Glaucoma is the second leading cause of irreversible blindness worldwide.The treatment of glaucomatous eyes is a long-term procedure.Laser treatment is becoming one of the three major methods to lower intraocular pressure (IOP) of glaucomatous patients.Selective laser trabeculoplasty (SLT) mainly targets to the pigment cells on the trabecular meshwork and makes it easier for fluid to flow out of the front part of the eye,decreasing pressure in the eye.But,the actual mechanism of this surgery is below understood now.Compared with other laser therapy,SLT uses a lower-level laser to open the drainage angle of the eye,and therefore cause rare or slight complication,so it is thought to be a repeatable therapy to the patients who needs further treatment.The principle,clinical application,efficacy,safety and study progress about SLT for glaucomatous eyes are reviewed.

Drug-induced liver injury (DILI) is a leading cause responsible for the failure of drug development and for the withdrawal of commercial drug products.The high frequency of DILI is due in part to the physiology of the liver,since in many cases elimination of drug molecules from the body is dependent on hepatic clearance via either metabolism or biliary excretion.Many of the mechanistic details underlying DILI remain poorly defined in spite of extensive studies of the pathogenesis.In this regard,metabolomics may become a powerful tool for investigation of DILI,leading to better mechanistic understanding and biomarkers identification.

Objective:To establish the granulocyte-differentiation model of the HL60 cells which are treated with All-trans retinoic acid(ATRA),and to use the modified two-dimensional electrophoresis conditions to analyze the differences of protein expression between treated and untreated HL60 cells.Methods:HL60 cells were induced through treatment with All-trans retinoic acid(ATRA).For selection of the appropriate drug concentration and induction time,MTT and flow cytometry are used to detect the HL60cell proliferation and the expression of differentiation antigens CD11b respectively.Cellular chemical staining was used for the verification of the differentiation of the treated HL60 cells.The protein of HL60 cell lines could be separated by modified two-dimensional electrophoresis(2-DE).PDQuest software was used to analyze the different protein expression between treated and untreated HL60 cells.The protein was analyzed by matrix-assisted laser desorption -time of flight mass spectrometry(MALDI-TOF).Results:ATRA could inhibit HL60 cell proliferation,and with the increase in drug concentration,the effect of inhibiting was more significant.Treated with 2? M ATRA for five days,there were more than 90% of HL60 cells expressing antigenCD11b.Cellular chemical staining also showed that ATRA could induce HL60 cells to granulocyte cells.By the analysis of modified 2-DE and PDQuest software,25 protein spots was detected in untreated cells,while 15 protein spots was promoted Some of them were oncogene protein and suppressor gene protein,while some of others are involved in apoptosis.Conclusion:ATRA could induce HL60 cells to granulocyte cells in selected drug concentration and induction time.Using the modified two-dimensional electrophoresis conditions,different protein expression can be found from the traditional two-dimensional electrophoresis.

Craniocerebral Trauma ; surgery ; Humans ; Maxillofacial Injuries ; surgery ; Reconstructive Surgical Procedures ; methods

The article summarizes the questions of clinical teaching of advanced urologists,and find out some measures to improve the clinical teaching quality of advanced urologists.

Objective To investigate the influence of newborns born to mother with systemic lupus erythematosus (SLE).Method The clinical data of SLE mothers and their infants bern in the obstetric and were admitted to the neonatal ward ward of the First Affiliated Hospital of Guangxi Medical University from July 2012 to March 2015 were studied retrospectively.The infants were divided into active SLEactivity group and stable SLE group.The incidence of preterm birth,small for gestational age (SGA),cardiac conduction block,anemia,and thrombocytopenia were compared between the two groups of SLE mothers.Result A total of 66 infants were included in SLE mothers,including 14 cases (21.2％) of preterm infants and 18 cases of SGA (27.3％).14 cases belonged to the active SLE group while 52 cases belonged to the SLE stable group.When comparing the 2 groups,there were no differences found on the rates of preterm infant and small for gestational age (P ＞ 0.05).The cardiac conduction block,anemia and thrombocytopenia happened separately in three cases of the active group,which had not seen in the SLE stable group.There was no statistically significant difference between the two groups (P ＞ 0.05).Of the 66 cases,2 were diagnosed with neonatal lupus erythematosus (NLE) with an incidence of 3％.Conclusion SLE mothers with an active disease 10 days before delivery did not significantly increase the incidence of preterm infants and SGA,but were at risk of NLE.

Objective To investigate the effect of dexamethasone(DXM)and fructose-1,6-diphosphate(FDP)on cardial troponin I(cTnI)and MB isoenzyme of creatine kinase(CK-MB),and ultrastructure of myocardial cells in rats with endotoxemia.Methods Eighty SD rats were randomly divided into 4 groups:sodium chloride group(NS group,n=8),9 g?L-1 NS 1 mL,ip;lipopolysaccharide group(LPS group,n=24),administered with endotoxin(5 mg?kg-1,ip);DXM group(n=24):received DXM(5 mg?kg-1,ip)after injection of LPS 1 h;FDP(n=24)group,received FDP(1 g?kg-1,ip)after injection LPS 1 h.Then,they were sacrificed at 6 h,12 h,24 h and 72 h after injection.CK-MB and cTnI in blood were detected with chemiluminescent techniques,and myocardial pathological damage was observed under the light and transmission electron microscope.Results Compared with control group,in LPS group,the serum cTnI and CK-MB were increased significantly from 6 h to 24 h with time going by(P

Autoimmune pancreatitis (AIP), which is always associated with autoimmune manifestations, was introduced in 1995 and has been recognized as a type of chronic pancreatitis. Despite numerous studies in Japan, Europe and the United States in recent years, no consensus has been reached about the diagnostic criteria for AIP that may be difficult to distinguish from pancreatic cancer(PC). Nevertheless, the results find it dramatically responds to steroid therapy.

Objective To evaluate the efficacy of oxycodone hydrochloride for prevention of fentanylinduced cough during induction of anesthesia in patients.Methods One hundred patients,aged 18-64 yr,weighing 45-74 kg,of ASA physical status Ⅰ or Ⅱ,scheduled for elective surgery under general anesthesia,were randomly divided into 2 groups with 50 cases in each group:control group (group C) and oxycodone hydrochloride group (group O).In C and O groups,normal saline 5 ml and oxycodone hydrochloride injection 0.1 mg/kg (in 5 ml normal saline) were injected,respectively,5 min later fentanyl 3 μg/kg was injected intravenously over 5 s,and 2 min later other drugs for induction of anesthesia were given.The development and intensity of cough were observed within 2 min after fentanyl injection.Results Compared with group C,the incidence of cough was significantly decreased and the intensity of coughing was mitigated in group O.Conclusion Intravenous oxycodone hydrochloride 0.1 mg/kg can decrease the development and intensity of fentanyl-induced cough during induction of anesthesia in patients.

Objective To determine and evaluate the method of localization of anal verge by mutislice spiral CT. To provide an imaging reference for operative guidance of low-rectal cancer. Methods Forty eight consecutive adult patients suspected of abnormalities other than rectal disease were evaluated with abdominal and pelvic CT scans since August, 2009. They were divided into two groups based on sex and age. There were 23 men and 25 women. The ages of young group were 28 to 50 years and the average age was 41 years. The ages of elderly group were 52 to 81 years and the average age was 64 years. A small cotton ball dipped with contrast media was put at the anal verge as a marker and CT scans were performed with 64-slice spiral CT scanner. The distances between the cotton balls and the lower margin of the pubis combination (La), the lower margin of the 5th sacral vertebra (Lb), the inferior aperture of minor pelvis(Lc) and the lower margin of the basement of external anal sphincter (Ld) were measured on the midsagittal images obtained by MPR. The averages, the standard deviations(s), the 95% and 80% confidence intervals of La, Lb, Lc and Ld were calculated. We took the intervals of ± 1.96 s or ± 1.28 s ＜ 5 mm as the standard of meeting the clinical requirement. The distances meeting the clinical requirement between different sex groups or age groups were compared with t test. Results The Ld was (9.8 ± 1.2) mm, ofwhich intervals of ± 1.96 s and ± 1.28 s were 4. 8 mm and 3.0 mm respectively, meeting the clinical need.This distance was also measured conveniently. There were no significant differences ( P ＞ 0.05 ) between two different sex groups [ male group, ( 10.0 ± 1.2) mm], female group, (9.6 ± 1.2) mm and between two age groups [ young group, (9.6 ± 1.2) mm, elderly group, ( 9.9 ± 1.3 ) mm ]. Conclusions The lower margin of the basement of external anal sphincter was a useful anatomic lanmark for localizing the anal verge,and could be definitely identified on the middle sagittal pelvic CT image. The distance between the structure and anal verge is constant enough and can be used in measuring distance from low rectal lesion to the anal verge.