Objective To establish a method for determining Ganciclovir concentration in dog serum by RP-HPLC.Methods The serum was determined by high performance liquid chromatography.A C_(18) column(5 ?m,250?4.6 mm) was applied.The mobile phase was 15 mmol/L potassium phosphate buffer(pH 2.5) containing 0.25% acetonitrile.The flow rate was 1.75 ml/min.The detector set at 254 nm.Column tempetature was 40 ℃.Results The linearity was excellent over the range of 0.050 25-100.5 ?g/ml;The methodological recovery ranged from 96.565% to 105.718%.The within-day and between-days relative standard deviations(RSD) were less than 1.400% and 3.800% respectively.Conclusion The elaborated method is sensitive,precise and can be used in the pharmacokinetic studies.

OBJECTIVE:To prepare ganciclovir dispersible tablet and to observe its dissolution property in vitro.METHODS:The dosage of microcrystalline cellulose and crospolyvinylpyrrolidone in the formula were optimized by orthogonal test;The content of ganciclovir was determined by UV spectrophotometry method;Dissolution rates were compared among ganciclovir dispersible tablet,Ganciclovir common tablet and Ganciclovir capsule.RESULTS:The optimized formula dosage of microcrystalline cellulose and crospolyvinylpyrrolidone were40%and10%respectively;The linear concentration range for ganciclovir was2～20?g/ml(r=0.9999,n=6);The average recovery was(100.21?0.38)%;The mean values of intraday and the inter-day precision were0.22%and0.32%respectively;The ganciclovir dispersible tablet could completely disinte-grated within30s,T 50 was(0.03?0.001)min and T d was(0.18?0.006)min,which were all lower than those of the common tablet and capsule(P

Objective:To investigate the participation of clinical pharmacists in the drug treatment regimen and the methods of pharmaceutical care. Methods:Clinical pharmacists participated in the treatment of one case of cervical malignant melanoma and pro-vided the comprehensive pharmaceutical care, including making the individualized anti-tumor treatment plan, calculating the dose, mo-nitoring the adverse drug reactions. Results: The therapeutic schedule suggested by clinical pharmacists was adopted to improve the clinical outcomes and reduce the adverse reactions. Conclusion: The participation of clinical pharmacists in the practice of clinical drug therapy can improve the level of drug therapy.

Objective To investigate the'expression of Survivin in breast cancer tissue microarray, and its implication to clinic pathology and prognosis. Methods The expression of Survivin was studied in 80 cases of breast disease tissues ( 18 cases of hyperplasia of mammary glands and 62 cases of breast canc-er) and 20 cases of adjacent normal tissues by S-P immunohistochemisty to analysis the relationships be-tween its expression and history grade, clinic stage and lymphaden metastasis. Results The positive rates of Survivin were 0(0/20) in normal mammary glands, 33. 3% (6/18) in hyperplasia of mammary glands and 72. 5% (45/62) in breast cancer. There were significant difference between them (X~2= 34. 62, P <0. 01 ). The expression of Survivin was closely correlated to the stage and lymphaden metatasis and survival time ( P <0.05 or P <0.01), and it was negatively correlated to the histological grade ( P >0.05).Conclusions The expression of Survivin shows an ascending tendency in the carcinogenesis and progres-sion of breast cancer. The Survivin can promote the invasion and the metastasis of the cancer cell, and it is closely correlated to the prognosis.

Hepatic fibrosis is a wound-healing response to liver injury, which has complex celluar and molecular mechanisms. Animal models that similar to human hepatic fibrosis are important to explore further pathological mechanisms and more effective drug therapy for hepatic fibrosis. This article reviews recent progress in animal models and antifibrotic therapy related to hepatic fibrosis.

Indices of angiogenesis are increased in synovia from patients with rheumatoid arthritis, and vascu-lar proliferation may contribute to the pathogenesis of synovitis, pan nus growth, bone and cartilage destruction, and osteophyte formation. Many proan giogenic factors are expressed in the synovium in RA. They have been shown to ha ve important effects in the angiogenic process in RA. So the blockade of angioge nesis appears to be a promising avenue for the future treatment of RA.

Objective To analyze the clinical curative effect and the impact for denture health by using a fixed denture to repair denti-tion defect of the elderly.Methods A total of 90 elderly patients with dentition defect(118 with tooth)were selected in our hospital from January 2012 to September 2013,who were divided into control group and observation group after the relevant examination,45 cases in each group.The patients in control group were treated by removable dentures,and patients in observation group received fixed denture repair.All patients were followed up for 2 years,the health status and the denture bleeding index between two groups were compared and evaluated by patients feedback and chewing ability tests.Results The clinical total effective rate of observation group after treatment was obviously higher than that of control group,the difference was statistically significant(P <0.05).The health effective rate of abutments of the observation group after 2 years was significantly higher than that of the control group,the difference was statistically significant(P <0.05).Bleeding index of two groups after repair were significantly lower than those before,the difference was statistically significant(P <0.05),which reduced more obviously than control group,the difference was statistically significant(P <0.05).Conclusion The fixed denture has high clinical value for elderly patients with dentition defect,which can effectively repair the complex tooth and tooth defect,with good effect and high safety.

Cholelithiasis has still been a common and endemic disease damaging people's health in our country.Management modes and prognosis of this disease have been changed and improved greatly in the past fifty years.Based on our own clinical practice and experience in the People's Hospital of Hunan Province,we reviewed aspects concerning on diagnosis,principles and technical considerations of surgical treatment,summarized background and changes in the past almost half century,which reflected our unremitting efforts and distinctive contributions in management of cholelithiasis.Our experience demonstrated that cholelithiasis should not be considered as a gradually disappearing disease,difficulties and perplexities in dealing with such disease would still be arduous challenges for surgeons,although rates of stone residue and reoperation decreased dramatically.

Background:Gastric cancer is the result of comprehensive interactions among Helicobacter pylori(Hp)infection, environmental factors and host genetic factors. It has been demonstrated that rs2294008 polymorphism in prostate stem cell antigen(PSCA)gene is associated with increased risk of non-cardia gastric cancer. Aims:To investigate the relationship between PSCA rs2294008 polymorphism and precursors of gastric cancer. Methods:A total of 398 patients with gastric intestinal metaplasia( n = 328)and intraepithelial neoplasia( n = 70)from Nov. 2009 to Nov. 2015 at the Qingdao Municipal Hospital were enrolled,and 416 healthy subjects were served as controls. Genotype of PSCA rs2294008 was determined by direct DNA sequencing of PCR products,and Hp infection was examined by rapid urease test. Results:Significant difference in frequencies of CC,CT and TT genotypes of PSCA rs2294008 was observed between case and control groups(P = 0. 011);the frequency of TT genotype was significantly higher in case group than in control group (16. 3% vs. 9. 4% ,P = 0. 003). Compared with individuals carrying CC genotype,TT genotype carriers were found to be associated with a higher risk of precursors of gastric cancer(oR = 1. 840,95% CI:1. 174 ~ 2. 886). Taken individuals negative for Hp infection and carrying C allele(CC + CT)as reference,risk of precursors of gastric cancer was significantly increased by Hp infection(oR = 2. 389,95% CI:1. 799 ~ 3. 173)and Hp infection in combination with TT genotype (OR = 3. 335,95% CI:1. 935 ~ 5. 749),whereas TT genotype alone only slightly increased the risk(OR = 1. 783,95%CI:0. 900 ~ 3. 530). Conclusions:PSCA rs2294008 polymorphism is significantly associated with susceptibility to precursors of gastric cancer and Hp infection might further increase the risk in TT carriers.