BACKGROUND:Pancreatic islet transplantationviaportal vein system leads to the apoptsis of a number of islet cels due to local hypoxia,therebyaffecting transplant outcomes. OBJECTIVE:To explore theeffect ofpre-micrvascularization network of tissue-engineered constructs on the survival of transplanted islets and the feasibility of xenogenic islet transplantation. METHODS:A 5-mm-long cylindrical silicone tube filed with Matrigel TM matrix surrounding the superficial epigastric vessel was placed in the groin ofdiabetic mice. After the syngeneic islets with 300 islet equivalents (IEQ) were transplanted into the silicone chamber on days 0, 14 and 28 post-chamber implantation, respectively, the recovery time of blood glucose was observed. The islets with the quantity of 100 IEQ, 200 IEQ and 300 IEQ, respectively, were transplanted on day 28 post-implantation and then the blood glucoselevelwas determined. Moreover, the survival of human pancreatic islets with 1 000 IEQ transplanted into the pre-vascularizated chamber or under the renal capsule of diabetic mice, folowed by the treatment of anti-CD45RB and/or anti-CD40L (MR-1) was analyzed. RESULTS AND CONCLUSION:An abundant micro-vascularized network was established in the silicone chamber on day 28 post-implantation. The time of the blood glucose returningto normal level in diabetic mice was negatively correlated with the time required for pre-vascularization and the number of implanted islets. No islet grafts implanted in the silicone chamber and treated by anti-CD45RB survived for long term. However, one of seven (14.3%) grafts survived for long term, which was not significantly different from the transplantation under the renal capsule group (n=8, MST > 71 days,P> 0.05). The tissue-engineered pre-vascularization network markedly extends the survival time of the islet grafts before transplantation. The transplantation of the xenogenic pancreatic islets into the vascularized silicone chamber might be a promising method in the future clinical application.

Asthma ; drug therapy ; Autoimmune Diseases ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Graft Rejection ; drug therapy ; Humans ; Immunosuppressive Agents ; therapeutic use ; Male ; Phytotherapy

Alcohol is widely abused in contemporary social life, which has become a serious medical and social problem because it hurts human health and endangers public safety. Recent re-search has developed several active substances that can effective-ly improve or treat this syndrome via affecting the mesolimbic do-pamine nervous pathway to dampen rewarding effectiveness in-duced by ethanol. This paper reviews the progress in near-term studies of alcoholism-intervening agents, aiming at providing ref-erences for related mechanism exploration and drug development.

Many reasons cause low back pain, such as muscles and ligaments injury, vertebral joints retrogression, spinal canal stenosis, lumbar disc herniation, lumbar spondylolisthesis, spondyloarthritis, infection, tumor and metabolic bone disease. It is in recent years that discogenic low back pain be recognised, especially after the MRI widely applied in clinics. This article makes a summary on discogenic low back pain of recent years from etiology, pathogenesis, diagnosis and therapy.

Purpose Placenta implantation (PI) is a rare but severe pregnancy complication, and imaging diagnosis is always difficult. This paper aims to explore the ideal fast-imaging MRI sequences for PI and to provide high quality images for diagnosis. Materials and Methods The MRI images of 21 cases with pathology confirmed PI were retrospectively analyzed. Prenatal MRI of 2D fast-imaging employing steady-state acquisition (2D FIESTA) and single-shot fast spin echo (SSFSE) sequences were performed, and the imaging quality rating and the accuracy in detecting PI were compared. Results Satisfactory images were acquired in 71.4% (15/21) of the patients using 2D FIESTA, and 38.1% (8/21) using SSFSE with statistically significant difference (χ2=4.790, P<0.05). The MRI features of PI included placenta heterogeneity, low-signal-intensity bands, abnormal placental vascularity, uterine junction zone interruption, placental tissue invading the myometrium, placenta tissue into uterine serosa, and the detection rates of 2D FIESTA and SSFSE sequences on the above features were 57.1%, 57.1%, 28.6%, 61.9%, 66.7% and 14.2%, respectively; 90.4%, 71.4%, 38.1%, 42.9%, 28.6% and 6.5%, respectively. The detection rates of placenta heterogeneity and placental tissue invading the myometrium using these two sequences are statistically different (χ2=4.560 and 6.109, P<0.05). Conclusion The images quality of 2D FIESTA sequence is higher than those of the SSFSE sequence. 2D FIESTA sequence shows better delineation of the border of the placenta and uterine, and SSFSE sequence shows better contrasts of the placenta;therefore, combining these two can improve MRI diagnostic value for PI.

Objective To observe the curative effect of acupuncture on insulin receptor substrate 1 ( IRS-1) , IRS-2 and glucose transporter 4 (GLUT4) in skeletal muscle of experimental rats with insulin resistance (IR) . Methods Thirty-two rats were randomly divided into normal group, model group, acupuncture normal group and acupuncture model group. The fasting plasma glucose ( FPG) level was detected with glucose oxidase method, fasting insulin and C-peptide ( C-P) were examined with enzyme linked immunosorbent assay ( ELISA) , insulin sensitivity index ( ISI) was calculated, and IRS-1 mRNA, IRS-2 mRNA and GLUT4 mRNA of skeletal muscle were observed by real-time PCR-based fluorescent assay in each group. Results Compared to the normal group, the FPG, FINS, C-P levels were increased dramatically (P<0.01), ISI, IRS-1 mRNA, IRS-2 mRNA and GLUT4 mRNA of the model group declined remarkably ( P<0.01). Compared to the model group, the FPG, FINS, C-P levels declined evidently ( P<0.05 or P<0.01) , and ISI, IRS-1 mRNA, IRS-2 mRNA and GLUT4 mRNA of the acupuncture model group were increased dramatically ( P<0.01). Conclusion The curative mechanism of acupuncture for insulin resistance is probably related to the increase of IRS-1 mRNA, IRS-2 mRNA and GLUT4 mRNA expression in skeletal muscle which contributes to the improvement of PI3K signal pathway.

Objective To evaluate the quality of life (QoL) in patients undergone intravesical chemotherapy for non-muscle invasive bladder cancer and analyze the correlation of the quality of life and low urinary tract symptoms in this group of patients.Methods 31 male patients and 15 female patients were enrolled from Jan.2012 to Mar.2013.The average age was 54 (35-71) years.Questionnaires of QoL and core lower urinary tract symptom score (CLSS) were given to 46 patients before intravesical chemotherapy and the 1 st,6th month after starting the instillation.Before the instillation,the scores of QoL and CLSS were 55.7±6.2 and 6.5±2.1,respectively,the change of QoL and CLSS were compared to baseline,and then the correlation of the low urinary tract symptoms and quality of life was analyzed.Results At the end of 1st and 6th month,the QoL scores were 74.7±8.1 and 78.5± 10.6.The CLSS scores were 8.9±2.0 and 9.1 ± 1.8,respectively.The differences were significant (P ＜0.05) when compared to the baselines.The areas of working activity and free time were affected much more than other areas,and the scores increased from 8.3±2.1 and 19.2±5.7 to 14.3±5.6 and 23.7±4.2,respectively.The area of free time was found to be associated with local symptoms (r=0.61).Conclusions Intravesical treatment can impair the QoL of patients.Low urinary tract symptom is an important factor and is associated with impairment of QoL,suggesting the relief of local symptoms may improve the overall quality of life.

Objective To estimate the quality and efficacy of the academic thesis of compound anisodine in traumatic optic neuropathies(TON)treatment. Method We searched Chinese database last or"blunt trauma"as key words,and analyzed them using the standard of evidence-based medicine(EBM).Result 6 RCTS with a total of 415 eyes included are retrieved,and the OR value is 6.54 with a 95%CI of[4.14,10.35],P＜0.00001,the difference is significant;sub-category analyses are made and both show significant difference(P＜0.0001). Conclusion The existing evidence supports that prognosis of TON is better when compound anisodine are adopt in treatment,and this effect is significant in steroid treatment.Compound anisodine can be used alone for TON treatment.However.because there are only 6 thesis are retrieved and all of them have methodological short-comings,the evidence is not convincing.There is an urgent need of well-planed,large-scale and multiple-center studies to assess the role of compound anisodine in traumatic optic neuropathies treatment.

Objective To explore the effect of Tie-2 small interference RNA(siRNA)treatment in human hepatoma transplanted subcutaneously in nude mice.Methods Tumor cells were implanted in the hind flank of male nude mice of 6 weeks.Tumor-bearing mice were divided into two groups(gene therapy group and control group)and injected intra-tumorally with Tie-2-siRNA/Lipofectamine and saline/Lipofectamine respectively.The tumor volume and weight,serum AFP and microvessel density(MVD)and the histological change of the tumor were tested after gene therapy.Results The growth inhibitory rates in gene therapy group were 26.94%,53.01% and 68.91% on day 4,7 and 10 after gene therapy respectively.The tumor volumes of gene therapy group(118.47,111.57 and 104.59 mm3)were smaller than those of the control group(162.17,237.46 and 336.41 mm3)respectively(P

Objective To investigate the elimination effect of propofol on the reactive oxygen species(ROS) in patients with acute craniocerebral injury (ACI). Methods Forty patients with ACI were randomly divided into propofol group receiving propofol anesthesia and ?-OH group as control . Electron spin resonance(ESR) spectroscopy was used to determine the plasma contents of oxygen free radical (OFR), and the plasma contents of lipid peroxides(LPO) and nitrogen oxide (NO) were determined with chemical method. Blood samples were collected before anesthesia and 2, 4 hours after starting operation. Results The plasma contents of NO, OFR and LPO significantly increased before operation in patients with ACI compared with healthy subjects(all P