Brain Abscess ; etiology ; Female ; Humans ; Leuconostoc ; pathogenicity ; Male ; Sepsis ; complications ; microbiology

Objective: To investigate the expression of molecular marker ABCG2 of stem cells and tumor angiogenesis-related gene products including vascular endothelial growth factor (VEGF), CD34 and very late antigen alfa-2 (VLA-2α) in human glioma-bearing nude mice and observe the patterns of angiogenesis in transplanted tumors by CD34-PAS double staining. Methods: The human glioma cells were subcutaneously transplanted in 30 nude mice. Six normal brain tissues were as controls. The hematoxylin and eosin staining and immunohistochemical examination were performed to determine the positive rate and the hot-spot of gene product expression. The patterns of angiogenesis were observed under light microscope. Results: The positive rates of ABCG2, VEGF, and VLA-2α expression were all 100% in transplanted tumors. The average percentage of positive cells was (3. 93 ± 1. 438)%, (34. 84 ± 6.212)% (30.33 ± 4.428)%, respectively. The value of micro-vascular density (MVD) labeled by CD34 averaged at 29. 78 ± 5.88. All the three microcirculation patterns, endothelium-dependent vessels, mosaic vessels and vasculogenic mimicry, were present in xenografted human glioma tissue samples. Conclusions: The ABCG2-overexpressed cells tended to be located close to blood vessels. VEGF and VLA-2α play an important role in angiogenesis of transplanted tumors in nude mice. There exist three different microcirculation patterns in high malignant transplanted tumors.

Objective To evaluate the feasibility of cell free fetal DNA(cffDNA)-based noninvasive prenatal diagnosis,we developed a precise technique for fetal sex determining region of Y chromosome(SRY)gene detection using size-fractionated cell-free DNA in maternal plasma.Methods Peripheral blood samples were collected form 117 pregnant women.cffDNA was extracted based on a column absorbent method and isolated by agarose gel electrophoresis.A dulex-polymerase chain reaction(PCR)was used to detected SRY gene and glycerol-dehyde-phosphate dehydrogenase(GAPDH)gene.Results Both SRY and GAPDH gene were detected in 86 cffDNA samples from women bearing male fetuses.And only GAPDH gene was detected in 71 cffDNA samples from women bearing female fetuses.These results had a coincidence whit those of villus or amniotic fluid samples.The specificity and sensitivity reached 100%(117/117)and 100%(66/66),respectively.Conclusion By agarose gel electrophoresis,re-extratedand and dulex PCR,size-fractionated cell-free fetal DNA in maternal plasma can be selective enriched and used to noninvasive prenatal diagnosis of sex-linked disorders and single gene disorders.

The adenocarcinoma of the esophagogastric junction (EGJA) is located in a unique anatomical position and at the junction of the squamous epithelium and the columnar epithelium. The biological characteristics of this disease are different from those of esophageal or gastric cancer. The diagnostic classification of EGJA has been subject to controversies, and no gold standard therapeu-tic regimens have been established, especially in the choice of treatment of locally advanced EGJA. Results from large-scale clinical tri-als and imaging technology development showed that the treatment of EGJA has been individualized. Furthermore, this problem high-lights the importance of multidisciplinary collaboration. This article focuses on current progress in studies on EGJA.

Based on special biological characteristics and prognosis, the incidence of gastroesophageal junction adenocarcinoma (GEJA) has rapidly increased over recent years, and its diagnosis and treatment remain controversial. Compared with simple surgery, neo-adjuvant and adjuvant therapies can improve the survival of patients with locally advanced GEJA. Under neo-adjuvant therapy, neo-adjuvant chemoradiation is superior to neo-adjuvant chemotherapy alone for improving the resectability, maintaining loco-regional control, and improving the quality of life of the patient. The combination of this therapy with targeted drugs may further increase the ef-ficacy of GEJA. Most data on GEJA-treated patients were obtained from randomized clinical studies on esophageal cancer or gastric cancer. Thus, prospective randomized controlled studies with a large sample size should be performed to optimize the strategy of neo-adjuvant and adjuvant therapies, and further improve the treatment outcome. In this article, studies on the comprehensive treatment of GEJA were reviewed.

AIM: To study the dermal toxicity and absorption of Triptolide Compound Cream.METHODS: 2% azone+1% volatile oil of twig from Camellia.oleifera was used as permeation enhancer to prepare Triptolide Compound Cream.Acute dermal toxicity,irritation a sensitization test on mouse were carried out.And mouse skin in vitro was applied for studying the administration. RESULTS: This compound cream had no acute toxicity,irritation or hypersensitive effects.2% azone+1% volatile oil had more powerful enhancement effects.after 12 h,amont of drug delivery/unit area Q/(?g/cm2) and absorption percentage Q% of triptolide and meloxican in the preparation reached a platea conventration.The results also showed that there was a good linear correlation between the accumulative permeated amount per unit area and the time. CONCLUSION: The study showed that Triptolide Compound Cream has no toxicity on skin,and there is a good dermal absorption.

Objective To observe the change on living blood cells of peripheral bloodstream in patients with TIA.Methods To observe the behavior of living blood cells in the peripheral bloodstream of 18 patients with TIA using high magnification microscopy system, and compared with 20 healthy individuals.Results Erythrocyte aggregation rate, leukocyte activation rate, thrombocyte activation rate, and the extent of those changes were higher in the TIA group than in the control group. This difference was statistically significant ( P

Objective To study the effects of acute cadmium exposure on the immune function of white blood cells (WBC), plasma interleukin-2 (IL-2) and cortisol levels in rats. Methods Thirty-six male rats were randomly divided into three groups averagely. The control group (group C) and two experimental groups(groupⅠand group Ⅱ) were respectively exposed to 0, 0.5, 1.0 mg / kg body weight (BW) cadmium for 7 days by intraperitoneal injection. Blood samples of the rats were collected on the 4th and 7th day after administration of cadmium respectively and the related parameters were analyzed. Results The BW of rats in groupⅠand group Ⅱ were significantly lower than that of the group C. The WBC counts of two experimental groups were higher than that of the control group. Higher percentage of neutrophiles and lower percentage of lymphocytes were observed in rats of group Ⅰ and Ⅱ on the 7th day after cadmium exposure, while no obvious variations in monocytes(%), eosinophiles(%) and basophiles(%)were observed among the three groups. Blood T-lymphocyte(%) and IL-2 levels in rats of groupⅠandⅡdecreased on the 4th and 7th day after cadmium exposure respectively, while B-lymphocyte(%) increased on the contrary. Plasma cortisol levels in rats of groupⅠ and groupⅡ were higher than that of the group C on the 7th day after cadmium exposure. Conclusion The results showed that acute cadmium exposure could affect the WBC immune function and result in the defect of cellular immune function as well as significant change of adrenal cortex endocrine activities.

To measure carotid intima media thickness(IMT) and to investigate the relationship between carotid atherosclerosis and some major risk factors in elder uremic patients, a cross sectional study was carried out in 30unselected elder uremic patients (16on hemodialysis). Fasting blood sampling for serum lipids, BUN, creatinine, hemoglobin, and echo colour Doppler evaluation of common carotid arteries and heart were performed , BP was measured in all patients (before dialysis day in hemodialysis patients). Relationship between the results and miltifactoral regression analysis were also carried out. 17 patients(56 6%)had carotid IMTwhile 12 dialysis patients(75%) had it. 6patients (20%) had at least one plaque. A significant correlation was found in internal diameter of carotid arteries, IMT and blood vessel resistance between left and right carotid arteries. Carotid IMT had a close relationship with serum BUN, creatinine, cholesterol, systolic BP and heart ejection fraction. In multiple regression models, serum creatinine and cholesterol was significant and an independent predictor of the degree of carotid IMT. In elder uremic patients, carotid IMT is quite common. Elder age, hypertension, degree of renal insufficiency and dyslipidemia are associated with carotid atherosclerosis. Serum creatinine and cholesterol appears to serve as an independent predictor of carotid atherosclerosis, which contributes to the cardiovascular complications and high mortality in elder uremic patients.