Brain Abscess ; etiology ; Female ; Humans ; Leuconostoc ; pathogenicity ; Male ; Sepsis ; complications ; microbiology

Objective: To investigate the expression of molecular marker ABCG2 of stem cells and tumor angiogenesis-related gene products including vascular endothelial growth factor (VEGF), CD34 and very late antigen alfa-2 (VLA-2α) in human glioma-bearing nude mice and observe the patterns of angiogenesis in transplanted tumors by CD34-PAS double staining. Methods: The human glioma cells were subcutaneously transplanted in 30 nude mice. Six normal brain tissues were as controls. The hematoxylin and eosin staining and immunohistochemical examination were performed to determine the positive rate and the hot-spot of gene product expression. The patterns of angiogenesis were observed under light microscope. Results: The positive rates of ABCG2, VEGF, and VLA-2α expression were all 100% in transplanted tumors. The average percentage of positive cells was (3. 93 ± 1. 438)%, (34. 84 ± 6.212)% (30.33 ± 4.428)%, respectively. The value of micro-vascular density (MVD) labeled by CD34 averaged at 29. 78 ± 5.88. All the three microcirculation patterns, endothelium-dependent vessels, mosaic vessels and vasculogenic mimicry, were present in xenografted human glioma tissue samples. Conclusions: The ABCG2-overexpressed cells tended to be located close to blood vessels. VEGF and VLA-2α play an important role in angiogenesis of transplanted tumors in nude mice. There exist three different microcirculation patterns in high malignant transplanted tumors.

Objective To evaluate the feasibility of cell free fetal DNA(cffDNA)-based noninvasive prenatal diagnosis,we developed a precise technique for fetal sex determining region of Y chromosome(SRY)gene detection using size-fractionated cell-free DNA in maternal plasma.Methods Peripheral blood samples were collected form 117 pregnant women.cffDNA was extracted based on a column absorbent method and isolated by agarose gel electrophoresis.A dulex-polymerase chain reaction(PCR)was used to detected SRY gene and glycerol-dehyde-phosphate dehydrogenase(GAPDH)gene.Results Both SRY and GAPDH gene were detected in 86 cffDNA samples from women bearing male fetuses.And only GAPDH gene was detected in 71 cffDNA samples from women bearing female fetuses.These results had a coincidence whit those of villus or amniotic fluid samples.The specificity and sensitivity reached 100%(117/117)and 100%(66/66),respectively.Conclusion By agarose gel electrophoresis,re-extratedand and dulex PCR,size-fractionated cell-free fetal DNA in maternal plasma can be selective enriched and used to noninvasive prenatal diagnosis of sex-linked disorders and single gene disorders.

Angioplasty, Balloon, Coronary ; instrumentation ; Female ; Humans ; Middle Aged ; Prosthesis Failure ; Stents

AIM: To study the dermal toxicity and absorption of Triptolide Compound Cream. METHODS: 2% azone+1% volatile oil of twig from Camellia. oleifera was used as permeation enhancer to prepare Triptolide Compound Cream. Acute dermal toxicity, irritation a sensitization test on mouse were carried out. And mouse skin in vitro was applied for studying the administration. RESULTS: This compound cream had no acute toxicity, irritation or hypersensitive effects. 2% azone+1% volatile oil had more powerful enhancement effects, after 12 h, amont of drug delivery/unit area Q/( μg/cm~2) and absorption percentage Q% of tfiptolide and meloxican in the preparation reached a platea conventration. The results also showed that there was a good linear correlation between the accumulative permeated amount per unit area and the time. CONCLUSION: The study showed that Triptolide Compound Cream has no toxicity on skin, and there is a good dermal absorption.

ICH GCP requires that all information of clinical trial should be recorded, processed, and stored in a way that allows the accurate reporting, interpretation and verification. A trial master file (TMF) contains all paper or electronic records/documentations related to a clinical trial. As a tool of the retrospective analysis, the TMF profile should be able to reproduce the full procedure of the trial completely. As a part of TMF profiles, both the accuracy and completeness of clinical data management documentation are important in data integrity. It is helpful to learn the workflow of clinical data management in different stage of a clinical trial, to understand which documents are essential, and why the documentation of clinical data management is important for data integrity. This paper elaborates how to perform the good documentation practice of clinical data management, and suggests that both the precise and efficient document management and regular quality control may ensure the high quality of clinical data documentation management on the basis of an intensive awareness of the overall process of clinical data management.
Clinical Trials as Topic ; Data Curation ; standards ; Information Storage and Retrieval ; methods ; standards

Reactivation of hepatitis B virus is a common complication of patients with malignant tumor chemotherapy, and gets more attention in clinicians. But the mechanism of reactivation of hepatitis B virus is not completely understood. There is not a unified definition and the prevention and cure of reactivation of HBV at home and abroad. In this paper, the relevant content of HBV reactivation in recent years is reviewed.

Objective To investigate the treatment effect of recombinant bovine basic fibroblast growth factor (rb-bFGF) in patients with gingivitis and its influence on gingival sulcus bleeding index. Methods Sixty-eight patients with gingivitis treated in our hospital were randomly divided into control group (34 cases) and observation group (34 cases). The control group were given basic periodontal therapy, while the observation group were treated with rb-bFGF. The clinical effects, gingival sulcus bleeding index (SBI) before and after treatment, and the recurrence were compared between the two groups. Results After treatment, the total effective rate (97.06%) of the observation group was significantly higher than that of the control group (70.59%), the difference was statistically significant (P<0.05). The SBI of the observation group was significantly lower than that of the control group at 1 and 2 weeks after treatment (P<0.05). The recurrence rate of the observation group (5.88%) was significantly lower than that of the control group (23.53%) (P<0.05). The incidence of complications such as pain, periodontal abscess, bromopnea and loosening was significantly lower in the observation group (8.82%) than in the control group (32.35%). The difference was statistically significant (P<0.05). Conclusion After rb-bFGF treatment, the symptoms of toothache and gingival swelling disappear, normal function of teeth is restored, gingival sulcus bleeding improves significantly, with fewer adverse reactions and obvious clinical effect.

Objective To investigate the status of Kachin-Beck diseage(KBD)in Xi'an and provide a scientific basis for decision-making in controlling the disease.Methods According to original disease data of KBD,children aged 7 to 12 were chosen to receive clinical and X-ray examination according to the KBD monitoring standard stipulated by the government in 6 villages of 3 countries in Xi'an.The local adults were elinically diagnosed and divided into different groups according to the clinical degrees and their ages.Resuhs Among 373 schod children who had clinical examinations,15 early cases were detected,and the detectable rate was 4.02%(15/373);Among 325 school childrenundergoning by X-ray,1 case was detected,and the detectable rate was 0.31%(1/325).In 5630 adults clinically tested,1402 patients were detected,the detectable rate was 24.90%(1402/5630).The patients distributed mainly in the population aged above 36 years old.Conclusions The situation of KBD control is still serious in Xi'an city.KBD monitoring and health education should be carried on.Furdher Tesearch on treatment methods and drug therapy should be performed.

The adenocarcinoma of the esophagogastric junction (EGJA) is located in a unique anatomical position and at the junction of the squamous epithelium and the columnar epithelium. The biological characteristics of this disease are different from those of esophageal or gastric cancer. The diagnostic classification of EGJA has been subject to controversies, and no gold standard therapeu-tic regimens have been established, especially in the choice of treatment of locally advanced EGJA. Results from large-scale clinical tri-als and imaging technology development showed that the treatment of EGJA has been individualized. Furthermore, this problem high-lights the importance of multidisciplinary collaboration. This article focuses on current progress in studies on EGJA.