Objective To study the angiographie characteristics of coronary heart disease (CHD) patients with type 2 diabetes mellitus.Methods 292 patients with CHD underwent a selective angiography were selected,the characteristics of coronary arteriongraphy were compared in CHD patients with type 2 diabetes mellitus and nondiabetic group.Results In type 2 diabetes mellitus group,the degree of stenosis was higher than that in nondiabetic group (78.01% vs 52.32% )(P < 0.05 ), and the rate of three-vessel disease was higher than that in nondiabetic group (59.57% vs 36.42% )(P <0.05).Conclusion The angiographic characteristics were different in CHD patients with or without type 2 diabetes mellitus.Patients with type 2 diabetes mellitus was associated with more severity of lesion and more lesion vessel.

Objective:To observe the effects of osteoprotegerin on osteoclasts in the occlusal tissue during tooth eruption in dog and analyze the mechanism. Methods: Six 7-day-old brood dogs were divided randomly to control and osteoprotegerin groups. The dogs in osteoprotegerin group were injected with osteoprotegerin for 3 days 1.5 mg/(kg?d). 5 days after injection the dogs were sacrificed and mandible were taken out for paraffin slice. The number of the osteoclasts in the occlusal tissue up the third mandibular premolar was detected by tartrate-resistance acid phosphatase(TRAP) staining. Immunohistochemistry was used to examine the expressions of RANKL. Results: The number of osteoclasts in osteoprotegerin group was less than that in the control group (P

Objective To investigate the effect and mechanism of the icaritin on the human cronic myeloid leukemia K562 cells. Methods The K562 cells at logarithmic growth phase were divided into the control group and the icaritin group. The cells in the control group were normally treated and the cells in the icaritin group were incubated with 8 μmol/L icaritin. Methyl thiazolyl tetrazolium (MTT) method and flow cytometry were used to examine the proliferation and apoptotic changes in the two groups after incubation for 72 h, respectively. Gene expression of p85 and Akt were detected by RT-PCR. The protein changes of p85, Akt, p-p85, p-Akt cleavage-caspase-3 and caspase-3 were detected by Western blot. Results Compared with the control group, the proliferation rate of K562 cells in the icaritin group was significantly decreased (P<0.05), however the apoptotic rate of K562 cells and the expressions of p-p85, p-Akt, cleavage-caspase-3 in the icaritin group were significantly increased (all P< 0.05), but the expressions of p85 mRNA, Akt mRNA and caspase-3 protein had no difference (all P> 0.05). Conclusion Icaritin could induce the proliferation and promote the apoptosis of K562 cell, and its mechanism may be achieved through activating the PI3K-Akt signal transduction pathway.

Three-dimensional (3D) fluorescence spectra and thin layer fluorescence chromatogram of common Camptotheca fruit (CCF) crude drug, camptothecin (CPT) and 10-hydroxycamptothecin (HCPT) have been studied, and a novel fluorimetric method for determination of CPT in CCF crude drug has been established. In 3D fluorescence spectra, CPT presented 3 fluorescence peaks with excitation wavelengths lambdaex of 215, 255 and 365 nm, separately, and all peaks with emission wavelength lambdaem of 430 nm. HCPT presented 4 fluorescence peaks with lambdaex of 220, 265, 325 and 375 nm, separately, and all peaks with lambdaem of 555 nm. The fluorescence of CPT is much stronger than that of HCPT. Comparison of 3D fluorescence spectra and analysis of thin layer fluorescence chromatogram revealed that the main fluorescent component of CCF is CPT. HCPT and other components basically do not interfere with the fluorescence of CPT. Under the condition of pH 3.0-6.7, CCF aqueous solution can produce strong and steady fluorescence. Using methanol as solvent, the extracting solution of CCF was prepared, and diluted properly with water, then measured fluorescence intensity at lambdaex/lambdaem = 365/430 nm to determine the content of CPT. A linear calibration curve covered the concentration range 0.002 35-0.235 microg x mL(-1). The regression equation was IF = 9 + 30,844 c, with correlation coefficient r = 0.999 (n=9). The method has been applied to the analysis of CPT in a CCF sample, with a result of 0.127% and a spiked recovery rate of 102%. The reliability of the method has been verified by a thin layer chromatography-fluorescence scanning method. Experimental results demonstrated that this method can be used for quality evaluation of CCF crude drug.

AIM: To study the pharmacokinetics of ? Asarone in the volatile oil from Acorus. Tatarinowii Schott. in mice. METHODS: We developed a GC method for ? Asarone determination in blood. The GC system consisted of HP 35 capillary column with Helium as carrier gas, oven starting temperature set at 30℃ , then was raised to 220℃ at a rate of 6℃/min , stayed for 5 minutes, injector temperature set at 250℃ , detector temperature at 320℃ . Injection volume was 2.0 ?L with splitless inject mode. Methanol was used as solvent and ? naphthol as interior standard substance. CONCLUSION: The calibration curve in blood was linear in the range of 0.04?g/ml to 40?g/ml . The minimum detection limit was 0.04?g/ml . The recovery for ? Asarone in blood was 95.3% with RSD 3.92% . After oral administration of volatile oil from Acorus. Tatarinowii Schott., the intracorporal process of ? Asarone in mice accorded with 2 compartment model with 1st order absorption. 6.5min and 93.6min were obtained as t 1/2? and t 1/2? respectively.

Hip fractures are more common in the elderly,and the patients with conservative treatment have a high morbidity and mortality,and surgical treatment has become the preferred method.These patients are often characterized by a large number of comorbidities,a critical condition and high risks for anesthesia and surgery,and the choice of accurate preoperative risk assessment and the appropriate anesthesia methods will help to reduce postoperative complications and lead to better outcomes in elderly patients with hip fractures.

To introduce the necessity,contents and means of vocational training among fresh nurses (who work in the first year after graduation),to explore how to enhance the comprehensive professional qualitiy training mode for graduated nurses.

AIM: Based on the water-solubility of polyethylene glycol (PEG) with different relative molecular weight (RMW) and sodium alginate, we investigated the porous structure and property of calcium alginate scaffold with different RMW and dosages of PEG, which was used as porogenic agent. METHODS: The experiment was carried out in the Key Laboratory of Biomedical Engineering and Biomaterials, Jinan University from March 2006 to September 2007.①PEG at different RMWs (Mw=2 000, 4 000, 6 000, 8 000, 10 000, 20 000, 35 000) and different dosages (mass fraction=0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.06) were added into sodium alginate solution at 0.02 mass fraction. Then alginate was crosslinked, solidified and molded into indiscerptible film and graininess through Ca2+. PEG was dissolved by water. Therefore, a mass of porous structures could be formed in alginate.②Water content and swelling ratio of alginate was tested. Scanning electron microscope was used to observe the porous configuration. RESULTS: ①Porous alginate scaffold could be obtained through PEG and it exhibited good intensity and toughness, with water content reaching 92%. The scaffold could be formed into film and graininess.②Scanning electron microscope results revealed that the distribution of porous structure was uniformed. The aperture was 43.75 ?m-2.8 mm, and could be controlled by PEG's RMW and dosage. Inflated and uniform aperture structure was harvested when RMW of PEG was 4 000 and 6 000. CONCLUSION: Porous alginate structure can be obtained through regulating PEG's RMW and dosage. High water content and porosity of alginate scaffold material can be used in cell culture of tissue engineering and used as controlled release matrix of bio-active component.

0.05), while the values of BIS at all the time points in group A were significantly higher than those in groups B and C; meanwhile the BIS at different time points in group B were significantly higher than those in group C (both P