Objective:To observe the effects of triptolide(TP) on retinal gonlion cells of SD rats with experimental autoimmune encephalomyelitis(EAE),and to analyze its possible mechanism.Methods:EAE models were induced in SD rats,the TP group received triptolidedayly intraperitoneal injection from immunization day to 14 days.The pathological changes in optic nerve were observed,the expression of MCP-1,GFAP and the apoptosis of RGCs in retina were detected.Results:Demyelination and inflammatory cell infiltration showed in optic nerve,MCP-1 appearred in the retina at the initial stage,and begine to decline after onset 7 days.The expression of MCP-1 and GFAP were lower in TP group,compared with the EAE group with statistically significant difference(P0.05).Conclusion:Triptolide inhibit the expression of MCP-1 and GFAP,have no significant effects on the apoptosis of RGCs.

Carrying out targeting an oncolytic adenovirus strategy in gene therapy of pancreatic cancer is a new direction.Currently,related research including vitro test and animal models vivo test,targeted strategies on molecular biology and gene level are carried out.Some of oncolytic adenovirus drugs have entered phase Ⅲ clinical trials.Oncolytic adenoviruses alone or in combination with other treatments,can enhanced anti-tumor effect.

A novel H17N10 subtype of the influenza A viruses was found in bats in 2012. Protein sequence and structural analyses revealed that the HA17 and NA10 proteins of this strain are different from corresponding ones in known influenza A subtype viruses. Both HA17 and NA10 proteins cannot bind to sialic acid,which indicates that they may have novel functions. This article briefly describes the state of current research into the H17N10 subtype of bat influenza A virus.
Animals ; Chiroptera ; virology ; Influenza A virus ; classification ; genetics ; isolation & purification ; metabolism ; Orthomyxoviridae Infections ; veterinary ; virology ; Viral Proteins ; genetics ; metabolism

Objective To evaluate clinical value of tumor biomarkers in ascitic fluid and serum in differentiating benign from malignant ascites and their correlation. Methods From July 2006 to July 2009,67 patients with ascites undergone abdominal paracentesis in Renmin Hospital of Wuhan University were enrolled in the study and divided into malignant and benign groups according to their etiology. Levels of tumor biomarkers in ascitic fluid and serum were determined and their correlation were analyzed. Diagnostic sensitivity and specificity of tumor markers were evaluated. Results There was statistically significant difference in levels of cancer antigen 199 (CA199) in serum and ascitic fluid between the malignant group and the benign one ( P ＜ 0. 01 ), and level of cancer embryonic antigen (CEA) significantly increased in ascitic fluid (P ＜ 0.05 ). Levels of CA199 and CEA in serum were significantly higher than those in ascetic fluid in the benign group (P ＜ 0. 01 and P ＜ 0. 05 ), and level of CA125 was significantly lower in serum than that in ascitic fluid (P ＜ 0. 01 ). Level of alpha-fetal protein (AFP) in serum significantly correlated with that in ascitic fluid in the benign group (r =-0. 992, P ＜0. 01 ). In the malignant group, levels of CA199, CEA and CA125 were significantly higher in ascitic fluid than those in serum (P ＜0.05 or P ＜0. 01 ). Levels of CA199 and CEA in serum significantly correlated with those in ascetic fluid in the malignant group (r =0. 746 and 0. 572, respectively, P ＜0. 01 ), and level of AFP in serum also correlated with that in ascetic fluid (r=0. 384, P ＜0. 05). Ratios of levels of CA199 and CEA in ascetic fluid to those in serum (F/S) were significantly higher in the malignant group than those in the benign group (P ＜0.05 or P ＜0.01). Use of combination of CA199, CEA and CA125 determinations showed a higher sensitivity and specificity in differential diagnosis for benign and malignant ascites (P ＜0.05). Conclusions Determinations of CA199 and CEA are beneficial for differentiating benign ascites from malignant one. Determinations of tumor biomarkers in serum can not fully replace those in ascetic fluid. Combined determinations of CA199, CEA and CA125 can increase their sensitivity and specificity in diagnosis for malignant ascites.

Objective To explore influence factors of gerontal patients with lung squamous carcinoma after surgical therapy . Methods 210 gerontal patients with lung squamous carcinoma were accepted surgical therapy and adjuvant therapy ,and were fol-lowed up .The survival rates of 1 ,3 and 5 years after the surgery were evaluated by Kaplan-Meier ,and influence factors were ana-lyzed by Cox regression .Results The 1 ,3 and 5 years survival rates were 89 .0% ,68 .6% and 56 .2% ,respectively .The survival rates were significantly influenced by the smoking history ,the abnormal degree of mediastinal lymph node ,TNM stage ,the class number of lymph node dissection ,and the class number of mediastinal lymph node dissection(P<0 .05) .The smoking history(χ2 =16 .198 ,P=0 .000) ,the abnormal degree of mediastinal lymph node(χ2 =8 .873 ,P=0 .003) ,TNM stage(χ2 =18 .718 ,P=0 .000) , and the class number of mediastinal lymph node dissection (χ2 =4 .897 ,P=0 .027) were the influence factors of prognostic .Conclu-sion The accurate TNM staging and mediastinal lymph node with image examination could be more appropriate to operative indica-tions ,meanwhile ,if smoking was controlled ,the class number of mediastinal lymph node dissection was more .These could be bene-ficial to the survival .

Primary central nervous system lymphoma (PCNSL) is a rare type of malignant lymphoma which originates in the central nervous system without spreading to other systems. It is more predisposable to immunodeficient individuals. However, an increase in the incidence of PCNSL has been also observed in the healthy population in recent years. PCNSL has short phase of clincal release, and easy to relapse with poor prognosis. Some studies have shown that relative prognostic factors were associated with age, performance status, the radiotherapy and chemotherapy modalities, the response to early adminstration of the glucocorticoid,tissue pathologic type and the molecular biologic marks.

The establishment animal model of Graves’ disease contributes to the study of etiology, pathogenesis and therapeutic modalities. After decades of studies and making improvements, the method of building mice model of Graves’disease has achieved a great development. Although there were many reports of animal model building in Graves’disease, as a mature technology A-subunit of thyrotropin receptor( TSHR)-expressing adenovirus was used to establish Graves’disease mice model, which has been accepted widely because of its high efficacy.

OBJECTIVE To investigate the antimicrobial-resistant profile of Staphylococcus aureus isolated from clinical specimens. METHODS The isolates of S.aureus were obtained from all kinds of clinical specimens by routine methods.The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer.Disk-diffusion was used for detecting meticillin-resistant Staphylococcus aureus(MRSA). RESULTS The isolates were mainly isolated from sputa and urines.62.1% of isolates were referred as MRSA.All isolates of MRSA were resistant to ampicillin/sulbactam,penicillin G and cefazolin.No isolates of MSSA were resistant to above drugs.All isolates were susceptible to nitrofurantoin and vancomycin.The resistant rates of the isolates of MRSA to clindamycin,erythromycin,gentamicin,tetracycline,rifampin,sulfamethoxazole and levofloxacin were 70.7%,86.6%,87.8%,82.9%,42.7%,30.5% and 91.5%,respectively,those of MSSA were 31.3%,41.7%,20.8%,20.8%,10.4%,29.2% and 6.3%. CONCLUSIONS The antimicrobial resistance of MRSA is serious,but the antibiotics have high activity to the isolates of MSSA except penicillin G in vitro.Vancomycin has very high activity to the isolates of MRSA in vitro.

Osteoporosis is a major health concern and treatment of primary osteoporosis with anti-osteoporosis medications is needed to reduce fracture risk and burden. Before initiating anti-osteoporosis medications, secondary causes of osteoporosis should be considered and satisfactorily excluded. However, it can be challenging to differentiate primary osteoporosis from secondary osteoporosis, especially in patients with paucity of symptoms or who have less common clinical presentation. Hence, practical tips like Appropriate Care Guide on Osteoporosis forms the basis of initial secondary osteoporosis workup for primary care physicians. Snapshots of secondary osteoporosis are briefly discussed to facilitate “pattern recognition”.