Background and purpose:The prognosis of ovarian cancer is poor and the diagnosis is relatively late. It is needed to search for early diagnosis and treatment for ovarian cancer. This study investigated the expression of serum miRNAs in patients with malignancy or benign ovarian tumor preoperativey and analyzed its correlation with clinicopathological progress and prognostic features of epithelial ovarian cancer.Methods:Forty-eight miRNAs which have been reported to be related to ovarian cancer were ordered. The differential expression of 48 miRNAs in the serum of patients with malignant or benign epithelial ovarian tumors was detected by TaqMan low density array. The differentially expressed miRNAs were further confirmed by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). The relationship between the expression level of selected miRNA and clinical clinicopathological factors, progress and prognosis were analyzed.Results:TaqMan low density array and further RTFQ-PCR showed that only miR-125b was sig-nificantly increased in 135 ovarian cancer patients as compared with 38 individuals with benign tumor. The expression of miR-125b was higher in early stage patients than that in advanced stage patients (P=0.039). The patients without residual tumor expressed more miR-125b than patients with residual tumor (P=0.013). The high level of miR-125b was signifi-cantly correlated with longer progress free survival (PFS) (P=0.003), but not correlated with overall survival (OS) (P=0.069). Conclusion:MiR-125b may play an important role in the pathogenesis of epithelial ovarian cancer and prognosis. It may be a potential gene to predict the recurrence of epithelial ovarian cancer, but the change of gene expression at different stages and its underlying mechanism need further research.

Objective To investigate the effects of N-acetylcysteine (NAC) on the expression of cardiac adiponectin and its receptors in streptozotocin-induced diabetic rats. Methods Thirty-two male Wistar rats were randomly divided into four groups, with 8 in each: untreated control group (C group), NAC-treatment group (CT group), diabetic untreated group (D group) and diabetic NAC-treatment group (DT group). After 8 weeks, plasma glucose and insulin, and cardiomyocyte cross sectional area were measured. Cardiac protein expression of adiponectin receptor 1 and 2 (adipoR1 and adipoR2), AMP-activated protein kinase ? (AMPK?), phosphorylation of AMP-activated protein kinase ?-subunits and glucose transporter 4 (GluT4) were determined by Western blotting. Plasma and myocardial adiponectin levels were measured by radioimmunoassay. Plasma and myocardial free 15-F2t-isoprostane levels were assayed by enzyme immunoassay. Results Compared with C group, the ratio of ventricular weight to body weight and cardiomyocyte cross-sectional area, plasma and levels of free 15-F2t-isoprostane in myocardium and the protein expression of myocardial adipoR1 increased significantly in diabetic rats (D group) (P

Objective To assess the diagnostic value of ChromID for Clostridium Clostridium infection (CDI) on the premise of large sample size,with Meta-analysis.Methods The literature inclusion and exclusion standards were made,and the databases were searched,including PubMed,Cochrane Library,China Biology Medicine disc (CBM),China National Knowledge Infrastructure (CNKI) and so on,using the keywords (ChromID,Clostridium difficile,and chromomgenic agar) along with each of the following words (detection,diagnosis,and infection).Quality assessment of diagnostic accuracy studies (QUADAS) was used to evaluate the quality of the literature.The Meta-disc 1.4 software was used to analyze the data.Results Five studies on 1 851 patients were included in this meta-analysis.The pooled sensitivity was 0.793 (95 ％ CI:0.750 ～ 0.832) for the diagnosis of CDI on 48 h,the pooled specificity was 0.942 (95 ％ CI:0.972 ～0.955),the pooled positive likelihood ratio was 16.817 (95％ CI:7.042 ～ 40.157),with negative hkelihood ratio was 0.124 (95％ CI:0.040 ～0.386) and diagnostic odds ratio for 244.64 (95％ CI:17.711 ～3 379.3).The area under the working curve (AUCSROC) of the subjects was 0.981 4 and the Q* index was 0.939 9.Conclusions Meta-analysis on ChromID for diagnosis of clostridium difficile infection revealedChromID had higher sensitivity,specificity,positive likelihood ratio,and negative likelihood ratio,which can be recommended for clinical use.

Basing on the market multi-target antitumor agent sorafenib, a series of sixteen 4-[4-(2-methyl-aminoacyl-pyridyl)]oxylphenyl aryl thiourea derivatives were designed and synthesized. Their structures were identified by the spectra of 1H NMR, MS and elemental analysis. The evaluation of antitumor bioactivities in vitro was done by MTT method. It was shown that the synthesized compounds had antitumor activities and compounds 1a, 1d, 1i and 1j showed better or equal antitumor activity on sorafenib.

Objective To investigate the chemical constituents of Dimocarpus longan seeds in Sapindaceae.Methods The chemical constituents were isolated from the ethanol extract of D.longan seeds by silica gel column chromatography.Their structures were identified on the basis of physical and chemical properties and spectral analysis.Results One compound was isolated and identified as 2-methyl-1,10-undecanediol,named longandiol(1).Conclusion Compound 1 is a new compound.

OBJECTIVE:To prepare borneol-puerarin liposomes,and to investigate its brain-targeting. METHODS:Film dis-persion ultrasonic method was used to prepare borneol-puerarin liposomes. The morphology of liposomes was observed by TEM;the particle size and Zeta potential were measured by laser particle size analyzer;the entrapment efficiency were measured by sepha-dex gel filtration method. Compared with Puerarin injection,brain-targeting of borneol-puerarin liposomes and puerarin liposomes via intravenous injection of mice tail was studied with relative intake rate and peak concentration ratio. RESULTS:Borneol-puerarin liposomes were spherical or quasi-circular;its mean particle size,polydispersity index and Zeta potential were 226 nm,0.263 and-21.3 mV respectively. The entrapment efficiency were(65.32±2.13)%. Compared with Puerarin injection,relative intake rate of puerarin liposome and borneol-puerarin liposome were 1.68 and 2.58,and peak concentration ratio were 1.15 and 1.42. CONCLU-SIONS:Brain-targeted borneol-puerarin liposomes are prepared successfully.

Objective To explore the application status of Nvivo software in qualitative nursing research and compare the difference between overseas and domestic publications. Methods Six databases were searched and finally 159 qualitative nursing articles were analyzed, the information such as year, region, institution, literature source, impact factor, fund support, theme, theory attachment, analytical approach, data collection approach, function utility were used to included. From inception to January 31, 2016. Results It was found that up to 21%(21/100)publications were from Australia, and up to 18.64%(11/59)publications were from Shanghai in the past 15 years. Responsible institutions were mainly college and hospital. 54.24% of these domestic papers had no fund support, and 78 % of these overseas papers had no fund support. Themes of overseas papers were more various than those of domestic. The utility of Nvivo software overseas proved to be more systematic and deeper. Conclusions The quantity and quality of relevant publications increase dramatically in China in recent years, nevertheless, wider and deeper usage of Nvivo function should be explored deeply. Qualitative nursing researchers should take advantage of benefits of Nvivo, build a holistic view and generalize its comprehensive application.

Objective To study the expression of Mage A1、A2、A3 in breast cancer tissues and four breast cancer cell lines Methods The expression of Mage A1、A2、A3 in breast cancer tissues and four breast cancer cell lines, MCF 7、Sk Br 3、MDA MB 435s and TM40D was detected by reverse transcription polymerase chain reaction (RT PCR) Results The positive expression rate of Mage A in breast cancer tissues was 13/33 (39%), of Mage A1 was 4/33 (12%), of Mage A2 was 8/33 (24%), and of Mage A3 was 7/33 (21%), respectively Both Mage A1 and Mage A3 were positive in breast cancer cell line MCF 7 and Sk Br 3 MDA MB 435s expressed Mage A2 and Mage A3 Mage A3 was positive in TM40D Conclusion Mage genes were often expressed in breast cancer, but expression of Mages varies in the breast cancer cell lines Mage genes encoding proteins are eligible for Mage peptide based active immunotherapy

Objective To evaluate the use of laparoscopic operation in gynecological acute disease. Methods 56 cases of gynecological acute disease patients were treated with different laparoscopic operation according to pathogenetic condition. Results All were exelcymosised urinary canal, liquid diet, out-of-bed actived 6h post operation, ambulation 10h later,no infective fever,postoperative hospital stay 3～5d[mean (3.3?1.1d)] and incisal opening healing well. Conclusion Laparoscope is an effective and safe operation in gynecological acute disease.

Objective To study the serum levels of proinsulin(PI),insulin(INS),proamylin(PA) and amylin(AMY) in abnormal glucose metabolism individuals and to explore the relationship between PI and insulin resistance(IR) and between PA and islet beta cell function.Methods Totally 79 subjects who received Oral glucose tolerance test(OGTT) and insulin release test in our department from March to May 2008 were divided into 4 groups according to the results of OGTT,that is,normal group,impaired glucose regulation(IGR) group,T2DM 1[fasting blood-glucose(FBG)15 mmol/L) group.All subjects underwent examination of anthropometry and serum bio-chemical indicators,HOMA-IR and HOMA-B were calculated.Results No significant difference was found in the serum levels of PI or AMY among the 4 groups,but the level of PA,PI/INS and PA/AMY among the 4 groups did have significant differences(P