Atherectomy, Coronary ; methods ; Drug-Eluting Stents ; Humans

Objective To observe the clinical efficacy of Yang Xin Huo Xue(nourishing the heart and activating blood flow) needling method plus Western medication in treating chronic heart failure due to coronary heart disease (CHD).Method Eighty patients with CHD-induced chronic heart failure were randomized into a treatment group and a control group, to respectively receiveYang Xin Huo Xueneedling method plus Western medicationor Western medication alone for intervention. The heart function was estimated and brain natriuretic peptide (BNP) was detected before and after the intervention in both groups, and the change of heart function was observed.Result Yang Xin Huo Xueneedling method plus Western medication achieved a satisfactory therapeutic efficacy in treating chronic heart failure, and its total effective rate was significantly different from the rate in the control group (P<0.05); compared with the control group, the BNP value declined more significantly in the treatment group, and the difference was statistically significant (P<0.05).Conclusion Yang Xin Huo Xueneedling method plus Western medication can produce a satisfactory efficacy in treating CHD-induced chronicheart failure.

Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate,The in- hibiton of cell proliferation,cell cycle distribution,expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay,flow cytometry,immunocytochemistry,and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24,48,72 h after intervention were 2.41?0.48,0.72?0.08,and 0.39?0.04 ug/ml,The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle,enhancement of Caspase-3,and inhibition of Survivin.

A L-serine producing facuhative methylotroph mutant (Mth~H、Gly~R)was derived from Pseudomonas flava A3 by combination treatment with ultraviolet(UV)and diethylsulfate(DES).It could accumulate 6.2g/L L-serine in the medium containing 30g/L glycine and 1% methanol as carbon source when it was cultured for 3 days,and it has an improvement about 67.6% compared with the origin.The mutant also has a good stability of descendiblity of L-serine producing.

Exploring the functional network during the interaction between emotion and cognition is an important way to reveal the underlying neural connections in the brain. Sparse Bayesian network (SBN) has been used to analyze causal characteristics of brain regions and has gradually been applied to the research of brain network. In this study, we got theta band and alpha band from emotion electroencephalogram (EEG) of 22 subjects, constructed effective networks of different arousal, and analyzed measurements of complex network including degree, average clustering coefficient and characteristic path length. We found that: (1) compared with EEG signal of low arousal, left middle temporal extensively interacted with other regions in high arousal, while right superior frontal interacted less; (2) average clustering coefficient was higher in high arousal and characteristic path length was shorter in low arousal.
Arousal ; Bayes Theorem ; Brain ; physiology ; Brain Mapping ; Cluster Analysis ; Cognition ; Electroencephalography ; Emotions ; Humans

Fracture Fixation ; instrumentation ; methods ; Fractures, Bone ; surgery ; Humans ; Scaphoid Bone ; injuries ; surgery ; Wrist Injuries ; surgery ; Wrist Joint ; surgery

Metabolic memory means if the hyperglycemia can't be controlled at early stage of diabetes,chronic complications such as diabetic retinopathy (DR) will continue to develop even if the blood glucose level maintains normal level at later stage.Oxidative stress plays an important role in the metabolic memory of DR,which interacts with the nitrative stress,advanced glycation end products,genetic modification and endoplasmic reticulum stress in the pathogenesis of DR.Further elucidation of the relationship between oxidative stress and metabolic memory of DR can open the way for the discovery of novel therapeutic targets to prevent DR progression.

Objective To understand the epidemiological characteristics of bacillary dysentery and etiological characteristics of Shigella in Tianjin, and to provide evidence for the prevention and control measures. Methods The data of disease surveillance information reporting system from 2008 to 2014 were analyzed retrospectively. A total of 3955 stool samples came from the clinically diagnosed cases of bacillary dysentery were collected, according to the extraction method of stratified sampling in Tianjin city, suburban area, Binhai New Area and suburban area sentinel hospitals, and according to the sampling interval for the May to October from 2008 to 2014. Samples were detected and cultured for Shigella flora. Results (1) Epidemiological characteristics:from 2008 to 2014, a total of 65179 cases of bacillary dysentery were reported in Tianjin city, with an average annual incidence of 72.00/100 thousand. The annual incidence rate showed a downward trend year by year. The incidence was a downward trend, including urban (120.28/100 thousand), suburban areas (70.36/100 thousand), Binhai New Area (64.22/100 thousand) and the outer suburbs (19.39/100 thousand). The onset time was mainly concentrated in June-September, and the peak incidence occurred in August. The incidence rate was higher in people below 5 years old and above the age of 85. The annual incidence rate was higher in male patients of 0 to 24 years old and above 75 years old than that of female. The annual incidence rate was higher in female patients of 50 to 74 years old age group than that of male (P<0.05). The distribution of occupation was scattered among children and retirees. (2) Distribution of bacteria:the 229 Shigella strains were positive (5.79%), including 136 strains of Shigella sonnei (59.39%), 93 strains of Shigella flexneri (40.61%). The 89 strains of Shigella flexneri (4 strains were not detected) were detected in 8 subtypes, F2a (49.44%), F2b (22.47%) and FX (13.48%). The subtypes of Shigella flexneri were detected decreased year by year from 2008 to 2014, showing a single subtype. The highest detection rate of Shigella was found in 10-19 years old (9.55%). The S.sonnei positive rate was the highest in urban (5.09%), and the Shigella flexneri positive rate was the highest in suburban areas (3.09%). Conclusion The key population for the prevention and control of bacillary dysentery in Tianjin is scattered children and old people. It is necessary to strengthen the propaganda and health education to improve the health consciousness of the key population and their daily nursing staff. With the continuous changes of Shigella flora, it is necessary to carry out long-term monitoring to find out the regularity of shigella.

Objective To study the regulatory effect of interleukin-17 on the production of inflammation and bone erosion related factors of fibroblast-like synoviocytes (FLSs) from rheumatoid arthritis (RA) patients and to investigate whether signal transducer and activator of transcription 3 (STAT3) is implicated in this regulatory effect.Methods FLSs were acquired through primary culture from RA patients.Receptor activator for nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) expression in FLSs were determined using western blot analysis.The level of IL-6,granulocyte-mac rop hage colony stimulating fac tor (GM-CSF)and IL-8 were determined by enzyme linked immunosorbent assay (ELISA) assay.Statistical product and service solutions (SPSS) 17.0 statistical software was used for single factor analysis of variance and LSD-t comparison between each two groups.Results IL-17 stimulated FLSs and promoted the release of IL-6,GM-CSF and IL-8 (t=2.135,P＜0.01) and increased the production of RANKL (t=2.105,P＜0.01) and reduced the production of OPG (t=2.502,P＜0.01).However,targeted silencing of STAT3 could inhibit the effect of IL-17 on FLSs [control group:IL-6 (512±18) pg/ml;GM-CSF (591±25) pg/ml;IL-8 (473±10) pg/ml;RANKL (2.310±0.096);OPG (0.614±0.311);IL-17 group:IL-6 (1 630±39) pg/ml;GM-CSF (1 825±18) pg/ml;IL-8 (2 804±41) pg/ml;RANKL (4.010±0.256);OPG (0.131±0.101);STAT3 silent group:IL-6 (405±21) pg/ml;GM-CSF (451±12) pg/ml;IL-8 (370±9) pg/ml;RANKL (0.850±0.298);OPG (1.120±0.342)].Conclusion STAT3 pathway is critical to IL-17-induced regulatory effect on the release of inflammation and bone erosion related factors of FLSs.IL-17 can induce the inflammation and bone erosion of RA via STAT3.

Objective:The purpose of the study was to investigate the effects of enteral nutrition on stress response and gut barrier function after surgery for esophageal cancer. Methods: Patients who underwent esophagectomy were divided into two groups. Group PN (28 patients) served as the control group. Group EN (26 patients) received enteral nutrition. Results: The serum total protein, albumin, prealbumin and transferrin decreased after operation, but there were no differences between the two groups. The serum levels of IL-6 were significantly higher on the first and third postoperative days after the operation in two groups (P