OBJECTIVE:To Study the antagonism of Huangshao granule on hepatic fibrosis in rats induced by CCl4.METHODS:60 rats were randomly assigned to blank group,model group,Huangshao granule groups(high,middle and low doses),Dahuang zhechong pill group.All the groups except the blank one were treated with CCl4 to induce hepatic fibrosis model.The primary end points were histopathological change of the liver tissues,activities of ALT and AST,content of albumin,levels of hyaluronic acid(HA),type Ⅲ precollagen(PCⅢ),type Ⅳ collagens(Ⅳ C)and laminins(LN).RESULTS:As compared with model group,in Huangshao granule groups(high and middle doses)and Dahuang zhechong pill group,the hepatic cellular structures showed better improvement,the activities of ALT and AST and serum levels of HA,PCⅢ,Ⅳ C and LN were decreased while the contents of albumin were increased.CONCLUSION:Huangshao granule showed satisfactory antagonism on hepatic fibrosis in rats,and further research and development of which remains to be carried out.

Developing and promoting college students' employment guidance work,setting up the right career ideal and professional concept,helping students make a reasonable career choice in the fierce competition have become the most prior work in the university. The essay mainly studies the present situation of college career guidance and the existing problems,discusses how to improve the employment guidance work in combination with the practice work in the new situation.

AlM: To analyze the effect of corneal limbus stem cell transplantation and amniotic membrane transplantation in treatment of recurrent pterygium with symblepharon.
METHODS:Totally 80 cases of patients with recurrent pterygium and symblepharon were randomly divided into pterygium resection combined with limbal stem cell transplantation group and pterygium excision with amniotic membrane transplantation group, 40 cases in each group. All patients were followed up for 6mo after the operation, the recurrence rate of pterygium and symblepharon were observed.
RESULTS:There were 2 cases of recurrence in the group of corneal limbus stem cell transplantation (5%), 8 cases of recurrence in the group of amniotic membrane transplantation (20%), with statistical difference between two groups (P<0. 05).
CONCLUSlON:Two kinds operation could significantly reduce the recurrence rate of recurrent pterygium, but the corneal limbus stem cell transplantation group has the lower recurrence rate.

Objective:To investigate the effects of telmisartan on the expressions of activin A and its receptors in non-infarction area of left ventricle in the heart failure rats after myocardial infarction,and to clarify the mechanism of improvement effect of telmisartan on myocardial collagen deposition.Methods:A total of 27 rats were divided into sham operation group(n=9),model group(n=20) and telmistartan group(n=10).The left anterior descending coronary arteries of the rats in model group and telmisartan groupwere ligated,and the left anterior descending coronary arteries of the rats in sham operation group were not ligated.After 5 weeks,there were 6 surviving rats in each group.The rats in sham operation group were treated with 0.5%CMC(10 mL·kg-1),the rats in model group were treated with 0.5%CMC (10 mL·kg-1),and the rats in telmisartan group were treated with telmisartan (30 mg·kg-1).All the rats were treated with medicines by intragastric administration for 4 weeks.The whole cardiac hypertrophy index and left ventricular hypertrophy index of the rats were measured;the mRNA expression levels of activin A,ActRⅡA,ActRⅡB,ColⅠ,and ColⅢ in the non-infarction areaof the rats were detected by RT-PCR and the ratio of ColⅠ/ ColⅢ was calculated;the expression intensities of activin A protein in the myocardium tissue in non-infarction area of the rats in various groups were observed by immunohistochemical staining.Results:Compared with sham operation group,the whole cardiac hypertrophy index and left ventricular hypertrophy index of the rats in model group were significantly increased (P<0.01);the mRNA expression levels of activin A,ActRⅡA and ActRⅡB,ColⅠ,ColⅢ and the ratio of ColⅠ/ColⅢ were also increased (P<0.01).The immunohistochemical staining results showed that the expression level of activin A protein in noninfarction area of left ventricle of the rats in sham operation group was lower,and the expression level of activin A protein in non-infarction area of left ventricle of the rats in model group was higher.Conclusion:Telmisartan may improve the myocardial collagen deposition by inhibiting the expressions of activin A and its receptors during heart failure.

Objective To investigate the clinical characteristics of 34 systemic onset juvenile idiopathic arthritis (SoJIA) complicated with macrophage activation syndrome (MAS) and analyzed the gene PRF1,UNC13D,STX11,STXBP2 to figure out the genetic pathogenesis mechanism.Methods The clinical characteristics of 34 SoJIA complicated with MAS were analyzed retrospectively and coding sequences of PRF1,UNC13D,STX11 were amplified and tested.The Chi-square test was applied to compare the distribution of alleles and genotypes frequencies between SLE patients and healthy controls.Statistical significance was defined as P value ＜0.05.Results A total number of 34 SoJIA complicated with MAS were included.Boys accounted for 69％(23/34),and the median age was 6 years.85％(29/34) cases had genetic tests and four SNPS loci were detected:PRF1 c.1061 C＞T (rs885822); UNC13D c.659 C＞T (rs3744007); STXBP2 c.1483 T＞cC (rs10001) and STXBP2 c.1616 A＞G (rs6791).Compared with the control group,genotype and allele frequency of PRF1 rs885822 and STXBP2 rs10001 in MAS cases were statistical significantly different (rs885822:allele frequency x2=4.52,P=0.03 ; genotype frequency:x2=5.52,P=0.02.rs10001:allele frequencyx2=21.33,P=0.00; genotype frequency:x2=19.58,P=0.00).There was no statistical significant difference in genotype frequency and allele frequency of UNC13D rs3744007 and STXBP2 rs6791 between the MAS and control group (rs3744007:allele frequencyx2=1.89,P=0.17; genotype frequency:x2=1.59,P=0.45.rs6791:allele frequency x2=l.69,P=0.19; genotype frequency:x2=2.09,P=0.35).Persistent fever,progressive hepatos-plenomegaly,a sharp decline in blood cells counts,pleural effusion,markedly increased serum liver enzymes,hyperlipidemia were the main characteristics.Some children had mucosal bleeding,neurological dysfunction.More than 82％ children had upper respiratory tract infection before the occurrence of MAS.90％ of children were in remission,while three children had multiple organ failure and died.Conclusion MAS is a fatal complication caused by immune disturbance.Early detection and tre-atment is the key to improve the prognosis.The SNP PRF1 rs885822 and STXBP2 rs1001 may be concurrent with the pathogenesis of SoJIA-MAS.The SNP UNC13D rs3744007 and STXBP2 rs6791 may not participate in the pathogenesis of SoJIA-MAS.

Humans ; Immunity ; drug effects ; physiology ; Infant ; Infant Nutritional Physiological Phenomena ; Nutritional Status ; Vitamin A ; immunology

Objective To explore the clinical effect by using of 654-2 to multigravidae.Methods Among 60 multigravidaes deliverying,30 were treated with 654-2 and oxytocin,the other 30 multigravidaes were treated with oxytocin only for control.The time of dilatation of the cervix,the rate of fetal distress and neonatal asphyxia,the rate of operative delivery and postpartum hemorrhage were observed.Results The time of dilatation of the cervix and the rate of operative delivery were shorter than the control.It was significantly different between the two groups(P0.05).Conclusion 654-2 can promote the first stage of the multigravidae.It can reduce the rate of operative delivery.It is safety by using of 654-2 to short the first stage to multigravidae.

It has been considered that neurons were in a state of terminal differentiation after postmitosis. Recently, however, many data have suggested that the mature neurons may reentry into the cell cycle. Nevertheless, the mechanism of neuronal cell cycle activation remains unclear. The mechanism of cell cycle is an important part in coping with DNA damage in neurons, and the reentry into the cell cycle is to repair the DNA damage. When the damage is too severe to be repaired, it may induce apoptosis.

Objective To investigate the clinical value of laparoscopic assisted transvaginal hysterectomy (LAVH) in gynecologic surgery. Methods LAVH were carried out in 68 patients with myoma of uterus, adenomyosis, and cervical carcinoma in situ,ovarian tumor and so on, which were all indications for uterus resection. Results LAVH were performed successfully in 68 cases, and the successful rate was 100% . Conclusion LAVH has notable superiority of mild trauma,minimal injury and quick recovery in gynecologic surgery.

OBJECTIVE:To set up and improve the procedures and methods for the introduction of new drugs into hospital.METHODS:A supervising system on the introduction of new drugs was established and a programmed management on the introduction of new drugs was put into practice.RESULTS & CONCLUSIONS:A series of measures such as establishing expert database for the evaluation of the introduction of new drugs,assuming supervision throughout the course carried out by discipline inspection committee,increasing clinical pharmacy evaluation and monitoring after the introduction of new drug,etc.have achieved good outcome in the management on the introduction of new drugs,which help promote hospital's fair,transparent and scientific introduction of new drugs.