Objective To introduce a rapid spot test method for nitrite in water to meet the urgent need of nitrite monitoring during accidental pollution. Methods According to the drinking water legislation in Europe (threshold: 0.10 mg/L NO2-), a rapid spot test method for nitrite in water was established by using an optimized composition of reagent mixture and a binary positive/negative response, conducted as follows: a tablet of a mixture of solid reagents containing sulfanilamide, N-(1-naphthyl) ethylene diamine dihydrochloride and sodium chloride was put on a spot test plate of Teflon mould, then, a drop of 30%(V/V) HCl and a drop of water sample were added on the reagent material. Results Under these conditions, all the test samples containing ≥0.10 mg/L NO2- can produce a positive response of the characteristic red-purple color, whereas, the samples will produce a negative response when the level of NO2- was below the limit. The results of real water samples determined with this new method were confirmed by using the photometric method based on the use of the same reagents. Conclusion The method is simple, rapid , accurate, and suitable for field monitoring of nitrite in water.

Animals ; Humans ; Reproducibility of Results ; Selenium ; analysis ; Spectrometry, Fluorescence ; Spectrophotometry, Atomic

Objective To study the effect of small interfering RNA(siRNA) of human papillomavirus(HPV) 18(E6) gene on apoptosis of HPV-related cervical HeLa cell line.Methods siRNA targeting HPV18 E6 mRNA was designed and generated by PCR amplification.The PCR products containing U6 promoter and the siRNA sequence were then transfected into HeLa cells via Lipofectamine()~(TM)2000.Cell viability was determined by MTT assay.Apoptosis was detected by morphological observation and flow cytometry analysis.The expression level of HPV18 E6 mRNA was assayed by RT-PCR.Results The cell growth and viability of(siRNA) transfected group were significantly inhibited(P

The design of field medicine automobile is discussed from the aspects of its structural form and functional features.The kind of field medicine automobile which is made to supply medicine timely and efficiently to meet the demand of field battle,can improve the effectiveness of medical supply and management with standardization,informationization,ordering and facilitation.

Objective To investigate the hysteromyoma treated with the gonadotropin releasing hormone agonist(GnRH-a)and the impact on immunologic function.Methods 130 cases selected from the patients with hysteromyoma enrolled from January 2016 to December 2016 in hospital have been randomly divided into two groups.The control group has been treated with mifepristone while the study group GnRH-a.Results The sizes of uterus and myoma of both groups have no significant difference before treatment.After treatment, both of them are significantly improved, and those of the study group is significantly smaller than that of the control group(P<0.05).The levels of serum sex hormone and immunologic function of both groups have no significant difference before treatment.They are both improved after treatment, and the study group is significantly better than the control group(P<0.05).Conclusion Applying GnRH-a therapy to the patients with uterine myoma benefits for improving the therapeutic effect, which can improve the serum sex hormone levels and immunologic function.

The prevalence of Toxoplasma gondii infection in free-range chickens in Jilin province, northeastern China was investigated. A total of 1095 serum samples were collected from nine administrative regions from July to October, 2012, and antibodies to T. gondii were examined by an in-house enzyme-linked immunosorbent assay (ELISA) using Toxoplasma lysate antigen (TLA). The detection results were confirmed by Western blot. The overall seroprevalence of T. gondii in free-range chickens was 17.6% (95% confidence interval [CI], 15.4–19.4%), ranging from 13.3% (95% CI, 6.3–20.4%) in Siping to 23.6% (95% CI, 15.7–31.6%) in Liaoyuan. There was no significant difference in T. gondii infection among different regions in Jilin province (P> 0.05). The widespread presence of T. gondii infection in freerange chickens of Jilin province implies the wide contamination with T. gondii oocysts in the living environment of people, and free-range chickens might be an important source of infection for humans.

Objective To analyse effect of nifedipine sustained tablet on hemorheology in the treatment of patients with hypertension complicated with coronary disease.Methods 70 cases with hypertension complicated with coronary disease February 2014 to February 2015, were randomly divided into observation group and control group.35 cases in each group.Observation group received nifedipine treatment, control group were treated with conventional therapy with aspirin, recorded and analyzed related indexes and clinical efficacy of two groups patients after treatment.Results After treatment, patients plasma viscosity and hematocrit values were (1.59 ±0.12)mPa? s, (0.42 ±0.02)%, higher than control group (1.69 ±0.13) mPa ? s, (0.46 ±0.03)%(P<0.05); after treatment whole blood viscosity in observation group (high shear) and whole blood viscosity (low shear) values were (4.95 ±0.41),(9.87 ±1.01) mPa? s, lower than control group (5.89 ±0.43),(10.76 ±0.89) mPa? s(P<0.05); After observation group total effective rate was 91.43%, higher than 71.43%( P<0.05 ) .Conclusion Effect of nifedipine sustained-release tablets in patients with hypertension complicated with coronary disease is sure, precise, and could be compared to conventional treatment alone.It has no significant adverse reactions, can effectively improve hemorheology in patients with hypertension, hypertension complicated with coronary disease.

Objective To explore the mechanism of Shenkangwan in preventing the development of diabetic nephropathy(DN)through TGF-?1/Smad signaling pathway.Methods The diabetic rat models were established by intraperitoneal injection of streptozotocin(STZ)in male Wistar rats and randomly divided into 3 groups:model control group,Capoten group and Shenkangwan group.Another 8 normal Wistar rats served as normal control.After the rats had been treated for 8 weeks,the mRNA expressions of fibronetin(FN)and TGF-?1 were detected by RT-PCR and the protein expressions of Smad 2,Smad 3 by immunohistochemistry in the renal tissues.Results During the past 8 weeks,the blood glucose of the rats in the model control group was all over 16.7 mmol/L and they exhibited the symptoms of diabetes mellitus,such as excessive thirsty,extreme hunger,frequent urination and unusual weight loss.The mRNA expressions of FN and TGF-?1 in rat renal tissues of model control group were significantly higher than those in the rats of normal control group(P

Objective:The purpose of the study was to investigate the effects of enteral nutrition on stress response and gut barrier function after surgery for esophageal cancer. Methods: Patients who underwent esophagectomy were divided into two groups. Group PN (28 patients) served as the control group. Group EN (26 patients) received enteral nutrition. Results: The serum total protein, albumin, prealbumin and transferrin decreased after operation, but there were no differences between the two groups. The serum levels of IL-6 were significantly higher on the first and third postoperative days after the operation in two groups (P

The structure, pharmaceutical functions and extraction and isolation methods of chlorgenic acid were reviewed to provide references for the research of chlorogenic acids and the production of the medicines chlorogenic acids.