Atherectomy, Coronary ; methods ; Drug-Eluting Stents ; Humans

Objective:To observe the inhibitory effect of disodium cantharidinate on human hepatoma cell line HepG2 and investi-gate its possible mechanisms. Methods:HepG2 cells were treated with different concentration of disodium cantharidinate,The in- hibiton of cell proliferation,cell cycle distribution,expression of Survivin and activity of Caspase-3 were respectively evaluated by MTT assay,flow cytometry,immunocytochemistry,and chromatometry. Results:Disodium cantharidinate could inhibit the proliferation of HepG2 cells. The IC50 at 24,48,72 h after intervention were 2.41?0.48,0.72?0.08,and 0.39?0.04 ug/ml,The cells of G2/M phase and activity of Caspase-3 were increased.the expression respectively of Survivin were inhibited after the intervention of disodium cantharidinate. Conclusion:The inhibitory effect of disodium cantharidinate on HepG2 cells may be mediated by the block of cellcycle,enhancement of Caspase-3,and inhibition of Survivin.

Objective To observe the clinical efficacy of Yang Xin Huo Xue(nourishing the heart and activating blood flow) needling method plus Western medication in treating chronic heart failure due to coronary heart disease (CHD).Method Eighty patients with CHD-induced chronic heart failure were randomized into a treatment group and a control group, to respectively receiveYang Xin Huo Xueneedling method plus Western medicationor Western medication alone for intervention. The heart function was estimated and brain natriuretic peptide (BNP) was detected before and after the intervention in both groups, and the change of heart function was observed.Result Yang Xin Huo Xueneedling method plus Western medication achieved a satisfactory therapeutic efficacy in treating chronic heart failure, and its total effective rate was significantly different from the rate in the control group (P<0.05); compared with the control group, the BNP value declined more significantly in the treatment group, and the difference was statistically significant (P<0.05).Conclusion Yang Xin Huo Xueneedling method plus Western medication can produce a satisfactory efficacy in treating CHD-induced chronicheart failure.

A L-serine producing facuhative methylotroph mutant (Mth~H、Gly~R)was derived from Pseudomonas flava A3 by combination treatment with ultraviolet(UV)and diethylsulfate(DES).It could accumulate 6.2g/L L-serine in the medium containing 30g/L glycine and 1% methanol as carbon source when it was cultured for 3 days,and it has an improvement about 67.6% compared with the origin.The mutant also has a good stability of descendiblity of L-serine producing.

Objective To determine the effect of interleukin (IL)-17 on the proliferation of fibroblastlike synoviocytes (FLSs) from rheumatoid arthritis (RA)patients and to investigate whether signal transducer and activator of transcription 3 (STAT3) was implicated in this process.Methods FLSs were acquired by primary culturing from RA patients.STAT3,p-STAT3,Bcl-2 and Bax expression in FLSs were determined using western blotting analysis.The proliferation of FLSs was determined by MTT assay.Analysis of variance was used to determine the differences between two groups.Results IL-17 promoted the proliferation of FLSs from RA patients and inhibited their apoptosis.The pro-apoptotic Bax was decreased and anti-apoptotic Bcl-2 was increased in FLSs from RA (t=2.612,P＜0.05;t=2.723,P＜0.05).However,targeted silencing of STAT3 could inhibit the effect of IL-17 on FLSs (t=2.745,P＜0.05).[the control group:Bax (3.814±1.623),Bcl-2 (1.429± 0.311);IL-17 group:Bax (0.972±0.017),Bcl-2 (3.175±1.356);STAT3 silent group:Bax (5.729±1.236),Bcl-2 (0.637±0.006)].Conclusion STAT3 is critical in IL-17-induced proliferation of FLS from RA patients.IL-17/STAT3 pathway might be a good candidate for RA treatment modalities.

Objective　To study the factors effecting the formation of the ventricular aneurysm post myocardial infarction (MI) and evaluate the diagnostic methods.Methods　23 MI patients with ventricular aneurysm confirmed by left ventricular angiography were observed for electrocardiogram (ECG), echocardiogram (ECHO), coronary disease and collateral vessels formation. Results　All patients had LAD disease, 65.2% patients had three coronary disease, and those with class Ⅲ collateral vessels circulation only 17.4%. Compared to ECHO by which only 4 patients were found aneurysm, the positive rate by ECG was 82.6%. Conclusion　Patients with multiple coronary disease and without enough collateral vessels are expected to form ventricular aneurysm. ECG is more sensitive than ECHO for diagnosis of ventricular aneurysm post MI

Colorectal neuroendocrine neoplasm (NEN) is originated from colorectal neuroendocrine cells.The incidence of colorectal NEN is low,however it has been increasing in recent years.As the research work has gone further,WHO has graded colorectal NEN to neuroendocrine tumor,neuroendocrine carcinoma,mixed gland-neuroendocrine carcinoma and hyperplastic and pre-neoplastic lesions.Although colorectal NEN cells can produce hormones and have endocrine functions,the clinical symptoms may not be shown.The diagnosis and treatment for colorectal NEN are different according to different classifications and stages.

Objective To understand the clinical characteristics of lower respiratory tract infection,pathogenic distribution and drug resistance in intensive care unit to provide a basis for reasonable use of antibiotics. Methods From January 2008 to August 2010,the clinical data from our hospital ICU impatients of lower respiratory tract infection were retrospectively analyzed. The isolated strains were identified, the antibiotic susceptibility test was performed by K-B methods and the results were read according to CLSI 2007. Results There were 509 patients, 147 cases of lower respiratory tract infection were found. Lower respiratory tract infection rate was 28.9% (147/509); 283 strains of pathogens had been isolated by culture and most of which were Gram negative bacilli(86. 9%), the other pathogens included Gram positive cocci(7.4%) and fungi(5.7%). The most common pathogens were Acinetobacter spp, Pseudomonas aeruginosa, Klebsiella pneumoniae,Stenotrophomonas oligotrophic aeromons, Fungi, Escherichia coli, Staphylococcus aureus, Burkholderia cepacia,etc,accounting for 24. 7% ,20. 5% ,12. 7% ,6. 4% ,5.7% ,5. 3% ,5.3% and 4. 6% ;The main pathogens were highly resistant to the most antibiotics. Conclusion ICU had a higher lower respiratory tract infection rate, Gram negative bacilli are the main pathogen, and showed the multi-drug resistance features to the antibiotics.

There are 10％-25％ inherited breast cancer patients among all breast cancer patients.Hereditary factors play an important role in the occurrence and development of inherited breast cancer.There are three kinds of susceptibility genes of inherited breast cancer including high penetrance susceptibility genes,middle penetrance susceptibility genes and low penetrance susceptibility genes.Various susceptibility genes are closely related to inherited breast cancer.