 15.4–19.4%), ranging from 13.3% (95% CI, 6.3–20.4%) in Siping to 23.6% (95% CI, 15.7–31.6%) in Liaoyuan. There was no significant difference in T. gondii infection among different regions in Jilin province (P> 0.05). The widespread presence of T. gondii infection in freerange chickens of Jilin province implies the wide contamination with T. gondii oocysts in the living environment of people, and free-range chickens might be an important source of infection for humans.

The design of field medicine automobile is discussed from the aspects of its structural form and functional features.The kind of field medicine automobile which is made to supply medicine timely and efficiently to meet the demand of field battle,can improve the effectiveness of medical supply and management with standardization,informationization,ordering and facilitation.

Objective To study the effect of small interfering RNA(siRNA) of human papillomavirus(HPV) 18(E6) gene on apoptosis of HPV-related cervical HeLa cell line.Methods siRNA targeting HPV18 E6 mRNA was designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA sequence were then transfected into HeLa cells via Lipofectamine()~(TM)2000.Cell viability was determined by MTT assay.Apoptosis was detected by morphological observation and flow cytometry analysis.The expression level of HPV18 E6 mRNA was assayed by RT-PCR.Results The cell growth and viability of(siRNA) transfected group were significantly inhibited(P

Objective To investigate the hysteromyoma treated with the gonadotropin releasing hormone agonist(GnRH-a)and the impact on immunologic function.Methods 130 cases selected from the patients with hysteromyoma enrolled from January 2016 to December 2016 in hospital have been randomly divided into two groups.The control group has been treated with mifepristone while the study group GnRH-a.Results The sizes of uterus and myoma of both groups have no significant difference before treatment.After treatment, both of them are significantly improved, and those of the study group is significantly smaller than that of the control group(P<0.05).The levels of serum sex hormone and immunologic function of both groups have no significant difference before treatment.They are both improved after treatment, and the study group is significantly better than the control group(P<0.05).Conclusion Applying GnRH-a therapy to the patients with uterine myoma benefits for improving the therapeutic effect, which can improve the serum sex hormone levels and immunologic function.

Objective To analyse effect of nifedipine sustained tablet on hemorheology in the treatment of patients with hypertension complicated with coronary disease.Methods 70 cases with hypertension complicated with coronary disease February 2014 to February 2015, were randomly divided into observation group and control group.35 cases in each group.Observation group received nifedipine treatment, control group were treated with conventional therapy with aspirin, recorded and analyzed related indexes and clinical efficacy of two groups patients after treatment.Results After treatment, patients plasma viscosity and hematocrit values were (1.59 ±0.12)mPa? s, (0.42 ±0.02)%, higher than control group (1.69 ±0.13) mPa ? s, (0.46 ±0.03)%(P<0.05); after treatment whole blood viscosity in observation group (high shear) and whole blood viscosity (low shear) values were (4.95 ±0.41),(9.87 ±1.01) mPa? s, lower than control group (5.89 ±0.43),(10.76 ±0.89) mPa? s(P<0.05); After observation group total effective rate was 91.43%, higher than 71.43%( P<0.05 ) .Conclusion Effect of nifedipine sustained-release tablets in patients with hypertension complicated with coronary disease is sure, precise, and could be compared to conventional treatment alone.It has no significant adverse reactions, can effectively improve hemorheology in patients with hypertension, hypertension complicated with coronary disease.

Objective:To explore the influence of diabetic self-management education on blood glucose control and inflammatory factor levels in inpatients with diabetes mellitus (DM).Methods:A total of 168 DM patients,who hospitalized in department of endocrinology in 163rd hospital of Chinese PLA from Jun 2012 to Jun 2013,were ran-domly divided into self-management education group (self-management group,n=88)and routine health education group (routine management group,n = 80).Self-management group received self-management education course namely AADE7TM ,provided by full-time DM health education staff,including healthy eating,being active,monito-ring,taking medication,problem solving,healthy coping and reducing risks,while routine management group re-ceived routine DM health education.Blood glucose control,changes of levels of myeloperoxidase (MPO),tumor necrosis factor-α(TNF-α)and intercellular adhesion molecule-1 (ICAM-1)were examined before and after interven-tion.Results:Compared with before intervention,there were significant reductions in levels of fasting blood glucose (FBG),postprandial blood glucose (PBG)and glycosylated hemoglobin (HbA1c)in both groups after interventions (P <0.05 all).Compared with routine management group,there were significant reductions in levels of FBG [(7.1 ±1.3)mmol/L vs.(6.0±1.1)mmol/L],PBG [(9.5±1.6)mmol/L vs.(8.8±1.4)mmol/L]and HbA1c [(7.9 ±1.5)% vs.(7.2±1.1)%]in self-management group,P <0.05 all.Before discharge,there were significant de-crease in inflammatory factor levels in both groups compared with before intervention (P < 0.05 all).Compared with routine management group after intervention,there were significant decrease in inflammatory factor levels [TNF-α:(201.0±57.3)pg/ml vs.(100.7±49.2)pg/ml,ICAM-1:(25.2±3.6)ng/ml vs.(18.2±2.9)ng/ml] in self-management group,P < 0.05 both.Conclusion:Self-management education provided by full-time diabetic health education staff is superior to routine diabetic health education method in controlling blood glucose and decrea-sing inflammatory factor levels,indicating it can improveβcell function and relieve insulin resistance.

Objective　Exploring how to check the proportional hazards assumption of the Cox model,and the solutions to non-proportional hazards between the covariates and the hazard function.Methods　With a example data set of Ⅲc stage ovarian serous cystadenocarcina,illustrating how to use graphical methods to check the proportional hazards assumption of the Cox model.Results　the predictor of post-surgery administering medicine times violated the proportional hazards assumption of the Cox model.Conclusion　when using the Cox model to analyze the predictors of survival time,checking whether the predictors violate the proportional hazards assumption of the Cox model or not should be paid attention to.

Objective To study the clinical effect of surgery combined with antifungal drug in treatment of fungal nose-sinusitis.Methods The clinical data of 108 cases with fungal nose-sinusitis were retrospectively analyzed.All patients were treated with nasal endoscopic sinus surgery.According to whether the patients received antifungal drugs after low-temperature plasma-assisted surgery,the patients were divided into the treatment group and the control group.The treatment group(56 cases) was given antifungal fluconazole after low-temperature plasma-assisted endoscopic sinus surgery.52 cases in the control group were washed by 0.9％ sodium chloride solution after conventional endoscopic sinus surgery.The patients were followed up for 6 months to 2 years,the therapeutic effect and recurrence were compared between the two groups.Results 56 patients of the treatment group,49 cases were cured,7 cases improved,no recurrence.52 cases in the control group,47 cases were cured,improved in 4 cases,ineffective in 1 case,3 cases of recurrence.The recurrence rate of the treatment group was significantly lower than the control group (x2 =4.82,P ＜ 0.05).Conclusion The low-temperature plasma-assisted surgery combined with antifungal drug.in the treatment of fungal nose-sinusitis has exact efficacy,and has significant effect on the prevention and reduce the relapse rate,which worthy of clinical promotion.

To introduce composition,the testing principle of the urine analyzer,To reduce the failure rate, extend the service life of the instrument and improve the accuracy of diagnosis. Make the maintenance system ,according to the system of equipment maintenance maintain the urine analyzer daily and monthly. The maintenance plays an important role in reducing the failure rate, extending equipment life and improving the accuracy of diagnosis.