Decellularized tissues and organs have been widely used in tissue engineering and regenerative medicine applications. The efficiency of cell removal from a tissue depends on the origin of the tissue and the methods applied. Each of these treatment affects the biochemical composition, tissue ultrastructure, and mechanical behavior of the remaining scaffold, which in turn, affect the host response to the material. Herein, we made a review about the most commonly used decellularization methods and their effects upon biologic scaffolds.

Objective To study the relationship between vulnerable plaque and nonlacunar infarction, and analyze distribution regularity of carotid atherosclerotic plaque. Methods One hundred and four patients with ischemic stroke were divided into two groups: nonlacunar infarction group (64 cases) and lacunar infarction group (40 cases) based on imaging and clinical manifestation. Location, number, type,degree of carotid atherosclerotic plaque was recorded and compared between the two groups. Results Two hundred and ninety-seven plaques shown by the imaging were distributed in different areas:9 plaques located in the initial part of the carotid artery, 51 plaques located in the carotid artery, 148 plaques located in the bifurcation of the carotid artery, 27 plaques located in the internal carotid artery and 62 plaques located in the intracalvarium part of internal carotid artery. The percentage of severe or occlusion carotid artery stenosis in nonlacunar infarction group was 29.7% (19/64), which was higher than that in lacunar infarction group [10.0% (4/40)] (P = 0.019 ). The percentage of vulnerable plaque in nonlacunar infarction group was 62.5% (40/64), which was higher than that in lacunar infarction group [47.5%( 19/40)](P=0.004). Multivariate analysis showed that the degree of carotid artery stenosis(OR = 1.740,95% CI: 1.002 -3.021 ) and vulnerable plaque (OR = 2.865,95% CI: 1.047 - 7.813 ) was related with nonlacunar infarction.Conclusions The plaque is more frequently encountered at the bifurcation of the carotid artery. The degree of carotid artery stenosis and vulnerable plaque are the independent risk factors of the nonlacunar infarction.CT angiography can clearly display carotid artery stenosis, morphology and component of plaque, so it will be used as the preferred clinical examination.

Free-DNA exists in body fluid of cancer patients, and the concentration is much higher than that of healthy people, and the free DNA is characteristic of tumor issue. This article makes a summary about the study of free DNA in cancer patients,such as methylation,gene mutation, microsatellite instability (MSI) and loss of heterozygosity( LOH ). It has important significance for tumor dignosis,assessment of prognosis and recurrence.

Objective To provide references for improving the quality of TCM decoction pieces through the analysis on sample inspection of TCM decoction pieces of Huguosi TCM Hospital of Beijing University of Chinese Medicine (hereinafter referred to as our hospital). Methods In our hospital from January 2014 to December 2015, TCM decoction pieces with problems in this sample inspection were categorized according to reasons. Results Quality problems found in the sample inspection were categorized into 3 types, i.e. collection and preparation (69.79%), storage (20.93%) and other problems (9.31%). Cutting in collection and preparation (13.95%), worming in storage (9.30%) and breaking/crumbing (6.98%) were the most obvious problems. Conclusion Strengthening the management of procurement acceptance, strict control of storage and storage conditions, and paying attention to the use of other aspects of management can improve the quality of TCM decoction pieces.

Chronic Disease ; Humans ; Nasal Polyps ; diagnosis ; Phenotype ; Precision Medicine ; Rhinitis ; diagnosis ; Sinusitis ; diagnosis

Based on the analysis of the status quo and development trends of disaster medicine system abroad along with a summary of the present conditions and inadequacies of the same system in China, this paper proposes to build such a system in Hainan Province. It studied, with reference to the specifics of Hainan Province, the characteristics of such a system in the province, involving such aspects as hospital setup, human resource development, construction of research centers, professional training,management of disease control centers, improvement of the hospital self-rescue system, reliable supply of drugs and medical devices, maintenance of disaster medicine information system, logistics support,improvement of assessment system, establishment of rescue-oriented culture, and psychology crisis intervention and promotions.

Objective: To explore the effect of immune reaction induced by alginate on parotid acinar cells in vitro. Methods: Rabbits were immunized from the conjugated alginate- BSA (1.0 mg/kg) by 40-days routine immunity method. ELJSA method was used to examine the titration (valence) of anti-alginate serum. Five groups (group A: contrast, group B: BSA, group C; alginate, group D: anti-alginate serum, group E; alginate + anti-alginate serum) were examined by MTT method at four time points( 1, 6,12 and 24 h). The growth and morphology of parotid acinar cells were observed under inverted phase contrast microscope and scanning electron microscope. Results: Antibody-serum was acquired by routine immunity method, and the titration (valence) of anti-alginate serum was 1: 400. MTT results showed that the proliferation of parotid acinar cells had been limited at 24 h( P <0.05), the other three time points showed no difference. Under inverted phase contrast microscope, a few of acinar cells whose membranes were destroyed after 12 h, some cell contents leaked out. The holes in membrane could be seen early at 6h under scanning electron microscope. Most of the acinar cells were broken at 24 h. Conclusion: The antibody-serum to alginate and immunized rabbit was acquired by routine immunity method. The immune reaction induced by alginate can destroy parotid acinar cells in vitro.

Objective To investigate the relationship between estrogen replacement therapy and female patients with Alzheimer's risk. Methods We searched the PubMed, Springer, CBM, CNKI, VIP database for studies on the es-trogen replacement therapy and women with Alzheimer's disease, retrieval time from database to 2012 December. We evaluated the methodological quality of the included studies, used RevMan 5.0 software to analyze the extracted data. Results We found seven case-control studies which included 1392 patients and 2719 controls. The meta analysis re-sults show that the proportion of estrogen replacement therapy in AD group is significantly lower than that of the con-trol group, the difference has statistically significant [OR=0.68, 95%CI(0.50,0.90), P=0.01]. Conclusions estrogen re-placement therapy has effects on female patients with Alzheimer's disease, and is a protective factor.

Objective:To explore long-term therapeutic effect of TCM on acquired immunodeficiency syndrome(AIDS).Methods:CD_4,CD_8, CD_4/CD_8 ratio and body weight before and after treatment in the patients who had been treated decoction or proprietary medicine of TCM for over 10 years were compared.Results:CD_4 and body weight increased significantly after 30 months of treatment(P

Objective To know rural drinking water safety and the affecting factors in Guangxi Zhuang Autonomous Region.Methods The survey was conducted through a combination of census and spot-checks during January to May 2005,taking villages as the survey units,the basic situation of water supply,coverage of tap water,running of water supply facilities,and the distribution and types of water sources,pollution of water sources,water sources and beneficiary population were investigated.The water samples were collected and tested with related standard methods on July and November,2005.Results The total rural population in Guangxi Zhuang Autonomous Region was 41.424 0 million,13.858 1 million people were served with central water supply,accounted for 33.45%.There were 14 260 township and village level central water supply systems.Of these systems,only 1 157 had water purification facilities,accounted for 8.11%.A total of 475 water samples were tested,the eligible was 25.68%.The eligible rates were 23.71% and 27.57% in rainy and dry seasons,respectively.The bacteriological indexes were the main noneligible indexes,as for total coliform,the eligible rate was less than 40%.The rural population consuming unsafe drinking water was 17.768 6 million,accounted for 42.9%.Conclusion The drinking water safety in rural areas in Guangxi Zhuang Autonomous Region is to be solved urgently.Special focus should be paid to the construction and management of rural drinking water safety projects.