OBJECTIVE To investigate the antimicrobial-resistant profile of Staphylococcus aureus isolated from clinical specimens. METHODS The isolates of S.aureus were obtained from all kinds of clinical specimens by routine methods.The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer.Disk-diffusion was used for detecting meticillin-resistant Staphylococcus aureus(MRSA). RESULTS The isolates were mainly isolated from sputa and urines.62.1% of isolates were referred as MRSA.All isolates of MRSA were resistant to ampicillin/sulbactam,penicillin G and cefazolin.No isolates of MSSA were resistant to above drugs.All isolates were susceptible to nitrofurantoin and vancomycin.The resistant rates of the isolates of MRSA to clindamycin,erythromycin,gentamicin,tetracycline,rifampin,sulfamethoxazole and levofloxacin were 70.7%,86.6%,87.8%,82.9%,42.7%,30.5% and 91.5%,respectively,those of MSSA were 31.3%,41.7%,20.8%,20.8%,10.4%,29.2% and 6.3%. CONCLUSIONS The antimicrobial resistance of MRSA is serious,but the antibiotics have high activity to the isolates of MSSA except penicillin G in vitro.Vancomycin has very high activity to the isolates of MRSA in vitro.

Objective To investigate the retrieval mechanism of prospective memory,spatiotemporal patterns of event-related potentials (ERP) were used to reveal neural correlates of the prospective interference effect. Methods A task of matching two sequential patterns was taken as the ongoing task and the detection of patterns with two salient levels as prospective memory tasks. Experiments were performed in blocks for single ongoing task block and two prospective memory blocks. Results No significant behavioral effect was found for prospective interference [reaction time(ms):control blocks 750.29±161.33,high salience blocks 770.94±174.89,low salience bocks 764.79±174.12,F(1.52,25.82)=0.48,P =0.57 and correction rate(%):control blocks 85.89±8.07,high salience blocks 88.26±7.00,low salience blocks 87.91±7.28,F(1.50,25.57)=2.46,P =0.12].But significant ERP effect was found at bilateral frontal regions from 160 ms to 240 ms for both two prospective memory blocks. Moreover,greater negativity at the right frontal polar region appeared significantly at 360'440 ms for higher salient patterns. Conclusion Prospective memory is retrieved based on strategic monitoring and high salient stimuli may enhance to keep the prospective goal and improve task switching.

Puerarin is one of the most important effective components of Pueraria lobata which exhibited classic estrogen-like biological activities and had remarkable cardiovascular protections in vivo and in vitro experiments. These protections of puerarin are mainly exhibited on improving the myocardial cells membrane potential and arrhythmia based on effecting the Na+, K+ , and Ca2+ channels,resisting myocardial fibrosis damage, diastolic effect on blood vessels, promoting angiogenesis, resisting calcification and atherosclerosis, improving blood flow, antiplatelet aggregation, reducing lipid and resisting diabetes. The main mechanisms are to improve the membrane potential and reduce cardiovascular damage caused by inflammation, oxidative stress and apoptosis, and the main regulated signal pathways are the PI3K/Akt, the NF-kappa B and the caspases.
Animals ; Cardiovascular Diseases ; genetics ; metabolism ; prevention & control ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Isoflavones ; pharmacology ; Oxidative Stress ; drug effects ; Protective Agents ; pharmacology ; Pueraria ; chemistry ; Signal Transduction ; drug effects

Objective To investigate the impact of citreoviridin(CIT) on mRNA expression of mitochondrial respiratory chain synthesis related transcriptional regulation gene,mitochondrial membrane(MMP) potential and reactive oxygen species (ROS) in cardiomyocytes of rat.Methods Viability of rat primary cardiomyocytes treated with different concentrations of CIT (0,1,2,3,4,5,6,7,8,9,10 μmol/L) for 24 h was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method.Based on the MTT curve,median inhibitory concentration(IC50) was calculated using SPSS 13.0.High-,medium-and low-dose groups of CIT(1.650,1.234,0.715 μmol/L) were defined corresponding to 99％,95％ and 90％ of cardiomyocyte viability,respectively.CIT was not added in as the control group.After 24 hours,the mRNA expression levels of peroxisome-proliferator-activated receptor γcoactivator(PGC-1α),nuclear respiratory factor 1 (Nrf1) and nuclear respiratory factor 2(Nrf2) in cardiomyocytes were detected by reverse transcriptase polymerase chain reaction(RT-PCR).Changes of MMP and intracellular ROS were determined by a fluorescence microplate reader using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and 2,7-dichlorofluorescein diacetate (DCFH2-DA) as fluorescent probes.Results Compared with 0 μmol/L CIT group [(89.4 ± 17.6)％],viabilities of rat primary cardiomyocytes treated with 2-10 μmol/L CIT groups[(80.2 ± 20.2)％,(74.4 ± 18.7)％,(63.2 ± 8.9)％,(51.5 ± 18.8)％,(39.0 ± 15.7)％,(22.6 ± 10.5)％,(19.9 ± 4.9)％,(20.7 ± 4.8)％,(18.5 ± 3.3)％] decreased significantly(all P ＜ 0.05).The IC50 value of cardiomyocytes after24 h treatment with CIT was 4.6 μmol/L The PGC-1α mRNA expressions ofhigh-,medium-and low-dose groups(0.431 ± 0.041,0.619 ± 0.031,0.653 ± 0.037) were significantly lower compared to that of the control group(0.776 ± 0.081,all P ＜ 0.05).The Nrf1 mRNA expression of high-dose group(0.358 ± 0.05) was significantly lower compared to that of the control group(0.580 ± 0.098,P ＜ 0.05).Nrf2 mRNA expressions of the high-and medium-dose groups(0.352 ± 0.041,0.472 ± 0.011) were significantly lower than that of the control group (0.667 ± 0.091,all P＜ 0.05).Compared with the control groups[(100.00 ± 0.00)％,(100.00 ± 0.00)％],the MMP levels of high-,medium-and low-dose groups[(55.3 ± 3.3)％,(69.8 ± 4.7)％,(81.8 ± 2.7)％] were significantly lower and the ROS levels[(606.0 ± 46.3)％,(275.0 ± 53.5)％,(158.9 ±29.5)％] were significantly higher(all P ＜ 0.05).Conclusions CIT inhibits the biosynthesis of mitochondria in primary cardiomyocytes and induces oxidative stress.Myocardial injury is caused by cardiomyocyte apoptosis through mitochondrial pathway,which leads to myocardial injury.

Abortion, Induced ; adverse effects ; Acupuncture Therapy ; Adult ; Female ; Humans ; Infertility, Female ; etiology ; therapy ; Pregnancy

Objective: To detect and analyze the mycoplasma infection in human laryngeal carcinoma and to explore the relationship between the infection with the development, progression and prognosis of laryngeal cancer. Methods: Immunohistochemical methods were employed to detect the mycoplasma infection in 214 specimens of different laryngeal lesions and cervical lymphatic tumors metastasized from laryngeal cancer, including 121 laryngeal cancer specimens, 21 laryngeal precancerous specimens, 17 vocal cord polypus specimens. 14 normal laryngeal tissues adjacent to cancer tissues, 9 normal laryngeal tissues opposite to the cancer, and 32 cervical lymphatic tumor specimens metastasized from laryngeal cancer. The infection results were subjected to statistical analysis. Results: The positive rates of mycoplasma 2G10 antibody in laryngeal carcinoma specimens, cervical lymphatic tumor specimens metastasized from laryngeal cancer, laryngeal precancerous specimens, vocal cord polypus specimens, normal laryngeal tissues adjacent to cancer tissues, and normal laryngeal tissues opposite to the cancer were 44.63% (54/ 121),34.38%(11/32),23.81%(5/21),17.65%(3/17),7.14%(1/14),and 0%(0/9), respectively, with those of laryngeal carcinoma specimens and cervical lymphatic tumor specimens metastasized from laryngeal cancer significantly higher than those of other specimens(P<0.05). The positive rate of early stage laryngeal carcinoma (I-II stage, 16.36%[9 /55]) was significantly lower than that of advanced stage laryngeal carcinoma(III-IV, 68.18%[45/66]), (P<0.01). The positive rate of laryngeal carcinoma with cervical metastasis was higher (62.50%[20/32]) than that without cervical metastasis(35.96%[32/89),(P<0.01). The 3- and 5-year survival rates of III-IV stage patients positive of 2G10 antigen (60.0%[27/45], 58.14%[25/43]) were lower than those of patients negative of 2G10 antigen (85.71%[18/21],84.21%[16/19]),(P<0.05). Conclusion: Laryngeal carcinoma has a high infection rate of mycoplasma, which may be related with the development, progression and prognosis of laryngeal carcinoma.

Electroencephalography ; Epilepsies, Myoclonic ; diagnosis ; therapy ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Prognosis ; Spasms, Infantile ; diagnosis ; Time Factors

American Medical Association ; Humans ; Lymphoma, Mantle-Cell ; therapy ; United States

Anodontia ; Humans ; Incontinentia Pigmenti ; therapy

Objective To investigate the effect of arsenic(As2O3) on spermatogenic cell apoptosis and the expression of telomerase activity in adult rats.Methods 40 healthy male Sprague-Dawle rats were randomly divided into four groups and they were treated with As2O3 at doses of 0(control group),0.375,0.75 and 1.5 mg/kg body weight respectively through gavage for 16 consecutive weeks.The numbers of testicular sperm head were counted and the coefficient of testicular viscera,daily sperm production(DSP) were calculated in every group.The apoptosis of germ cell was assessed by in situ terminal deoxynucleotityl transferase mediated dTUP nick end labeling(TUNEL) technique.The activity of telomerase in the spermatogenic cells was determined by immunohistochemistry.Results In the testes of As2O3-treated rats,the coefficient of testicular viscera,the number of testicular sperm head and DSP in moderate and high dose groups decreased significantly than that of control(P