?AlM:To evaluate the effect and safety of 1. 8mm coaxial microincision phacoemulsification - trabeculetomy with ultra-thin intraocular lens ( lOL ) implants for treating glaucoma complicated with cataract, and to compare with the traditional 3. 0mm small phacoemulsification-trabeculetomy with foldable lOL implantion.
?METHODS: ln this prospective study, 36 patients ( 36 eyes) with glaucoma and cataract in lnner Mongolia Autonomous Regian People’s Hospital were collected and randomly divided into 2 groups. For the small incision group: 18 cases ( 18 eyes ) underwent 3. 0mm coaxial incision phacoemulsification-trabeculetomy with foldable lOL implantion; ln th microincision group: 18 cases ( 18 eyes ) underwent 1. 8mm microincision phacoemulsification-trabeculetomy with ultra-thin lOL implantion. The two groups were recorded for 1wk, 1 and 3mo of visual acuity, corneal endothelial cell density, surgically induced astigmatism, intraocular pressure ( lOP ) , filtering bleb and complications. Pearson’s Chi-square test ande t-test were used to determine differences between the two groups.
?RESULTS:At 1wk postoperatively, visual acuity in the microincision group was better than that of small incision group, the difference was statistically significant ( P<0. 05). At 1 and 3mo, the difference in corrected visual acuity between the 2 groups had no significant difference (P>0. 05). At 1wk, 1 and 3mo, there was a significant different between the 2 groups in surgically induced astigmetism (P<0. 05). At 1wk postoperatively, there was a significant difference in corneal endothelial cells density between 2 groups (P<0. 05). But there were no significant difference at 1 and 3mo ( P > 0. 05 ). lOP reduced after surgy ( microincision group: 15. 26 ±3. 12mmHg, small incision group: 14. 57±2. 86mmHg), there was no significant difference between the 2 groups (P>0.05). There was no significant different between the 2 groups in blebs (P>0. 05). Neither iris injury, posterior capsule rupture nor anterior chamber bleeding complications was found in any groups.
?CONCLUSlON: TBy compared with traditional 3. 0mm coaxial small incision phacoemulsification -trabeculectomy with foldable lOL implantion, 1. 8mm microincision phacoemulsification-trabeculectomy withe ultra- thin lOL implantion can effectively reduce the astigmatism operation. This operation is safe, effective, convenient surgy for treating cataract and glaucoma.

Objective To establish a quality control for Tianmatoufengling Capsule. Methods HPLC method was taken for the determination of Gastrodine in Tianmatoufengling Capsule,A Kromasil C18 column was used,with methanol 0.05% Calcium phosphace(2.5∶97.5) as the mobile phase,and its flow rate was 1.0 mL/min. The detection wavelength was 220 nm. TLC method was used for the identification of Tianmatoufengling Capsule. Results Under the used chromatographic condition,Tianmatoufengling had fine linear responses(r =0.999 9) in the concentration range of 6.016～96.256 ?g/mL,the average recovery was 100.02%,RSD was 2.24%. The method used in TLC-qualitative analysis was simple and sensitive with high specificity,four ingredients identified in Tianmatoufengling Capsule could be detected and all showed specific spots. Conclusion The method employed in this study was convenient and reliable with good repetition. It can be used for quality control in production of Tianmatoufengling Capsule.

OBJECTIVE:To prepare phloroglucinol for injection,establish its quality control and determine its stability.METHODS:The type and dosage of the adjuvant in the formula were optimized and the phloroglucinol for injection was prepared,with its content determined by HPLC.Meanwhile its stability was tested.RESULTS:Mannitol 100.0g was determined as the adjuvant in the formula.The linear range of phloroglucinol was 0.13～ 1.04mg.mL-1(r=1.000 0).No obvious changes were observed at 6 month of an accelerated test or 12 month of long-term storage test.CONCLUSIONS:The preparation is reasonable and feasible in techniques,controllable and stable in quality;and the established method is recurrent,specific and the determination results are within defined margin.

Objective To study the clinical significance of a novel marker of platelet clumps count provided by hematology analyzer in differentiating true thrombocytopenia from EDTA-dependent pseudothrombocytopenia (EDTA-PTCP). Methods Samples from 65 cases of thrombocytopenia (including 15 EDTA-PCTP samples and 50 random samples of true thrombocytopenia) and 50 healthy controls were analyzed using hematology analyzers, and samples with low platelet counts were checked by replacing citric acid and using manual microscope observation to identify true thrombocytopenia from EDTA-PTCP. A novel marker of platelet clumps count was used to differentiate the two diseases for samples anficoagulated with EDTA or citric acid. Results In 65 patients with thrombocytopenia, platelet counts were (48±11)×109/L detected by automatic hematology analyzers. Fifty of 65 cases were true thrombocytopenia which showed low platelet counts [(48±10)×109/L by automated analyzer and (46±11)×109/L by manual assay]. No significance was observed between them (t=-1.26, P0.05). Platelet clumps counts were 86±15. No platelet clamps were detected under microscope. The other 15 cases were EDTA-PTCP [platelet counts were (48±12)×109/L and platelet clumps counts (840±184) were increased significantly by automated analyzer and using EDTA anticoagulant] which showed obviously platelet clumps and no less platelet counts under microscope. After replacing citric acid, platelet counts [(141±13)×109/L by automated analyzer and (134±17)×109/L by manual microscope assay] were increased significantly. No significance was observed between them (t=-1.29, P0.05). Platelet clumps counts (75±12) were decreased obviously compared with EDTA anticoagulant method (t=-6.82, P<0.001). No platelet clumps were detected under microscope. Conclusion Platelet clumps counts may be a useful clinical indicator for monitoring of platelet aggregates, especially for EDTA-PTCP caused by platelet clumping.

Objective To investigate the influence of gender on the enmcement of neuromuscular blocking effects of cisatracurium or rocuronium by sevoflurane. Methods Two hundred and forty ASA Ⅰ or Ⅱ patients, aged 20-60 yr, weighing 45-85 kg, scheduled for elective surgery under general anesthesia were assigned into 2 groups ( n = 120 each): cisatracurium group and rocuronium group. Each group were divided into 4 subgroups according to gender and anesthetics ( n = 30 each): female propofol group, male propofol group, female sevoflurane group and male sevoflurane group. Anesthesia was induced with midazolam, propofol and fentanyl. After loss of consciousness, the laryngeal mask was inserted and the patients were mechanically ventilated. Propofol groups received target-controlled infusion (TCI) of propofol (target plasma concentration 2-6 μg/ml), and sevoflurane groups inhalation of sevoflurane. Cisatracurium 0.15 mg/kg or rocuronium 0.6 mg/kg was injected intravenously 5 min after propofol infusion or after the end-tidal concentrion of sevoflurane was maintained at 1.71% (1 MAC) for 5 min. Neuromuscular block was assessed with accelerograph F (TOF-watch SX). Train-of-four (TOF) stimulation of ulnar nerve was used. The onset time, duration of peak effect and times for recovery of T1 to 25% and TOF ratio (TOFR) to 25% were recorded.Results Compared with propofol groups, the time for recovery of TOFR to 25 % for rocuronium, and the duration of peak effect and times for recovery of T1 to 25 % and TOFR to 25% for cisatracurium were significantly prolonged in female sevoflurane group, the onset time of rocuronium was significantly shortened, while the duration of peak effect and times for recovery of T1 to 25% and TOFR to 25% for rocuronium and cisatracurium were significantly prolonged in male sevoflurane group ( P ＜ 0.05 or 0.01 ). The times for recovery of T1 to 25% and TOFR to 25% for rocuronium and onset time of cisatracurium were significantly shorter in female than in male during sevoflurane anesthesia (P ＜ 0. 05 or 0.01 ). Conclusion The enhancement of rocuronium-induced neuromuscular block by sevoflurane is stronger in male than in female,but there is no gender variation in the enhancement of cisatracurium-induced neuromuscular block by sevoflurane.

Objective To investigate the psychology and personality factors which affect soldiers' psychological health, in order to provide theoretical evidences for the changes in psychology among recruits engaged in tunnelling operation. Methods Using psychological health questionnaire (SCL-90) and Eysenck personality questionnaire (EPQ), 1191 recruits of missile army recruits were tested before and after the mission of tunnelling in 2004~2005 (with the exclusion the dropped outs, those who were transferred or did not complete the whole test period). Results After entering the tunnel, scores of human relationship sensitivity, anxiety, fright and positive items of the recruits were all higher than before the mission or that of servicemen′s norms. In a group of 931 healthy recruits, 157 turned sub-healthy, while 35 of a group of 260 sub-healthy turned healthy. Using EPQ to investigate the personality of these 2 groups, the C value of the former group was found to be on the high side, and this result showed that there had a tendency of concealment and pretension before entering the tunnel. The turnover observed in the latter group maybe due to their extroversion personality. The SCL-90 test of the latter group showed that it was also related to a lowering of score of human relationship sensitivity. Conclusion It shounld be emphasized that it is important to conduct self-education and psychological mediation for the recruits to improve relationship sensitivity and to allay anxiety and fear for the reeruits before and after the tunnelling operation, in order to improve their psychological health status.

Objective To explore whether augmentation index is an independent risk factor for coronary heart disease or not,and the relationship between augmentation index and coronary artery disease severity.Methods In the study,245 subjects who were hospitalized between Dec.2003 and Dec.2004,were classified into 4 groups and their clinical and biological characteristics and the summary of the scores in each of the eight segment and aortic pressure waveform in ascending aorta were recorded respectively.Results The larger the augmentation index was,the more the number of coronary stenosis vessels was.Linear regression analysis indicated that augmentation index was significantly correlated with grade of coronary stenosis.When augmentation index was more than 45%,the sensibility and specificity was 91.5%and 92.6%respectively.In the logistic regression model,augmentation index was the independent risk factor for coronary heart disease,and the odds ratio of coronary heart disease was 1.893.The 95%CI was 1.421~2.521.Conclusion Augmentation index is an independent risk factor for coronary heart disease and a predictor of angiographic coronary artery disease severity.

Objective To investigate the efficacy of injectable osteoinductive material with fibrin sealant(FS) as a carrier compounded with bovine bone morphogenetic protein(bBMP) and bovine fibroblast growth factor(bFGF) for radial defect in dogs.Methods A total of 12 dogs were used in this study.The animals were randomly divided into treatment and control groups with 6 in each.We created a 20-mm bone defect at the upper radius of each dog,and then sutured the subcutaneous tissues and skin around the lesion.After the operation,FS(control) and FS+bFGF+bBMP were given to the two groups respectively by percutaneous injection.To compare the efficacy of the injections,we examined the animals by radiography in 4,8,16,and 24 weeks.The dogs were sacrificed in 24 weeks to obtain the specimens of the bone defect for histological examination and bone mineral density(BMD) determination.Results Radiography showed callus formation in 4 weeks and then osteoneogenesis in 24 weeks in the treatment group;whereas,in the control group,no callus was found around the defect in 24 weeks.In the treatment group,the mean BMD of the diseased radius was significantly higher than that of the healthy leg and that in the control group [(456.33?13.74) mg/cm2 vs(433.33?6.77) mg/cm2(t=2.57,P=0.00) and 0 mg/cm2].By histological examination,the new-formed bone in the treatment group was confirmed integral and dense with intact cortex and continuous marrow cavities in 24 weeks,while the bone defect of the controls were repaired with connective tissues without remodeling of the bone.Conclusion It is effective to repair bone defect in dogs by using injectable osteoinductive material with FS as a carrier compounded with bBMP and bFGF.

Objective To study the effect of injectable osteoinductive materials with fibrin sealant as the vehicle on the reconstruction of segmental radius defect in dog, and to provide an experimental foundation for its clinical application in the future. Methods 20mm bone defects were created in the radius 18 dogs, and in the experiment group b [FS+bFGF+bBMP (bovine BMP)] composite, and in experiment group r (FS+bFGF+rhBMP-2) were injected percutaneously into the defects. A control group (FS) with bone defect only was also included. The reconstruction effect on the segmental dog radius defects was evaluated by radiography, histology, bone mineral density (BMD) at 4, 8, 12, 16, 20 and 24 weeks after operation. Results In the experimental groups with bovine BMP as osteoinductive agent, higher repairing capability and osteogenesis rate were observed in experiment group b (FS+bFGF+bBMP) compared with that of control group (P

Objective To investigate the effect of the injectable osteoinductive material with fibrin glue(FG)as carrier compounded with rhBMP-2 and bFGF on the proliferation and differentiation of marrow matrix cells(MMCs)of rabbit,in order to lay an experimental foundation for clinical application in the future.Method MMCs were isolated and cultured from bone marrow of a 3-day-old rabbit.The effects of the material were investigated in experiment group(group A:FG+bFGF+rhBMP-2),with control groups consisting of control group 1(group B:FG),control group 2(group C:FG+bFGF),control group 3(group D:FG+rhBMP-2)and single control group(group E:free of material).The observation objects included proliferation rate,adhesive rates,expression of type I collagen and alkaline phosphatase,cell growth condition in the material and the ultrastructure of rabbit MMCs with the aid of electron microscopy,histochemistry and cell culturing.The concentration of rhBMP-2 and bFGF in all groups was uniform(1?g/ml).Results The proliferation rates and the adhesive rates of rabbit MMCs in group A was significantly higher than that in group B,group D and group E,but was lower than in group C(P